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A visible-light-excitable Eu3+ complex-based luminescent probe, [Eu(pdap)3(DPBT)], has been proposed for time-gated luminescence imaging of singlet oxygen in the mitochondria of living cells, as well as in tumor tissues and laboratory animals. Extension of the excitation window to the visible-light region makes the probe more favorable for practical usage. [ABSTRACT FROM AUTHOR]

The Golgi apparatus is a crucial component of the endomembrane system and is involved in various physiological and pathological processes. Recently, increasing evidence shows that Golgi participates in oxidative stress in sepsis-induced acute lung injury (ALI). As a kind of reactive oxygen species (ROS), peroxynitrite (ONOO−) plays a significant role in a range of oxidative stress-related diseases. Therefore, accurately monitoring the level of ONOO− in the Golgi apparatus is crucial for revealing the mechanism of Golgi ONOO− in the sepsis-induced ALI. Unfortunately, no such probe has been reported to detect Golgi ONOO− in sepsis-induced ALI model. In this work, a Golgi-targetable probe FC-ONOO was designed and synthesized for high sensitivity and selectivity monitoring ONOO− generation during the Golgi stress response in sepsis-induced ALI model. The probe displayed high sensitivity (about 57 nM of detection limit) and good selectivity towards ONOO−. Additionally, it was successfully applied in mapping the increase of ONOO− levels in the Golgi of live cells induced by different stimuli. More importantly, the substantial increases in ONOO− levels in the Golgi apparatus were observed in the sepsis-induced ALI of mice for the first time. Given all its outstanding advantages, it has great potential for application in other ONOO− related diseases. [Display omitted] • A novel Golgi-targetable fluorescent probe for ONOO− was developed. • The probe displayed high sensitivity and good selectivity towards ONOO−. • The probe was successfully used to track Golgi ONOO− produced in live cells induced by different stimuli. • The probe was successfully applied to bioimaging Golgi ONOO− fluxes during sepsis-induced ALI for the first time. [ABSTRACT FROM AUTHOR]

Ferroptosis is a form of programmed cell death mostly characterized by a serious misadjustment in the production and degradation of lipid reactive oxygen species (ROS). Meanwhile, numerous studies have indicated that ferroptosis is crucial to the progression and pathophysiology of acute kidney injury (AKI). ROS have been implicated as a pivotal causative factor in cell damage, peroxynitrite (ONOO−) is one of the most prominent ROS which is intimately correlated to ferroptosis-mediated AKI. Therefore, monitoring ONOO− levels in ferroptosis-mediated AKI is important for exploring its physiological and pathological roles. Nevertheless, no probes for detecting ONOO− in ferroptosis-mediated AKI have been reported. Meanwhile, resveratrol (Res) is a bioactive component and has been used to the treatment of AKI. However, the effect of Res on ferroptosis during AKI progression remains elusive. Herein, we evaluated the fluctuation of ONOO− during ferroptosis-mediated AKI using the near-infrared (NIR) fluorescence probe (NFP-ONOO) for the first time. The probe NFP-ONOO showed excellent specificity and sensitivity for the detection of ONOO−. Furthermore, utilizing this probe, we demonstrated Res effectively mitigates AKI induced by folic acid (FA) and cisplatin (CP) by inhibiting ferroptosis. Collectively, this work will provide a powerful guidance for the prophylaxis, diagnosis, and therapy of ferroptosis-mediated AKI. [Display omitted] • A novel near-infrared fluorescent probe NFP-ONOO for ONOO− was fabricated. • The probe NFP-ONOO displayed high selectivity and sensitivity toward ONOO−. • NFP-ONOO was used for imaging ONOO− in cells and zebrafish during ferroptosis. • NFP-ONOO was utilized to image ONOO− in ferroptosis-mediated AKI models. [ABSTRACT FROM AUTHOR]

Development of fluorescent/luminescent probes for rapid, selective and sensitive detection of reactive oxygen species (ROS) is of great significance for understanding the roles of ROS in pathophysiological processes. In the present research, a visible-light-excitable Eu3+ complex-based probe, Eu(L) 3 (DPBT), is designed and synthesized for the time-gated luminescence (TGL) determination of hypochlorous acid (HClO) in vitro and in vivo. The proposed probe exhibits a rapid, selective and sensitive TGL response to HClO, and excellent localization of mitochondria in living cells with low cytotoxicity. These features allow the probe to be used for the TGL sensing and imaging of HClO formation in mimic inflammatory cells at a subcellular level, as well as in endotoxin-induced liver injury and rheumatoid arthritis in live mice. In addition, by immobilizing the probe in the PEG hydrogel, the smart sensor films with rapid response to HClO were prepared, and successfully used for the real-time monitoring of HClO generation in mouse wounds, in order to distinguish the infected wounds from acute ones. Overall, this study provides a useful tool for the clinical monitoring and treatment of wound diseases. • A novel mitochondria-targetable time-gated luminescence probe for HClO was developed. • The as-prepared Eu3+ complex-based luminescence probe possesses a broad excitation window including visible light. • The probe can be used for imaging HClO in mitochondria of living cells, as well as in liver injury and RA mouse models. • The probe-doped smart sensor films were successfully used for the real-time monitoring of HClO generation in mouse wounds. [ABSTRACT FROM AUTHOR]