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The nutritional and temperature requirements for extracellular polysaccharide (EP) production by Butyrivibrio fibrisolvens nix was determined. Strain nyx was grown anaerobically using different carbon and nitrogen sources. Results showed that EP poduction was similar at temperatures 35-40 degrees C. In addition, glucose was found to be the most efficient carbon source resulting in the fastest growth and highest EP production. The different nitrogen sources supported the same rates of growth and EP production. The resuls also showed that EP production was exponential during exponential growth but no EP was produced during the lar or stationary phases.