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Extracellular vesicles released in response to cigarette smoke might trigger chronic obstructive pulmonary disease, but engineered versions could be a treatment. Extracellular vesicles released in response to cigarette smoke might trigger chronic obstructive pulmonary disease, but engineered versions could be a treatment., Author(s): Jyoti Madhusoodanan Author Affiliations: The therapeutic potential of exosomes Irfan Rahman exposes cells to smoke as part of research into exosomes and COPD. [see PDF for image] Credit: Irfan [...]

The dynamics of fluid vesicles and red blood cells (RBCs) in cylindrical capillary flow is studied by using a three-dimensional mesoscopic simulation approach. As flow velocity increases, a model RBC is found to transit from a nonaxisymmetric discocyteto an axisymmetric parachute shape (coaxial with the flow axis), while a fluid vesicle is found to transit from a discocyte to a prolate ellipsoid. Both shape transitions reduce the flow resistance. The critical velocities of the shape transitions are linearly dependent on the bending rigidity and on the shear modulus of the membrane. Slipper-like shapes of the RBC model are observed around the transition velocities. Our results are in good agreement with experiments on RBCs. mesoscale hydrodynamics simulation | microfluidics | shape transformations

The study of lung emphysema dates back to the beginning of the 17th century. Nevertheless, a number of important questions remain unanswered because a quantitative localized characterization of emphysema requires knowledge of lung structure at the alveolar level in the intact living lung. This information is not available from traditional imaging modalities and pulmonary function tests. Herein, we report the first in vivo measurements of lung geometrical parameters at the alveolar level obtained with [sup.3]He diffusion MRI in healthy human subjects and patients with severe emphysema. We also provide the first experimental data demonstrating that [sup.3]He gas diffusivity in the acinus of human lung is highly anisotropic. A theory of anisotropic diffusion is presented. Our results clearly demonstrate substantial differences between healthy and emphysematous lung at the acinar level and may provide new insights into emphysema progression. The technique offers promise as a clinical tool for early diagnosis of emphysema.

Surfactant protein D (SP-D) is one of two collectins found in the pulmonary alveolus. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism. The SP-D gene was disrupted in embryonic stem cells by homologous recombination to generate mice deficient in SP-D. Mice heterozygous for the mutant SP-D allele had SP-D concentrations that were approximately 50% wild type but no other obvious phenotypic abnormality. Mice totally deficient in SP-D were healthy to 7 months but had a progressive accumulation of surfactant lipids, SP-A, and SP-B in the alveolar space. By 8 weeks the alveolar phospholipid pool was 8-fold higher than wild-type littermates. There was also a 10-fold accumulation of alveolar macrophages in the null mice, and many macrophages were both multinucleated and foamy in appearance. Type II cells in the null mice were hyperplastic and contained giant lamellar bodies. These alterations in surfactant homeostasis were not associated with detectable changes in surfactant surface activity, postnatal respiratory function, or survival. The findings in the SP-Ddeficient mice suggest a role for SP-D in surfactant homeostasis.

The cloning and characterization of the gene for non-clathrin-coat protein alpha (alpha-COP) from Saccharomyces cerevisiae reveals that it has a molecular weight of 135,500 and the presence of forty repeat motifs. The structure is similar to the bovine alpha-COP. Proteins when synthesized from the endoplasmic reticulum have to be transported to the plasma membrane. This occurs through vesicles with the involvement of a coat protein structure in mammals, made up of ADP-ribosylation factor and a coatmer, a complex of seven proteins.