1. Development of a sensor to study the DNA conformation using molecular logic gates.
- Author
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Roy AD, Dey D, Saha J, Chakraborty S, Bhattacharjee D, and Hussain SA
- Subjects
- Acriflavine pharmacology, Intercalating Agents chemistry, Intercalating Agents pharmacology, Logic, Rhodamines pharmacology, Acriflavine chemistry, Biosensing Techniques methods, DNA chemistry, Fluorescence Resonance Energy Transfer methods, Nucleic Acid Conformation drug effects, Rhodamines chemistry
- Abstract
This communication reports our investigations on the Fluorescence Resonance Energy Transfer (FRET) between two laser dyes Acriflavine and Rhodamine B in absence and presence of DNA at different pH. It has been observed that energy transfer efficiency is largely affected by the presence of DNA as well as the pH of the system. It is well known that with increase in pH, DNA conformation changes from double stranded to single stranded (denaturation) and finally form random coil. Based on our experimental results two different types of molecular logic gates namely, XOR and OR logic have been demonstrated which can be used to have an idea about DNA conformation in solution., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2015
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