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Bucks reproductive performance can be improved by artificial insemination technique, that affected by many factors the most important one is semen quality, which is affected by the methods of semen preservation cooling and freezing. The goal of this study was to assess the effect of adding different concentrations of antioxidant as Nano-selenium to the diluted buck semen during preservation by cooling in both summer and winter seasons. Eight mature healthy Zaraibi bucks were used in the study. Semen samples were collected by an electro- ejaculator twice per week during summer 2021 (August, September) and winter (January, February). Samples were pooled and diluted by extender then divided to experimental groups: Group 1: Nano-selenium: 100 μL /100 ml and Group 2: 200 μL / 100 ml. Then the groups cooled for measuring some semen parameters and seminal antioxidant activities after 1 h from adding and at 8 hours then every 8 hrs up to 64 hr. Nano selenium 200 μL /100 ml has a favorable impact on diluted buck semen during cooling preservation during summer and winter. [ABSTRACT FROM AUTHOR]

This work aimed to study the influences of different extender types on the quality and fertilizing capacity of stallion semen to determine the best extender which may enhance the fertilization capacity of stallion semen. Three healthy stallions aged 4-10 years and sixty mares aged 3-12 years were selected after the breeding soundness examination to accomplish this study. All the animals were with a good general health condition without any history of reproductive failures or disturbances. The external and internal genitalia were examined using the rectal palpation and ultrasonography to avoid any disturbances in the reproductive tract that might affect mare’s fertility. Semen samples were collected using artificial vagina and was evaluated, then diluted by four types of extenders (botusemen, botusemen special, botusemen gold and botusemen turbo ) and used for mares insemination to determine the influence of extender on quality of semen and conception rate.This study concluded that, the extenders types can influence the fertilizing capacity in mares through its effect on active forward motility of the sperm, dead and live sperm % and the conception rate. The extenders that contain skimmed milk could increase the fertility and conception rates of stallion semen. [ABSTRACT FROM AUTHOR]

This study was carried-out to throw the light on the effect of some biostimulants as L-carnitine; 0.1mg/ml, Omega 3; 10 ng/ml and moringa olifera; 100 μg/ml on sperm progressive motility and livability percentages, abnormalities, acrosomal integrity of rams semen cooled in refrigerator (+5) during different periods (0,24,48,72,96 hrs) in different extender [Egg yolk citrate extender 10% egg yolk (EG) (1st dil), tris based extender 2.5% EG (2nd dil), tris based extender 10% EG (3rd dil) ]. The results indicated that, the best treatment group that improved the sperm abnormalities and acrosomal integrity % observed in L-carnitine treated group followed Omega3-treated group and the least treatment observed in Moringa olifera treated group. The best treatment improved the progressive sperm motility and livability percentage of sperm cells observed in the groups treated with Omega-3, followed moringa olifera and the least observed in L-carnitine treated group, also the best diluent is tris-based extender 10% egg yolk (3rd) the results also cleared that, by increasing the time of cooling there is a decrease of sperm quality and its ability to conception. It could be concluded that, the biostimulants used in this study specially Omega-3, and moringa olifera in tris-based extender 10% egg yolk (3rd) improved semen quality of rams preserved at refrigerator for 4 days specially. [ABSTRACT FROM AUTHOR]