1. Comparison of different immunization routes on the immune responses induced by Mycobacterium tuberculosis ESAT-6/CFP-10 recombinant protein.
- Author
-
Namvarpour M, Tebianian M, Mansouri R, Ebrahimi SM, and Kashkooli S
- Subjects
- Administration, Intranasal, Animals, Antigens, Bacterial genetics, Bacterial Proteins genetics, Female, Immunity, Cellular drug effects, Immunity, Cellular immunology, Immunity, Humoral drug effects, Immunity, Humoral immunology, Injections, Intramuscular, Injections, Subcutaneous, Mice, Mycobacterium tuberculosis drug effects, Tuberculosis microbiology, Tuberculosis prevention & control, Tuberculosis Vaccines administration & dosage, Vaccination methods, Antigens, Bacterial immunology, Bacterial Proteins immunology, Mycobacterium tuberculosis immunology, Recombinant Proteins immunology, Tuberculosis immunology, Tuberculosis Vaccines immunology
- Abstract
According to some difficulties against tuberculosis (TB) vaccination, development of new TB vaccines has been noted in recent years. Selection of proper route for vaccination is one of the most important factors for induction of good immune responses. Hence, in this study, the effects of different administration routes, including intranasal (I.N), subcutaneous (S.C) and intramuscular (I.M) on immune responses against Mycobacterium tuberculosis ESAT-6/CFP-10 recombinant protein has been considered. Recombinant ESAT-6/CFP-10 protein with or without adjuvant (MF59 or cholera toxin B (CTB)) was administered by three routes of I.M, I.N and S.C to mice for three times. Then, the levels of specific antibodies, lymphocyte proliferation and IFN-γ/IL-5 cytokine profile have been carried out to evaluate the humoral and cellular responses. The results showed that the titers of specific antibodies were quickly elevated in S.C and I.M groups after first immunization. Otherwise, the raise of antibody has delay in the I.N immunized animals. The levels of IFN-γ and lymphocyte proliferation have been increased in all of vaccinated groups. However, the I.N immunized mice have lower levels of IL-5 production. Based on our finding, the ESAT-6/CFP-10 recombinant protein is a potent stimulator of immune responses in all of three immunization strategies. However intranasal administration of this antigen has tended to reinforcement of cellular immune responses., (Copyright © 2019 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF