1. Anti-rotavirus effects by combination therapy of stevioside and Sophora flavescens extract.
- Author
-
Alfajaro MM, Rho MC, Kim HJ, Park JG, Kim DS, Hosmillo M, Son KY, Lee JH, Park SI, Kang MI, Ryu YB, Park KH, Oh HM, Lee SW, Park SJ, Lee WS, and Cho KO
- Subjects
- Animals, Diarrhea drug therapy, Diarrhea virology, Diterpenes, Kaurane therapeutic use, Drug Therapy, Combination, Feces virology, Female, Glucosides therapeutic use, Histocytochemistry veterinary, Intestine, Small virology, Male, Plant Extracts administration & dosage, RNA, Viral chemistry, RNA, Viral genetics, Random Allocation, Real-Time Polymerase Chain Reaction veterinary, Rotavirus genetics, Rotavirus Infections drug therapy, Rotavirus Infections virology, Swine, Swine Diseases drug therapy, Diarrhea veterinary, Diterpenes, Kaurane pharmacology, Glucosides pharmacology, Plant Extracts pharmacology, Rotavirus growth & development, Rotavirus Infections veterinary, Sophora metabolism, Swine Diseases virology
- Abstract
Anti-rotaviral activities of Sophora flavescens extract (SFE) and stevioside (SV) from Stevia rebaudiana Bertoni either singly or in various combinations were examined in vitro and in vivo using a porcine rotavirus G5[P7] strain. Combination of SFE and SV inhibited in vitro virus replication more efficiently than each single treatment. In the piglet model, SV had no effect on rotavirus enteritis, whereas SFE improved but did not completely cure rotaviral enteritis. Interestingly, combination therapy of SFE and SV alleviated diarrhea, and markedly improved small intestinal lesion score and fecal virus shedding. Acute toxicity tests including the piglet lethal dose 50, and body weight, organ weight and pathological changes for the combination therapy did not show any adverse effect on the piglets. These preliminary data suggest that the combination therapy of SV and SFE is a potential curative medication for rotaviral diarrhea in pigs. Determination of the efficacy of this combination therapy in other species including humans needs to be addressed in the future., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF