9 results on '"Selvaraju, Sellappan"'
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2. Deciphering the complexity of sperm transcriptome reveals genes governing functional membrane and acrosome integrities potentially influence fertility
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Selvaraju, Sellappan, Ramya, Laxman, Parthipan, Sivashanmugam, Swathi, Divakar, Binsila, Bala Krishnan, and Kolte, Atul P
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- 2021
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3. Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality
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Binsila, Bala Krishnan, Archana, Santhanahalli Siddalingappa, Ramya, Laxman, Swathi, Divakar, Selvaraju, Sellappan, Gowda, NK. Shivakumar, Pal, Din Taran, Rafay, Abu, and Bhatta, Raghavendra
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- 2021
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4. Impact of arsenic(V) on testicular oxidative stress and sperm functional attributes in Swiss albino mice
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Selvaraju Sellappan, Ravindra Janivara Parameswaraiah, and Pushpa Rani Guvvala
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Male ,0301 basic medicine ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Protein Carbonylation ,chemistry.chemical_element ,010501 environmental sciences ,Biology ,medicine.disease_cause ,01 natural sciences ,Arsenic ,Lipid peroxidation ,Mice ,03 medical and health sciences ,Semen quality ,chemistry.chemical_compound ,Internal medicine ,Testis ,medicine ,Animals ,Environmental Chemistry ,0105 earth and related environmental sciences ,media_common ,Epididymis ,Membrane potential ,Sperm Count ,Reproduction ,General Medicine ,Spermatozoa ,Pollution ,Sperm ,Semen Analysis ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,chemistry ,Sperm Motility ,Lipid Peroxidation ,Oxidative stress - Abstract
The arsenic (As) is a multi system effector including reproduction. The present study examined the association of graded doses of As(V) on testicular microenvironment and sperm function in mice. Thirty-six adult male mice were randomly assigned to six groups (n = 6). Group A served as control without test chemical. The groups B, C, D, E, and F were administered graded doses of 10, 25, 50, 100, and 200 ppm As(V), respectively, through drinking water for 40 days. A dose-dependant significant (P 0.05) decrements were observed in epididymal sperm kinematic attributes (progressive motility, rapid, fast progressive, VCL, VSL, VAP, LIN, STR, WOB and TYPE A (STR80 %, ALH 2.5 μm) by CASA), viability, plasma membrane functional integrity, and mitochondrial membrane potential which were associated with insignificant decrease in serum testosterone levels. The histoarchitectural studies of testes showed progressive loss of spermatozoa concentration in the seminiferous tubules as the As(V) dose increased. The mice exposed to As(V) had an increase in the As accumulation, protein carbonylation, and lipid peroxidation levels associated with alterations in SOD, CAT, and GST activities in the testes. In conclusion, higher doses of As(V) (more than 50 ppm) were found to be testicular toxicants which impaired semen quality by inducing oxidative stress in the testicular microenvironment.
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- 2016
5. Orchestrating the expression levels of sperm mRNAs reveals CCDC174 as an important determinant of semen quality and bull fertility.
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Selvaraju, Sellappan, Swathi, Divakar, Ramya, Laxman, Lavanya, Maharajan, Archana, Santhanahalli Siddalingappa, and Sivaram, Muniandy
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SEMEN analysis , *FERTILITY , *FROZEN semen , *SPERMATOZOA , *BULLS - Abstract
Bulls with acceptable semen quality vary in actual field fertility and this can be elucidated by studying the expression levels of mRNAs in the sperm. The present study aimed at assessing the variations in the sperm gene expression levels of PRM1, CCDC174, RPL36A, TMCO2, SWI5 and OIT3 in bulls differing in fertility status. Frozen semen samples from Holstein-Friesian bulls were classified into high-fertile (n = 8, average field conception rate = 46.1 ± 0.51, p < 0.001) and sub-fertile (n = 7, average field conception rate = 39.4 ± 0.69) groups. In the post-thaw semen samples, sperm kinematics, structural and functional membrane integrities, mitochondrial membrane potential and chromatin distribution were analyzed. The sperm total RNA was subjected to gene expression studies by Real-Time PCR. Multivariate regression analysis was performed using gene expression levels and conception rates. The sperm functional attributes did not differ significantly between the groups. The relative mRNA levels (fold change) of CCDC174 (6.20), RPL36A (4.66), SWI5 (1.86) and OIT3 (1.53) were higher in high-fertile bulls. Further, the expression level of the CCDC174 gene was significantly (p = 0.02) up-regulated in high-fertile bulls. The fertility prediction multivariate model with genes, CCDC174, RPL36A, TMCO2 and OIT3 had the maximum coefficient of determination (R2 = 0.68) with the field conception rate. This model had 93.3% bull fertility prediction accuracy with 100% sensitivity and 87.5% specificity. The study suggests that the expression level of CCDC174 can be used as a potential marker for assessing bull fertility. [ABSTRACT FROM AUTHOR]
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- 2021
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6. Current status of sperm functional genomics and its diagnostic potential of fertility in bovine (Bos taurus).
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Selvaraju, Sellappan, Parthipan, Sivashanmugam, Somashekar, Lakshminarayana, Binsila, B. Krishnan, Arangasamy, Arunachalam, Ravindra, Janivara Parameshwaraiah, Kolte, Atul P., and Krawetz, Stephen A.
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ARTIFICIAL insemination of cattle , *CATTLE , *CATTLE spermatozoa , *SPERMATOGENESIS , *EMBRYOLOGY , *CHROMATIN , *TRANSCRIPTOMES - Abstract
With artificial insemination (AI) and other precision dependent assisted reproductive technologies (ART) being followed in large scale in human and animal reproduction, assessing semen quality and fertilizability is under continuous scrutiny. Various tests have been developed to predict semen quality, but so far no single, highly reliable test is available. In this regard, transcriptomic profiling of spermatozoa assumes significance as it carries the information about spermatogenesis, sperm function, and paternal roles in post-fertilization events. Human spermatozoal transcriptome profiling has been carried out on a large number of individuals to predict the semen quality. A study in human indicated that the outcome of some idiopathic couples seeking reproductive care could be helped using transcriptomic profiling of spermatozoa. Such studies have a direct impact on the bovine dairy industry, wherein AI is practiced. Limited studies in bovine spermatozoal transcriptome profiling have revealed that the spermatozoa contain various classes of RNA, like in human. Approximately 13,000 bovine genes yield a series of spermatozoal transcripts, of which most are fragmented in nature. Their abundance is indicative of the timing of events associated with spermatogenesis, e.g., PRM1, IGF1, BMP2; sperm function, TSSK6, CRISP, HSFY2; fertility, UBE2D3, Integrin-β, LDC-1; and embryonic development, miR34c-5p, BCL2L11, BRCA1. The most abundant translated bovine transcripts are BSP3 and SPATA18, and are involved in regulation of germ cell development and the maintenance of chromatin integrity during spermatogenesis respectively. The presence of transcripts associated with placental development, e.g., placental associated glycoproteins (PAGs) have suggested their possible influence beyond early embryonic development. Changes in transcript levels like RPL31 and PRKCE that increase, and PRM1 that decreases, during cryopreservation need to be defined in order to optimize cryopreservation and fertility yield. Spermatozoal transcriptome profiling with validation studies are warranted in large numbers of animals to elucidate their significance for selecting fertile bulls for the breeding program. Abbreviations: AI: artificial insemination; BSE: breeding soundness evaluation; cfs-mRNA: cell-free seminal mRNA; piRNA: PIWI-interacting RNA; tRNA: transfer RNA; fg: femtogram; TPM: transcripts per million reads; RPKM: reads per kilobase million; rRNA: ribosomal RNA; mt-RNA: mitochondrial RNA; lncRNA: long non-coding RNA; sncRNA: small noncoding RNA; snoRNA: small nucleolar RNA; snRNA: small nuclear RNA; miRNA: microRNA; snaR: small NF90-associated RNAs; SINES: short interspersed nuclear elements; LINES: long interspersed nuclear elements; MER: medium reiterated sequence; F1 offspring: filial 1 offspring; PAGs: placental associated glycoproteins; TCP: Transcription factor T complex protein; BSP3: bovine seminal plasma protein 3; SCNT: somatic cell nuclear transfer; qPCR: quantitative (real-time) polymerase chain reaction; SSH: suppression subtractive hybridization; SNP: single nucleotide polymorphism; 2-DE: 2 dimensional gel electrophoresis; LC-MS/MS: liquid chromatography-tandem mass spectrometry [ABSTRACT FROM AUTHOR]
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- 2018
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7. Spermatozoal transcripts expression levels are predictive of semen quality and conception rate in bulls (Bos taurus).
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Parthipan, Sivashanmugam, Selvaraju, Sellappan, Somashekar, Lakshminarayana, Arangasamy, Arunachalam, Sivaram, Muniandy, and Ravindra, Janivara Parameswaraiah
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SEMEN analysis , *CATTLE , *GENE expression , *FERTILITY endocrinology , *SPERMATOZOA - Abstract
Spermatozoal transcripts expression levels could be used to assess fertility potential of a male. The objective of the present study was to elucidate the predictive ability of the expression levels of growth, apoptosis and homeostasis regulating transcripts on sperm functions and fertility. The expression levels of spermatozoal RNA isolated from the neat semen samples were related to the good (discarded ejaculate, <25%; n = 7) and poor (discarded ejaculate, >40%, n = 6) quality semen producer and bulls ( n = 12) with known conception rate. The relative fold expression levels of BMP2 were significantly ( p < 0.01) higher in good than the poor semen producers and positively associated with post-thaw sperm velocity parameters (LIN and VAP). The NGF expressions fold levels had significant ( p < 0.05) positive relationship with mitochondrial membrane potential of neat semen samples. The genes involved in the apoptotic, UBE2D3 ( r = −0.61, p = 0.02), CASP3 ( r = −0.57, p = 0.03) and homeostatic, HSFY2 ( r = −0.61, p < 0.02) regulators had significant negative correlation with the percentage of post-thaw fast progressive motile spermatozoa. The expression level of TRADD had significant negative influence on the mitochondrial membrane potential ( r = −0.54, p = 0.05) of neat semen samples and conception rate ( r = −0.57, p < 0.05). The expression levels of BMP2 had highly significant positive correlation with NGF ( r = 0.99, p < 0.01) and CASP3 ( r = 0.56, p = 0.05). The BMP2 expression level might be used to predict the quality of the semen and TRADD determine the conception rate of the bull. The study provides ample evidence that the sperm transcripts expression levels might be used to predict quality semen production and bull fertility. [ABSTRACT FROM AUTHOR]
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- 2017
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8. Relationship between seminal plasma tuberoinfundibular peptide of 39 residues and sperm functional attributes in buffalo (Bubalus bubalis).
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Selvaraju, Sellappan, Somashekar, Lakshminarayana, Krishnan, Binsila B., Parthipan, Sivashanmugam, Pushparani, Guvvala, Arangasamy, Arunachalam, Rajendran, Duraisamy, and Ravindra, Janivara Parameswaraiah
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WATER buffalo , *SEMINAL proteins , *CATTLE spermatozoa , *MITOCHONDRIAL membranes , *MEMBRANE potential - Abstract
The buffalo seminal plasma protein profile and its relationship with sperm quality have not been studied in detail. Thus, the aim of the present study was to profile buffalo seminal plasma proteins and to assess the relationship between differentially expressed proteins and sperm characteristics. Semen samples (n=44) were collected from 11 Murrah buffalo bulls (four ejaculates from each animal) and seminal plasma protein profiling was performed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Matrix-assisted laser desorption ionisation time-of-flight analysis of one of the differentially expressed proteins, namely the 11-12 kDa protein, identified it as tuberoinfundibular peptide of 39 residues (TIP39). Western blot analysis confirmed the presence of TIP39, with TIP39 expression in seminal plasma varying among bulls. Based on TIP39 levels, bulls were classified into two groups, those with high and low protein. The percentages of spermatozoa positive for mitochondrial membrane potential test, chromatin distribution test, synthetic media sperm penetrability test and acrosomal integrity test were significantly (P<0.05) high in the high protein group. The present study is the first to demonstrate the presence of TIP39 in buffalo seminal plasma and the positive effect of TIP39 on the functional parameters and fertilising ability of spermatozoa. [ABSTRACT FROM AUTHOR]
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- 2016
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9. Cryoprotective role of organic Zn and Cu supplementation in goats (Capra hircus) diet.
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Arangasamy, Arunachalam, Krishnaiah, Mayasula Venkata, Manohar, Narasimhaiah, Selvaraju, Sellappan, Rani, Guvvala Pushpa, Soren, Nira Manik, Reddy, Ippala Janardhan, and Ravindra, Janivara Parameshwaraiah
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CRYOPRESERVATION of biological cultures , *DIETARY supplements , *SEMEN analysis , *ACROSOMES , *GOATS - Abstract
The current study focused on cryopreservation and assessment of characters of post-thaw semen of indigenous Osmanabadi bucks maintained with standard diet, supplemented with different concentrations of organic zinc (Zn), copper (Cu) or in combination, for a period of 180 days. The different doses of organic Zn and Cu were fed per kg DM basis, Zn groups (low: Zn20, medium: Zn40 and high: Zn60), Cu groups: (low: Cu12.5, medium: Cu25 and high: Cu37.5) and combination of Zn + Cu groups (low: Zn20 + Cu12.5, medium: Zn40 + Cu25 and high: Zn60 + Cu37.5) respectively. The control group bucks were maintained mainly on the basal diet without any additional mineral supplementation. Two hundred and forty (240) semen samples were collected from 40 bucks aged 11 months, through electro ejaculator method, processed and analysed for sperm quality parameters both at pre freeze and post-thaw stage. The semen samples were diluted in Tris egg yolk extender, cooled and equilibrated for 4 h at 5 °C, cryopreserved using programmable freezer (PLANER Kryo 360–1.7) and stored at −196 °C. The organic trace minerals (Zn, Cu and Zn + Cu) protected the spermatozoa against the cryoinjury and maintained higher post-thaw semen parameters except in high Zn group. Additional feeding of organic Cu and Zn to bucks had a protective role and resulted in higher sperm liveability, plasma membrane and acrosome integrities, motility and velocity and reduced oxidative stress in supplemented goats (P < 0.05). [ABSTRACT FROM AUTHOR]
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- 2018
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