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Your search keyword '"Oronasopharyngeal swab"' showing total 2 results
Start Over Descriptor "Oronasopharyngeal swab" Topic sars-cov-2
2 results on '"Oronasopharyngeal swab"'

1. A combined oro-nasopharyngeal swab is more sensitive than mouthwash in detecting SARS-CoV-2 by a high-throughput PCR assay.

Author

Michel W, Färber J, Dilas M, Heuft HG, Tammer I, Baar J, and Kaasch AJ

Subjects
Adolescent, Adult, Asymptomatic Infections, Child, Female, Humans, Male, Middle Aged, Mouth virology, Nasopharynx virology, SARS-CoV-2 genetics, Sensitivity and Specificity, Young Adult, COVID-19 diagnosis, COVID-19 Nucleic Acid Testing, SARS-CoV-2 isolation & purification, Specimen Handling methods
Abstract

Objectives: The optimal diagnostic specimen to detect SARS-CoV-2 by PCR in the upper respiratory tract is unclear. Mouthwash fluid has been reported as an alternative to nasopharyngeal and oropharyngeal swabs. We compared mouthwash fluid with a combined oro-nasopharyngeal swab regarding test performance., Methods: In a large refugee facility, we retested individuals with a previous positive test for SARS-CoV-2 and their quarantined close contacts. All individuals were asymptomatic at the time of testing. First, a mouthwash (gargling for at least 5 s) with sterile water was performed. Then, with a single flocked swab the back of the throat and subsequently the nasopharynx were sampled. Samples were inactivated and analysed on a Roche cobas 6800 ® system with the Roche SARS-CoV-2 test., Results: Of 76 individuals, 39 (51%) tested positive for SARS-CoV-2 by oro-nasopharyngeal swab. Mouthwash detected 13 of 76 (17%) infections, but did not detect any additional infection. Samples that were positive in both tests, had lower cycle threshold (Ct)-values for oro-nasopharyngeal samples, indicating a higher virus concentration, compared to samples only positive in oro-nasopharyngeal swabs., Conclusion: Mouthwash is not as sensitive as combined oro-nasopharyngeal swab in detecting upper respiratory tract infection.

Published
2021
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2. A combined oro-nasopharyngeal swab is more sensitive than mouthwash in detecting SARS-CoV-2 by a high-throughput PCR assay

Author

Jannik Baar, Wiebke Michel, Jacqueline Färber, Achim J. Kaasch, Milica Dilas, Hans-Gert Heuft, and Ina Tammer

Subjects
0301 basic medicine, Male, Oronasopharyngeal swab, COVID19, Pcr assay, Diagnostic accuracy, 0302 clinical medicine, Sensitivity, Nasopharynx, 030212 general & internal medicine, Child, Asymptomatic Infections, Sterile water, Brief Report, General Medicine, Middle Aged, Infectious Diseases, medicine.anatomical_structure, COVID-19 Nucleic Acid Testing, Specificity, Female, medicine.symptom, Microbiology (medical), Adult, Adolescent, SARS2, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), 030106 microbiology, Asymptomatic, Sensitivity and Specificity, Specimen Handling, 03 medical and health sciences, Young Adult, stomatognathic system, Throat, medicine, Gargling, Humans, Mouthwash, 610.72, Mouth, business.industry, SARS-CoV-2, COVID-19, medicine.disease, Virology, body regions, Upper respiratory tract infection, business, Respiratory tract
Abstract

Objectives The optimal diagnostic specimen to detect SARS-CoV-2 by PCR in the upper respiratory tract is unclear. Mouthwash fluid has been reported as an alternative to nasopharyngeal and oropharyngeal swabs. We compared mouthwash fluid with a combined oro-nasopharyngeal swab regarding test performance. Methods In a large refugee facility, we retested individuals with a previous positive test for SARS-CoV-2 and their quarantined close contacts. All individuals were asymptomatic at the time of testing. First, a mouthwash (gargling for at least 5 s) with sterile water was performed. Then, with a single flocked swab the back of the throat and subsequently the nasopharynx were sampled. Samples were inactivated and analysed on a Roche cobas 6800® system with the Roche SARS-CoV-2 test. Results Of 76 individuals, 39 (51%) tested positive for SARS-CoV-2 by oro-nasopharyngeal swab. Mouthwash detected 13 of 76 (17%) infections, but did not detect any additional infection. Samples that were positive in both tests, had lower cycle threshold (Ct)-values for oro-nasopharyngeal samples, indicating a higher virus concentration, compared to samples only positive in oro-nasopharyngeal swabs. Conclusion Mouthwash is not as sensitive as combined oro-nasopharyngeal swab in detecting upper respiratory tract infection.

Published
2021

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