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Mapping brain functions is crucial for neurosurgical planning in patients with drug-resistant seizures. However, presurgical language mapping using either functional or structural networks can be challenging, especially in children. In fact, most of the evidence on this topic derives from cross-sectional or retrospective studies in adults submitted to anterior temporal lobectomy. In this prospective study, we used fMRI and DTI to explore patterns of language representation, their predictors and impact on cognitive performances in 29 children and young adults (mean age at surgery: 14.6 ± 4.5 years) with focal lesional epilepsy. In 20 of them, we also assessed the influence of epilepsy surgery on language lateralization. All patients were consecutively enrolled at a single epilepsy surgery center between 2009 and 2015 and assessed with preoperative structural and functional 3T brain MRI during three language tasks: Word Generation (WG), Rhyme Generation (RG) and a comprehension task. We also acquired DTI data on arcuate fasciculus in 24 patients. We first assessed patterns of language representation (relationship of activations with the epileptogenic lesion and Laterality Index (LI)) and then hypothesized a causal model to test whether selected clinical variables would influence the patterns of language representation and the ensuing impact of the latter on cognitive performances. Twenty out of 29 patients also underwent postoperative language fMRI. We analyzed possible changes of fMRI and DTI LIs and their clinical predictors. Preoperatively, we found atypical language lateralization in four patients during WG task, in one patient during RG task and in seven patients during the comprehension task. Diffuse interictal EEG abnormalities predicted a more atypical language representation on fMRI (p = 0.012), which in turn correlated with lower attention (p = 0.036) and IQ/GDQ scores (p = 0.014). Postoperative language reorganization implied shifting towards atypical language representation. Abnormal postoperative EEG (p = 0.003) and surgical failures (p = 0.015) were associated with more atypical language lateralization, in turn correlating with worsened fluency. Neither preoperative asymmetry nor postoperative DTI LI changes in the arcuate fasciculus were observed. Focal lesional epilepsy associated with diffuse EEG abnormalities may favor atypical language lateralization and worse cognitive performances, which are potentially reversible after successful surgery.

Abstract This paper investigates trabecular bone ontogenetic changes in two different Polish populations, one prehistoric and the other historical. The studied populations are from the Brześć Kujawski region in Kujawy (north-central Poland), one from the Neolithic Period (4500–4000 BC) and one from the Middle Ages (twelfth-sixteenth centuries AD), in total 62 vertebral specimens (32 males, 30 females). Eight morphometric parameters acquired from microCT scan images were analysed. Two-way ANOVA after Box-Cox transformation and multifactorial regression model were calculated. A significant decrease in percentage bone volume fraction (BV/TV; [%]) with age at death was observed in the studied sample; Tb.N (trabecular number) was also significantly decreased with age; trabecular separation (Tb.Sp) increased with advancing age; connectivity density (Conn.D) was negatively correlated with biological age and higher in the Neolithic population. These data are found to be compatible with data from the current biomedical literature, while no loss of horizontal trabeculae was recorded as would be expected based on modern osteoporosis.

Background Empyema (the presence of pus in the pleural space) is a severe complication of community-acquired pneumonia and significant cause of morbidity, but, fortunately, not mortality in children. Between 0.6 and 2% of pneumonias are complicated by empyema and the three main pathogens involved are Streptococcus pneumoniae, Staphylococcus aureus and group A Streptococcus 1,2,3,4. Optimal management in children, especially the choice of antibiotics, method of administration and duration of therapy, pleural dranage or surgery, are still a matter of debate and currently, lack of strong specific recommendations. This paper displays the study protocol for a scoping review that aims to summarize the available literature on the microbiological epidemiology, the medical and surgical treatment options, and the outcomes of pleural empyema in pediatric population. Methods Comprehensive research combining the terms pediatric (children aged 0 to 18 years) and pleural empyema will be performed on PubMed and SCOPUS to identify all eligible studies. At first, two reviewers will screen the abstract and then their full text to determine the articles that meet the inclusion criteria. This work will be carried out independently, everyone on a different Excel spreadsheet and each researcher will be blinded to the decision of the other researcher. When the process is completed, in case of discordance, any disagreement will be identified and resolved through discussion or with help of a third author. Dissemination The findings of this review will be published in a peer-reviewed journal.

The present study investigates territorial disparities in selected socioeconomic forces and environmental factors underlying soil degradation that may lead to early desertification processes in a dry Mediterranean region exposed to increasing human pressure. To verify if spatial disparities in land resources have increased over time, a standard approach based on the Environmentally Sensitive Area Index (ESAI) was adopted to evaluate sixty years of territorial transformations in Latium, Central Italy, a region prone to intense processes of land resource depletion. The ESAI provides a standard, holistic assessment of soil degradation based on the estimation of four different ‘resource qualities’ (climate, soil, vegetation, and land use) and their change over sufficiently long time windows; in this study, the procedure was run at three reference years (1960, 1990, and 2020). The observed divergence in soil degradation levels between coastal and inland districts arose during the study period, with a consequent reduction in the local-scale variability of the ESAI. Such differential processes observed along the elevation gradient in Central Italy are likely due to anthropogenic factors affecting land use and leveraging crop intensification in flat districts and farmland abandonment in steep areas. New findings to be achieved in the context of human impacts on land resource depletion are regarded as an original contribution to the study of early desertification processes in advanced economies.

Abstract Earth’s atmosphere, whose ionization stability plays a fundamental role for the evolution and endurance of life, is exposed to the effect of cosmic explosions producing high energy Gamma-ray-bursts. Being able to abruptly increase the atmospheric ionization, they might deplete stratospheric ozone on a global scale. During the last decades, an average of more than one Gamma-ray-burst per day were recorded. Nevertheless, measurable effects on the ionosphere were rarely observed, in any case on its bottom-side (from about 60 km up to about 350 km of altitude). Here, we report evidence of an intense top-side (about 500 km) ionospheric perturbation induced by significant sudden ionospheric disturbance, and a large variation of the ionospheric electric field at 500 km, which are both correlated with the October 9, 2022 Gamma-ray-burst (GRB221009A).

Degradation and desertification represent serious threats, as they present severe environmental and socio-economic consequences, demanding immediate action. Although a recognized methodology for assessing degradation and desertification is missing, remote sensing has been recognized as a powerful support for its accessibility and efficacy. The aim of this study is to examine the application of remote sensing for assessing land and soil degradation and desertification. A total of 278 research papers retrieved from Scopus/Web of Science database and published over the past decade have been analyzed. From the analysis of scientific publications, a rising interest for these topics and a dominance of research from China has been registered. Established satellite data, Landsat, and MODIS, despite limitations in accuracy and resolution, remain popular due to easy access. This restricts research to broader scales and limits practical applications like land management. The prevalent use of vegetation indexes, while convenient, can be misleading due to their indirect connection to soil health. Consequently, vegetation-based models may not fully capture the complexities involved. To improve understanding, the study suggests a shift towards multi-indexes models and a move away from relying solely on readily available data products. Moreover, the application of data fusion methods could provide a more holistic view.

Psychiatric conditions, such as depression, anxiety, bipolar disorder, and schizophrenia, are increasingly recognized as significant risk factors for cardiovascular disease (CVD). This review systematically analyzes evidence from various databases to provide a comprehensive understanding of the impact of psychiatric illnesses on the incidence, management, and prognosis of CVD. Key findings suggest a bidirectional relationship between psychiatric disorders and CVD, indicating that mental health conditions can predispose individuals to CVD, while CVD can exacerbate or trigger psychiatric symptoms. The review explores the underlying mechanisms of these associations, including behavioral factors, stress responses, and medication side effects. It also examines the challenges in managing CVD patients with comorbid psychiatric conditions, emphasizing the importance for integrated care approaches. This review underscores the necessity of considering mental health as an integral component of cardiovascular care and calls for further research to develop tailored management strategies for these complex conditions, ultimately aiming to improve patient outcomes and quality of life. This comprehensive analysis provides valuable insights for future investigations and guides clinicians in optimizing care for patients with both psychiatric and cardiovascular conditions.

Abstract Laser-induced thermotherapy has shown promising potential for the treatment of unresectable primary pancreatic ductal adenocarcinoma tumors. Nevertheless, heterogeneous tumor environment and complex thermal interaction phenomena that are established under hyperthermic conditions can lead to under/over estimation of laser thermotherapy efficacy. Using numerical modeling, this paper presents an optimized laser setting for Nd:YAG laser delivered by a bare optical fiber (300 µm in diameter) at 1064 nm working in continuous mode within a power range of 2–10 W. For the thermal analysis, patient-specific 3D models were used, consisting of tumors in different portions of the pancreas. The optimized laser power and time for ablating the tumor completely and producing thermal toxic effects on the possible residual tumor cells beyond the tumor margins were found to be 5 W for 550 s, 7 W for 550 s, and 8 W for 550 s for the pancreatic tail, body, and head tumors, respectively. Based on the results, during the laser irradiation at the optimized doses, thermal injury was not evident either in the 15 mm lateral distances from the optical fiber or in the nearby healthy organs. The present computational-based predictions are also in line with the previous ex vivo and in vivo studies, hence, they can assist in the estimation of the therapeutic outcome of laser ablation for pancreatic neoplasms prior to clinical trials.

BackgroundThe rapid adoption of robotic surgical systems across Europe has led to a critical gap in training and credentialing for gastrointestinal (GI) surgeons. Currently, there is no existing standardised curriculum to guide robotic training, assessment and certification for GI trainees. This manuscript describes the protocol to achieve a pan-European consensus on the essential components of a comprehensive training programme for GI robotic surgery through a five-stage process.Methods and analysisIn Stage 1, a Steering Committee, consisting of international experts, trainees and educationalists, has been established to lead and coordinate the consensus development process. In Stage 2, a systematic review of existing multi-specialty robotic training curricula will be performed to inform the formulation of key position statements. In Stage 3, a comprehensive survey will be disseminated across Europe to capture the current state of robotic training and identify potential challenges and opportunities for improvement. In Stage 4, an international panel of GI surgeons, trainees, and robotic theatre staff will participate in a three-round Delphi process, seeking ≥ 70% agreement on crucial aspects of the training curriculum. Industry and patient representatives will be involved as external advisors throughout this process. In Stage 5, the robotic training curriculum for GI trainees will be finalised in a dedicated consensus meeting, culminating in the production of an Explanation and Elaboration (E&E) document.Registration detailsThe study protocol has been registered on the Open Science Framework (https://osf.io/br87d/).

Numerical prediction of material properties is attracting the attention of the scientific community and industry because of its usefulness in the design process. In the fields of fluid dynamics and microfluidics, several simulation methods have been proposed and adopted to evaluate the properties of surfaces and material interfaces, thanks to the increasing computational power available. However, despite the efforts made, a general and standardized methodology for implementing such methods is still lacking, thus requiring a trial-and-error approach for each new problem, making them difficult to implement and creating a bottleneck at the initial stage of surface design. Here, we report a validated protocol to evaluate the wettability of micro-structured surfaces with a phase-field model. Summarizing: • Simulating physical phenomena with multi-phase flows and moving contact lines can be a challenging task, due to the coupling among disparate length scales. • Using the Cahn-Hilliard diffuse-interface model, moving contact lines can be extensively investigated, although difficulties may arise when implementing numerical simulations, e.g., model parameter calibration, selection of boundary conditions, post-processing of fluid dynamics/equilibrium. • A method for employing this model and evaluating the physical consistency of the results is proposed here, considering the wettability of micro-structured surfaces as a case study.

Climatic variables are useful but often weak predictors of plant functional trait variation across ecosystems. Here the authors investigate how assigning plant communities to a habitat hierarchy improves the explanatory power of climate-trait relationships at the continental scale.

Abstract Emerging data suggests that endotheliopathy changes can be associated with post covid condition (PCC) in adults. Research on the matter in children is lacking. We analyzed an extended coagulation profile including biomarkers of endothelial damage in children with PCC and compared it with a control group of children that fully recovered post- SARS-CoV-2 infection. A case-control study enrolling children below 18 years of age with previous microbiologically confirmed SARS-CoV-2 infection in a pediatric post-covid unit in Italy ≥ 8 weeks after the initial infection. Samples were taken at 8 and 12 weeks after the SARS-CoV-2 diagnosis and analyzed for coagulation profiling (fibrinogen, prothrombin time, international normalized ratio, activated partial thromboplastin time, d-dimers, factor VIII coagulant activity, plasma von Willebrand factor (VWF) antigen and VWF ristocetin cofactor (RC)). We compared coagulation profiles in samples from children identified with PCC (at least one, or three or more symptoms, which could not be explained by an alternative diagnosis, at the 8- and 12-week follow-up assessment using the pediatric Long Covid International Severe Acute Respiratory and Emerging Infection Consortium (ISARIC) survey. Seventy-five children were enrolled, 49.3% were females, the median age was 10.2 (IQR 4.9) years. Forty-six (61%) of the children had at least one persisting symptom at the eight weeks post-onset, (PCC8); 39/75 (52%) had persistent symptoms for more than 12 weeks (PCC12) and 15/75(32%) had at least three persisting symptoms (PCC ≥ 3) at 12 weeks. Children with PCC presented more frequently with abnormal D-Dimer levels above the reference range compared to children that had fully recovered at the 8–12 weeks (39.1% vs. 17.2%, p = 0.04), and 12 week follow up or more (41% vs. 17.2%, p = 0.05), and in children with three or more symptoms at 12 weeks follow up compared to those that had recovered (64.3% vs. 22.2%, p = 0.002). For the other coagulation profiles, there were abnormal values detected for VWF, FVIII, RC and Fibrinogen but no significant differences between children with PCC compared to controls. Although the majority of children in our cohort showed coagulation profile within or close to normal ranges, we found that a higher proportion of children with PCC, and specifically those with a more severe spectrum characterized with three or more persisting symptoms, had abnormal D-dimer levels compared to other children that fully recovered from an acute SARS-CoV-2 infection.

Global patterns of regional plant diversity are relatively well known, but whether they hold for local communities is debated. This study created multi-grain global maps of alpha diversity for vascular plants to provide a nuanced understanding of plant diversity hotspots and improve predictions of global change effects on biodiversity.

Summary: The Sicilian wolf remained isolated in Sicily from the end of the Pleistocene until its extermination in the 1930s–1960s. Given its long-term isolation on the island and distinctive morphology, the genetic origin of the Sicilian wolf remains debated. We sequenced four nuclear genomes and five mitogenomes from the seven existing museum specimens to investigate the Sicilian wolf ancestry, relationships with extant and extinct wolves and dogs, and diversity. Our results show that the Sicilian wolf is most closely related to the Italian wolf but carries ancestry from a lineage related to European Eneolithic and Bronze Age dogs. The average nucleotide diversity of the Sicilian wolf was half of the Italian wolf, with 37–50% of its genome contained in runs of homozygosity. Overall, we show that, by the time it went extinct, the Sicilian wolf had high inbreeding and low-genetic diversity, consistent with a population in an insular environment.

Abstract The aim of this study was to investigate the association between uric acid (UA) and cardiometabolic risk factors (CMRFs) by sex in youth with type 1 diabetes (T1D). Retrospective data collected from 1323 children and adolescents (5–18 years; 716 boys) with T1D recruited in 9 Italian Pediatric Diabetes Centers were analyzed. CMRFs included UA, HbA1c, blood pressure (BP), cholesterol (TC), HDL, triglycerides (TG), neutrophils (N) and lymphocytes (L) count, glomerular filtration rate (eGFR) (calculated using Schwartz-Lyon equation). In boys, we found a higher age, daily insulin dose, TG, TG/HDL ratio, TC/HDL ratio, systolic BP, N/L ratio and lower HDL, and eGFR across UA tertiles (p = 0.01–0.0001). Similar results were found in girls but not for TG and systolic BP. In boys, the odds ratio (OR) of high levels of TG/HDL ratio, TC/HDL ratio, BP and mildly reduced eGFR (MRGFR) increased for 0.5 mg/dL of UA. Instead, in girls an increased levels of 0.5 mg/dL of UA were associated with high OR of TC/HDL ratio, N/L ratio and MRGFR. Uric acid may represent a useful marker for identifying youth with T1D at high cardiometabolic risk, and this association appears to vary by sex.

Recent technological advances are leading numerous researchers and professional users to the adoption of photogrammetric products for a wide range of geoscientific applications. Especially, drone-based Structure-from-Motion (SfM) photogrammetry is often applied as a high-resolution topographic modelling technique with advantages in terms of time and cost when compared to traditional surveying approaches. The aim of this work, carried out within the GeoArchaeo Sites Project, is to investigate the adaptability of drone-based surveys, even with a targetless approach, and to estimate bathymetrical accuracies in shallow waters. The approach was applied on an underwater site to show the potential for the digitalization and monitoring of an archaeological coastal geosystem in central Italy. Specifically, this work has compared the photogrammetric surveying capability of two drones including a Phantom 4 RTK (‘P4RTK’) and a low-cost Mavic Mini 2 (‘MM2’) and an Olympus TG-6 (underwater camera) for a site submerged with a maximum depth of ∼1.6 m. The assessment of the drone SfM-based products was performed through area-based and point-wise comparisons. Specifically, the area-based were assessed through an underwater photogrammetric survey obtained by acquiring images by an operator snorkeling along a portion of the site of interest. The point-wise comparison was performed using data acquired with a Global Navigation Satellite System (GNSS). This study demonstrates that coupling SfM-photogrammetry and UAS-based surveys have potential to define submerged topography. In particular, the imagery acquired with the P4RTK survey can produce dense 3D models of the underwater surface with high resolution (about 0.02 m) and bathymetric measurements with a vertical accuracy ranging between 0.06 and 0.29 m for the area-based and point-wise analysis, respectively. Thus, the approach adopted and tested involving the use of a P4RTK has the potential to reduce constraints and limitations in terms of GCPs distribution and measurement. Also, with such an approach the need for qualified operators for underwater photogrammetric workflow can be avoided.

Background: Invasive group A streptococcus infection (iGAS) is a serious, sometimes life-threatening condition, with high case fatality rates and high morbidity whose incidence is greatly increased in the last years. Despite the increasing importance and frequency of this condition, at the best of our knowledge, no previous reviews have been published focusing on the risk factors for the development of this condition and its early clinical features. This paper reports the study protocol for a scoping review that aims to analyze the early signs and clinical features of invasive group A streptococcus disease in children, to recognize the prodromal stage of the disease. Methods: Comprehensive research combining the terms pediatric and invasive group A streptococcus infection has been performed on PubMed and SCOPUS to identify potential eligible studies. The search strategy for PubMed will be available in this paper. Two reviewers will screen first the abstract and subsequently the full text to identify eligible articles according to the predefined inclusion criteria. Divergences between the reviewers will be resolved by discussion (with a third author if necessary). Two review authors will extract data independently, everyone on a different Excel spreadsheet. Each researcher will be blinded to the decision of the other researcher. When the process will be completed, in case of discordance, any disagreement will be identified and resolved through discussion (with a third author if necessary). Dissemination: The findings of this review will be published in a peer-reviewed journal.

The purpose of this study is to anatomically evaluate the impact on the patient intra vitam of an endocranial condition on a late 20th century skull housed in the Section of Legal Medicine of the University of Foggia (Foggia, Apulia, Italy). After performing a retrospective diagnosis, the condition is framed in the broader context of studies on this pathology. An anthropological and radiological analysis (X-ray and CT scan imaging) made it possible to confirm the preliminary information and to detail the osteological diagnosis of HFI. In order to assess the impact on the cerebral surface of the endocranial growth a 3D endocast was obtained using the Software OrtogOnBlender. The skull is demonstrated to have belonged to a female senile individual known, from limited documentary evidence, to have suffered from a psychiatric condition during her life. The final diagnosis is hyperostosis frontalis interna (HFI), Type D. Although a direct correlation between the demonstrated intracranial bony growth and the onset of the patient's psychiatric condition is difficult to retrospectively ascertain, the pressure exerted on this female individual's frontal lobe may have contributed to further degenerative behavioural changes in the last years of her life. This case adds to previous knowledge, especially from the palaeopathological literature, on this condition and, for the first time, presents a neuroanatomical approach to assess the global impact of the disease.

Measurement(s) Geographic Distribution Technology Type(s) digital curation Factor Type(s) country Sample Characteristic - Environment primary forest Sample Characteristic - Location Europe Machine-accessible metadata file describing the reported data: https://doi.org/10.6084/m9.figshare.14540625

SARS-CoV-2 infection of expecting mothers has been reported. Here the authors profile the peripheral blood from 14 pregnant women with asymptomatic or mild SARS-CoV-2 infection to find grossly normal immune cell composition but heterogenous induction of pro-inflammatory cytokines, thereby implicating possible therapeutic targets for virus-induced damages during pregnancy.

Abstract In recent months, Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world. COVID-19 patients show mild, moderate or severe symptoms with the latter ones requiring access to specialized intensive care. SARS-CoV-2 infections, pathogenesis and progression have not been clearly elucidated yet, thus forcing the development of many complementary approaches to identify candidate cellular pathways involved in disease progression. Host lipids play a critical role in the virus life, being the double-membrane vesicles a key factor in coronavirus replication. Moreover, lipid biogenesis pathways affect receptor-mediated virus entry at the endosomal cell surface and modulate virus propagation. In this study, targeted lipidomic analysis coupled with proinflammatory cytokines and alarmins measurement were carried out in serum of COVID-19 patients characterized by different severity degree. Serum IL-26, a cytokine involved in IL-17 pathway, TSLP and adiponectin were measured and correlated to lipid COVID-19 patient profiles. These results could be important for the classification of the COVID-19 disease and the identification of therapeutic targets.

Abstract Site conditions and forest management affect dendrometric parameters of chestnut (Castanea sativa Mill.) coppices, but there is modest knowledge on the effect of stand dendrometric characters on physical and mechanical wood characteristics. The aim of this study was to verify these relationships in chestnut coppices that were 12–14 years old. Wood density, compression and bending strength, shrinkages were measured on shoots of five different stand in a vulcanic site in Monte Amiata (Central – Italy). Investigated stands differ in number of stools/ha and dominant height, diameter/basal area of the shoots. The main difference in the physical characters among the stands is density. The initial results of the study showed that physical, mechanical wood characters are more dependent by the shoot than by the site. There is a positive relationships between the number of stools/ha and density and a negative one among shoot dominant height and basal area with wood density. Spectroscopic profile by FTIR has not showed relevant differences among the stands. Wood anatomy has showed the breakpoint at cellular level.

Lichen Planopilaris (LPP) is a lymphatic disease affecting the scalp that is characterized by a chronic and destructive inflammation process, named as ‘cicatricial alopecia’ in which the hair follicles are targeted and may involve predominantly lymphocytes or neutrophils. Scalp and biopsy layers have never been used to investigate microbial community composition and its relative taxa abundances in LPP. We sought to examine the significant taxa of this chronic relapsing inflammatory skin disease, together with inspect the existing connections with metabolic pathways featuring this microbial community. We used a multilevel analysis based on 16S rRNA marker sequencing in order to detect OTU abundances in pathologic/healthy samples, real time PCR for measuring the levels of IL-23 interleukin expression and urinary metabolomics to find out volatile organic metabolites (VOMs). By using a linear regression model, we described peculiar taxa that significantly differentiated LPP and healthy samples. We inspected taxa abundances and interleukin mRNA levels and the Microbacteriaceae family resulted negatively correlated with the IL-23 expression. Moreover, starting from 16S taxa abundances, we predicted the metabolic pathways featuring this microbial community. By inspecting microbial composition, sample richness, metabolomics profiles and the relative metabolic pathways in a cohort of LPP and healthy samples we deepened the contribution of significant taxa that are connected to inflammation maintenance and microbiota plasticity in LPP pathology.

Earthquake monitoring plays a key role in human life protection, especially in highly populated areas of the Earth. First indications have been found in SAMPEX and NOAA satellite data that particle fluxes of trapped electrons in the Van Allen belts can be correlated to seismic activity on the ground. Within the framework of the CSES mission, a systematic analysis of the electron flux, within the invariant phase space in the L-shell and equatorial pitch angle, has started with the goal to identify short-term variations of the flux (particle bursts) in conjunction with seismic activity. This analysis is based on the statistical evaluation of the flux measurements and built to be potentially implemented in an online monitoring system. The first milestone in that direction has been achieved, providing a stable background estimation. With the injection of artificial signals, the efficiency of the method was evaluated and found to be better than 95% for isolated (one per day) and short (∼3 min) signals with a significance ≥ 5σ above the background. The developed method is set up in a manner to be easily applicable to data from different instruments and satellites. This article presents the studied datasets of the low-energy HEPP instrument onboard the CSES-01 satellite and the electron telescopes of the MEPED detector onboard the NOAA POES-19 satellite, introduces the method for the background estimation, and discusses the first correlation studies of particle bursts with geomagnetic indices obtained within this framework.

Cost-effective remediation is increasingly dependent on high-resolution site characterization (HRSC), which is supposed to be necessary prior to interventions. This paper aims to evaluate the use of low-flow purging and sampling water level data in estimating the horizontal hydraulic conductivity of soils. In a new quali-quantitative view, this procedure can provide much more information and knowledge about the site, reducing time and costs. In case of high heterogeneity along the well screen, the whole procedure, as well as the estimation method, could be less effective and rigorous, with related issues in the purging time. The result showed significant permeability weighted sampling, which could provide different results as the pump position changes along the well screen. The proposed study confirms this phenomenon with field data, demonstrating that the use of multiparameter well logs might be helpful in detecting the behaviour of low-permeability layers and their effects on purging and sampling. A lower correlation between low-flow permeability estimations and LeFranc test results was associated with high heterogeneity along the screen, with a longer purging time. In wells P43, MW08 and MW36, due to the presence of clay layers, results obtained differ for almost one order of magnitude and the purging time increases (by more than 16 min). However, with some precautions prior to the field work, the low-flow purging and sampling procedure could become more representative in a shorter time and provide important hydrogeological parameters such as hydraulic conductivity with many tests and high-resolution related results.

In recent decades there has been an increasing use of remotely sensed data for precision agricultural purposes. Sericulture, the activity of rearing silkworm (Bombyx mori L.) larvae to produce silk in the form of cocoons, is an agricultural practice that has rarely used remote sensing techniques but that could benefit from them. The aim of this work was to investigate the possibility of using satellite imaging in order to monitor leaf harvesting in mulberry (Morus alba L.) plants cultivated for feeding silkworms; additionally, quantitative parameters on silk cocoon production were related to the analyses on vegetation indices. Adopting PlanetScope satellite images, four M. alba fields were monitored from the beginning of the silkworm rearing season until its end in 2020 and 2021. The results of our work showed that a decrease in the multispectral vegetation indices in the mulberry plots due to leaf harvesting was correlated with the different parameters of silk cocoons spun by silkworm larvae; in particular, a decrease in the Normalized Difference Vegetation Index (NDVI) and Soil Adjusted Vegetation Index (SAVI) had high correlations with quantitative silk cocoon production parameters (R2 values up to 0.56, p < 0.05). These results led us to the conclusion that precision agriculture can improve sericultural practice, offering interesting solutions for estimating the quantity of produced silk cocoons through the remote analysis of mulberry fields.

Since the outbreak of the Ukrainian-Russian conflict on 24 February 2022, Ukraine’s economy, society, and cities have been devastated and struck on multiple fronts, with large numbers of refugees fleeing to neighboring countries. The lighting systems in Ukrainian cities have been severely restricted due to Russian missile bombing and curfew policies. The power shortages adversely affected the livelihoods of the Ukrainian residents dramatically. For a timely assessment of the power shortages’ extent and the affected population in Ukraine, this study tracked the dynamics of nighttime light emissions in Ukraine based on the newly developed daily Black Marble product (VNP46A2) from NASA. The results show that the average light radiance in Ukrainian urban areas has decreased by about 37% since the eruption of the war, with Kiev city being the most dramatic region, having a post-conflict decrease of about 51%. In addition, by introducing near-real-time population data, we have implemented a survey of the affected population in Ukraine suffering from war-induced power shortages. Estimates show that about 17.3 million Ukrainian residents were affected by power shortages. In more detail, the number of children under 10 years old was about 2.35 million (about 5.24% of the total population), while the number of elderly people over 60 years old was about 3.53 million (about 7.86% of the total population). Generally, the results of this study could contribute positively to the timely assessment of the impact of the conflict and the implementation of humanitarian relief.

In this paper, we use the methods of networks science to analyse the transaction networks of tokens running on the Ethereum blockchain. We start with a deep dive on four of them: Ampleforth (AMP), Basic Attention Token (BAT), Dai (DAI) and Uniswap (UNI). We study two types of blockchain addresses, smart contracts (SC), which run code, and externally owned accounts (EOA), run by human users, or off-chain code, with the corresponding private keys. We use preferential attachment and network dismantling strategies to evaluate their importance for the network structure. Subsequently, we expand our view to all ERC-20 tokens issued on the Ethereum network. We first study multilayered networks composed of Ether (ETH) and individual tokens using a dismantling approach to assess how the deconstruction starting from one network affects the other. Finally, we analyse the Ether network and Ethereum-based token networks to find similarities between sets of high-degree nodes. For this purpose, we use both the traditional Jaccard Index and a new metric that we introduce, the Ordered Jaccard Index (OJI), which considers the order of the elements in the two sets that are compared. Our findings suggest that smart contracts and exchange-related addresses play a structural role in transaction networks both in DeFi and Ethereum. The presence in the network of nodes associated to addresses of smart contracts and exchanges is positively correlated with the success of the token network measured in terms of network size and market capitalisation. These nodes play a fundamental role in the centralisation of the supposedly decentralised finance (DeFi) ecosystem: without them, their networks would quickly collapse.

Traditionally, sericulture is meant as the agricultural activity of silk production, from mulberry (Morus sp.pl.) cultivation to silkworm (Bombyx mori L.) rearing. The aim of the present work is to analyze the trends and outputs of scientific research on sericulture-related topics during the last two decades, from 2000 to 2020. In this work the authors propose a text-mining analysis of the titles, abstracts and keywords of scientific articles focused on sericulture and available in the SCOPUS database considering the above-mentioned period of time; from this article collection, the 100 most recurrent terms were extracted and studied in detail. The number of publications per year in sericulture-related topics increased from 87 in 2000 to 363 in 2020 (+317%). The 100 most recurrent terms were then aggregated in clusters. The analysis shows how in the last period scientific research, besides the traditional themes of sericulture, also focused on alternative products obtainable from the sericultural practice, as fruits of mulberry trees (increment of +134% of the occurrences in the last five years) and chemical compounds as antioxidants (+233% of occurrences), phenolics (+330% of occurrences) and flavonoids (+274% of occurrences). From these considerations, the authors can state how sericulture is an active and multidisciplinary research field.

A list of 100 focal mechanism solutions that occurred in Italy between 2015 and 2019 has been compiled for earthquakes with magnitude M ≥ 4.0. We define earthquake parameters for additional 22 seismic events with 3.0 ≤ M < 4.0 for two specific key zones: Muccia, at the northern termination of the Amatrice–Visso–Norcia 2016–2018 central Italy seismic sequence, and Montecilfone (southern Italy) struck in 2018 by a deep, strike-slip Mw 5.1 earthquake apparently anomalous for the southern Apennines extensional belt. First-motion focal mechanism solutions are a good proxy for the initial rupture and they provide important additional information on the source mechanism. The catalog compiled in the present paper provides earthquake parameters for individual events of interest to contribute, as a valuable source of information, for further studies as seismotectonic investigations and stress distribution maps. We calculated the focal mechanisms using as a reference the phase pickings reported in the Italian Seismic Bulletin (BSI). We visually checked the reference picks to accurately revise manual first-motion polarities, or include new onsets when they are not present in the BSI dataset, for the selected earthquakes within the whole Italian region, with a separate focus on the Amatrice–Visso–Norcia seismic sequence area from August 24, 2016 to August 24, 2018. For the Montecilfone area, we combined the information on the geometry and kinematics of the source of the 2018 Mw 5.1 event obtained in this study with available subsurface and structural data on the Outer Apulia Carbonate Platform to improve understanding of this intriguing strike-slip sequence. Our analysis suggests that the Montecilfone earthquake ruptured a W–E trending strike-slip dextral fault. This structure is confined within the Apulia crystalline crust and it might represent the western prolongation of the Mattinata Fault–Apricena Fault active and seismogenic structures. The calculated focal mechanisms of the entire catalog are of good quality complementing important details on source mechanics from moment tensors and confirming the relevance of systematically including manually revised and more accurate polarity data within the BSI database.

In a climate-change context, the advancement of phenological stages may endanger viticultural areas in the event of a late frost. This study evaluated the potential of satellite-based remote sensing to assess the damage and the recovery time after a late frost event in 2017 in northern Italian vineyards. Several vegetation indices (VIs) normalized on a two-year dataset (2018–2019) were compared over a frost-affected area (F) and a control area (NF) using unpaired two-sample t-test. Furthermore, the must quality data (total acidity, sugar content and pH) of F and NF were analyzed. The VIs most sensitive in the detection of frost damage were Chlorophyll Absorption Ratio Index (CARI), Enhanced Vegetation Index (EVI), and Modified Triangular Vegetation Index 1 (MTVI1) (−5.26%, −16.59%, and −5.77% compared to NF, respectively). The spectral bands Near-Infrared (NIR) and Red Edge 7 were able to identify the frost damage (−16.55 and −16.67% compared to NF, respectively). Moreover, CARI, EVI, MTVI1, NIR, Red Edge 7, the Normalized Difference Vegetation Index (NDVI) and the Modified Simple Ratio (MSR) provided precise information on the full recovery time (+17.7%, +22.42%, +29.67%, +5.89%, +5.91%, +16.48%, and +8.73% compared to NF, respectively) approximately 40 days after the frost event. The must analysis showed that total acidity was higher (+5.98%), and pH was lower (−2.47%) in F compared to NF. These results suggest that medium-resolution multispectral data from Sentinel-2 constellation may represent a cost-effective tool for frost damage assessment and recovery management.

The Operational Linescan System (OLS) carried by the National Defense Meteorological Satellite Program (DMSP) can capture the weak visible radiation emitted from earth at night and produce a series of annual cloudless nighttime light (NTL) images, effectively supporting multi-scale, long-term human activities and urbanization process research. However, the interannual instability and sensor bias of NTL time series products greatly limit further studies of lighting data in time series with OLS. Several calibration models for OLS have been proposed to implement interannual corrections to improve the continuity and consistency of time series NTL products; however, due to the subjective factors intervention and insufficient automation in the calibration process, the interannual correction study of NTL time series images is still worth being developed further. Therefore, to avoid the involvement of subjective factors and to optimize the Pseudo-Invariant Features (PIF) identification, an interannual calibration model Pixel-based PIF (PBPIF) is proposed, which identifies PIF by pixel fluctuation characteristics. Results show that a PBPIF-based model can reduce subjective interference and improve the degree of automation during the NTL interannual calibration process. The calibration performance evaluation based on Total Sum of Lights (TSOL) and Sum of the Normalized Difference Index (SNDI) shows that compared to the traditional PIF-based (tPIF-based) and Ridgeline Sampling Regression based (RSR-based) models, the PBPIF-based one achieves better performance in reducing NTL interannual turbulence and minimizing the deviation between sensors. In addition, based on the corrected NTL time series products, pixel-level linear regression analysis is implemented to maximize the potential of the NTL resolution to produce global Light Intensity Change Coefficient (LICC). The results of global LICC can be widely applied to the detailed study of the characteristics of economic development and urbanization.

As an important tropospheric trace gas and precursor of photochemical smog, the accumulation of NO2 will cause serious air pollution. China, as the largest developing country in the world, has experienced a large amount of NO2 emissions in recent decades due to the rapid economic growth. Compared with the traditional air pollution monitoring technology, the rapid development of the remote sensing monitoring method of atmospheric satellite has gradually become the critical technical means of global atmospheric environmental monitoring. To reveal the NO2 pollution situation in China, based on the latest NO2 products from Sentinel-5P TROPOMI, the spatial−temporal characteristics and impact factors of troposphere NO2 column concentration of mainland China in the past year (February 2018 to January 2019) were analyzed on two administrative levels for the first time. Results show that the monthly fluctuation of tropospheric NO2 column concentration has obvious characteristics of “high in winter and low in summer”, while the spatial distribution forms a “high in East and low in west” pattern, bounded by Hu Line. The comparison of Coefficient of Variation (CV) and spatial autocorrelation models at two kinds of administrative scales indicates that although the spatial heterogeneity of NO2 column concentration is less affected by the observed scale, there is a “delayed effect” of about one month in the process of NO2 column concentration fluctuation. Besides, the impact factors analysis based on Spatial Lag Model (SLM) and Geographic Weighted Regression (GWR) reveals that there is a positive correlation between nighttime light intensity, the secondary and tertiary industries proportion and NO2 column concentration. Furthermore, for regions with serious NO2 pollution in North China Plain, the whole society electricity consumption and vehicle ownership also play a positive role in increasing the NO2 column concentration. This study will enlighten the government and policy makers to formulate policies tailored to local conditions, to more effectively implement NO2 emission reduction and air pollution prevention.

Warfarin oral anticoagulant therapy (OAT) requires regular and frequent drug adjustment monitored by INR. Interindividual variability, drug and diet interferences, and genetics (VKORC1 and CYP2C9) make the maintenance/reaching of stable INR a not so easy task. HPLC assessment of warfarin/enantiomers was suggested as a valid monitoring-tool along with INR, but definite results are still lacking. We evaluated possible correlations between INR, warfarin/3'-hydroxywarfarin, and drug weekly dosage aimed at searching novel alternatives to OAT monitoring. VKORC1/CYP2C9 pharmacogenetics investigation was performed to account for the known influence on warfarin homeostasis.133 OAT patients were recruited and assessed for warfarin/3'-hydroxywarfarin serum levels (HPLC), INR, and VKORC1 and CYP2C9 genotypes. A subgroup of 52 patients were monitored in detail (5 consecutive controls; c0-c4) till the target INR was reached. Correlation analyses were performed in both groups.In the whole OAT group both warfarin and 3'-hydroxywarfarin correlate with INR at comparable degree (r2 = 0.0388 and 0.0362 respectively). Conversely, warfarin weekly dosage better correlates with warfarin than with 3'-hydroxywarfarin (r2 = 0.0975 and r2 = 0.0381 respectively), but considering together warfarin plus 3'-hydroxywarfarin the correlation strongly increased (r2 = 0.1114; p

Everolimus is a mammalian target of rapamycin (mTOR) inhibitor approved for the treatment of metastatic renal cell carcinoma (mRCC). We aimed to assess the association between the baseline values and treatmentrelated modifications of total serum cholesterol (C), triglycerides (T), body mass index (BMI), fasting blood glucose level (FBG) and blood pressure (BP) levels and the outcome of patients treated with everolimus for mRCC.177 patients were included in this retrospective analysis. Time to progression (TTP), clinical benefit (CB) and overall survival (OS) were evaluated.Basal BMI was significantly higher in patients who experienced a CB (p=0,0145). C,T and C+T raises were significantly associated with baseline BMI (p=0.0412, 0.0283 and 0.0001). Median TTP was significantly longer in patients with T raise compared to patients without T (10 vs 6, p=0.030), C (8 vs 5, p=0.042) and C+T raise (10.9 vs 5.0, p=0.003). At the multivariate analysis, only C+T increase was associated with improved TTP (p=0.005). T raise (21.0 vs 14.0, p=0.002) and C+T increase (21.0 vs 14.0, p=0.006) were correlated with improved OS but were not significant at multivariate analysis.C+T raise is an early predictor for everolimus efficacy for patients with mRCC.

BackgroundHighly Infectious Diseases (HIDs) are (i) easily transmissible form person to person; (ii) cause a life-threatening illness with no or few treatment options; and (iii) pose a threat for both personnel and the public. Hence, even suspected HID cases should be managed in specialised facilities minimizing infection risks but allowing state-of-the-art critical care. Consensus statements on the operational management of isolation facilities have been published recently. The study presented was set up to compare the operational management, resources, and technical equipment among European isolation facilities. Due to differences in geography, population density, and national response plans it was hypothesized that adherence to recommendations will vary.Methods and findingsUntil mid of 2010 the European Network for Highly Infectious Diseases conducted a cross-sectional analysis of isolation facilities in Europe, recruiting 48 isolation facilities in 16 countries. Three checklists were disseminated, assessing 44 items and 148 specific questions. The median feedback rate for specific questions was 97.9% (n = 47/48) (range: n = 7/48 (14.6%) to n = 48/48 (100%). Although all facilities enrolled were nominated specialised facilities' serving countries or regions, their design, equipment and personnel management varied. Eighteen facilities fulfilled the definition of a High Level Isolation Unit'. In contrast, 24 facilities could not operate independently from their co-located hospital, and five could not ensure access to equipment essential for infection control. Data presented are not representative for the EU in general, as only 16/27 (59.3%) of all Member States agreed to participate. Another limitation of this study is the time elapsed between data collection and publication; e.g. in Germany one additional facility opened in the meantime.ConclusionThere are disparities both within and between European countries regarding the design and equipment of isolation facilities. With regard to the International Health Regulations, terminology, capacities and equipment should be standardised.

BackgroundBone is an uncommon site of metastasis in patients with advanced hepatocellular carcinoma (HCC). Therefore, there are few studies concerning the natural history of bone metastasis in patients with HCC.Patients and methodsData on clinicopathology, survival, skeletal-related events (SREs), and bone-directed therapies for 211 deceased HCC patients with evidence of bone metastasis were statistically analyzed.ResultsThe median age was 70 years; 172 patients were male (81.5%). The median overall survival was 19 months. The median time to the onset of bone metastasis was 13 months (22.2% at HCC diagnosis); 64.9% patients had multiple bone metastases. Spine was the most common site of bone metastasis (59.7%). Most of these lesions were osteolytic (82.4%); 88.5% of them were treated with zoledronic acid. At multivariate analysis, only the Child Score was significantly correlated with a shorter time to diagnosis of bone metastases (p = 0.001, HR = 1.819). The median survival from bone metastasis was 7 months. At multivariate analysis, HCC etiology (p = 0.005), ECOG performance status (p = 0.002) and treatment with bisphosphonate (p = 0.024) were associated with shorter survival after bone disease occurrence. The site of bone metastasis but not the number of bone lesions was associated with the survival from first skeletal related event (SRE) (p = 0.021) and OS (p = 0.001).ConclusionsThis study provides a significant improvement in the understanding the natural history of skeletal disease in HCC patients. An early and appropriate management of these patients is dramatically needed in order to avoid subsequent worsening of their quality of life.

Different assembly processes may simultaneously affect local-scale variation of species composition in temperate old-growth forests. Ground layer species diversity reflects chance colonization and persistence of low-dispersal species, as well as fine-scale environmental heterogeneity. The latter depends on both purely abiotic factors, such as soil properties and topography, and factors primarily determined by overstorey structure, such as light availability. Understanding the degree to which plant diversity in old-growth forests is associated with structural heterogeneity and/or to dispersal limitation will help assessing the effectiveness of silvicultural practices that recreate old-growth patterns and structures for the conservation or restoration of plant diversity. We used a nested sampling design to assess fine-scale species turnover, i.e. the proportion of species composition that changes among sampling units, across 11 beech-dominated old-growth forests in Southern Europe. For each stand, we also measured a wide range of environmental and structural variables that might explain ground layer species turnover. Our aim was to quantify the relative importance of dispersal limitation in comparison to that of stand structural heterogeneity while controlling for other sources of environmental heterogeneity. For this purpose, we used multiple regression on distance matrices at the within-stand extent, and mixed effect models at the extent of the whole dataset. Species turnover was best predicted by structural and environmental heterogeneity, especially by differences in light availability and in topsoil nutrient concentration and texture. Spatial distances were significant only in four out of eleven stands with a relatively low explanatory power. This suggests that structural heterogeneity is a more important driver of local-scale ground layer species turnover than dispersal limitation in southern European old-growth beech forests.

Introduction. Innate immunity is the first protection against microorganisms. Nowadays, there is a growing interest in innate immune molecule known as palate, lung, nasal epithelial clone (PLUNC). PLUNC is a specific product of the airways, of approximately 25 kDa, encoded by adjacent genes found within a 300 kb region of chromosome 20; these proteins must be detected predominantly in the upper respiratory tract. Materials and Methods. We performed a case-control study to investigate the presence of this protein in nasal tissue of patients affected by chronic rhinosinusitis. 59 patients were enrolled (44 cases, 15 controls). We have examined the correlation between the presence of pathology and the PLUNC proteins positivity. Results. 100% of controls have a +++ rated PLUNC proteins positivity, while cases have a lower percentage of positivity. We used χ2 statistical test to analyze the results of the study and there is a difference statistically significant between cases and controls in PLUNC proteins positivity. Conclusions. These observations suggest that, in response to agents or chemical factors, nasal mucosal epithelium will react and produce PLUNC proteins. So PLUNC proteins have a protective function on upper airways mucosa, as we can see by evaluating the high positivity in control group.

Promyelocytic leukemia zinc finger protein (PLZF) is a protein involved in various signaling, growth regulatory, and differentiation pathways, including development/function of some T cells. Here, we aimed at the detection of PLZF during colorectal carcinogenesis, using immunofluorescence, and at the evaluation of the colocalization of PLZF with CD2 and CD56 positive cells (T, γδ, NK, and NKT cells), using confocal-microscopy, along colorectal carcinogenesis, since its earliest stages, that is, dysplastic aberrant crypt foci (ACF). Furthermore, we analyzed PLZF in the normal colonic mucosa (NM) according to anthropometric parameters of the subject. NM exhibited strong CD56 fluorescent staining. This infiltration was lost in both ACF and colorectal carcinoma (CRC), while PLZF presence increased from NM to ACF and CRC. Strong association was found between CD56+ colonic mucosa cell infiltration and body mass index. Interestingly, an increased stromal PLZF-reactivity was present in NM of obese subjects. This study shows that overexpression of PLZF and exclusion of NK cells in dysplastic microenvironment are very early events in the stepwise sequence leading to CRC and that lower levels of CD56+ cells in NM, together with increased levels of PLZF+ cells, can be a reflection of colon cancer risk due to obesity.

We purposed to evaluate the role of Th inducing POZ-Kruppel Factor (ThPOK), a transcriptional regulator of T cell fate, in tumour-induced immune system plasticity in colorectal carcinogenesis. The amounts of CD4+, CD8+ and CD56+ and ThPOK+ cells infiltrate in normal colorectal mucosa (NM), in dysplastic aberrant crypt foci (microadenomas, MA), the earliest detectable lesions in colorectal carcinogenesis, and in colorectal carcinomas (CRC), were measured, and the colocalization of ThPOK with the above-mentioned markers of immune cells was evaluated using confocal microscopy. Interestingly, ThPOK showed a prominent increase since MA. A strong colocalization of ThPOK with CD4 both in NM and in MA was observed, weaker in carcinomas. Surprisingly, there was a peak in the colocalization levels of ThPOK with CD8 in MA, which was evident, although to a lesser extent, in carcinomas, too. In conclusion, according to the data of the present study, ThPOK may be considered a central regulator of the earliest events in the immune system during colorectal cancer development, decreasing the immune response against cancer cells.

Differently from the antiapoptotic action most commonly assigned to peroxisome proliferators (PPs), we demonstrated that some of them, clofibrate (CF) in particular, display clearcut apoptogenic properties on rat hepatoma cell lines. We and others could confirm that CF as well as various other PPs can induce apoptosis in a variety of cells, including human liver, breast and lung cancer cell lines. The present study was aimed at investigating the cytotoxic action of CF on a neoplastic line of different origin, the human T leukemia Jurkat cells. We observed that CF rapidly triggers an extensive and morphologically typical apoptotic process on Jurkat cells, though not in primary T cells, which is completely prevented by the polycaspase inhibitor zVADfmk. Gene silencing studies demonstrated that CF-induced apoptosis in Jurkat cells is partially dependent on activation of caspase 2. Looking for a possible trigger of caspase 2 activation, we observed increased levels of phosphorylated eIF2α and JNK in CF-treated cells. Moreover, intracellular Ca(2+) homeostasis was perturbed. Together, these findings are suggestive for the occurrence of ER stress, an event that is known to have the potential to activate caspase 2. The present observations demonstrate that CF induces in Jurkat cells a very fast and extensive apoptosis, that involves induction of ER stress and activation of caspases 2 and 3. Since apoptosis in Jurkat cells occurs at pharmacologically relevant concentrations of CF, the present findings encourage further in depth analysis in order to work out the potential implications of CF cytotoxcity on leukemic cells.

La lepre appenninica Lepus corsicanus nel Lazio, Italia centrale: un modello di idoneità ambientale a confronto con la distribuzione attuale. La lepre appenninica (Lepus corsicanus) è un endemismo italiano distribuito in Italia centro-meridionale, in Sicilia e in Corsica. Nel Lazio esistono piccole popolazioni, spesso isolate tra loro. Scopo di questo lavoro è la realizzazione di un modello di idoneità ambientale per questa specie. L’areale sinora conosciuto nel Lazio dimostra che il taxon è adattabile a diverse tipologie ambientali e a varie altitudini s.l.m. Il modello è stato elaborato su piattaforma GIS, attribuendo al tipo di uso del suolo (secondo la classificazione del progetto CLC) e alla quota (categorie DTM) un punteggio conforme alle preferenze ecologiche della specie. Il modello ottenuto è stato comparato con uno proposto precedentemente e confrontato con la distribuzione attuale della specie ottenuta a partire dai dati di presenza raccolti dal 1990 al 2009. Anche considerando la maggior mole di dati utilizzata nel presente studio, la differenza più evidente tra i due modelli è l’estensione maggiore delle aree ad alta idoneità ambientale da noi ottenuta. L’85.7% delle localizzazioni ricade nella classe di idoneità più elevata, suggerendo che il modello riflette le preferenze ecologiche della specie e può rappresentare un utile strumento gestionale.

Nel contesto del Progetto di riqualificazione ambientale del territorio di Capo Feto, area SIC della Provincia di Trapani si è proceduto allo studio della comunità di mammiferi dell?area. Sono state in tal modo scelte, in base alle loro caratteristiche ambientali, tre aree campione omogenee. L?attività di censimento e trappolamento si è protratta per tre diversi periodi stagionali (dal 22 al 27/10/2001, dal 19 al 24/03/2002 e dal 21 al 26/08/2002) per un totale, per ogni periodo, di 6 giorni e 5 notti trappola. Gli animali catturati sono stati registrati in apposite schede e pesati. Sono stati riportati il sesso e le condizioni riproduttive (giovane, adulto, adulto riproduttore etc.) e gli individui sono stati marcati attraverso un piccolo taglio alle orecchie, attraverso un codice di riconoscimento determinato a priori. Riguardo i micromammiferi si è effettuato un rilevamento quantitativo, attraverso la tecnica del trappolamento MCR (Cattura-marcaggio-ricattura). Sono state utilizzate 100 trappole incruente cosiddette di ?cattura e ricattura? (Ugglan modell 1, di dimensioni 250x78x65 mm). Per i mammiferi di media taglia, si è proceduto (nelle varie tipologie ambientali) con censimenti condotti di giorno e di notte (in cui tali specie mostrano maggiore attività) con l?ausilio di fari e di appositi visori per l?impiego notturno, ed attraverso rilevamenti diretti (OD: osservazione individui, esemplari rinvenuti morti) ed indiretti (OI: tracce, tane, resti ossei in borre, escrementi). Tenendo conto del basso numero di ricatture (in qualche caso uguali a zero), è stato usato l?indice di Chapman, adatto a piccoli campioni. Inoltre i dati ottenuti sono stati elaborati mediante l?indice DAT, che esprime una frequenza relativa per ogni ambiente analizzato. Per la comparazione tra i risultati (a livello di specie catturate) dei tre periodi si è utilizzato il valore medio dell?Indice di somiglianza (S) di Sorensen. I risultati dell?intero periodo di campionamento sono stati analizzati attraverso l?Indice di concentrazione di Dominanza. Complessivamente, nei tre periodi, sono stati catturati e marcati un totale di 70 micromammiferi, di cui 29 Rattus norvegicus, 17 Mus musculus, 10 R. rattus e 14 Crocidura sicula. Scorporando i risultati delle catture nei diversi periodi e per i diversi transetti, si possono evincere alcune variazioni nei popolamenti dell?area in funzione delle stagioni. Durante i diversi periodi in cui sono stati effettuati i campionamenti, infatti, l?area di Capo Feto ha mostrato differenze importanti inerenti l?umidità del suolo, che avrebbe avuto conseguenze nella composizione vegetazionale e quindi nei popolamenti dei mammiferi studiati. Sono state rilevate variazioni nei popolamenti dell?intera area, più evidenti nei riguardi di R. norvegicus e R. rattus, di minore entità riguardo il M. musculus, mentre la C. sicula si mantiene entro valori quasi costanti. I risultati totali dei trappolamenti, in cui si ha la preponderanza di R. norvegicus e M. musculus, confermerebbero il degrado dell?area in questione. Inoltre, l?area di Capo Feto sembrerebbe caratterizzata da una bassa produttività, conseguenza dell?alterazione dell?habitat, e necessita, pertanto, di immediati e profondi interventi di ripristino ambientale.

La donnola Mustela nivalis, è il più piccolo carnivoro conosciuto. È distribuita in Italia su tutta la penisola fino ai 2000 m di quota e sulle isole maggiori, e, benché sia una specie largamente diffusa anche in ambienti antropizzati, pochi sono gli studi di campo condotti in Italia, che ne mettano in luce presenza, densità, ecologia di base (dieta, uso dello spazio e dell'habitat), e genetica. La presente ricerca avviata nel gennaio 2003, prevede una raccolta di dati mensile con una presenza costante nell'area di studio, e interessa un territorio, all'interno della Riserva Regionale Parziale dei Laghi Lungo e Ripasottile, in provincia di Rieti (Lazio), caratterizzato da colture estensive e dalla presenza di una fitta rete di canali e di aree lacustri. La metodologia di studio si basa su sessioni di trappolaggio di durata variabile e a scadenza mensile. Vengono utilizzate trappole di legno incruente (in media n = 30) con esca morta (Mus musculus); gli animali catturati vengono marcati con trasponder sottocutaneo per il riconoscimento individuale, e provvisti di trasmittente radio (del peso di 1,5 g) posizionata tramite collare o zainetto. La radiolocalizzazione avviene tramite "homing", il segnale viene ricevuto a una distanza massima di 200 m, ma in media molto inferiore a causa degli ostacoli naturali e delle abitudini fossorie della donnola che diminuiscono notevolmente la capacità di ricezione del segnale. Sono stati catturati e seguiti 4 animali maschi adulti per un totale di 318 localizzazioni, 184 durante le ore diurne, 108 nelle ore notturne, e 18 durante le ore crepuscolari, prese con un intervallo di 15 minuti l'una dall'altra, e distribuite in maniera non omogenea tra gli individui. Il massimo periodo di telemetria continuativa per un animale corrisponde a 5 giorni, questo a causa della frequente perdita della radio trasmittente nelle prime 48 ore dopo il posizionamento (62,5% dei casi), perdita dovuta da un lato alla morfologia della specie e dall'altro alle abitudini fossorie. Per quello che concerne l'attività, questa ha interessato il 31,52% delle radiolocalizzazioni prese durante il giorno, il 30,77% di quelle prese al crepuscolo, e il 16,66% prese nelle ore notturne, caratterizzando uno stile di vita prevalentemente diurno, o comunque non concentrato in una particolare fase della giornata, caratteristica singolare nel panorama dei carnivori italiani. Singolare si è rivelato anche l'uso dello spazio, difatti tutte le radiolocalizzazioni, sul totale dei 4 animali, hanno interessato zone di margine e di ecotono come argini, canneti, roveti, zone di confine tra campi coltivati, canali di irrigazione, e comunque in genere zone ricche di copertura vegetale.

È stato studiato l'intero popolamento mammaliano, attraverso le catene trofiche, lungo un mega transetto che percorre oltre 1.500 Km lineari attraverso il mosaico foresta-piantagione con locale introgressione di foresta costiera di mangrovie e savana di Guinea, tra la Nigeria e il Camerun, due hot-spots per la biodiversità mammaliana come esempio di un popolamento afro-tropicale-occidentale. Sulla base delle nostre decennali investigazioni, risulta che esiste una sola specie di superpredatore di I grado (Python sebae), una di II grado (Panthera pardus), e, in particolare tra i mammiferi, una serie di predatori intermedi, come, ad es. Genetta spp., etc. Il rapporto numerico tra superpredatori e predatori tende a decrescere da est verso ovest, dai rilievi verso le pianure, e dagli ecosistemi frammentati fino a quelli omofitici, e, in sostanza, dalla foresta temporaneamente allagata alla mangrovia secondaria. Non ultima, grossa influenza viene attribuita alla distribuzione delle ingressioni salmastre che modellano la canopia e le sue "keystone species". Di particolare rilevanza ecologica risulta la debole correlazione che lega la presenza di superpredatori e predatori generalisti con specifiche prede "target", cioè a dire che la predicibilità della presenza-assenza di un determinato taxon predatore è scarsamente rilevata dalla comunità delle prede. Tutto ciò sembrerebbe logicamente derivare dall'azione delle popolazioni cacciatrici native che quindi equivarrebbero funzionalmente a dei superpredatori e predatori generalisti, che per loro stessa natura, non potrebbero essere tollerati dal carico corrente dell'ambiente residuo.