1. Linking single-cell measurements of mass, growth rate, and gene expression.
- Author
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Kimmerling RJ, Prakadan SM, Gupta AJ, Calistri NL, Stevens MM, Olcum S, Cermak N, Drake RS, Pelton K, De Smet F, Ligon KL, Shalek AK, and Manalis SR
- Subjects
- Animals, CD8-Positive T-Lymphocytes metabolism, Cell Line, Tumor, Humans, Lymphocyte Activation, Mice, Cell Enlargement, Genomics methods, Microfluidic Analytical Techniques, Single-Cell Analysis methods
- Abstract
Mass and growth rate are highly integrative measures of cell physiology not discernable via genomic measurements. Here, we introduce a microfluidic platform enabling direct measurement of single-cell mass and growth rate upstream of highly multiplexed single-cell profiling such as single-cell RNA sequencing. We resolve transcriptional signatures associated with single-cell mass and growth rate in L1210 and FL5.12 cell lines and activated CD8+ T cells. Further, we demonstrate a framework using these linked measurements to characterize biophysical heterogeneity in a patient-derived glioblastoma cell line with and without drug treatment. Our results highlight the value of coupled phenotypic metrics in guiding single-cell genomics.
- Published
- 2018
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