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The present piece of work was conducted in the Seed Physiology Laboratory of Institute of Agricultural Sciences, Banaras Hindu University in the year 2011-12, where the rice seeds variety MTU 7029 were primed with different combinations of kinetin (in ppm) and magnesium nitrate (in mM) (2.5 ppm+2 mM, 2.5 ppm+4 mM, 2.5 ppm+6 mM, 2.5 ppm+8 mM, 5 ppm+2 mM, 5 ppm+4 mM, 5 ppm+6 mM, 5 ppm+8 mM kinetin and magnesium nitrate respectively) whereas; seeds without any treatment referred as control (non-primed). Various physiomorphological (shoot and root lengths (in cm), root number, fresh and dry weights (in g)) and biochemical (proline content (mg g-1 dry weight of seedlings), total chlorophyll content (mg g-1) and superoxide dismutase activity (unit×102 g-1 min-1 fresh weight of leaf; at 20 days after sowing)) parameters were studied in the seedlings, obtained from 10, 15 and 20 DAS old primed and non-primed plants. All the primed seeds were found to perform better as compared to non primed control one. However, treatment 2.5 ppm kinetin+4 mM magnesium nitrate performed best among all the treatments and all the studied parameters such as shoot and root lengths, root number, fresh and dry weights, proline content, total chlorophyll content and superoxide dismutase activity in 10, 15 and 20 days after sowing. [ABSTRACT FROM AUTHOR]

The aim of the study is to establish a routine procedure for high frequency plant regeneration from in vitro raised embryogenic callus of indica rice var. MTU 7029. In the present piece of work, various concentrations of surfactants [sodium hypochlorite (10 to 30%) and mercuric chloride (0.01 to 1%)] were tested and best concentration of surfactants was chosen according to the parameters like callus induction percentage and its growth as well as status of contamination. On the basis of available data of the said parameters, 0.1% mercuric chloride has shown best performance in all the aspect for surface sterilization of mature rice seeds for 5 minutes and the percentage of the contamination was also very less. The effect of synthetic auxin [2,4-D (0 to 4 mg L-1)], cytokinin [kinetin (0 to 1 mg L-1)], sucrose (0 to 50 g L-1), agar (6-16 g L-1) and growth medium (MS, N6 and LS) on callus induction were optimized to achieve high frequency plant regeneration from fresh embryogenic callus without further subculture. Results suggests that 2 mg L-1 2, 4-D, 30 g L-1 sucrose, 8 g L-1 agar and MS basal medium were best for embryogenic callus induction. From the present piece of work an efficient protocol was developed for in vitro embryogenic callus formation. [ABSTRACT FROM AUTHOR]