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The objective of this study was to evaluate the practical application of herbicide, overseeded annual ryegrass for the establishment of forage peanut (Arachis pintoi) in natural vegetation. The experiments was conducted from October 2007 to November 2008, Estação Experimental Terras Baixas (ETB) - Embrapa Clima Temperado, Capão do Leão - RS. The treatments were arranged in a randomized block design in split plots with four replications. The plots were represented by the treatments with and without herbicide (3L ha-1 of commercial herbicide glyfosate). The subplots were defined by the absence of forage peanut, the presence of forage peanut, the presence of forage peanut overseeded with annual ryegrass. The frequencies of species of natural vegetation were not influenced by grazing and overseeded ryegrass. The establishment of forage peanut Arachis pintoi is benefited by the herbicide. However, it is a practice that causes a significant reduction of species of forage interest. The presence of annual ryegrass provides a favorable environment for forage peanut during the cool season. [ABSTRACT FROM AUTHOR]