1. A SNP-mediated lncRNA (LOC146880) and microRNA (miR-539-5p) interaction and its potential impact on the NSCLC risk.
- Author
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Feng T, Feng N, Zhu T, Li Q, Zhang Q, Wang Y, Gao M, Zhou B, Yu H, Zheng M, and Qian B
- Subjects
- Animals, Apoptosis, Biomarkers, Tumor genetics, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung metabolism, Case-Control Studies, Cell Movement, Cell Proliferation, DNA-Binding Proteins genetics, Female, Gene Expression Regulation, Neoplastic, Humans, Lung Neoplasms genetics, Lung Neoplasms metabolism, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Middle Aged, Phosphopyruvate Hydratase genetics, Prognosis, Tumor Cells, Cultured, Tumor Suppressor Proteins genetics, Xenograft Model Antitumor Assays, Biomarkers, Tumor metabolism, Carcinoma, Non-Small-Cell Lung pathology, DNA-Binding Proteins metabolism, Lung Neoplasms pathology, MicroRNAs genetics, Phosphopyruvate Hydratase metabolism, Polymorphism, Single Nucleotide, RNA, Long Noncoding genetics, Tumor Suppressor Proteins metabolism
- Abstract
Background: Many cancer-associated single nucleotide polymorphisms (SNPs) are located in the genomic regions of long non-coding RNAs (lncRNAs). Mechanisms of these SNPs in connection to cancer risk are not fully understood., Methods: Association of SNP (rs140618127) in lncRNA LOC146880 with non-small cell lung cancer (NSCLC) was evaluated in a case-control study of 2707 individuals. The mechanism of the SNP's biologic influence was explored with in vitro and in vivo experiments, including plasmid transfection, siRNA knockdown, flow cytometry assessment, and assays of cell proliferation, migration, invasion, and colony formation., Results: Association analysis showed that A allele of SNP rs140618127 was associated with low risk of NSCLC in the Chinese population. Lab experiments indicated that SNP rs140618127 contained a binding site for miR-539-5p and the binding between miR-539-5p and LOC146880 resulted in declined phosphorylation of an oncogene, ENO1. The reduced phosphorylation of ENO1 led to decreased phosphorylation of PI3K and Akt, which is further linked to the decline in cell proliferation and tumor progression., Conclusion: The study demonstrates that SNP rs140618127 in lncRNA loc146880 provides an alternate binding site for microRNA miR-539-5p which affects the phosphorylation of ENO1 and activation of the PI3K and Akt pathway.
- Published
- 2020
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