1. ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells.
- Author
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Lafita-Navarro MC, Hao YH, Jiang C, Jang S, Chang TC, Brown IN, Venkateswaran N, Maurais E, Stachera W, Zhang Y, Mundy D, Han J, Tran VM, Mettlen M, Xu L, Woodruff JB, Grishin NV, Kinch L, Mendell JT, Buszczak M, and Conacci-Sorrell M
- Subjects
- Cell Nucleolus metabolism, Ribosomes metabolism, RNA, Ribosomal, 18S genetics, RNA, Ribosomal, 18S metabolism, Humans, Animals, Rats, Ribosomal Proteins metabolism, Ribosome Subunits, Small, Eukaryotic metabolism, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Transcription Factors genetics, Transcription Factors metabolism, Protein Biosynthesis
- Abstract
Increased nucleolar size and activity correlate with aberrant ribosome biogenesis and enhanced translation in cancer cells. One of the first and rate-limiting steps in translation is the interaction of the 40S small ribosome subunit with mRNAs. Here, we report the identification of the zinc finger protein 692 (ZNF692), a MYC-induced nucleolar scaffold that coordinates the final steps in the biogenesis of the small ribosome subunit. ZNF692 forms a hub containing the exosome complex and ribosome biogenesis factors specialized in the final steps of 18S rRNA processing and 40S ribosome maturation in the granular component of the nucleolus. Highly proliferative cells are more reliant on ZNF692 than normal cells; thus, we conclude that effective production of small ribosome subunits is critical for translation efficiency in cancer cells., Competing Interests: Declaration of interests The authors declare no competing interest., (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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