Search

Novel, simple, accurate and reliable Reverse Phase High-Performance Thin-Layer Chromatographic (RPHPTLC) method for analysis of Agomelatine (AGM) in bulk and tablet formulation have been developed and validated. The method was reverse phase chromatography performed on RP-18 Silica gel F254S TLC plates, with methanol: water (80: 20 %, v/v) as mobile phase. HPTLC quantitation of AGM was done by TLC-densitometry at 276 nm. The quantitation by HPTLC method was performed over the concentration range of 500-3000 ng/band for AGM with a high correlation coefficient (R2 = 0.999). The HPTLC method resulted into a compact and well resolved band for AGM at retention factor (Rf) of 0.43 ± 0.02. The limit of detection and limit of quantification were found to be 5.85 ng and 17.73 ng for RP-HPTLC, respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.23 to 99.98%. The proposed method is applicable to routine analysis of AGM in bulk and tablet formulations. The proposed method was validated according to various ICH parameters like linearity, accuracy, precision, specificity, limits of detection and limits of quantification. [ABSTRACT FROM AUTHOR]

A simple, rapid, and precise RP-HPTLC method for analysis of Prednisolone acetate and Moxifloxacin hydrochloride in their eye drop dosage forms has been developed and validated. Chromatography was performed on silica gel 60 RP-18F254S plates with Methanol:Water:Triethylamine 7:3:0.3 (v/v) as mobile phase. Detection was performed at 274 nm. The Rfvalues for Prednisolone acetate and Moxifloxacin hydrochloride were found to be 0.50 ± 0.02 and 0.72 ± 0.02, respectively. Regression plots revealed good linear relationships in the concentration ranges 600–3600 ng/band for Prednisolone acetate and 300–1800 ng/band for Moxifloxacin hydrochloride. When accuracy was checked by conducting recovery studies the % recoveries were ranging from 100.97% to 102.03% (Prednisolone acetate) and 100.49% to 101.25% (Moxifloxacin hydrochloride), respectively. The amount of Prednisolone acetate and Moxifloxacin hydrochloride estimated in marketed formulation were 99.22% and 101.07%, respectively. [ABSTRACT FROM AUTHOR]

Lafutidine (LTD) is a histamine H2 receptor antagonist, with anti-ulcer and mucosal protective activity. A sensitive and selective stability-indicating reversed- phase high-performance thin-layer chromatography (RP-HPTLC)/densitometry method is suggested for the determination of LTD in the presence of its acid, alkaline, oxidative and dry heat degradation products. Stress testing of LTD was done according to the International Conference on Harmonization (ICH) guidelines in order to validate the stability-indicating power of the analytical procedures. Stress testing demonstrated that LTD underwent acid, alkaline, oxidative and dry heat degradation; on the other hand, it showed stability towards neutral and photo degradation. Chromatography was performed on aluminum-backed silica gel 60 RP-18 F254 S plates with methanol:water:triethylamine 8:2:0.5 (v/v) as the mobile phase. Densitometric scanning was performed at 276 nm. The system gave compact bands for LTD (Rf 0.67±0.02). Linear regression analysis data for the calibration plots showed a good linear relationship with r² > 0.99 in the working concentration range 500 to 3000 ng per band. The limits of detection and quantification were 23.51 ng and 71.26 ng, respectively. The developed method can routinely be used for analysis of LTD in bulk and pharmaceutical formulations. [ABSTRACT FROM AUTHOR]

Silica gel plates impregnated with a variety of oils including vegetable oils (olive, sunflower and corn oil) and synthetic oils (trioctylamine and paraffin oil) were evaluated and compared with the commercially available reversed-phases TLC plates (RP-18, RP-18W, and CN). A series of selected lipophilic vitamins was employed to evaluate the suitability of oils as reversed-phases for TLC and to provide different lipophilicity indices: R, scores corresponding to the first principal component of R and/or R the arithmetic mean of R and R values obtained with solvent mixture containing various concentrations of methanol in water. The retention results were excellent ( r > 0.98) and allowed accurate estimation of lipophilicity of selected vitamins and to ranking the lipophilicity of oils when comparing with chemically bonded phases. Concerning the lipophilicity scale of vegetable oils, it is worth noting that corn oil presents the highest lipophilicity, closely followed by the olive and sunflower oils. [ABSTRACT FROM AUTHOR]