41 results on '"Whisstock, James C"'
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2. High resolution structure of cleaved Serpin 42 Da from Drosophila melanogaster.
- Author
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Ellisdon AM, Zhang Q, Henstridge MA, Johnson TK, Warr CG, Law RH, and Whisstock JC
- Subjects
- Alternative Splicing, Amino Acid Sequence, Animals, Crystallography, X-Ray, Drosophila Proteins metabolism, Models, Molecular, Molecular Sequence Data, Protein Conformation, Protein Isoforms chemistry, Protein Isoforms metabolism, Protein Structure, Secondary, Protein Structure, Tertiary, Sequence Alignment, Serine Proteinase Inhibitors metabolism, Serpins metabolism, Drosophila Proteins chemistry, Drosophila melanogaster metabolism, Serine Proteinase Inhibitors chemistry, Serpins chemistry
- Abstract
Background: The Drosophila melanogaster Serpin 42 Da gene (previously Serpin 4) encodes a serine protease inhibitor that is capable of remarkable functional diversity through the alternative splicing of four different reactive centre loop exons. Eight protein isoforms of Serpin 42 Da have been identified to date, targeting the protease inhibitor to both different proteases and cellular locations. Biochemical and genetic studies suggest that Serpin 42 Da inhibits target proteases through the classical serpin 'suicide' inhibition mechanism, however the crystal structure of a representative Serpin 42 Da isoform remains to be determined., Results: We report two high-resolution crystal structures of Serpin 42 Da representing the A/B isoforms in the cleaved conformation, belonging to two different space-groups and diffracting to 1.7 Å and 1.8 Å. Structural analysis reveals the archetypal serpin fold, with the major elements of secondary structure displaying significant homology to the vertebrate serpin, neuroserpin. Key residues known to have central roles in the serpin inhibitory mechanism are conserved in both the hinge and shutter regions of Serpin 42 Da. Furthermore, these structures identify important conserved interactions that appear to be of crucial importance in allowing the Serpin 42 Da fold to act as a versatile template for multiple reactive centre loops that have different sequences and protease specificities., Conclusions: In combination with previous biochemical and genetic studies, these structures confirm for the first time that the Serpin 42 Da isoforms are typical inhibitory serpin family members with the conserved serpin fold and inhibitory mechanism. Additionally, these data reveal the remarkable structural plasticity of serpins, whereby the basic fold is harnessed as a template for inhibition of a large spectrum of proteases by reactive centre loop exon 'switching'. This is the first structure of a Drosophila serpin reported to date, and will provide a platform for future mutational studies in Drosophila to ascertain the functional role of each of the Serpin 42 Da isoforms.
- Published
- 2014
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3. Maspin is not required for embryonic development or tumour suppression.
- Author
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Teoh SS, Vieusseux J, Prakash M, Berkowicz S, Luu J, Bird CH, Law RH, Rosado C, Price JT, Whisstock JC, and Bird PI
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- Animals, Cell Line, Tumor, Humans, Mice, Mice, Knockout, Embryonic Development physiology, Neoplasms physiopathology, Serpins physiology
- Abstract
Maspin (SERPINB5) is accepted as an important tumour suppressor lost in many cancers. Consistent with a critical role in development or differentiation maspin knockout mice die during early embryogenesis, yet clinical data conflict on the prognostic utility of maspin expression. Here to reconcile these findings we made conditional knockout mice. Surprisingly, maspin knockout embryos develop into overtly normal animals. Contrary to original reports, maspin re-expression does not inhibit tumour growth or metastasis in vivo, or influence cell migration, invasion or survival in vitro. Bioinformatic analyses reveal that maspin is not commonly under-expressed in cancer, and that perturbation of genes near maspin may in fact explain poor survival in certain patient cohorts with low maspin expression.
- Published
- 2014
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4. A versatile monoclonal antibody specific to human SERPINB5.
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Teoh SS, Wang H, Risbridger GP, Whisstock JC, and Bird PI
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- Animals, Antibodies, Monoclonal biosynthesis, Blotting, Western, COS Cells, Cell Line, Tumor, Chlorocebus aethiops, Enzyme-Linked Immunosorbent Assay, Escherichia coli genetics, Gene Expression, Humans, Mice, Protein Binding, Protein Conformation, Protein Denaturation, Recombinant Proteins analysis, Recombinant Proteins genetics, Recombinant Proteins immunology, Serpins genetics, Serpins immunology, Transfection, Antibodies, Monoclonal immunology, Antibody Specificity, Serpins analysis
- Abstract
Maspin (SERPINB5) is a member of the Clade B subgroup of the large superfamily of serine protease inhibitors. It is proposed that maspin is a tumor suppressor; however, its molecular role remains to be elucidated. Here we report the characterization of a mouse monoclonal antibody directed against human maspin. This antibody, 16F7, recognizes maspin in both its native and denatured form, unlike several other commercial antibodies tested in this study. It will be a useful and versatile tool for future analyses of the biological function of maspin.
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- 2012
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5. Serpin structure and evolution. Preface.
- Author
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Bird PI and Whisstock JC
- Subjects
- Animals, Humans, Protease Inhibitors metabolism, Protein Conformation, Protein Folding, Proteostasis Deficiencies genetics, Proteostasis Deficiencies metabolism, Serpins genetics, Serpins metabolism, Biological Evolution, Protease Inhibitors chemistry, Serpins chemistry
- Published
- 2011
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6. Predicting serpin/protease interactions.
- Author
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Song J, Matthews AY, Reboul CF, Kaiserman D, Pike RN, Bird PI, and Whisstock JC
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- Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Humans, Models, Molecular, Molecular Sequence Data, Protein Binding drug effects, Protein Interaction Domains and Motifs drug effects, Protein Structure, Secondary drug effects, Proteome chemistry, Proteome genetics, Serpins chemistry, Serpins pharmacology, Substrate Specificity, Computational Biology methods, Granzymes metabolism, Peptide Library, Proteolysis drug effects, Proteome metabolism, Serpins metabolism
- Abstract
Proteases are tightly regulated by specific inhibitors, such as serpins, which are able to undergo considerable and irreversible conformational changes in order to trap their targets. There has been a considerable effort to investigate serpin structure and functions in the past few decades; however, the specific interactions between proteases and serpins remain elusive. In this chapter, we describe detailed experimental protocols to determine and characterize the extended substrate specificity of proteases based on a substrate phage display technique. We also describe how to employ a bioinformatics system to analyze the substrate specificity data obtained from this technique and predict the potential inhibitory serpin partners of a protease (in this case, the immune protease, granzyme B) in a step-by-step manner. The method described here could also be applied to other proteases for more generalized substrate specificity analysis and substrate discovery., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
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7. Biology of serpins. Preface.
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Whisstock JC and Bird PI
- Subjects
- Humans, Serpins chemistry, Serpins metabolism
- Published
- 2011
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8. Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions.
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Whisstock JC, Silverman GA, Bird PI, Bottomley SP, Kaiserman D, Luke CJ, Pak SC, Reichhart JM, and Huntington JA
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- Animals, Biological Transport, Biophysics methods, Catalytic Domain, Hormones chemistry, Humans, Kinetics, Models, Biological, Protein Binding, Protein Conformation, Protein Structure, Tertiary, Serpins chemistry, Substrate Specificity, Thrombin chemistry, Peptide Hydrolases chemistry, Serpins physiology
- Abstract
Inhibitory serpins are metastable proteins that undergo a substantial conformational rearrangement to covalently trap target peptidases. The serpin reactive center loop contributes a majority of the interactions that serpins make during the initial binding to target peptidases. However, structural studies on serpin-peptidase complexes reveal a broader set of contacts on the scaffold of inhibitory serpins that have substantial influence on guiding peptidase recognition. Structural and biophysical studies also reveal how aberrant serpin folding can lead to the formation of domain-swapped serpin multimers rather than the monomeric metastable state. Serpin domain swapping may therefore underlie the polymerization events characteristic of the serpinopathies. Finally, recent structural studies reveal how the serpin fold has been adapted for non-inhibitory functions such as hormone binding.
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- 2010
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9. Serpins flex their muscle: I. Putting the clamps on proteolysis in diverse biological systems.
- Author
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Silverman GA, Whisstock JC, Bottomley SP, Huntington JA, Kaiserman D, Luke CJ, Pak SC, Reichhart JM, and Bird PI
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- Animals, Caenorhabditis elegans, Cell Death, Cell Differentiation, Cell Survival, Drosophila melanogaster, Homeostasis, Humans, Immunity, Innate, Mice, Mice, Transgenic, Models, Biological, Phenotype, Serpins chemistry, Transgenes, Serpins physiology
- Abstract
Serpins compose the largest superfamily of peptidase inhibitors and are well known as regulators of hemostasis and thrombolysis. Studies using model organisms, from plants to vertebrates, now show that serpins and their unique inhibitory mechanism and conformational flexibility are exploited to control proteolysis in molecular pathways associated with cell survival, development, and host defense. In addition, an increasing number of non-inhibitory serpins are emerging as important elements within a diversity of biological systems by serving as chaperones, hormone transporters, or anti-angiogenic factors.
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- 2010
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10. Molecular contortionism - on the physical limits of serpin 'loop-sheet' polymers.
- Author
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Huntington JA and Whisstock JC
- Subjects
- Computer Simulation, Databases, Protein, Humans, Models, Molecular, alpha 1-Antitrypsin chemistry, Protein Folding, Protein Interaction Domains and Motifs, Protein Multimerization, Protein Structure, Quaternary, Serine Proteinase Inhibitors chemistry, Serpins chemistry
- Abstract
Members of the serpin (serine protease inhibitor) superfamily fold into a metastable conformation that is crucial for proper function. As a consequence, serpins are susceptible to mutations that cause misfolding and the intracellular accumulation of pathogenic polymers. The mechanism of serpin polymerisation remains to be resolved, however, over the past two decades the 'loop-sheet' hypothesis has gained wide acceptance. In this mechanism the reactive centre loop of one serpin monomer inserts into the beta-sheet A of another (in trans), in a manner similar to what is seen for reactive centre loop-cleaved and latent conformations (in cis). The hypothesis has been refined in response to certain experimental data, but it has proved difficult to assess the various propositions without creating molecular models. Here we evaluate the loop-sheet mechanism by creating models of pentamers of the archetypal serpin alpha(1)-antitrypsin. We conclude that an inescapable consequence of the loop-sheet mechanism is polymer compaction and rigidity, properties that are inconsistent with the 'beads-on-a-string' morphology of polymers obtained from human tissue. The recent crystal structure of a domain-swapped serpin dimer suggests an alternative mechanism that is consistent with known polymer properties, including the requirement of partial unfolding to induce polymer formation in vitro, and polymerisation from a folding intermediate in vivo.
- Published
- 2010
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11. Maspin (SERPINB5) is an obligate intracellular serpin.
- Author
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Teoh SS, Whisstock JC, and Bird PI
- Subjects
- Animals, Biotinylation, Breast cytology, COS Cells, Cell Differentiation, Cell Membrane metabolism, Cells, Cultured, Chlorocebus aethiops, Female, Fluorescent Antibody Technique, Indirect, Humans, Male, Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase metabolism, Peptide Fragments metabolism, Prostate cytology, Serine Proteinase Inhibitors genetics, Serpins genetics, Breast metabolism, Cell Nucleus metabolism, Cytoplasm metabolism, Prostate metabolism, Serine Proteinase Inhibitors metabolism, Serpins metabolism
- Abstract
Maspin (SERPINB5) is a tumor suppressor lost in breast and prostate cancer whose molecular function is unknown. It is a non-inhibitory member of the clade B serpins suggested to play a role in a plethora of intracellular and extracellular settings, yet its normal cellular distribution has never been clarified. Here we investigate the distribution of maspin in non-transformed human epithelial cells. By indirect immunofluorescence, maspin has a nucleocytoplasmic distribution in breast (MCF10A) and prostate (RWPE-1) cells and, by immunoblotting and pulse-chase analyses, is neither glycosylated nor secreted. Cell surface biotinylation studies also show that maspin is not present at the cell surface. Differentiation of MCF10A cells into three-dimensional acini results in the redistribution of maspin from the nucleus to the cytoplasm but does not result in secretion. Addition of an efficient conventional signal peptide to maspin directs it into the secretory pathway and results in glycosylation but not secretion. We further show that maspin in the cytoplasm of MCF10A cells is a soluble monomeric protein that is not detectably associated with the cytoskeleton or other extractable components. Taken together, these results suggest that maspin is restricted to an intracellular, possibly nuclear, role in which it influences cell-matrix interactions indirectly. It is probably released only as a consequence of cell damage or necrosis.
- Published
- 2010
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12. A serpin in the cellulosome of the anaerobic fungus Piromyces sp. strain E2.
- Author
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Steenbakkers PJ, Irving JA, Harhangi HR, Swinkels WJ, Akhmanova A, Dijkerman R, Jetten MS, van der Drift C, Whisstock JC, and Op den Camp HJ
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- Amino Acid Sequence, Anaerobiosis, Base Sequence, Cellulosomes chemistry, Cellulosomes metabolism, Conserved Sequence, Fungal Proteins chemistry, Fungal Proteins metabolism, Molecular Sequence Data, Piromyces chemistry, Piromyces metabolism, Sequence Alignment, Serpins chemistry, Serpins metabolism, Cellulosomes genetics, Fungal Proteins genetics, Piromyces genetics, Serpins genetics
- Abstract
A gene encoding a novel component of the cellulolytic complex (cellulosome) of the anaerobic fungus Piromyces sp. strain E2 was identified. The encoded 538 amino acid protein, named celpin, consists of a signal peptide, a positively charged domain of unknown function followed by two fungal dockerins, typical for components of the extracellular fungal cellulosome. The C-terminal end consists of a 380 amino acid serine proteinase inhibitor (or serpin) domain homologue, sharing 30% identity and 50% similarity to vertebrate and bacterial serpins. Detailed protein sequence analysis of the serpin domain revealed that it contained all features of a functional serpin. It possesses the conserved amino acids present in more than 70% of known serpins, and it contained the consensus of inhibiting serpins. Because of the confined space of the fungal cellulosome inside plant tissue and the auto-proteolysis of plant material in the rumen, the fungal serpin is presumably involved in protection of the cellulosome against plant proteinases. The celpin protein of Piromyces sp. strain E2 is the first non-structural, non-hydrolytic fungal cellulosome component. Furthermore, the celpin protein of Piromyces sp. strain E2 is the first representative of a serine proteinase inhibitor of the fungal kingdom.
- Published
- 2008
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13. A structural basis for loop C-sheet polymerization in serpins.
- Author
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Zhang Q, Law RH, Bottomley SP, Whisstock JC, and Buckle AM
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- Bacterial Proteins genetics, Bacterial Proteins metabolism, Crystallography, X-Ray, Gene Deletion, Humans, Models, Molecular, Protein Conformation, Protein Folding, Serpins genetics, Serpins metabolism, Bacterial Proteins chemistry, Serpins chemistry, Thermoanaerobacter chemistry
- Abstract
In this study, we report the X-ray crystal structure of an N-terminally truncated variant of the bacterial serpin, tengpin (tengpinDelta42). Our data reveal that tengpinDelta42 adopts a variation of the latent conformation in which the reactive center loop is hyperinserted into the A beta-sheet and removed from the vicinity of the C-sheet. This conformational change leaves the C beta-sheet completely exposed and permits antiparallel edge-strand interactions between the exposed portion of the reactive center loop of one molecule and strand s2C of the C beta-sheet of the neighboring molecule in the crystal lattice. Our structural data thus reveal that tengpinDelta42 forms a loop C-sheet polymer in the crystal lattice. In vivo serpins have a propensity to misfold and form long-chain polymers, a process that underlies serpinopathies such as emphysema, thrombosis and dementia. Native serpins are thought to polymerize via a loop A-sheet mechanism. However, studies on plasminogen activator inhibitor 1 and the S49P variant of human neuroserpin reveal that the latent form of these molecules can also polymerize. Polymerization of latent neuroserpin may be important for the development of familial encephalopathy with neuroserpin inclusion bodies. Our structural data provide a possible mechanism for polymerization by latent serpins.
- Published
- 2008
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14. DNA accelerates the inhibition of human cathepsin V by serpins.
- Author
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Ong PC, McGowan S, Pearce MC, Irving JA, Kan WT, Grigoryev SA, Turk B, Silverman GA, Brix K, Bottomley SP, Whisstock JC, and Pike RN
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- Antigens, Neoplasm metabolism, Cathepsin L, Cathepsins metabolism, Coenzymes metabolism, Cystatin A, Cystatins antagonists & inhibitors, Cystatins chemistry, Cystatins metabolism, Cysteine Endopeptidases metabolism, Humans, Protein Binding physiology, Protein Conformation, Serpins metabolism, Antigens, Neoplasm chemistry, Cathepsins antagonists & inhibitors, Cathepsins chemistry, Coenzymes chemistry, Cysteine Endopeptidases chemistry, DNA chemistry, Serpins chemistry
- Abstract
A balance between proteolytic activity and protease inhibition is crucial to the appropriate function of many biological processes. There is mounting evidence for the presence of both papain-like cysteine proteases and serpins with a corresponding inhibitory activity in the nucleus. Well characterized examples of cofactors fine tuning serpin activity in the extracellular milieu are known, but such modulation has not been studied for protease-serpin interactions within the cell. Accordingly, we present an investigation into the effect of a DNA-rich environment on the interaction between model serpins (MENT and SCCA-1), cysteine proteases (human cathepsin V and human cathepsin L), and cystatin A. DNA was indeed found to accelerate the rate at which MENT inhibited cathepsin V, a human orthologue of mammalian cathepsin L, up to 50-fold, but unexpectedly this effect was primarily effected via the protease and secondarily by the recruitment of the DNA as a "template" onto which cathepsin V and MENT are bound. Notably, the protease-mediated effect was found to correspond both with an altered substrate turnover and a conformational change within the protease. Consistent with this, cystatin inhibition, which relies on occlusion of the active site rather than the substrate-like behavior of serpins, was unaltered by DNA. This represents the first example of modulation of serpin inhibition of cysteine proteases by a co-factor and reveals a mechanism for differential regulation of cathepsin proteolytic activity in a DNA-rich environment.
- Published
- 2007
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15. SerpinB6 is an inhibitor of kallikrein-8 in keratinocytes.
- Author
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Scott FL, Sun J, Whisstock JC, Kato K, and Bird PI
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- Animals, Cells, Cultured, Humans, Intracellular Fluid enzymology, Intracellular Fluid metabolism, Kallikreins metabolism, Keratinocytes cytology, Keratinocytes metabolism, Mice, Mice, Inbred C57BL, Serpins chemistry, Kallikreins antagonists & inhibitors, Keratinocytes enzymology, Serpins physiology
- Abstract
SerpinB6 (Proteinase inhibitor 6/PI-6) is an intracellular serpin produced by leukocytes, platelets, endothelial cells, keratinocytes and other epithelial cells. It is a potent cathepsin G inhibitor thought to protect monocytes, neutrophils and bystander cells from ectopic cathepsin G during inflammation. Here we show that serpinB6 also inhibits the human serine protease kallikrein-8 (hK8) and that in human and mouse skin, serpinB6 and kallikrein-8 co-localize in differentiated keratinocytes. SerpinB6 inhibits hK8 with an association rate constant (kass) of 1.8 +/- 0.2 x 10(5) M(-1)s(-1) compared to 3.4 +/- 0.2 x 10(6) M(-1) s(-1) for the interaction between the mouse orthologue of serpinB6 (SPI3/serpinb6a) and mouse kallikrein-8 (mK8). Molecular modelling suggested that the lower efficiency of the serpinB6/hK8 interaction is partly due to the bulkier P2 methionine residue of serpinB6 compared to the smaller P2 valine in SPI3. Taken together, these results suggest that serpinB6 is a physiologically relevant inhibitor of hK8 in skin. We postulate that serpinB6 protects the intracellular compartment of keratinocytes from ectopic hK8.
- Published
- 2007
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16. Aeropin from the extremophile Pyrobaculum aerophilum bypasses the serpin misfolding trap.
- Author
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Cabrita LD, Irving JA, Pearce MC, Whisstock JC, and Bottomley SP
- Subjects
- Amino Acid Sequence, Chromatography, Gel, Circular Dichroism, Disulfides chemistry, Hot Temperature, Hydrogen-Ion Concentration, Molecular Sequence Data, Protein Folding, Pyrobaculum chemistry, Serine Proteinase Inhibitors chemistry, Serpins chemistry
- Abstract
Serpins are metastable proteinase inhibitors. Serpin metastability drives both a large conformational change that is utilized during proteinase inhibition and confers an inherent structural flexibility that renders serpins susceptible to aggregation under certain conditions. These include point mutations (the basis of a number of important human genetic diseases), small changes in pH, and an increase in temperature. Many studies of serpins from mesophilic organisms have highlighted an inverse relationship: mutations that confer a marked increase in serpin stability compromise inhibitory activity. Here we present the first biophysical characterization of a metastable serpin from a hyperthermophilic organism. Aeropin, from the archaeon Pyrobaculum aerophilum, is both highly stable and an efficient proteinase inhibitor. We also demonstrate that because of high kinetic barriers, aeropin does not readily form the partially unfolded precursor to serpin aggregation. We conclude that stability and activity are not mutually exclusive properties in the context of the serpin fold, and propose that the increased stability of aeropin is caused by an unfolding pathway that minimizes the formation of an aggregation-prone intermediate ensemble, thereby enabling aeropin to bypass the misfolding fate observed with other serpins.
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- 2007
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17. SERPINB11 is a new noninhibitory intracellular serpin. Common single nucleotide polymorphisms in the scaffold impair conformational change.
- Author
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Askew DJ, Cataltepe S, Kumar V, Edwards C, Pace SM, Howarth RN, Pak SC, Askew YS, Brömme D, Luke CJ, Whisstock JC, and Silverman GA
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- Alleles, Amino Acid Sequence, Animals, Humans, Mice, Models, Molecular, Molecular Conformation, Molecular Sequence Data, Mutagenesis, Mutagenesis, Site-Directed, Recombinant Proteins chemistry, Sequence Homology, Amino Acid, Serpins chemistry, Tissue Distribution, Polymorphism, Single Nucleotide, Serpins physiology
- Abstract
SERPINB11, the last of 13 human clade B serpins to be described, gave rise to seven different isoforms. One cDNA contained a premature termination codon, two contained splice variants, and four contained full-length open reading frames punctuated by eight single nucleotide polymorphisms (SNPs). The SNPs encoded amino acid variants located within the serpin scaffold but not the reactive site loop (RSL). Although the mouse orthologue, Serpinb11, could inhibit trypsin-like peptidases, SERPINB11 showed no inhibitory activity. To determine whether the human RSL targeted a different class of peptidases or the serpin scaffold was unable to support inhibitory activity, we synthesized chimeric human and mouse proteins, in which the RSLs had been swapped. The human RSL served as a trypsin inhibitor when supported by mouse scaffold sequences. Conversely, the mouse RSL on the human scaffold showed no inhibitory activity. These findings suggested that variant residues in the SERPINB11 scaffold impaired serpin function. SDS-PAGE analysis supported this notion as RSL-cleaved SERPINB11 was unable to undergo the stressed-to-relaxed transition typical of inhibitory type serpins. Mutagenesis studies supported this hypothesis, since the reversion of amino acid sequences in helices D and I to those conserved in other clade B serpins partially restored the ability of SERPINB11 to form covalent complexes with trypsin. Taken together, these findings suggested that SERPINB11 SNPs encoded amino acids in the scaffold that impaired RSL mobility, and HapMap data showed that the majority of genomes in different human populations harbored these noninhibitory SERPINB11 alleles. Like several other serpin superfamily members, SERPINB11 has lost inhibitory activity and may have evolved a noninhibitory function.
- Published
- 2007
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18. The N terminus of the serpin, tengpin, functions to trap the metastable native state.
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Zhang Q, Buckle AM, Law RH, Pearce MC, Cabrita LD, Lloyd GJ, Irving JA, Smith AI, Ruzyla K, Rossjohn J, Bottomley SP, and Whisstock JC
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- Amino Acid Sequence, Amino Acid Substitution, Binding Sites, Conserved Sequence, Crystallization, Hydrogen Bonding, Hydrophobic and Hydrophilic Interactions, Models, Molecular, Molecular Sequence Data, Peptides chemistry, Peptides metabolism, Protein Binding, Protein Conformation, Protein Structure, Secondary, Protein Structure, Tertiary, Sequence Deletion, Serpins genetics, Spectrum Analysis, Raman, Thermoanaerobacter chemistry, Protein Folding, Serpins chemistry
- Abstract
Serpins fold to a metastable native state and are susceptible to undergoing spontaneous conformational change to more stable conformers, such as the latent form. We investigated conformational change in tengpin, an unusual prokaryotic serpin from the extremophile Thermoanaerobacter tengcongensis. In addition to the serpin domain, tengpin contains a functionally uncharacterized 56-amino-acid amino-terminal region. Deletion of this domain creates a variant--tengpinDelta51--which folds past the native state and readily adopts the latent conformation. Analysis of crystal structures together with mutagenesis studies show that the N terminus of tengpin protects a hydrophobic patch in the serpin domain and functions to trap tengpin in its native metastable state. A 13-amino-acid peptide derived from the N terminus is able to mimick the role of the N terminus in stabilizing the native state of tengpinDelta51. Therefore, the function of the N terminus in tengpin resembles protein cofactors that prevent mammalian serpins from spontaneously adopting the latent conformation.
- Published
- 2007
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19. Mechanisms of serpin dysfunction in disease.
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Kaiserman D, Whisstock JC, and Bird PI
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- Animals, Antithrombins chemistry, Humans, Mutation genetics, Protein Conformation, Serpins chemistry, Serpins genetics, alpha 1-Antitrypsin chemistry, Disease, Serpins metabolism
- Abstract
The serpin superfamily encompasses hundreds of proteins, spread across all kingdoms of life, linked by a common tertiary fold. This review focuses on five diseases caused by serpin dysfunction: variants of antithrombin III lose their ability to interact with heparin; the alpha1-antitrypsin Pittsburgh mutation causes a change in target proteinase; the alpha1-antitrypsin Z mutation and neuroserpin, polymerisation of which lead to cellular cytotoxicity; and a loss of maspin expression resulting in cancer.
- Published
- 2006
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20. Molecular gymnastics: serpin structure, folding and misfolding.
- Author
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Whisstock JC and Bottomley SP
- Subjects
- Animals, Arabidopsis Proteins chemistry, Arabidopsis Proteins metabolism, Humans, In Vitro Techniques, Models, Molecular, Molecular Structure, Multiprotein Complexes, Protein Conformation, Protein Folding, Thermodynamics, Thyroxine-Binding Proteins chemistry, Thyroxine-Binding Proteins metabolism, Serpins chemistry, Serpins metabolism
- Abstract
The native state of serpins represents a long-lived intermediate or metastable structure on the serpin folding pathway. Upon interaction with a protease, the serpin trap is sprung and the molecule continues to fold into a more stable conformation. However, thermodynamic stability can also be achieved through alternative, unproductive folding pathways that result in the formation of inactive conformations. Our increasing understanding of the mechanism of protease inhibition and the dynamics of native serpin structures has begun to reveal how evolution has harnessed the actual process of protein folding (rather than the final folded outcome) to elegantly achieve function. The cost of using metastability for function, however, is an increased propensity for misfolding.
- Published
- 2006
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21. X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation.
- Author
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McGowan S, Buckle AM, Irving JA, Ong PC, Bashtannyk-Puhalovich TA, Kan WT, Henderson KN, Bulynko YA, Popova EY, Smith AI, Bottomley SP, Rossjohn J, Grigoryev SA, Pike RN, and Whisstock JC
- Subjects
- Animals, Binding Sites, Cathepsin L, Cathepsins chemistry, Crystallography, X-Ray, Cysteine Endopeptidases chemistry, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Humans, Mutation, Nucleosomes metabolism, Protein Conformation, Serpins genetics, Serpins metabolism, Chromatin metabolism, DNA-Binding Proteins chemistry, Models, Molecular, Serpins chemistry
- Abstract
Most serpins are associated with protease inhibition, and their ability to form loop-sheet polymers is linked to conformational disease and the human serpinopathies. Here we describe the structural and functional dissection of how a unique serpin, the non-histone architectural protein, MENT (Myeloid and Erythroid Nuclear Termination stage-specific protein), participates in DNA and chromatin condensation. Our data suggest that MENT contains at least two distinct DNA-binding sites, consistent with its simultaneous binding to the two closely juxtaposed linker DNA segments on a nucleosome. Remarkably, our studies suggest that the reactive centre loop, a region of the MENT molecule essential for chromatin bridging in vivo and in vitro, is able to mediate formation of a loop-sheet oligomer. These data provide mechanistic insight into chromatin compaction by a non-histone architectural protein and suggest how the structural plasticity of serpins has adapted to mediate physiological, rather than pathogenic, loop-sheet linkages.
- Published
- 2006
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22. An overview of the serpin superfamily.
- Author
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Law RH, Zhang Q, McGowan S, Buckle AM, Silverman GA, Wong W, Rosado CJ, Langendorf CG, Pike RN, Bird PI, and Whisstock JC
- Subjects
- Genetic Predisposition to Disease, Humans, Mutation, Protein Conformation, Serine Proteinase Inhibitors classification, Serpins classification, Serine Proteinase Inhibitors chemistry, Serine Proteinase Inhibitors physiology, Serpins chemistry, Serpins physiology
- Abstract
Serpins are a broadly distributed family of protease inhibitors that use a conformational change to inhibit target enzymes. They are central in controlling many important proteolytic cascades, including the mammalian coagulation pathways. Serpins are conformationally labile and many of the disease-linked mutations of serpins result in misfolding or in pathogenic, inactive polymers.
- Published
- 2006
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23. The murine orthologue of human antichymotrypsin: a structural paradigm for clade A3 serpins.
- Author
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Horvath AJ, Irving JA, Rossjohn J, Law RH, Bottomley SP, Quinsey NS, Pike RN, Coughlin PB, and Whisstock JC
- Subjects
- Amino Acid Sequence, Animals, Brain metabolism, Circular Dichroism, Codon, Crystallography, X-Ray, Evolution, Molecular, Humans, Inflammation, Kinetics, Leukocyte Elastase metabolism, Leukocytes pathology, Ligands, Likelihood Functions, Mice, Models, Molecular, Molecular Conformation, Molecular Sequence Data, Phylogeny, Protein Conformation, Protein Structure, Secondary, Protein Structure, Tertiary, Recombinant Proteins chemistry, Salts pharmacology, Sequence Homology, Amino Acid, Serpins physiology, Temperature, Threonine chemistry, Time Factors, Trypsin chemistry, Trypsin pharmacology, Serpins chemistry
- Abstract
Antichymotrypsin (SERPINA3) is a widely expressed member of the serpin superfamily, required for the regulation of leukocyte proteases released during an inflammatory response and with a permissive role in the development of amyloid encephalopathy. Despite its biological significance, there is at present no available structure of this serpin in its native, inhibitory state. We present here the first fully refined structure of a murine antichymotrypsin orthologue to 2.1 A, which we propose as a template for other antichymotrypsin-like serpins. A most unexpected feature of the structure of murine serpina3n is that it reveals the reactive center loop (RCL) to be partially inserted into the A beta-sheet, a structural motif associated with ligand-dependent activation in other serpins. The RCL is, in addition, stabilized by salt bridges, and its plane is oriented at 90 degrees to the RCL of antitrypsin. A biochemical and biophysical analysis of this serpin demonstrates that it is a fast and efficient inhibitor of human leukocyte elastase (ka: 4 +/- 0.9 x 10(6) m(-1) s(-)1) and cathepsin G (ka: 7.9 +/- 0.9 x 10(5) m(-1) s(-)1) giving a spectrum of activity intermediate between that of human antichymotrypsin and human antitrypsin. An evolutionary analysis reveals that residues subject to positive selection and that have contributed to the diversity of sequences in this sub-branch (A3) of the serpin superfamily are essentially restricted to the P4-P6' region of the RCL, the distal hinge, and the loop between strands 4B and 5B.
- Published
- 2005
- Full Text
- View/download PDF
24. Serpins 2005 - fun between the beta-sheets. Meeting report based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia).
- Author
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Whisstock JC, Bottomley SP, Bird PI, Pike RN, and Coughlin P
- Subjects
- Animals, Humans, Protein Structure, Secondary, Serine Proteinase Inhibitors chemistry, Serine Proteinase Inhibitors metabolism, Serpins chemistry, Serpins metabolism
- Abstract
Serpins are the largest family of protease inhibitors and are fundamental for the control of proteolysis in multicellular eukaryotes. Most eukaryote serpins inhibit serine or cysteine proteases, however, noninhibitory members have been identified that perform diverse functions in processes such as hormone delivery and tumour metastasis. More recently inhibitory serpins have been identified in prokaryotes and unicellular eukaryotes, nevertheless, the precise molecular targets of these molecules remains to be identified. The serpin mechanism of protease inhibition is unusual and involves a major conformational rearrangement of the molecule concomitant with a distortion of the target protease. As a result of this requirement, serpins are susceptible to mutations that result in polymerization and conformational diseases such as the human serpinopathies. This review reports on recent major discoveries in the serpin field, based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia).
- Published
- 2005
- Full Text
- View/download PDF
25. The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix.
- Author
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Law RH, Irving JA, Buckle AM, Ruzyla K, Buzza M, Bashtannyk-Puhalovich TA, Beddoe TC, Nguyen K, Worrall DM, Bottomley SP, Bird PI, Rossjohn J, and Whisstock JC
- Subjects
- Animals, Binding Sites, Cathepsin L, Cathepsins chemistry, Chickens, Circular Dichroism, Cysteine Endopeptidases chemistry, Extracellular Matrix, Genes, Tumor Suppressor, Homozygote, Humans, Mice, Models, Molecular, Plasmids metabolism, Protein Binding, Protein Conformation, Protein Structure, Secondary, Rats, Recombinant Proteins chemistry, Spectrophotometry, Static Electricity, Temperature, Xenopus, Crystallography, X-Ray methods, Serpins chemistry
- Abstract
Maspin is a serpin that acts as a tumor suppressor in a range of human cancers, including tumors of the breast and lung. Maspin is crucial for development, because homozygous loss of the gene is lethal; however, the precise physiological role of the molecule is unclear. To gain insight into the function of human maspin, we have determined its crystal structure in two similar, but non-isomorphous crystal forms, to 2.1- and 2.8-A resolution, respectively. The structure reveals that maspin adopts the native serpin fold in which the reactive center loop is expelled fully from the A beta-sheet, makes minimal contacts with the core of the molecule, and exhibits a high degree of flexibility. A buried salt bridge unique to maspin orthologues causes an unusual bulge in the region around the D and E alpha-helices, an area of the molecule demonstrated in other serpins to be important for cofactor recognition. Strikingly, the structural data reveal that maspin is able to undergo conformational change in and around the G alpha-helix, switching between an open and a closed form. This change dictates the electrostatic character of a putative cofactor binding surface and highlights this region as a likely determinant of maspin function. The high resolution crystal structure of maspin provides a detailed molecular framework to elucidate the mechanism of function of this important tumor suppressor.
- Published
- 2005
- Full Text
- View/download PDF
26. The high resolution crystal structure of a native thermostable serpin reveals the complex mechanism underpinning the stressed to relaxed transition.
- Author
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Fulton KF, Buckle AM, Cabrita LD, Irving JA, Butcher RE, Smith I, Reeve S, Lesk AM, Bottomley SP, Rossjohn J, and Whisstock JC
- Subjects
- Binding Sites, Circular Dichroism, Cloning, Molecular, Crystallography, X-Ray, Hydrogen Bonding, Kinetics, Models, Molecular, Mutagenesis, Site-Directed, Protein Binding, Protein Conformation, Protein Structure, Secondary, Protein Structure, Tertiary, Serpins physiology, Spectrophotometry, Temperature, Thermodynamics, Serpins chemistry, Streptomycetaceae metabolism
- Abstract
Serpins fold into a native metastable state and utilize a complex conformational change to inhibit target proteases. An undesirable result of this conformational flexibility is that most inhibitory serpins are heat sensitive, forming inactive polymers at elevated temperatures. However, the prokaryote serpin, thermopin, from Thermobifida fusca is able to function in a heated environment. We have determined the 1.8 A x-ray crystal structure of thermopin in the native, inhibitory conformation. A structural comparison with the previously determined 1.5 A structure of cleaved thermopin provides detailed insight into the complex mechanism of conformational change in serpins. Flexibility in the shutter region and electrostatic interactions at the top of the A beta-sheet (the breach) involving the C-terminal tail, a unique structural feature of thermopin, are postulated to be important for controlling inhibitory activity and triggering conformational change, respectively, in the native state. Here we have discussed the structural basis of how this serpin reconciles the thermodynamic instability necessary for function with the stability required to withstand elevated temperatures.
- Published
- 2005
- Full Text
- View/download PDF
27. The human serpin proteinase inhibitor-9 self-associates at physiological temperatures.
- Author
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Benning LN, Whisstock JC, Sun J, Bird PI, and Bottomley SP
- Subjects
- Benzothiazoles, Dementia metabolism, Emphysema metabolism, Humans, Microscopy, Electron, Multiprotein Complexes ultrastructure, Protein Denaturation, Protein Structure, Tertiary, Serpins metabolism, Serpins ultrastructure, Spectrometry, Fluorescence, Structure-Activity Relationship, Thiazoles chemistry, Thrombosis metabolism, Multiprotein Complexes chemistry, Protein Folding, Serpins chemistry
- Abstract
The metastable serpin architecture is perturbed by extremes of temperature, pH, or changes in primary sequence resulting in the formation of inactive, polymeric conformations. Polymerization of a number of human serpins in vivo leads to diseases such as emphysema, thrombosis, and dementia, and in these cases mutations are present within the gene encoding the aggregating protein. Here we show that aggregation of the human serpin, proteinase inhibitor-9 (PI-9), occurs under physiological conditions, and forms aggregates that are morphologically distinct from previously characterized serpin polymers. Incubation of monomeric PI-9 at 37 degrees C leads to the rapid formation of aggregated PI-9. Using a variety of spectroscopic methods we analyzed the nature of the structures formed after incubation at 37 degrees C. Electron microscopy showed that PI-9 forms ordered circular and elongated-type aggregates, which also bind the fluorescent dye Thioflavin T. Our data show that in vitro wild-type PI-9 forms aggregates at physiological temperatures. The biological implications of PI-9 aggregates at physiological temperatures are discussed.
- Published
- 2004
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28. Computational analysis of evolution and conservation in a protein superfamily.
- Author
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Irving JA, Askew DJ, and Whisstock JC
- Subjects
- Conserved Sequence genetics, Databases, Nucleic Acid, Databases, Protein, Imaging, Three-Dimensional, Mutation genetics, Phylogeny, Protein Structure, Secondary, Sequence Alignment methods, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Serpins chemistry, Software, Software Design, Structural Homology, Protein, Computational Biology methods, Evolution, Molecular, Serpins genetics
- Abstract
Many gene superfamilies have hundreds or thousands of members and hence pose a significant challenge when performing a large-scale phylogenetic analysis. Derivation of the most accurate alignment possible and inference of evolutionary relationships (with an appropriate measure of confidence) are significant "bottlenecks" in the process. A generally applicable strategy is outlined for identifying and aligning sequences, performing simple analysis of the resulting alignment, and inferring evolutionary relationships. Reference is made to the serpin superfamily. The 'partition cluster' method, a relatively rapid technique for extracting underlying associations from phylogenetic bootstrap trees, is also presented.
- Published
- 2004
- Full Text
- View/download PDF
29. Hurpin is a selective inhibitor of lysosomal cathepsin L and protects keratinocytes from ultraviolet-induced apoptosis.
- Author
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Welss T, Sun J, Irving JA, Blum R, Smith AI, Whisstock JC, Pike RN, von Mikecz A, Ruzicka T, Bird PI, and Abts HF
- Subjects
- Amino Acid Sequence, Apoptosis drug effects, Cathepsin L, Cathepsins metabolism, Cysteine Endopeptidases metabolism, Cysteine Proteinase Inhibitors biosynthesis, Cysteine Proteinase Inhibitors chemistry, Cysteine Proteinase Inhibitors genetics, Cytosol enzymology, Humans, Keratinocytes cytology, Keratinocytes metabolism, Keratinocytes radiation effects, Kinetics, Models, Molecular, Molecular Sequence Data, Mutation, Recombinant Proteins biosynthesis, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins pharmacology, Sequence Homology, Amino Acid, Serpins biosynthesis, Serpins chemistry, Serpins genetics, Ultraviolet Rays, Yeasts metabolism, Apoptosis radiation effects, Cathepsins antagonists & inhibitors, Cysteine Proteinase Inhibitors pharmacology, Keratinocytes drug effects, Lysosomes enzymology, Serpins pharmacology
- Abstract
Hurpin (headpin/PI13/serpinB13) is an intracellular, differentially spliced member of the serpin superfamily that has been linked to differentiation and apoptosis of human keratinocytes. It is transiently downregulated by UV light and overexpressed in psoriatic skin lesions. Although it has all of the features of an inhibitory serpin, a productive interaction between hurpin and a proteinase has not yet been reported. Here we demonstrate that hurpin is a potent and selective inhibitor of the archetypal lysosomal cysteine proteinase cathepsin L (catL). Recombinant hurpin inhibits human catL with a stoichiometry of inhibition (SI) of 1.7 and a rate constant k(assoc) of (4.6 +/- 0.14) x 10(5) M(-1) s(-1). It inefficiently inhibits catV and does not inhibit papain, catB, or catK. To investigate the inhibitory mechanism, we determined the P1-P1' bond in the reactive center loop cleaved by catL ((356)Thr-(357)Ser) and expressed variants in which the proximal hinge, P1 residue, or differentially spliced CD loop was mutated. The results of assays using these proteins suggest that inhibition of catL by hurpin occurs via the conventional serpin inhibitory mechanism and that the CD loop plays no role in the process. Finally, it was found that the majority of hurpin is cytosolic and that its overexpression in human keratinocytes confers resistance to UV-induced apoptosis. Given that lysosomal disruption, release of catL, and catL-mediated caspase activation are known to occur in response to cellular stress, we propose that a physiological role of hurpin is to protect epithelial cells from ectopic catL.
- Published
- 2003
- Full Text
- View/download PDF
30. The 1.5 A crystal structure of a prokaryote serpin: controlling conformational change in a heated environment.
- Author
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Irving JA, Cabrita LD, Rossjohn J, Pike RN, Bottomley SP, and Whisstock JC
- Subjects
- Amino Acid Sequence, Bacterial Proteins genetics, Bacterial Proteins metabolism, Crystallography, X-Ray, Enzyme Stability, Humans, Models, Molecular, Molecular Sequence Data, Protein Denaturation, Sequence Alignment, Serine Proteinase Inhibitors genetics, Serine Proteinase Inhibitors metabolism, Serpins genetics, Serpins metabolism, alpha 1-Antitrypsin chemistry, alpha 1-Antitrypsin genetics, Actinomycetales chemistry, Bacterial Proteins chemistry, Hot Temperature, Protein Conformation, Serine Proteinase Inhibitors chemistry, Serpins chemistry
- Abstract
Serpins utilize conformational change to inhibit target proteinases; the price paid for this conformational flexibility is that many undergo temperature-induced polymerization. Despite this thermolability, serpins are present in the genomes of thermophilic prokaryotes, and here we characterize the first such serpin, thermopin. Thermopin is a proteinase inhibitor and, in comparison with human alpha(1)-antitrypsin, possesses enhanced stability at 60 degrees C. The 1.5 A crystal structure reveals novel structural features in regions implicated in serpin folding and stability. Thermopin possesses a C-terminal "tail" that interacts with the top of the A beta sheet and plays an important role in the folding/unfolding of the molecule. These data provide evidence as to how this unusual serpin has adapted to fold and function in a heated environment.
- Published
- 2003
- Full Text
- View/download PDF
31. Serpins in prokaryotes.
- Author
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Irving JA, Steenbakkers PJ, Lesk AM, Op den Camp HJ, Pike RN, and Whisstock JC
- Subjects
- Amino Acid Sequence, Animals, Humans, Molecular Sequence Data, Phylogeny, Protein Conformation, Sequence Homology, Amino Acid, Serpins genetics, Species Specificity, Structure-Activity Relationship, Evolution, Molecular, Prokaryotic Cells chemistry, Serpins chemistry
- Abstract
Members of the serpin (serine proteinase inhibitor) superfamily have been identified in higher multicellular eukaryotes (plants and animals) and viruses but not in bacteria, archaea, or fungi. Thus, the ancestral serpin and the origin of the serpin inhibitory mechanism remain obscure. In this study we characterize 12 serpin-like sequences in the genomes of prokaryotic organisms, extending this protein family to all major branches of life. Notably, these organisms live in dramatically different environments and some are evolutionarily distantly related. A sequence-based analysis suggests that all 12 serpins are inhibitory. Despite considerable sequence divergence between the proteins, in four of the 12 sequences the region of the serpin that determines proteinase specificity is highly conserved, indicating that these inhibitors are likely to share a common target. Inhibitory serpins are typically prone to polymerization upon heating; thus, the existence of serpins in the moderate thermophilic bacterium Thermobifida fusca, the thermophilic bacterium Thermoanaerobacter tengcongensis, and the hyperthermophilic archaeon Pyrobaculum aerophilum is of particular interest. Using molecular modeling, we predict the means by which heat stability in the latter protein may be achieved without compromising inhibitory activity.
- Published
- 2002
- Full Text
- View/download PDF
32. Serpins: finely balanced conformational traps.
- Author
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Pike RN, Bottomley SP, Irving JA, Bird PI, and Whisstock JC
- Subjects
- Amino Acid Motifs, Animals, Catalysis, Genetic Diseases, Inborn enzymology, Genetic Diseases, Inborn genetics, Humans, Intracellular Fluid enzymology, Mice, Mice, Transgenic, Models, Molecular, Plasminogen Activator Inhibitor 1 chemistry, Plasminogen Activator Inhibitor 1 physiology, Point Mutation, Protein Binding, Protein Conformation, Protein Folding, Protein Structure, Secondary, Serine Proteinase Inhibitors metabolism, Serine Proteinase Inhibitors pharmacology, Serpins genetics, Serpins metabolism, Structure-Activity Relationship, Serpins chemistry
- Abstract
Serine protease inhibitors (serpins) play very important roles in the maintenance of various physiologically important systems. As knowledge of the workings of proteins of this family grows, new understanding is gained of the mechanisms by which they inhibit target proteases, using conformational changes for which the structure of serpins is uniquely adapted. This finely balanced system is utilized to healthy benefit in the control of serpin function by modulators, arguably the most striking examples of which occur in the control of proteolytic cascades, such as the coagulation system. Serpins also play very important intracellular roles: one example is the protection of immune cells from their own cytotoxic proteases. The finely balanced serpin mechanism also means that it is prone to disastrous consequences if mutations should occur in vital positions in the serpin structure. Many examples of disease-associated mutations have been shown, which has the dual effect of highlighting how important these molecules are in the maintenance of health and the fine balance that must be maintained in order to preserve their active, inhibitory conformation.
- Published
- 2002
- Full Text
- View/download PDF
33. Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering alpha(1)-antitrypsin to inhibit cathepsin proteases.
- Author
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Irving JA, Pike RN, Dai W, Brömme D, Worrall DM, Silverman GA, Coetzer TH, Dennison C, Bottomley SP, and Whisstock JC
- Subjects
- Amino Acid Sequence, Antigens, Neoplasm chemistry, Cysteine Proteinase Inhibitors pharmacology, Humans, Kinetics, Molecular Sequence Data, Mutagenesis, Site-Directed, Structure-Activity Relationship, Cathepsins antagonists & inhibitors, Cysteine Proteinase Inhibitors chemistry, Papain antagonists & inhibitors, Serpins chemistry, Serpins pharmacology, alpha 1-Antitrypsin chemistry
- Abstract
The closely related serpins squamous cell carcinoma antigen-1 and -2 (SCCA-1 and -2, respectively) are capable of inhibiting cysteine proteases of the papain superfamily. To ascertain whether the ability to inhibit cysteine proteases is an intrinsic property of serpins in general, the reactive center loop (RCL) of the archetypal serine protease inhibitor alpha(1)-antitrypsin was replaced with that of SCCA-1. It was found that this simple substitution could convert alpha(1)-antitrypsin into a cysteine protease inhibitor, albeit an inefficient one. The RCL of SCCA-1 is three residues longer than that of alpha(1)-antitrypsin, and therefore, the effect of loop length on the cysteine protease inhibitory activity was investigated. Mutants in which the RCL was shortened by one, two, or three residues were effective inhibitors with second-order rate constants of 10(5)-10(7) M(-)(1) s(-)(1). In addition to loop length, the identity of the cysteine protease was of considerable importance, since the chimeric molecules inhibited cathepsins L, V, and K efficiently, but not papain or cathepsin B. By testing complexes between an RCL-mimicking peptide and the mutants, it was found that the formation of a stable serpin-cysteine protease complex and the inhibition of a cysteine protease were both critically dependent on RCL insertion. The results strongly indicate that the serpin body is intrinsically capable of supporting cysteine protease inhibition, and that the complex with a papain-like cysteine protease would be expected to be analogous to that seen with serine proteases.
- Published
- 2002
- Full Text
- View/download PDF
34. Probing the role of the F-helix in serpin stability through a single tryptophan substitution.
- Author
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Cabrita LD, Whisstock JC, and Bottomley SP
- Subjects
- Alanine, Amino Acid Sequence, Amino Acid Substitution, Drug Stability, Guanidine, Models, Molecular, Mutagenesis, Site-Directed, Protein Conformation, Protein Denaturation, Protein Structure, Secondary, Recombinant Proteins chemistry, Spectrometry, Fluorescence, alpha 1-Antitrypsin chemistry, Serpins chemistry, Tryptophan
- Abstract
Serpins form loop-sheet polymers through the formation of a partially folded intermediate. Through mutagenesis and biophysical analysis, we have probed the conformational stability of the F-helix, demonstrating that it is almost completely unfolded in the intermediate state. The replacement of Tyr160 on the F-helix of alpha1-antitrypsin to alanine results in the loss of a conserved hydrogen bond that dramatically reduces the stability of the protein to both heat and solvent denaturation, indicating the importance of Tyr160 in the stability of the molecule. The mutation of Tyr160 to a tryptophan residue, within a fluorescently silent variant of alpha1-antitrypsin, results in a fully active, stable serpin. Fluorescence analysis of the equilibrium unfolding behavior of this variant indicates that the F-helix is highly disrupted in the intermediate conformation. Iodide quenching experiments demonstrate that the tryptophan residue is exposed to a similar extent in both the intermediate and unfolded states. Cumulatively, these data indicate that the F-helix plays an important role in controlling the early conformational changes involved in alpha1-antitrypsin unfolding. The implications of these data on both alpha1-antitrypsin function and misfolding are discussed.
- Published
- 2002
- Full Text
- View/download PDF
35. The serpin SQN-5 is a dual mechanistic-class inhibitor of serine and cysteine proteinases.
- Author
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Al-Khunaizi M, Luke CJ, Askew YS, Pak SC, Askew DJ, Cataltepe S, Miller D, Mills DR, Tsu C, Brömme D, Irving JA, Whisstock JC, and Silverman GA
- Subjects
- Amino Acid Sequence, Animals, Antigens, Neoplasm chemistry, Antigens, Neoplasm metabolism, Binding, Competitive, Cathepsins metabolism, Cysteine Endopeptidases drug effects, Humans, Kinetics, Mice, Molecular Sequence Data, Phylogeny, Protease Inhibitors chemistry, Protease Inhibitors classification, Sequence Homology, Amino Acid, Serine Endopeptidases drug effects, Serpins chemistry, Serpins classification, Cysteine Endopeptidases metabolism, Protease Inhibitors pharmacology, Serine Endopeptidases metabolism, Serpins pharmacology
- Abstract
SQN-5 is a mouse serpin that is highly similar to the human serpins SCCA1 (SERPINB3) and SCCA2 (SERPINB4). Previous studies characterizing the biochemical activity of SQN-5 showed that this serpin, like SCCA2, inhibited the chymotrypsin-like enzymes mast cell chymase and cathepsin G. Using an expanded panel of papain-like cysteine proteinases, we now show that SQN-5, like SCCA1, inhibited cathepsins K, L, S, and V but not cathepsin B or H. These interactions were characterized by stoichiometries of inhibition that were nearly 1:1 and second-order rate constants of >10(4) M(-1) s(-1). Reactive site loop (RSL) cleavage analysis showed that SQN-5 employed different reactive centers to neutralize the serine and cysteine proteinases. To our knowledge, this is the first serpin that serves as a dual inhibitor of both chymotrypsin-like serine and the papain-like cysteine proteinases by employing an RSL-dependent inhibitory mechanism. The ability of serpins to inhibit both serine and/or papain-like cysteine proteinases may not be a recent event in mammalian evolution. Phylogenetic studies suggested that the SCCA and SQN genes evolved from a common ancestor approximately 250-280 million years ago. When the fact that mammals and birds diverged approximately 310 million years ago is considered, an ancestral SCCA/SQN-like serpin with dual inhibitory activity may be present in many mammalian genomes.
- Published
- 2002
- Full Text
- View/download PDF
36. Characterization and functional analysis of Serp3: a novel myxoma virus-encoded serpin involved in virulence.
- Author
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Guerin JL, Gelfi J, Camus C, Delverdier M, Whisstock JC, Amardeihl MF, Py R, Bertagnoli S, and Messud-Petit F
- Subjects
- Amino Acid Motifs, Amino Acid Sequence, Animals, Apoptosis, Cell Line, Gene Deletion, Lymph Nodes immunology, Lymph Nodes pathology, Lymph Nodes virology, Models, Molecular, Molecular Sequence Data, Myxoma virus genetics, Myxoma virus growth & development, Myxoma virus metabolism, Myxomatosis, Infectious pathology, Myxomatosis, Infectious virology, Open Reading Frames genetics, Parotid Gland immunology, Parotid Gland pathology, Parotid Gland virology, Promoter Regions, Genetic genetics, Protein Conformation, RNA, Viral analysis, RNA, Viral genetics, Rabbits, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins metabolism, Serpins chemistry, Serpins genetics, Survival Rate, Viral Load, Viral Proteins chemistry, Viral Proteins genetics, Viral Proteins metabolism, Virulence genetics, alpha 1-Antitrypsin chemistry, Myxoma virus pathogenicity, Serpins metabolism
- Abstract
Myxoma virus (MV), a member of the family Poxviridae, is the causative agent of myxomatosis, a fatal disease of the European rabbit. The MV genome is a linear, double-stranded DNA molecule that encodes several factors important for evasion of the host immune system. Sequencing the right-end region of the MV genome identified an 801 bp open reading frame (ORF) encoding a polypeptide that belongs to the serpin superfamily. To date, two MV-encoded serpins have been characterized: SERP-1 binds to several targets and is an anti-inflammatory molecule, whereas Serp2 is essential for virus virulence and has both anti-inflammatory and anti-apoptotic effects. Thus, Serp3 is the third MV-encoded serpin. DNA sequence analysis of Serp3 indicated a similarity to poxvirus late promoters, which was confirmed by mRNA expression analysis. Serp3 has an atypical serpin motif and has significant sequence deletions as compared to most cellular and viral serpins. However, molecular modelling studies suggested that Serp3 can retain the overall serpin fold. Insertional inactivation of the serp3 ORF led to a significant attenuation of virulence in vivo (as measured by the increase in survival of infected rabbits) and limited dissemination of the virus to secondary sites of infection. In rabbits infected with a Serp3 deletion mutant (MV-Serp3(-)), the main histopathological feature is the absence of secondary myxomas. Both wild-type MV and MV-Serp3(-) replicate at comparable levels in vivo. Serp3 may represent a significant virulence factor of MV and probably acts in synergy with other viral proteins.
- Published
- 2001
- Full Text
- View/download PDF
37. Serpins 2005 – fun between the β-sheets.
- Author
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Whisstock, James C., Bottomley, Stephen P., Bird, Phillip I., Pike, Robert N., and Coughlin, Paul
- Subjects
SERPINS ,SERINE proteinase inhibitors ,PROTEASE inhibitors ,PROTEOLYSIS ,ENZYME inhibitors ,PROTEIN metabolism - Abstract
Serpins are the largest family of protease inhibitors and are fundamental for the control of proteolysis in multicellular eukaryotes. Most eukaryote serpins inhibit serine or cysteine proteases, however, noninhibitory members have been identified that perform diverse functions in processes such as hormone delivery and tumour metastasis. More recently inhibitory serpins have been identified in prokaryotes and unicellular eukaryotes, nevertheless, the precise molecular targets of these molecules remains to be identified. The serpin mechanism of protease inhibition is unusual and involves a major conformational rearrangement of the molecule concomitant with a distortion of the target protease. As a result of this requirement, serpins are susceptible to mutations that result in polymerization and conformational diseases such as the human serpinopathies. This review reports on recent major discoveries in the serpin field, based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia). [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
38. The role of strand 1 of the C β-sheet in the structure and function of α1-antitrypsin.
- Author
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Bottomley, Stephen P., Lawrenson, Isobel D., Tew, Deborah, Dai, Weiwen, Whisstock, James C., and Pike, Robert N.
- Abstract
Serpins inhibit cognate serine proteases involved in a number of important processes including blood coagulation and inflammation. Consequently, loss of serpin function or stability results in a number of disease states. Many of the naturally occurring mutations leading to disease are located within strand 1 of the C β-sheet of the serpin. To ascertain the structural and functional importance of each residue in this strand, which constitutes the so-called distal hinge of the reactive center loop of the serpin, an alanine scanning study was carried out on recombinant α
1 -antitrypsin Pittsburgh mutant (P1 = Arg). Mutation of the P10′ position had no effect on its inhibitory properties towards thrombin. Mutations to residues P7′ and P9′ caused these serpins to have an increased tendency to act as substrates rather than inhibitors, while mutations at P6′ and P8′ positions caused the serpin to behave almost entirely as a substrate. Mutations at the P6′ and P8′ residues of the C β-sheet, which are buried in the hydrophobic core in the native structure, caused the serpin to become highly unstable and polymerize much more readily. Thus, P6′ and P8′ mutants of α1 -antitrypsin had melting temperatures 14 degrees lower than wild-type α1 -antitrypsin. These results indicate the importance of maintaining the anchoring of the distal hinge to both the inhibitory mechanism and stability of serpins, the inhibitory mechanism being particularly sensitive to any perturbations in this region. The results of this study allow more informed analysis of the effects of mutations found at these positions in disease-associated serpin variants. [ABSTRACT FROM AUTHOR]- Published
- 2001
- Full Text
- View/download PDF
39. Mapping the binding site of C1-inhibitor for polyanion cofactors.
- Author
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Hor, Lilian, Pan, Jing, Whisstock, James C., Pike, Robert N., and Wijeyewickrema, Lakshmi C.
- Subjects
- *
BINDING sites , *POLYANIONS , *HEPARIN , *POLYPHOSPHATES , *VITAL statistics , *BLOOD coagulation , *MOLECULES - Abstract
• C1-inhibitor plays a vital anti-inflammatory role in the body by controlling pro-inflammatory pathways such as complement and coagulation. • The inhibitor's action is enhanced in the presence of polyanionic cofactors, such as heparin and polyphosphate • Co factor binding site of the serpin was mapped and shown to be vital for rate enhancement • Findings provide new insight into the mechanism of interaction of co factors with C1-inhibitor. The serpin, C1-inhibitor (also known as SERPING1), plays a vital anti-inflammatory role in the body by controlling pro-inflammatory pathways such as complement and coagulation. The inhibitor's action is enhanced in the presence of polyanionic cofactors, such as heparin and polyphosphate, by increasing the rate of association with key enzymes such as C1s of the classical pathway of complement. The cofactor binding site of the serpin has never been mapped. Here we show that residues Lys284, Lys285 and Arg287 of C1-inhibitor play key roles in binding heparin and delivering the rate enhancement seen in the presence of polyanions and thus most likely represent the key cofactor binding residues for the serpin. We also show that simultaneous binding of the anion binding site of C1s by the polyanion is required to deliver the rate enhancement. Finally, we have shown that it is unlikely that the two positively charged zones of C1-inhibitor and C1s interact in the encounter complex between molecules as ablation of the charged zones did not in itself deliver a rate enhancement as might have been expected if the zones interacted. These insights provide crucial information as to the mechanism of action of this key serpin in the presence and absence of cofactor molecules. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
40. Structural biology: Serpins' mystery solved.
- Author
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Whisstock, James C. and Bottomley, Stephen P.
- Subjects
- *
SERINE proteinase inhibitors , *SERPINS , *POLYMERS , *NEURODEGENERATION , *DEGENERATION (Pathology) , *AMYLOID , *PARKINSON'S disease , *HUNTINGTON disease - Abstract
The article discusses that polymers of serpin proteins underlie diseases, including neurodegenerative disorders. It is said that Alois Alzheimer has offered a description on the abnormal aggregates of the amyloid protein in the brain of a patient who has died of dementia. The known conformational diseases include prion diseases, Parkinson's disease, Huntington's disease and several disorders known as serpinopathies. It is mentioned that the serpin polymer is correctly folded and resembles the relaxed serpin, which does not evoke protective signalling pathway.
- Published
- 2008
- Full Text
- View/download PDF
41. DNA Accelerates the Inhibition of Human Cathepsin V by Serpins.
- Author
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Poh Chee Ong, McGowan, Sheena, Pearce, Mary C., Irving, James A., Wan-Ting Kan, Grigoryev, Sergei A., Turk, Boris, Silverman, Gary A., Brix, Klaudia, Bottomley, Stephen P., Whisstock, James C., and Pike, Robert N.
- Subjects
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CYSTEINE proteinases , *PROTEOLYTIC enzymes , *DNA , *NUCLEIC acids , *SERPINS , *CELL nuclei - Abstract
A balance between proteolytic activity and protease inhibition is crucial to the appropriate function of many biological processes. There is mounting evidence for the presence of both papain-like cysteine proteases and serpins with a corresponding inhibitory activity in the nucleus. Well characterized examples of cofactors fine tuning serpin activity in the extracellular milieu are known, but such modulation has not been studied for protease-serpin interactions within the cell. Accordingly, we present an investigation into the effect of a DNA-rich environment on the interaction between model serpins (MENT and SCCA-1), cysteine proteases (human cathepsin V and human cathepsin L), and cystatin A. DNA was indeed found to accelerate the rate at which MENT inhibited cathepsin V, a human orthologue of mammalian cathepsin L, up to 50-fold, but unexpectedly this effect was primarily effected via the protease and secondarily by the recruitment of the DNA as a "template" onto which cathepsin V and MENT are bound. Notably, the protease-mediated effect was found to correspond both with an altered substrate turnover and a conformational change within the protease. Consistent with this, cystatin inhibition, which relies on occlusion of the active site rather than the substrate-like behavior of serpins, was unaltered by DNA. This represents the first example of modulation of serpin inhibition of cysteine proteases by a co-factor and reveals a mechanism for differential regulation of cathepsin proteolytic activity in a DNA-rich environment. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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