Search

Your search keyword '"Jia, La"' showing total 4 results
Start Over Author "Jia, La" Topic reperfusion injury
4 results on '"Jia, La"'

1. Hyperbaric oxygen preconditioning inhibits skin flap apoptosis in a rat ischemia-reperfusion model.

Author

Xiao YD, Liu YQ, Li JL, Ma XM, Wang YB, Liu YF, Zhang MZ, Zhao PX, Xie F, and Deng ZX

Subjects
Animals, Caspase 3 metabolism, Disease Models, Animal, MAP Kinase Kinase Kinase 5 metabolism, Male, Proto-Oncogene Proteins c-bcl-2 metabolism, Random Allocation, Rats, Sprague-Dawley, Reperfusion Injury metabolism, Reperfusion Injury pathology, Skin metabolism, Skin pathology, bcl-2-Associated X Protein metabolism, Apoptosis, Hyperbaric Oxygenation, Ischemic Preconditioning methods, Reperfusion Injury prevention & control, Skin blood supply
Abstract

Background: Hyperbaric oxygen (HBO) improves skin flap function and inhibits partial necrosis induced by ischemia-reperfusion (I/R) injury. Our study aimed to evaluate the mechanism underlying HBO regulation of the antiapoptosis factors associated with I/R injury of skin flaps., Methods: The rats were divided into sham surgery, I/R, and HBO groups. Rats from the HBO group received HBO preconditioning followed by I/R surgery. Blood perfusion of the skin flaps was measured with laser Doppler flowmeters. Tissue morphology and apoptosis were subsequently assessed based on hematoxylin-eosinhe and terminal deoxynucleotidyl transferase dUTP nick-end labeling staining. Protein expression of phosphorylated apoptosis signal-regulating kinase 1 (pASK-1), phosphorylated c-Jun N-terminal kinase (pJNK), B-cell lymphoma-2 (Bcl-2), and Bcl2-associated X protein (Bax) was examined by immunodetection, and Bcl-2 messenger RNA expression was detected by quantitative polymerase chain reaction. In addition, caspase-3 activity was also measured., Results: The result of microcirculation analysis showed that the survival and blood perfusion rates significantly increased in the skin flap after HBO exposure. Terminal deoxynucleotidyl transferase dUTP nick-end labeling staining revealed that cell apoptosis was significantly attenuated in the HBO group. Furthermore, HBO preconditioning increased the expression of Bcl-2 and inhibited pASK-1, pJNK, and Bax expression as determined by both immunohistochemistry and Western blot. Caspase-3 activity and the Bax/Bcl-2 ratio declined in the HBO group., Conclusions: HBO preconditioning effectively ameliorates I/R injury by regulating the apoptosis signal-regulating kinase 1 and/or c-Jun N-terminal kinase pathway and anti- and proapoptosis factors., (Copyright © 2015 Elsevier Inc. All rights reserved.)

Published
2015
Full Text
View/download PDF

2. Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway.

Author

Liu YQ, Liu YF, Ma XM, Xiao YD, Wang YB, Zhang MZ, Cheng AX, Wang TT, Li JL, Zhao PX, Xie F, and Zhang X

Subjects
Animals, Apoptosis genetics, Free Tissue Flaps blood supply, MAP Kinase Kinase Kinase 5 metabolism, Male, Rats, Rats, Sprague-Dawley, bcl-2-Associated X Protein metabolism, Apoptosis drug effects, Hydrogen administration & dosage, Ischemia prevention & control, Proto-Oncogene Proteins c-bcl-2 metabolism, Reperfusion Injury prevention & control, Saline Solution, Hypertonic administration & dosage, Skin blood supply
Abstract

Introduction: Many pathways have been reported involving the effect of hydrogen-rich saline on protecting skin flap partial necrosis induced by the inflammation of ischemia/reperfusion injury. This study focused on the influence of hydrogen-rich saline treatment on apoptosis pathway of ASK-1/JNK and Bcl-2/Bax radio in I/R injury of skin flaps., Methods: Adult male Sprague-Dawley rats were divided into three groups. Group 1 was sham surgery group, Group 2 and 3 were ischemia/reperfusion surgery treated with physiological saline and hydrogen-rich saline respectively. Blood perfusion of flap was measured by Laser doppler flowmeters. Hematoxylin and eosin staining was used to observe morphological changes. Early apoptosis in skin flap was observed through TUNEL staining and presented as the percentage of TUNEL-positive cells of total cells. pASK-1, pJNK, Bcl-2 and Bax were examined by immunodetection. In addition Bcl-2, Bax and caspase-3 were detected by qPCR. Caspase-3 activity was also measured., Results: Compared to the Group 2, tissues from the group 3 were observed with a high expression of Bcl-2 and a low expression of pASK-1, pJNK, and Bax, a larger survival area and a high level of blood perfusion. Hydrogen-rich saline ameliorated inflammatory infiltration and decreased cell apoptosis., Conclusion: The results indicate that hydrogen-rich saline could ameliorate ischemia/reperfusion injury and improve flap survival rate by inhibiting the apoptosis factor and, at the same time, promoting the expression of anti-apoptosis factor., (Copyright © 2015. Published by Elsevier Ltd.)

Published
2015
Full Text
View/download PDF

3. Hyperbaric oxygen preconditioning inhibits skin flap apoptosis in a rat ischemia-reperfusion model

Author

Mingzi Zhang, Jia-La Li, Yifang Liu, Zi-xuan Deng, Fei Xie, Yi-Ding Xiao, Xuemei Ma, You-Bin Wang, Yun-Qi Liu, and Pengxiang Zhao

Subjects
Male, Pathology, medicine.medical_specialty, Necrosis, Ischemia, Apoptosis, Pharmacology, MAP Kinase Kinase Kinase 5, Microcirculation, Rats, Sprague-Dawley, Random Allocation, Western blot, medicine, Animals, Ischemic Preconditioning, Skin, bcl-2-Associated X Protein, Hyperbaric Oxygenation, medicine.diagnostic_test, business.industry, Kinase, Caspase 3, medicine.disease, Disease Models, Animal, Terminal deoxynucleotidyl transferase, Proto-Oncogene Proteins c-bcl-2, Reperfusion Injury, Surgery, medicine.symptom, business, Perfusion
Abstract

Background Hyperbaric oxygen (HBO) improves skin flap function and inhibits partial necrosis induced by ischemia–reperfusion (I/R) injury. Our study aimed to evaluate the mechanism underlying HBO regulation of the antiapoptosis factors associated with I/R injury of skin flaps. Methods The rats were divided into sham surgery, I/R, and HBO groups. Rats from the HBO group received HBO preconditioning followed by I/R surgery. Blood perfusion of the skin flaps was measured with laser Doppler flowmeters. Tissue morphology and apoptosis were subsequently assessed based on hematoxylin-eosinhe and terminal deoxynucleotidyl transferase dUTP nick-end labeling staining. Protein expression of phosphorylated apoptosis signal-regulating kinase 1 (pASK-1), phosphorylated c-Jun N-terminal kinase (pJNK), B-cell lymphoma-2 (Bcl-2), and Bcl2-associated X protein (Bax) was examined by immunodetection, and Bcl-2 messenger RNA expression was detected by quantitative polymerase chain reaction. In addition, caspase-3 activity was also measured. Results The result of microcirculation analysis showed that the survival and blood perfusion rates significantly increased in the skin flap after HBO exposure. Terminal deoxynucleotidyl transferase dUTP nick-end labeling staining revealed that cell apoptosis was significantly attenuated in the HBO group. Furthermore, HBO preconditioning increased the expression of Bcl-2 and inhibited pASK-1, pJNK, and Bax expression as determined by both immunohistochemistry and Western blot. Caspase-3 activity and the Bax/Bcl-2 ratio declined in the HBO group. Conclusions HBO preconditioning effectively ameliorates I/R injury by regulating the apoptosis signal-regulating kinase 1 and/or c-Jun N-terminal kinase pathway and anti- and proapoptosis factors.

Published
2014

4. Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway

Author

Mingzi Zhang, Xin Zhang, You-Bin Wang, Pengxiang Zhao, Yi-Ding Xiao, Fei Xie, Yifang Liu, Xuemei Ma, Jia-La Li, Yun-Qi Liu, Wang Tingting, and Ai-Xin Cheng

Subjects
Male, medicine.medical_specialty, Necrosis, medicine.medical_treatment, H&E stain, Ischemia, Inflammation, Apoptosis, MAP Kinase Kinase Kinase 5, Free Tissue Flaps, Rats, Sprague-Dawley, medicine, Animals, Saline, Skin, bcl-2-Associated X Protein, Saline Solution, Hypertonic, TUNEL assay, business.industry, medicine.disease, Surgery, Rats, Proto-Oncogene Proteins c-bcl-2, Reperfusion Injury, medicine.symptom, business, Reperfusion injury, Hydrogen
Abstract

Summary Introduction Many pathways have been reported involving the effect of hydrogen-rich saline on protecting skin flap partial necrosis induced by the inflammation of ischemia/reperfusion injury. This study focused on the influence of hydrogen-rich saline treatment on apoptosis pathway of ASK-1/JNK and Bcl-2/Bax radio in I/R injury of skin flaps. Methods Adult male Sprague–Dawley rats were divided into three groups. Group 1 was sham surgery group, Group 2 and 3 were ischemia/reperfusion surgery treated with physiological saline and hydrogen-rich saline respectively. Blood perfusion of flap was measured by Laser doppler flowmeters. Hematoxylin and eosin staining was used to observe morphological changes. Early apoptosis in skin flap was observed through TUNEL staining and presented as the percentage of TUNEL-positive cells of total cells. pASK-1, pJNK, Bcl-2 and Bax were examined by immunodetection. In addition Bcl-2, Bax and caspase-3 were detected by qPCR. Caspase-3 activity was also measured. Results Compared to the Group 2, tissues from the group 3 were observed with a high expression of Bcl-2 and a low expression of pASK-1, pJNK, and Bax, a larger survival area and a high level of blood perfusion. Hydrogen-rich saline ameliorated inflammatory infiltration and decreased cell apoptosis. Conclusion The results indicate that hydrogen-rich saline could ameliorate ischemia/reperfusion injury and improve flap survival rate by inhibiting the apoptosis factor and, at the same time, promoting the expression of anti-apoptosis factor.

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results