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To investigate the expressions of caspase-8, receptor interacting protein (RIP) and nuclear factor (NF)-kappaBp65 in oral lichen planus (OLP) and their relationship with cell apoptosis.Immunohistochemical technique with SP method was used to detect the expressions of caspase-8, RIP and NF-kappaBp65 in 30 OLP cases and 15 normal oral mucosa specimens. Terminal deoxynucleotidyl transferase-mediated nucleotide shift enzyme (TdT) mediated d-UTP end labeling (TUNEL) was used for detecting the cell apoptotic index (AI) in 15 OLP cases and 5 nomal oral mucosa specimens.Compared with the control group, the AI of epithelial cells (6.76 +/- 2.32) increased and the AI of lymphocytes (1.75 +/- 0.74)decreased in OLP (P0.01). The positive rate of caspase-8, RIP and NF-kappaBp65 of epithelial cells were 97% (29/30), 87% (26/30) and 93% (28/30) respectively, significantly higher in OLP than in normal control (P0.05). The positive rate of caspase-8, RIP and NF-kappaBp65 of lymphocytes were 100% (30/30, 90% (27/30) and 80% (24/30) respectively, significantly higher in OLP than in normal control (P0.01). A positive correlation was also observed between NF-kappaBp65 expression of lymphocytes and AI of epithelial cells.Accelerated apoptosis of the keratinocytes and inhibition of lymphocyte apoptosis may coexist and contribute to the formation and progression of OLP. The over expression of caspase-8, RIP and NF-kappaBp65 in OLP may play a role in the pathogenesis of OLP.

To detect the expressions of receptor-interacting protein (RIP) and caspase-8 and investigate their roles in oral squamous cell carcinoma (OSCC) and oral precancerous lesions.SABC immunohistochemical methods were used to detect the expressions of RIP and caspase-8 in 22 specimens of OSCC, 14 specimens of oral lichen planus (OLP), 14 specimens of oral leukoplakia (OLK) and 10 specimens of normal oral mucosa (NOM).The rate of weak or negative expression of RIP in normal mucosa was 50% (5/10). The rates of weak and positive expression of RIP in OLP, OLK and OSCC were 75% (36/50), and the rate of positive and strong expression of RIP was 63.7% (14/22) in OSCC, significantly higher that in the others groups (P0.05). The rates of weak, positive and strong positive expression of caspase-8 in NOM, OLP, OLK and OSCC were 80% (8/10), 100% (14/14), 85.7% (12/14), and 100% (22/22), respectively.Both RIP and caspase-8 may play important roles in the occurrence and progression of OSCC and oral precancerous lesions.