1. Interaction of neuropeptide Y receptors (NPY 1 , NPY 2 and NPY 5 ) with somatostatin on somatostatin-induced feeding behaviour in neonatal chicken.
- Author
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Yousefvand S, Hamidi F, Zendehdel M, and Parham A
- Subjects
- Animals, Animals, Newborn genetics, Animals, Newborn physiology, Avian Proteins antagonists & inhibitors, Avian Proteins metabolism, Chickens genetics, Eating drug effects, Eating genetics, Injections, Intraventricular veterinary, Male, Random Allocation, Receptors, Neuropeptide Y antagonists & inhibitors, Receptors, Neuropeptide Y metabolism, Somatostatin administration & dosage, Avian Proteins genetics, Chickens physiology, Feeding Behavior drug effects, Receptors, Neuropeptide Y genetics, Somatostatin pharmacology
- Abstract
1. The present study was conducted to investigate whether brain somatostatin increases feed intake in neonatal chickens. The mediating role of neuropeptide Y receptors on feed intake induced by somatostatin was investigated. 2. In this study, seven experiments were designed, each with four treatment groups (n = 44 in each experiment). In Experiment 1, chicks received control solution and 0.5, 1 and 2 nmol of somatostatin through intracerebroventricular (ICV) injection. In experiments 2, 3 and 4, chickens were ICV injected with control solution and 1.25, 2.5 and 5 μg of B5063 (NPY
1 receptor antagonist), SF22 (NPY2 receptor antagonist) and SML0891 (NPY5 receptor antagonist), respectively. In experiment 5, 6 and 7 chickens received ICV injection of B5063, SF22, SML0891, with a co-injection of + somatostatin, control solution and somatostatin. The cumulative feed intake was measured until 120 min post injection. 3. Somatostatin significantly increased feed intake in FD3 chicks. Both B5063 and SML0891 dose-dependently decreased feed intake compared with the control group, while SF22 led to a dose-dependent increase in feed intake. In addition, the hyperphagic effect of somatostatin significantly decreased with co-injection of B560 plus somatostatin (p < 0.05), but SF22 and SML0891 had no effect on feed intake induced by somatostatin in chicks (p > 0.05). 4. Based on the results of this study, it is likely that somatostatin increased feed intake and NPY1 receptor acts as a mediator in hyperphagic effect of somatostatin in neonatal chicks.- Published
- 2019
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