Search

Your search keyword '"Telkoparan, Pelin"' showing total 2 results
Start Over Author "Telkoparan, Pelin" Topic research article
2 results on '"Telkoparan, Pelin"'

1. Coiled-Coil Domain Containing Protein 124 Is a Novel Centrosome and Midbody Protein That Interacts with the Ras-Guanine Nucleotide Exchange Factor 1B and Is Involved in Cytokinesis.

Author

Telkoparan, Pelin, Erkek, Serap, Yaman, Elif, Alotaibi, Hani, Bayık, Defne, and Tazebay, Uygar H.

Subjects
*CYTOKINESIS, *COILED bodies (Cytology), *ABSCISSION (Botany), *CENTROSOMES, *RAS proteins, *GUANINE nucleotide exchange factors, *PROTEIN-protein interactions
Abstract

Cytokinetic abscission is the cellular process leading to physical separation of two postmitotic sister cells by severing the intercellular bridge. The most noticeable structural component of the intercellular bridge is a transient organelle termed as midbody, localized at a central region marking the site of abscission. Despite its major role in completion of cytokinesis, our understanding of spatiotemporal regulation of midbody assembly is limited. Here, we report the first characterization of coiled-coil domain-containing protein-124 (Ccdc124), a eukaryotic protein conserved from fungi-to-man, which we identified as a novel centrosomal and midbody protein. Knockdown of Ccdc124 in human HeLa cells leads to accumulation of enlarged and multinucleated cells; however, centrosome maturation was not affected. We found that Ccdc124 interacts with the Ras-guanine nucleotide exchange factor 1B (RasGEF1B), establishing a functional link between cytokinesis and activation of localized Rap2 signaling at the midbody. Our data indicate that Ccdc124 is a novel factor operating both for proper progression of late cytokinetic stages in eukaryotes, and for establishment of Rap2 signaling dependent cellular functions proximal to the abscission site. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

2. The ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity

Author

Hani Alotaibi, Betül Bozkurt, Bala Gur-Dedeoglu, Mehmet Ozturk, Mine Mumcuoglu, Isik G. Yulug, Pelin Telkoparan, Uygar H. Tazebay, Burcu Cingoz, Sevgi Bagislar, Haluk Yuzugullu, K. Can Akcali, Serif Senturk, Mumcuoğlu, Mine, Bağışlar, Sevgi, Yüzügüllü, Haluk, Alotaibi, Hani, Şentürk, Şerif, Telkoparan, Pelin, Gür-Dedeoğlu, Bala, Cingöz, Burcu, Tazebay, Uygar H., Yuluğ, Işık G., Akçalı, Kamil Can, and Öztürk, Mehmet

Subjects
CD24 antigen, Pathology, medicine.medical_specialty, Cellular differentiation, Cell, Blotting, Western, Cell Biology/Cell Growth and Division, Estrogen receptor, lcsh:Medicine, Breast Neoplasms, Biology, Cyclin dependent kinase inhibitor 1, Cell Line, Tumor, Receptors, medicine, Cluster Analysis, Humans, Progenitor cell, skin and connective tissue diseases, lcsh:Science, Hermes antigen, Multidisciplinary, CD24, CD44, lcsh:R, Myoepithelial cell, Cell Biology/Cellular Death and Stress Responses, Immunohistochemistry, Tamoxifen, medicine.anatomical_structure, Receptors, Estrogen, Cell culture, Oncology/Breast Cancer, Cancer research, biology.protein, Female, lcsh:Q, Research Article
Abstract

BACKGROUND: Breast cancer is a remarkably heterogeneous disease. Luminal, basal-like, "normal-like", and ERBB2+ subgroups were identified and were shown to have different prognoses. The mechanisms underlying this heterogeneity are poorly understood. In our study, we explored the role of cellular differentiation and senescence as a potential cause of heterogeneity. METHODOLOGY/PRINCIPAL FINDINGS: A panel of breast cancer cell lines, isogenic clones, and breast tumors were used. Based on their ability to generate senescent progeny under low-density clonogenic conditions, we classified breast cancer cell lines as senescent cell progenitor (SCP) and immortal cell progenitor (ICP) subtypes. All SCP cell lines expressed estrogen receptor (ER). Loss of ER expression combined with the accumulation of p21(Cip1) correlated with senescence in these cell lines. p21(Cip1) knockdown, estrogen-mediated ER activation or ectopic ER overexpression protected cells against senescence. In contrast, tamoxifen triggered a robust senescence response. As ER expression has been linked to luminal differentiation, we compared the differentiation status of SCP and ICP cell lines using stem/progenitor, luminal, and myoepithelial markers. The SCP cells produced CD24+ or ER+ luminal-like and ASMA+ myoepithelial-like progeny, in addition to CD44+ stem/progenitor-like cells. In contrast, ICP cell lines acted as differentiation-defective stem/progenitor cells. Some ICP cell lines generated only CD44+/CD24-/ER-/ASMA- progenitor/stem-like cells, and others also produced CD24+/ER- luminal-like, but not ASMA+ myoepithelial-like cells. Furthermore, gene expression profiles clustered SCP cell lines with luminal A and "normal-like" tumors, and ICP cell lines with luminal B and basal-like tumors. The ICP cells displayed higher tumorigenicity in immunodeficient mice. CONCLUSIONS/SIGNIFICANCE: Luminal A and "normal-like" breast cancer cell lines were able to generate luminal-like and myoepithelial-like progeny undergoing senescence arrest. In contrast, luminal B/basal-like cell lines acted as stem/progenitor cells with defective differentiation capacities. Our findings suggest that the malignancy of breast tumors is directly correlated with stem/progenitor phenotypes and poor differentiation potential.

Published
2010

Catalog

Books, media, physical & digital resources
See catalog results