Search

Use of carp pituitary extract (CPE) as a hormone inducer for Leporinus obtusidens is cost-prohibitive; moreover, CPE contains unknown concentrations of gonadotropins. We evaluated the efficacy of gonadorelin as a substitute by analyzing its effect on sperm characteristics of L. obtusidens and cost-effectiveness (20, 40, 60, 80, and 100μgkg -1 ). Untreated and CPE-treated (4mgkg -1 ) fish were used as negative and positive controls, respectively. Following a single intramuscular dose of hormones, semen was collected at 200°-h. At doses from 60μgkg -1 , all fish produced higher milt volume (P<0.05) than the negative control (0.2-0.8mLkg -1 ), equal (P>0.05) to the positive control (3.52±0.97mLkg -1 ). Gonadorelin did not affect sperm concentration (P>0.05). The percentage of morphologically normal spermatozoa was >64% in all treatments. The number of spermatozoa with intact membrane (70%-85%) was equal (P>0.05) to that in the controls. Relative to the negative control, gonadorelin did not affect the motility rate (P>0.05). Curvilinear velocity was equal (P>0.05) to that in both controls. The sperm swimming path was linear (0.9) 10s after activation and was not (P>0.05) dose-dependent. Considering the response rate of gonadorelin-induced fish and the similarity of semen quality compared to controls, the lowest effective dose to produce a higher volume of semen in L. obtusidens is 60μgkg -1 , and the cost to induce 1kg of L. obtusidens is 14.0% lower than that with CPE., (Copyright © 2017 Elsevier B.V. All rights reserved.)