The article focuses on an experiment that use Prisoner's Dilemma game. Recent experiments using the Prisoner's Dilemma (PD) have been equivocal with regard to how variations in the strategy of the other player affect the development of mutual cooperation. In an experiment the effects of two simulated strategies were compared in which there were systematic variations in the number and sequence of cooperative runs. The first strategy progressed through 40 trials with 95 percent cooperation, 20 trials with 50 percent cooperation, 80 trials with five percent cooperation, 20 trials with 50 percent cooperation, and 40 trials of 95 percent cooperation. The second strategy, while retaining the same sequence of runs, inverted the order of cooperation (5, 50, 95, 50, and 5 percent). The purpose was to determine in what way the changes in progressions from descending to ascending levels of cooperation would affect the subjects' game behavior. The results indicated that the ascending levels of cooperation (5, 50, 95 percent) elicited a significant increase in the simulated strategies level of cooperative response.