1. Antioxidant Dipeptides Enhance Osmotic Stress Tolerance by Regulating the Yeast Cell Wall and Membrane.
- Author
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Wu C, Zhang H, Yang N, Liu N, Yang H, Xu H, and Lei H
- Subjects
- Antioxidants metabolism, Osmotic Pressure, Cell Wall metabolism, Cell Membrane metabolism, Chitin Synthase metabolism, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism
- Abstract
This study aimed to investigate the role of the yeast cell wall and membrane in enhancing osmotic tolerance by antioxidant dipeptides (ADs) including Ala-His (AH), Thr-Tyr (TY), and Phe-Cys (FC). Results revealed that ADs could improve the integrity of the cell wall by restructuring polysaccharide structures. Specifically, FC significantly ( p < 0.05) reduced the leakage of nucleic acid and protein by 2.86% and 5.36%, respectively, compared to the control. In addition, membrane lipid composition played a crucial role in enhancing yeast tolerance by ADs, including the increase of cell membrane integrity and the decrease of permeability by regulating the ratio of unsaturated fatty acids. The up-regulation of gene expression associated with the cell wall integrity pathway ( RLM1 , SLT2 , MNN9 , FKS1 , and CHS3 ) and fatty acid biosynthesis ( ACC1 , HFA1 , OLE1 , ERG1 , and FAA1 ) further confirmed the positive impact of ADs on yeast tolerance against osmotic stress.
- Published
- 2024
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