1. Detection of Schistosoma mansoni antibodies in a low-endemicity area using indirect immunofluorescence and circumoval precipitin test.
- Author
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Carvalho do Espírito-Santo MC, Pinto PL, Gargioni C, Alvarado-Mora MV, Pagliusi Castilho VL, Pinho JR, de Albuquerque Luna EJ, and Borges Gryschek RC
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Brazil, Child, Child, Preschool, Cross-Sectional Studies, Feces parasitology, Female, Humans, Immunoglobulin M blood, Infant, Male, Middle Aged, Schistosoma mansoni isolation & purification, Schistosomiasis mansoni parasitology, Seroepidemiologic Studies, Young Adult, Antibodies, Helminth blood, Fluorescent Antibody Technique, Indirect methods, Precipitin Tests methods, Schistosoma mansoni immunology, Schistosomiasis mansoni diagnosis
- Abstract
Parasitological diagnostic methods for schistosomiasis lack sensitivity, especially in regions of low endemicity. The objective of this study was to determine the prevalence of Schistosoma mansoni infections by antibody detection using the indirect immunofluorescence assay (IFA-IgM) and circumoval precipitin test (COPT). Serum samples of 572 individuals were randomly selected. The IFA-IgM and COPT were used to detect anti-S. mansoni antibodies. Of the patients studied, 15.9% (N = 91) were IFA-IgM positive and 5.1% (N = 29) had COPT reactions (P < 0.001 by McNemar's test). Immunodiagnostic techniques showed higher infection prevalence than had been previously estimated. This study suggests that combined use of these diagnostic tools could be useful for the diagnosis of schistosomiasis in epidemiological studies in areas of low endemicity., (© The American Society of Tropical Medicine and Hygiene.)
- Published
- 2014
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