13 results on '"Watt, Kathryn"'
Search Results
2. Associations between MHC class II variation and phenotypic traits in a free‐living sheep population.
- Author
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Huang, Wei, Dicks, Kara L., Ballingall, Keith T., Johnston, Susan E., Sparks, Alexandra M., Watt, Kathryn, Pilkington, Jill G., and Pemberton, Josephine M.
- Subjects
PHENOTYPIC plasticity ,SHEEP breeding ,MAJOR histocompatibility complex ,IMMUNOGLOBULIN G ,IMMUNOGLOBULIN E ,SHEEP - Abstract
Pathogen‐mediated selection (PMS) is thought to maintain the high level of allelic diversity observed in the major histocompatibility complex (MHC) class II genes. A comprehensive way to demonstrate contemporary selection is to examine associations between MHC variation and individual fitness. As individual fitness is hard to measure, many studies examine associations between MHC variation and phenotypic traits, including direct or indirect measures of adaptive immunity thought to contribute to fitness. Here, we tested associations between MHC class II variation and five phenotypic traits measured in free‐living sheep captured in August: weight, strongyle faecal egg count, and plasma IgA, IgE and IgG immunoglobulin titres against the gastrointestinal nematode parasite Teladorsagia circumcincta. We found no association between MHC class II variation and weight or strongyle faecal egg count. We did, however, find associations between MHC class II variation and immunoglobulin levels which varied with isotype, age and sex. Our results suggest associations between MHC and phenotypic traits are more likely to be found for traits more closely associated with pathogen defence than integrative traits such as bodyweight and highlight the association between MHC variation and antibodies in wild populations. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
3. Maternally derived anti-helminth antibodies predict offspring survival in a wild mammal.
- Author
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Sparks, Alexandra M., Hayward, Adam D., Watt, Kathryn, Pilkington, Jill G., Pemberton, Josephine M., Johnston, Susan E., McNeilly, Tom N., and Nussey, Daniel H.
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IMMUNOGLOBULINS ,ANTIBODY titer ,ANTIBODY formation ,MATERNAL age ,MOTHERS ,MATERNALLY acquired immunity ,HELMINTHIASIS ,SHEEP breeding - Abstract
The transfer of antibodies from mother to offspring provides crucial protection against infection to offspring during early life in humans and domestic and laboratory animals. However, few studies have tested the consequences of variation in maternal antibody transfer for offspring fitness in the wild. Further, separating the immunoprotective effects of antibodies from their association with nutritional resources provided by mothers is difficult. Here, we measured plasma levels of total and parasite-specific antibodies in neonatal (less than 10 days old) wild Soay sheep over 25 years to quantify variation in maternal antibody transfer and test its association with offspring survival. Maternal antibody transfer was predicted by maternal age and previous antibody responses, and was consistent within mothers across years. Neonatal total IgG antibody levels were positively related to early growth, suggesting they reflected nutritional transfer. Neonatal parasitespecific IgG levels positively predicted first-year survival, independent of lamb weight, total IgG levels and subsequent lamb parasite-specific antibody levels. This relationship was partly mediated via an indirect negative association with parasite burden. We show that among-female variation in maternal antibody transfer can have long-term effects on offspring growth, parasite burden and fitness in the wild, and is likely to impact naturally occurring host-parasite dynamics. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
4. Sex differences in leucocyte telomere length in a free-living mammal
- Author
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Watson, Rebecca L., Bird, Ellen J., Underwood, Sarah, Wilbourn, Rachael V., Fairlie, Jennifer, Watt, Kathryn, Salvo‐Chirnside, Eliane, Pilkington, Jill G., Pemberton, Josephine M., McNeilly, Tom N., Froy, Hannah, and Nussey, Daniel H.
- Subjects
Ovis aries ,Male ,Sex Characteristics ,Sheep ,Soay sheep ,lymphocyte ,Telomere ,Cross-Sectional Studies ,granulocyte ,Leukocytes ,sexual selection ,Animals ,Original Article ,Female ,ORIGINAL ARTICLES ,Telomere Shortening ,Ecological Genomics ,polygyny - Abstract
Mounting evidence suggests that average telomere length reflects previous stress and predicts subsequent survival across vertebrate species. In humans, leucocyte telomere length (LTL) is consistently shorter during adulthood in males than in females, although the causes of this sex difference and its generality to other mammals remain unknown. Here, we measured LTL in a cross‐sectional sample of free‐living Soay sheep and found shorter telomeres in males than in females in later adulthood (>3 years of age), but not in early life. This observation was not related to sex differences in growth or parasite burden, but we did find evidence for reduced LTL associated with increased horn growth in early life in males. Variation in LTL was independent of variation in the proportions of different leucocyte cell types, which are known to differ in telomere length. Our results provide the first evidence of sex differences in LTL from a wild mammal, but longitudinal studies are now required to determine whether telomere attrition rates or selective disappearance are responsible for these observed differences., see also the Perspective by Dantzer and Garratt
- Published
- 2016
5. Plasma markers of oxidative stress are uncorrelated in a wild mammal
- Author
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Christensen, Louise L., Selman, Colin, Blount, Jonathan D., Pilkington, Jill G., Watt, Kathryn A., Pemberton, Josephine M., Reid, Jane M., and Nussey, Daniel H.
- Subjects
Redox ,Plasma ,Soay sheep ,Ecology ,Oxidative damage ,Life history ,Reactive oxygen species ,Antioxidants ,Ecology, Evolution, Behavior and Systematics ,Nature and Landscape Conservation - Abstract
Oxidative stress, which results from an imbalance between the production of potentially damaging reactive oxygen species versus antioxidant defenses and repair mechanisms, has been proposed as an important mediator of life-history trade-offs. A plethora of biomarkers associated with oxidative stress exist, but few ecological studies have examined the relationships among different markers in organisms experiencing natural conditions or tested whether those relationships are stable across different environments and demographic groups. It is therefore not clear to what extent studies of different markers can be compared, or whether studies that focus on a single marker can draw general conclusions regarding oxidative stress. We measured widely used markers of oxidative damage (protein carbonyls and malondialdehyde) and antioxidant defense (superoxide dismutase and total antioxidant capacity) from 706 plasma samples collected over a 4-year period in a wild population of Soay sheep on St Kilda. We quantified the correlation structure among these four markers across the entire sample set and also within separate years, age groups (lambs and adults), and sexes. We found some moderately strong correlations between some pairs of markers when data from all 4 years were pooled. However, these correlations were caused by considerable among-year variation in mean marker values; correlation coefficients were small and not significantly different from zero after accounting for among-year variation. Furthermore, within each year, age, and sex subgroup, the pairwise correlation coefficients among the four markers were weak, nonsignificant, and distributed around zero. In addition, principal component analysis confirmed that the four markers represented four independent axes of variation. Our results suggest that plasma markers of oxidative stress may vary dramatically among years, presumably due to environmental conditions, and that this variation can induce population-level correlations among markers even in the absence of any correlations within contemporaneous subgroups. The absence of any consistent correlations within years or demographic subgroups implies that care must be taken when generalizing from observed relationships with oxidative stress markers, as each marker may reflect different and potentially uncoupled biochemical processes.
- Published
- 2015
6. Detecting genes for variation in parasite burden and immunological traits in a wild population: testing the candidate gene approach
- Author
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Brown, Emily A, Pilkington, Jill G, Nussey, Daniel H, Watt, Kathryn A, Hayward, Adam, Tucker, Rachel, Graham, Andrea, Paterson, Steve, Beraldi, Dario, Pemberton, Josephine M, and Slate, Jon
- Subjects
Soay sheep ,ecological immunity ,genomics ,candidate gene ,association study ,parasite resistance - Abstract
Identifying the genes underlying phenotypic variation in natural populations can provide novel insight into the evolutionary process. The candidate gene approach has been applied to studies of a number of traits in various species, in an attempt to elucidate their genetic basis. Here, we test the application of the candidate gene approach to identify the loci involved in variation in gastrointestinal parasite burden, a complex trait likely to be controlled by many loci, in a wild population of Soay sheep. A comprehensive literature review, Gene Ontology databases, and comparative genomics resources between cattle and sheep were used to generate a list of candidate genes. In a pilot study, these candidates, along with 50 random genes, were then sequenced in two pools of Soay sheep; one with low gastrointestinal nematode burden and the other high, using a NimbleGen sequence capture experiment. Further candidates were identified from single nucleotide polymorphisms (SNPs) that were highly differentiated between high- and low-resistance sheep breeds. A panel of 192 candidate and control SNPs were then typed in 960 individual Soay sheep to examine whether they individually explained variation in parasite burden, as measured as faecal egg count, as well as two immune measures (Teladorsagia circumcincta-specific antibodies and antinuclear antibodies). The cumulative effect of the candidate and control SNPs were estimated by fitting genetic relationship matrices (GRMs) as random effects in animal models of the three traits. No more significant SNPs were identified in the pilot sequencing experiment and association study than expected by chance. Furthermore, no significant difference was found between the proportions of candidate or control SNPs that were found to be significantly associated with parasite burden/immune measures. No significant effect of the candidate or control gene GRMs was found. There is thus little support for the candidate gene approach to the identification of loci explaining variation in parasitological and immunological traits in this population. However, a number of SNPs explained significant variation in multiple traits and significant correlations were found between the proportions of variance explained by individual SNPs across multiple traits. The significant SNPs identified in this study may still, therefore, merit further investigation.
- Published
- 2013
7. Sex differences in leucocyte telomere length in a free-living mammal.
- Author
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Watson, Rebecca L., Bird, Ellen J., Underwood, Sarah, Wilbourn, Rachael V., Fairlie, Jennifer, Watt, Kathryn, Salvo‐Chirnside, Eliane, Pilkington, Jill G., Pemberton, Josephine M., McNeilly, Tom N., Froy, Hannah, and Nussey, Daniel H.
- Subjects
SOAY sheep ,LEUCOCYTES ,TELOMERES ,SEXUAL selection - Abstract
Mounting evidence suggests that average telomere length reflects previous stress and predicts subsequent survival across vertebrate species. In humans, leucocyte telomere length ( LTL) is consistently shorter during adulthood in males than in females, although the causes of this sex difference and its generality to other mammals remain unknown. Here, we measured LTL in a cross-sectional sample of free-living Soay sheep and found shorter telomeres in males than in females in later adulthood (>3 years of age), but not in early life. This observation was not related to sex differences in growth or parasite burden, but we did find evidence for reduced LTL associated with increased horn growth in early life in males. Variation in LTL was independent of variation in the proportions of different leucocyte cell types, which are known to differ in telomere length. Our results provide the first evidence of sex differences in LTL from a wild mammal, but longitudinal studies are now required to determine whether telomere attrition rates or selective disappearance are responsible for these observed differences. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
8. Marker-dependent associations among oxidative stress, growth and survival during early life in a wild mammal.
- Author
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Christensen, Louise L., Selman, Colin, Blount, Jonathan D., Pilkington, Jill G., Watt, Kathryn A., Pemberton, Josephine M., Reid, Jane M., and Nussey, Daniel H.
- Subjects
ANTIOXIDANTS ,OXIDATIVE stress ,MAMMAL growth ,SPATIAL variation ,SOAY sheep - Abstract
Oxidative stress (OS) is hypothesized to be a key physiologicalmechanismmediating life-history trade-offs, but evidence from wild populations experiencing natural environmental variation is limited. We tested the hypotheses that increased early life growth rate increases OS, and that increased OS reduces first-winter survival, in wild Soay sheep (Ovis aries) lambs. We measured growth rate andfirst-winter survival for four consecutive cohorts, andmeasured twomarkers of oxidative damage (malondialdehyde (MDA), protein carbonyls (PC)) and two markers of antioxidant (AOX) protection (total AOX capacity (TAC), superoxide dismutase (SOD)) from blood samples. Faster lamb growth was weakly associated with increased MDA, but not associated with variation in the other three markers. Lambs with higher SOD activity were more likely to survive their first winter, as were male but not female lambs with lower PC concentrations. Survival did not vary with MDA or total TAC. Key predictions relating OS to growth and survival were therefore supported in some OS markers, but not others. This suggests that different markers capture different aspects of the complex relationships between individual oxidative state, physiology and fitness, and that overarching hypotheses relating OS to life-history variation cannot be supported or refuted by studying individual markers. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
9. Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
- Author
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Watt, Kathryn A., Nussey, Daniel H., Maclellan, Rachel, Pilkington, Jill G., and McNeilly, Tom N.
- Subjects
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NEMATODES , *GASTROINTESTINAL system , *IMMUNOGLOBULINS , *ANTIGENS , *RUMINANTS - Abstract
Among-individual variation in antibody-associated immunity to gastrointestinal nematode parasites ( GIN) is known be associated with life-history traits and vital rates in wild vertebrate systems. To date, measurement of levels of antibodies against GIN antigens in natural populations has exclusively been based on invasive blood sampling techniques. Previous work in laboratory rodents and ruminant livestock suggests that antibody measures from feces may provide a viable noninvasive approach. We measured total and anti- GIN antibodies of different isotypes (immunoglobulin (Ig) G, IgA and IgE) from paired samples of plasma and feces from free-living Soay sheep of different ages and sexes. We tested the correlations among these measures as well as their associations with body mass and Strongyle nematode fecal egg counts ( FEC). Significant positive correlations were present among plasma and fecal anti- GIN antibody levels for IgG and IgA. Generally, correlations between total antibody levels in plasma and feces were weaker and not significant. No significant relationships were found between any antibody measures and body mass; however, fecal anti- GIN antibody levels were significantly negatively correlated with FEC. Our data clearly demonstrate the feasibility of measuring anti- GIN antibodies from fecal samples collected in natural populations. Although associations of fecal antibody levels with their plasma counterparts and FEC were relatively weak, the presence of significant correlations in the predicted direction in a relatively small and heterogeneous sample suggests fecal antibody measures could be a useful, noninvasive addition to current eco-immunological studies. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
10. Osteoarthritis of the temporo-mandibular joint in free-living Soay sheep on St Kilda.
- Author
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Arthur, Colin, Watt, Kathryn, Nussey, Daniel H., Pemberton, Josephine M., Pilkington, Jill G., Herman, Jeremy S., Timmons, Zena L., Clements, Dylan N., and Scott, Philip R.
- Subjects
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OSTEOARTHRITIS , *ARTHRITIS , *HALLUX rigidus , *SOAY sheep , *SHEEP breeds - Abstract
Osteoarthritis (OA) is a common degenerative disease of synovial joints with the potential to cause pathology and welfare issues in both domestic and wild ruminants. Previous work has identified OA of the elbow joint in domestic sheep, but the prevalence of OA of the jaw and in particular the temporomandibular joint (TMJ) has not been previously reported. Following up a previous report of a single case of TMJ OA in a free-living population of Soay sheep on St Kilda in the Outer Hebrides, an archive of 2736 jaw bones collected from this population between 1985 and 2010 was surveyed. Evidence of TMJ OA was found in 35 sheep. Of these, 15 cases were unilateral (11 right side, 4 left side) and the remaining 20 were bilateral. TMJ pathology was much more common in females than males: only 3/35 cases were in males, with overall prevalence at 2.3% for females and 0.2% in males. Radiographic examination of TMJ with OA revealed extensive bone re-modelling with osteophytosis, particularly of the condyle of the mandible. There was a highly significant age-dependence in TMJ OA incidence among age classes: 30/35 cases occurred in geriatric sheep (aged 7 years or more; 11.1% prevalence within this age class), four in adults (2-6 years old; 0.9% prevalence), one in yearlings (0.3% prevalence) and none in lambs. The low incidence in males was confounded by sex differences in longevity: while 18% of females sampled died in the geriatric age class, only 2% of males did so. Although the low prevalence of the pathology limited the ability to test its association with other traits, it was possible to examine relationships with reproductive performance measures amongst geriatric females w ith and w ithout TMJ OA. Although there were trends towards lower fecundity and lower lamb birth weight in the breeding season prior to death, these were not statistically significant. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
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11. Multivariate immune defences and fitness in the wild: complex but ecologically important associations among plasma antibodies, health and survival.
- Author
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Nussey, Daniel H., Watt, Kathryn A., Clark, Abigail, Pilkington, Jill G., Pemberton, Josephine M., Graham, Andrea L., and McNeilly, Tom N.
- Subjects
- *
PLASMA cells , *IMMUNE system , *IMMUNOGLOBULINS , *SOAY sheep , *BIOLOGICAL evolution - Abstract
Despite our rapidly advancing mechanistic understanding of vertebrate immunity under controlled laboratory conditions, the links between immunity, infection and fitness under natural conditions remain poorly understood. Antibodies are central to acquired immune responses, and antibody levels circulating in vivo reflect a composite of constitutive and induced functional variants of diverse specificities (e.g. binding antigens from prevalent parasites, self tissues or novel non-self sources). Here, we measured plasma concentrations of 11 different antibody types in adult females from an unmanaged population of Soay sheep on St Kilda. Correlations among antibody measures were generally positive butweak, and eight of the measures independently predicted body mass, strongyle parasite egg count or survival over the subsequent winter. These independent and, in some cases, antagonistic relationships point to important multivariate immunological heterogeneities affecting organismal health and fitness in natural systems. Notably, we identified a strong positive association between anti-nematode immunoglobulin (Ig) G antibodies in summer and subsequent over-winter survival, providing rare evidence for a fitness benefit of helminth-specific immunity under natural conditions. Our results highlight both the evolutionary and ecological importance and the complex nature of the immune phenotype in the wild. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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12. Fitness Correlates of Heritable Variation in Antibody Responsiveness in a Wild Mammal.
- Author
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Graham, Andrea L., Hayward, Adam D., Watt, Kathryn A., Pilkington, Jill G., Pemberton, Josephine M., and Nussey, Daniel H.
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SOAY sheep , *MAMMALOGICAL research , *PHYSICAL fitness research , *IMMUNE response , *AUTOIMMUNE diseases , *HEREDITY , *IMMUNOGLOBULINS , *NATURAL selection , *STATISTICAL correlation - Abstract
A functional immune system is important for survival in natural environments, where individuals are frequently exposed to parasites. Yet strong immune responses may have fitness costs if they deplete limited energetic resources or cause autoimmune disease. We have found associations between fitness and heritable self-reactive antibody responsiveness in a wild population of Soay sheep. The occurrence of self-reactive antibodies correlated with overall antibody responsiveness and was associated with reduced reproduction in adults of both sexes. However, in females, the presence of self-reactive antibodies was positively associated with adult survival during harsh winters. Our results highlight the complex effects of natural selection on immune responsiveness and suggest that fitness trade-offs may maintain immunoheterogeneity, including genetic variation in autoimmune susceptibility. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
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13. Gastrointestinal nematode species diversity in Soay sheep kept in a natural environment without active parasite control.
- Author
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Sinclair, Rona, Melville, Lynsey, Sargison, Fiona, Kenyon, Fiona, Nussey, Dan, Watt, Kathryn, and Sargison, Neil
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- *
NEMATODE infections , *SOAY sheep , *NATURE , *SEQUENCE analysis , *HAEMONCHUS contortus , *PREVENTION - Abstract
Molecular methods based on ITS2 sequence analysis were used to identify strongylid parasites and describe their diversity in a management intervention and anthelmintic drug treatment-free sheep flock. Fourteen different nematode parasite species were identified in the flock and the results showed a greater level of nematode species diversity than is normally reported in managed farmed flocks, with the presence of parasites such as Bunostomum trigonocephalum, Ostertagia leptospicularis , Spiculopteragia houdemeri and Trichostrongylus retortaeformis that are considered to be absent or rare in sheep kept in comparable localities. The implied prevalences of Haemonchus contortus in lambs, and of Trichostrongylus axei in lambs, ewes and rams, were higher than those in farmed sheep kept in similar regions, while those of Teladorsagia circumcincta and Trichostrongylus vitrinus were lower. Comparison of the patterns of nematode parasite infection between the summer and autumn sampling periods showed differences from the scenarios that are commonplace in comparable managed flocks; with T. vitrinus burdens of the lambs being higher in the summer than in the winter, and Oesophagostomum venulosum being the predominant nematode species in the adult sheep during the summer, while more-or-less absent from these groups during the winter. Rams played an important role in the epidemiology of certain parasitic nematode species. The relatively non-pathogenic O. venulosum was the only parasitic nematode species to predominate in any group during the study. This preliminary characterisation of the nematode parasite burdens of sheep extensively grazed on diverse unimproved pastures will aid in the understanding of the parasitological consequences of intensive grazing management and of the manner in which modern agriculture upsets the equilibrium between parasites and their hosts. These factors must be accounted for when defining the concept of sustainable parasite control and informing sustainability with reference to commercially efficient sheep farming. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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