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Start Over Topic retroviridae infections
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1. Diagnosis of HTLV-III infection by ultrastructural examination of germinal centers in lymph node--a case report.

Author

Warner TF, Crass B, Gabel C, Maki D, Hafez GR, and Golubjatnikov R

Subjects
Acquired Immunodeficiency Syndrome pathology, Adult, Antibodies, Viral analysis, Biopsy, Collodion, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay methods, Humans, Lymph Nodes cytology, Lymph Nodes pathology, Male, Microscopy, Electron, Paper, Retroviridae Infections pathology, Retroviridae Proteins immunology, Acquired Immunodeficiency Syndrome diagnosis, Deltaretrovirus ultrastructure, Lymph Nodes ultrastructure, Retroviridae Infections diagnosis
Abstract

Serum from a patient suspected of having AIDS showed positive ELISA tests but the diagnostic p 41 band was absent and the p 24 band was barely discernible on a Western blot. Before another Western blot was performed lentivirions were detected between dendritic reticulum cells in a lymph node biopsy. It is suggested that samples of biopsies of lymph nodes from patients with or at risk for AIDS should be embedded in resin for future ultrastructural study if indicated.

Published
1986
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2. Monitoring acute phase proteins in retrovirus infected cats undergoing feline interferon‐ω therapy

Author

Rodolfo Oliveira Leal, David McGahie, Nuno Sepúlveda, Solange Gil, Luís Tavares, Maria M. R. E. Niza, and Ana Duarte

Subjects
Paper, Male, Enzyme-Linked Immunosorbent Assay, Immunodeficiency Virus, Feline, Cat Diseases, Virus, Retrovirus, Immune system, Interferon, medicine, Animals, Small Animals, Serum Amyloid A Protein, CATS, Innate immune system, biology, business.industry, Leukemia Virus, Feline, Acute-phase protein, Orosomucoid, medicine.disease, biology.organism_classification, Virology, Leukemia, C-Reactive Protein, Immunology, Papers, Interferon Type I, Leukemia, Feline, Cats, Lentivirus Infections, Female, business, medicine.drug, Retroviridae Infections
Abstract

Objectives Recombinant feline interferon-ω therapy is an immunomodulator currently used in the treatment of different retroviral diseases including feline immune deficiency virus and feline leukaemia virus. Although its mechanism of action remains unclear, this drug appears to potentiate the innate response. Acute phase proteins are one of the key components of innate immunity and studies describing their use as a monitoring tool for the immune system in animals undergoing interferon-ω therapy are lacking. This study aimed to determine whether interferon-ω therapy influences acute phase protein concentrations namely serum amyloid-A, α-1-glycoprotein and C-reactive protein. Methods A single-arm study was performed using 16 cats, living in an animal shelter, naturally infected with retroviruses and subjected to the interferon-ω therapy licensed protocol. Samples were collected before (D0), during (D10 and D30) and after therapy (D65). Serum amyloid-A and C-reactive protein were measured by specific enzyme-linked immunosorbent assay kits and α-1-glycoprotein by single radial immunodiffusion. Results All the acute phase proteins significantly increased in cats undergoing interferon-ω therapy (D0/D65: P

Published
2013

3. Diagnosis of HTLV-III infection by ultrastructural examination of germinal centers in lymph node--a case report

Author

Dennis G. Maki, Richard Golubjatnikov, Thomas F. Warner, Gholam-Reza Hafez, Barbara Crass, and Carol Gabel

Subjects
Adult, Male, Paper, Pathology, medicine.medical_specialty, Biopsy, Immunology, Lymph node biopsy, Retroviridae Proteins, Enzyme-Linked Immunosorbent Assay, Antibodies, Viral, Deltaretrovirus, Western blot, Virology, Immunopathology, medicine, Humans, Lymph node, Acquired Immunodeficiency Syndrome, medicine.diagnostic_test, business.industry, Germinal center, Collodion, Microscopy, Electron, medicine.anatomical_structure, Electrophoresis, Polyacrylamide Gel, Viral disease, Lymph, Lymph Nodes, business, Reticulum, Retroviridae Infections
Abstract

Serum from a patient suspected of having AIDS showed positive ELISA tests but the diagnostic p 41 band was absent and the p 24 band was barely discernible on a Western blot. Before another Western blot was performed lentivirions were detected between dendritic reticulum cells in a lymph node biopsy. It is suggested that samples of biopsies of lymph nodes from patients with or at risk for AIDS should be embedded in resin for future ultrastructural study if indicated.

Published
1986