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The article discusses the optimization of the cytocompatible silica coating conditions for screening of mammalian HeLa cells in silicification conditions. Topics discussed include the stringent selection of material for the manipulation of mammalian cells and the process coating of individual HeLa cells with silica in cytocompatible conditions.

The manipulation of cell surfaces in anchorage‐dependent cells can help overcome difficulties presented during cell handling and application. Silica‐based protective mechanisms existing in free‐floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose‐derived mesenchymal stem cells (hASCs) are partially coated with a hard silica layer that operates as a supporting platform for individual cells in suspension. Inspired by the organic templates involved in biosilicification, a novel chitosan (CHT) derivative displaying fully natural quaternary amine moieties is synthetized via a rapid, one‐pot strategy. Silicification is promoted on individual hASCs surfaces via a two‐step process: a priming step onto previously adhered cell with this CHT derivative, followed by a biocompatible sol–gel process. hASCs holding a silica backpack exhibit enhanced cell survival in suspension conditions and can spread and acquire a more adherent phenotype. This new protocol for cell‐surface modification also provides a new generation of hybrid materials with functionalization of the silica backpack, which can be applied to different areas such as tissue engineering, biosensing, drug delivery, and targeted cell‐based therapies. [ABSTRACT FROM AUTHOR]

The article discusses a study which developed a method for fabricating silica thin films on gold substrates using a rationally designed molecule as a catalytic templates. Topics covered include the synthesis of a catalytic molecule, histidine-conjugated thiol, the experimental procedure for the formation of silica thin films, and the catalytic activity of histidine-terminated self-assembled monolayers (His-SAM) compared with that of amine-SAM.

In the area of cell-surface engineering with nanomaterials, the metabolic and functional activities of the encapsulated cells are manipulated and controlled by various parameters of the artificial shells that encase the cells, such as stiffness and elasticity, thickness, and porosity. The mechanical durability and physicochemical stability of inorganic shells prove superior to layer-by-layer-based organic shells with regard to cytoprotection, but it has been difficult to vary the parameters of inorganic shells including their thickness. In this work, we combine the layer-by-layer technique with a process of bioinspired silicification to control the thickness of the silica shells that encapsulate yeast Saccharomyces cerevisiae cells individually, and investigate the thickness-dependent microbial growth. [ABSTRACT FROM AUTHOR]

The cytoprotective coating of physicochemically labile mammalian cells with a durable material has potential applications in cell-based sensors, cell therapy, and regenerative medicine, as well as providing a platform for fundamental single-cell studies in cell biology. In this work, HeLa cells in suspension were individually coated with silica in a cytocompatible fashion through bioinspired silicification. The silica coating greatly enhanced the resistance of the HeLa cells to enzymatic attack by trypsin and the toxic compound poly- (allylamine hydrochloride), while suppressing cell division in a controlled fashion. This bioinspired cytocompatible strategy for single-cell coating was also applied to NIH 3T3 fibroblasts and Jurkat cells. [ABSTRACT FROM AUTHOR]

The cytoprotective coating of physicochemically labile mammalian cells with a durable material has potential applications in cell-based sensors, cell therapy, and regenerative medicine, as well as providing a platform for fundamental single-cell studies in cell biology. In this work, HeLa cells in suspension were individually coated with silica in a cytocompatible fashion through bioinspired silicification. The silica coating greatly enhanced the resistance of the HeLa cells to enzymatic attack by trypsin and the toxic compound poly- (allylamine hydrochloride), while suppressing cell division in a controlled fashion. This bioinspired cytocompatible strategy for single-cell coating was also applied to NIH 3T3 fibroblasts and Jurkat cells. [ABSTRACT FROM AUTHOR]

Hard‐shell case: Using a (RKK)4D8 peptide allows mineralization to occur under cytocompatible conditions. Thus individual Chlorella cells could be encapsulated within a SiO2–TiO2 nanoshell with high cell viability (87 %). The encapsulated Chlorella showed an almost threefold increase in their thermo‐tolerance after 2 h at 45 °C. [ABSTRACT FROM AUTHOR]

Keywords: advanced cell-material interactions; cell partial coatings; cell-based materials; organic-inorganic hybrid composites; sol-gel processes EN advanced cell-material interactions cell partial coatings cell-based materials organic-inorganic hybrid composites sol-gel processes 1 1 1 07/19/21 20210701 NES 210701 In article number 2009619, Tiago R. Correia, João F. Mano, and co-workers develop a new type of hybrid cells. Individual human adipose-derived mesenchymal stem cells are partially coated with a hard silica layer and display enhanced single-cell survival and functionality in suspension conditions. Advanced cell-material interactions, cell partial coatings, cell-based materials, organic-inorganic hybrid composites, sol-gel processes. [Extracted from the article]