1. [Study of the receptor for black widow spider neurotoxin. II. Isolation and characteristics of the receptor from bovine brain membranes].
- Author
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Petrenko AG, Surkova IN, Shamotienko OG, Kovalenko VA, Krasnoperov VN, Tret'iakov LA, Ushkarev IuA, and Grishin EV
- Subjects
- Animals, Cattle, Chromatography, Affinity, Electrophoresis, Polyacrylamide Gel, Kinetics, Membranes metabolism, Molecular Weight, Receptors, Cholinergic metabolism, Arthropod Venoms metabolism, Brain Chemistry, Neurotoxins metabolism, Receptors, Cholinergic isolation & purification, Receptors, Peptide, Spider Venoms metabolism
- Abstract
Biospecific sorbents for isolation of the latrotoxin receptor have been obtained and studied. Binding of the receptor components solubilized from the bovine cerebral cortex membrane to the immobilized toxin critically depends on the presence of calcium ions and the solution ionic strength. The procedure for semipreparative isolation of the highly active receptor preparation with Kd = 9 x 10(-10) M and Bmax = 0.9 nmol/mg has been developed. Binding activity of the isolated receptor is inhibited by heating as well as by proteases and denaturing agents. According to electrophoretic analysis in the presence of SDS the receptor complex contains protein components of molecular mass 200, 160, 79, 43 kDa, the former two being glycoproteins.
- Published
- 1990