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Your search keyword '"Ghanem, Louis R."' showing total 2 results
Start Over Author "Ghanem, Louis R." Topic rna processing
2 results on '"Ghanem, Louis R."'

1. Poly(C)-Binding Protein Pcbp2 Enables Differentiation of Definitive Erythropoiesis by Directing Functional Splicing of the Runx1 Transcript.

Author

Ghanem, Louis R., Kromer, Andrew, Silverman, Ian M., Xinjun Ji, Gazzara, Matthew, Nhu Nguyen, Aguilar, Gabrielle, Martinelli, Massimo, Barash, Yoseph, and Liebhaberd, Stephen A.

Subjects
*PROTEIN binding, *REGULATION of erythropoiesis, *PHYSIOLOGICAL control systems, *GENETIC engineering, *GENETIC transcription
Abstract

Formation of the mammalian hematopoietic system is under a complex set of developmental controls. Here, we report that mouse embryos lacking the KH domain poly(C) binding protein, Pcbp2, are selectively deficient in the definitive erythroid lineage. Compared to wild-type controls, transcript splicing analysis of the Pcbp2-/- embryonic liver reveals accentuated exclusion of an exon (exon 6) that encodes a highly conserved transcriptional control segment of the hematopoietic master regulator, Runx1. Embryos rendered homozygous for a Runx1 locus lacking this cassette exon (Runx1ΔE6) effectively phenocopy the loss of the definitive erythroid lineage in Pcbp2-/- embryos. These data support a model in which enhancement of Runx1 cassette exon 6 inclusion by Pcbp2 serves a critical role in development of hematopoietic progenitors and constitutes a critical step in the developmental pathway of the definitive erythropoietic lineage. [ABSTRACT FROM AUTHOR]

Published
2018
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2. Poly(C)-Binding Protein Pcbp2 Enables Differentiation of Definitive Erythropoiesis by Directing Functional Splicing of the Runx1 Transcript

Author

Xinjun Ji, Ian M. Silverman, Stephen A. Liebhaber, Andrew Kromer, Massimo Martinelli, Yoseph Barash, Louis R. Ghanem, Matthew R. Gazzara, Gabrielle Aguilar, Nhu Nguyen, Ghanem, Louis R., Kromer, Andrew, Silverman, Ian M., Ji, Xinjun, Gazzara, Matthew, Nguyen, Nhu, Aguilar, Gabrielle, Martinelli, Massimo, Barash, Yoseph, and Liebhaber, Stephen A.

Subjects
Posttranscriptional control mechanism, 0301 basic medicine, RNA Splicing, Definitive erythropoiesi, Primitive erythropoiesi, Biology, Pcbp2, 03 medical and health sciences, Exon, chemistry.chemical_compound, Mice, Myeloid stem cell, Runx1, hemic and lymphatic diseases, Erythropoiesi, Animals, Humans, Hematopoiesi, Erythropoiesis, Amino Acid Sequence, Molecular Biology, Sequence Deletion, Phenocopy, Mice, Knockout, Base Sequence, Binding protein, Alternative splicing, Gene Expression Regulation, Developmental, RNA-Binding Proteins, Cell Differentiation, Cell Biology, Exons, KH domain, Cell biology, Globins, Hematopoiesis, 030104 developmental biology, RNA processing, RUNX1, chemistry, Liver, embryonic structures, RNA splicing, Core Binding Factor Alpha 2 Subunit, K562 Cells, Research Article
Abstract

Formation of the mammalian hematopoietic system is under a complex set of developmental controls. Here, we report that mouse embryos lacking the KH domain poly(C) binding protein, Pcbp2, are selectively deficient in the definitive erythroid lineage. Compared to wild-type controls, transcript splicing analysis of the Pcbp2-/- embryonic liver reveals accentuated exclusion of an exon (exon 6) that encodes a highly conserved transcriptional control segment of the hematopoietic master regulator, Runx1. Embryos rendered homozygous for a Runx1 locus lacking this cassette exon (Runx1ΔE6) effectively phenocopy the loss of the definitive erythroid lineage in Pcbp2-/- embryos. These data support a model in which enhancement of Runx1 cassette exon 6 inclusion by Pcbp2 serves a critical role in development of hematopoietic progenitors and constitutes a critical step in the developmental pathway of the definitive erythropoietic lineage.

Published
2018

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