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A comparative study between the performance of commercial PCR-based Salmonella enterica detection methods and the culture-based methods in spiked and naturally contaminated samples of feed mill scrapings (FMS), palm kernel meal (PKM), pelleted feed (PF), rape seed meal (RSM), soybean meal (SM), and wheat grain (WG) is presented. A statistical model that describes the detection probability when different levels (CFU) of Salmonella contamination are present in feed materials is also presented.

Sulfonamide-resistant Escherichia coli and Salmonella isolates from pigs and chickens in Ontario and Quebec are screened for sul1, sul2 and sul3 by PCR. Each sul gene is distributed differently across populations, with a significant difference between distribution in commensal Escherichia coli and Salmonella isolates and sul3 restricted mainly to porcine Escherichia coli isolates.

The plasmid component of the genome of Salmonella Kentucky CVM29188, a multidrug-resistant strain that is isolated from a chicken meat sample with resistance to streptomycin, tetracycline, ampicillin and ceftiofur, is described. A combination of in silico and in vivo approaches, including comparative plasmid transfer, and PCR-based plasmid screenings, is used for providing insights into the genetic basis for multidrug resistance phenotypes of the isolate that provide new clues about virulence evolution and host adaptation in the Salmonella serovar.