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Detection of heparin (HP) under physiological conditions is difficult due to the presence of biological obstructions including proteins and lipids. Thus, it is highly challenging to selectively detect HP and to increase its sensitivity in complex systems. Here, we report the detection of HP at nanomolar levels via efficient imidazolium‐HP interaction‐assisted fluorescence quenching amplification. The self‐assembled pyrenyl aggregates are devised as a conduit for efficient exciton transport, which induces amplified fluorescence quenching for HP detection. This amplified quenching is enhanced by introducing an imidazolium receptor designed to have a high affinity to HP via electrostatic and/or additional interactions with C2 protons, resulting in a very high Stern‐Volmer quenching constant of approximately 1.17×108 M−1. [ABSTRACT FROM AUTHOR]

Keywords: self-assembly; heparin; amplified quenching; imidazolium; fluorescent sensors EN self-assembly heparin amplified quenching imidazolium fluorescent sensors 1 1 1 09/16/22 20220914 NES 220914 B A micelle-based efficient sensing approach b , exhibiting stronger amplified fluorescence quenching by introducing imidazolium capable of enhancing affinity for heparin (HP), is described. A micelle-based fluorescent sensor with these features detects HP more sensitively than conventional fluorescent sensors with aliphatic ammonium. The pyrene aggregates in the micelle provide an efficient sensing platform for amplified quenching, and the C2 proton of imidazolium on the micelle surface attracts HP very effectively, as if black holes exert pressure on their environment. [Extracted from the article]