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The bioremediation of polycyclic aromatic hydrocarbon (PAHs) from soil utilizing microorganisms, enzymes, microbial consortiums, strains, etc. has attracted a lot of interest due to the environmentally friendly, and cost-effective features. Enzymes can efficiently break down PAHs in soil by hydroxylating the benzene ring, breaking the C-C bond, and catalyze the hydroxylation of a variety of benzene ring compounds via single-electron transfer oxidation. However, the practical application is limited by its instability and ease to loss function under harsh environmental conditions such as pH, temperature, and edaphic stress etc. Therefore, this paper focused on the techniques used to immobilize enzymes and remediate PAHs in soil. Moreover, previous research has not adequately covered this topic, despite the employment of several immobilized enzymes in aqueous solution cultures to remediate other types of organic pollutants. Bibliometric analysis further highlighted the research trends from 2000 to 2023 on this field of growing interest and identified important challenges regarding enzyme stability and interaction with soil matrices. The findings indicated that immobilized enzymes may catalyzed PAHs via oxidation of OH groups in benzene rings, and generate benzyl radicals (i.e., • OH and • O 2 ) that undergo further reaction and release water. As a result, the intermediate products of PAHs further catalyze by enzyme and enzyme induced microbes producing carbon dioxide and water. Meanwhile efficiency, activity, lifetime, resilience, and sustainability of immobilized enzyme need to be further improved for the large-scale and field-scale clean-up of PAHs polluted soils. This could be possible by integrating enzyme-based with microbial and plant-based remediation strategies. It can be coupled with another line of research focused on using a new set of support materials that can be derived from natural resources., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

The impact of functionality of biochar on pressing environmental issue of cadmium (Cd) and lead (Pb) co-contamination in simultaneous soil and water systems has not sufficiently reported. This study investigated the impact of Fe- and Mg-functionalized wheat straw biochar (Fe-WSBC and Mg-WSBC) on Cd and Pb adsorption/immobilization through batch sorption and column leaching trials. Importantly, Fe-WSBC was more effective in adsorbing Cd and Pb (82.84 and 111.24 mg g -1 ), regeneration ability (removal efficiency 94.32 and 92.365), and competitive ability under competing cations (83.15 and 84.36%) compared to other materials (WSBC and Mg-WSBC). The practical feasibility of Fe-WSBC for spiked river water verified the 92.57% removal of Cd and 85.73% for Pb in 50 mg L -1 and 100 mg L -1 contamination, respectively. Besides, the leaching of Cd and Pb with Fe-WSBC under flow-through conditions was lowered to (0.326 and 17.62 mg L -1 ), respectively as compared to control (CK) (0.836 and 40.40 mg L -1 ). In short, this study presents the applicable approach for simultaneous remediation of contaminated water and soil matrices, offering insights into environmentally friendly green remediation strategies for heavy metals co-contaminated matrices., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Foliar application of zinc (Zn) or silicon nanoparticles (Si-NPs) may exert regulatory effects on cadmium (Cd) accumulation in rice grains, however, their impact on Cd bioavailability during human rice consumption remains elusive. This study comprehensively investigated the application of Zn with or without Si-NPs in reducing Cd accumulation in rice grains as well to exactly evaluate the potential risk of Cd exposure resulting from the rice consumption by employing field experiment as well laboratory bioaccessibility and bioavailability assay. Sole Zn (ZnSO 4 ) or in combination with Si (ZnSO 4 +Si and ZnO+Si) efficiently lowered the Cd concentration in rice grains. However, the impact of bioaccessible (0.1215-0.1623 mg kg -1 ) and bioavailable Cd (0.0245-0.0393 mg kg -1 ) during simulated human rice consumption depicted inconsistent trend. The straw HCl-extractable fraction of Cd (F HCl-Cd ) exhibited a significant correlation with total, bioaccessible, and bioavailable Cd in grains, indicating the critical role of F HCl-Cd in Cd accumulation and translocation from grains to human. Additionally, foliar spraying of Zn+Si raised the nutritional value of rice grains, leading to increased protein content and reduced phytic acid concentration. Overall, this study demonstrates the potential of foliar application of ZnSO 4 +Si in mitigating the Cd levels in rice grains and associated health risks upon consumption., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Abiogenic silicon (Si), though deemed a quasi-nutrient, remains largely inaccessible to plants due to its prevalence within mineral ores. Nevertheless, the influence of Si extends across a spectrum of pivotal plant processes. Si emerges as a versatile boon for plants, conferring a plethora of advantages. Notably, it engenders substantial enhancements in biomass, yield, and overall plant developmental attributes. Beyond these effects, Si augments the activities of vital antioxidant enzymes, encompassing glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD), among others. It achieves through the augmentation of reactive oxygen species (ROS) scavenging gene expression, thus curbing the injurious impact of free radicals. In addition to its effects on plants, Si profoundly ameliorates soil health indicators. Si tangibly enhances soil vitality by elevating soil pH and fostering microbial community proliferation. Furthermore, it exerts inhibitory control over ions that could inflict harm upon delicate plant cells. During interactions within the soil matrix, Si readily forms complexes with potentially toxic metals (PTEs), encapsulating them through Si-PTEs interactions, precipitative mechanisms, and integration within colloidal Si and mineral strata. The amalgamation of Si with other soil amendments, such as biochar, nanoparticles, zeolites, and composts, extends its capacity to thwart PTEs. This synergistic approach enhances soil organic matter content and bolsters overall soil quality parameters. The utilization of Si-based fertilizers and nanomaterials holds promise for further increasing food production and fortifying global food security. Besides, gaps in our scientific discourse persist concerning Si speciation and fractionation within soils, as well as its intricate interplay with PTEs. Nonetheless, future investigations must delve into the precise functions of abiogenic Si within the physiological and biochemical realms of both soil and plants, especially at the critical juncture of the soil-plant interface. This review seeks to comprehensively address the multifaceted roles of Si in plant and soil systems during interactions with PTEs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Humans are mainly exposed to cadmium (Cd) due to the rice consumption, however there exist considerable differences across rice cultivars in terms of Cd absorption and accumulation in the grains, and subsequent release after digestion (bioaccessibility), as well as uptake by Caco-2 cells of humans (bioavailability). This study comprised of field and lab simulation trials where in the field, firstly 39 mid-rice cultivars were screened for their phytoremediation potential coupled with safe production in relation to uptake and translocation of Cd. Lower Cd concentrations (˂0.2 mg kg -1 ) in polished rice of 74 % cultivars were ascribed to the increased root to straw translocation indicating that straw may acquire higher accumulation of Cd. Furthermore, the ionomic profile demonstrated that the spatial distribution of metals in different rice organs corresponds to the plant growth morphology. In the second year, in vitro-in vivo assay model was employed to assess the bioaccessibility and bioavailability of Cd in polished rice and to further estimate the daily Cd intake by humans through rice grains. The results of bioaccessibility and bioavailability assays and daily estimated Cd intake presented the corresponding values of 39.02-59.76 %, 8.69-24.26 %, and 0.0185-0.9713 μg kg -1 body weight day -1 , respectively. There exists a strong connection between total Cd and bioaccessible Cd to humans (R 2 = 0.94, P < 0.01). Polynomial fitting (R 2 = 0.91, P < 0.01) showed a better statistically significant correlation between total Cd contents and bioavailable levels, suggesting that in vitro-in vivo assays should be considered in future studies. The results of field experiments and in vitro-in vivo assays recommended the Tianyouhuazhan (MR-29), Heliangyou1hao (MR-17), and Yongyou15 (MR-1) as suitable mid-rice cultivars for the phytoremediation of slightly Cd contaminated soils coupled with rice agro-production due to their high nutritional value and low total and bioavailable Cd for human., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

In the present study, we have underpinned the serpentine rock, serpentinized ultramafic soil and rhizosphere's microbial communities, signifying their heavy metals-exposed taxa signatures and functional repertoires in comparison to non-serpentine soils. The results revealed that the serpentine rock embedded soil highlighted the geo-accumulation of higher amount of Cr and Ni impacting soil microbial diversity negatively by metal stress-driven selection. Biolog Ecoplate CLPP defined a restricted spectrum of C-utilization in the higher heavy metal-containing serpentine samples compared to non-serpentine. The linear discriminant analysis (LDA) score identified a higher abundance of Desulfobacterota, Opitutales, and Bacteroidales in low Cr and Ni-stressed non-serpentine-exposed samples. Whereas the abundance of Propionibacteriales and Actinobacteriota were significantly enriched in the serpentine niche. Further, the C, N, S, Fe, and methane biogeochemical cycles linked functional members were identified, and showing higher functional diversity in low Cr and Ni concentration-containing rhizosphere JS-soils. The Pearson correlation coefficient (r) value confirmed the abundance of functional members linked to specific biogeochemical cycle, positively correlated with relevant pathway enrichment. Ultimately, this study highlighted the heavy metal stress within a serpentine setting that could limit the resident microbial community's metabolic diversity and further select the bacteria that could thrive in the serpentine-associated heavy metal-stressed soils. These acclimatized microbes could pave the way for the future applications in the soil conservation and management., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Heavy metal release from harsh ultramafic settings influences microbial diversity and function in soil ecology. This study aimed to determine how serpentine mineralosphere bacterial assemblies and their functions differed in two different plate-tectonic plains and mining exposure sites under heavy metal release conditions. The results showed that the Proteobacteria, Actinobacteria, Cyanobacteria, Planctomycetes, and Chloroflexi were the most abundant bacterial groups among all the sites. The log10-based LDA scores highlighted that some specific groups of bacterial assemblies were enriched in plate-tectonic plains and mining activity areas of the serpentine mineralosphere. Functional prediction revealed that the abundance of heavy metal (Cr and Ni) resistance and biogeochemical cycles involving functional KEGG orthology varied in samples from plate-tectonic plains and mining activity sites. The bipartite plot showed that the enrichment of the biogeochemical cycle and heavy metal resistance functional genes correlated with the abundance of serpentine mineralosphere bacterial groups at a 0.005% confidence level. The co-occurrence network plot revealed that the interconnection pattern of the indigenous bacterial assemblies changed in different plate-tectonic plains and mining exposure areas. Finally, this study concluded that due to heavy metal release, the variation in bacterial assemblies, their functioning, and intercommunity co-occurrence patterns were clarified the synergetic effect of mineral-microbial geochemical weathering process in serpentine mining areas., (Copyright © 2022. Published by Elsevier Inc.)

Previous studies have focused on total cadmium (Cd) accumulation in rice or its transformation in soil, but only a few have examined the entire soil-rice-human system. This study investigated the Cd bioaccessibility and bioavailability for humans from grains of early rice cultivars grown in a Cd-polluted field and further combined with multi-traits to discover and evaluate the optimum safe production and phytoremediation potential cultivars. The results revealed that Cd concentration in polished rice was <0.20 mg kg -1 in 79 % of early rice cultivars, implying that Cd levels in rice might be reduced by cultivar selection. Furthermore, the higher values of root to straw translocation factor indicates the maximal accumulation of Cd in straw and with highest soil to straw accumulation factor (>1.0) in 66.67 % of cultivars. However, bioaccessibility and bioavailability varied greatly among cultivars with corresponding values ranging from 5.68 to 7.67 % and 1.87 to 5.71 ng g -1 , respectively. Despite the fact that polynomial fitting revealed a statistically significant relationship between Cd content in polished rice and bioavailable Cd in humans (R 2 = 0.718, P = 0.025), poor goodness of fit for bioaccessibility, bioavailability, and toxicity varied even within low-Cd accumulating cultivars. As a result of multi trait analysis and bioavailability, Zhuliangyou4024 (ER-9), Lingliangyou211 (ER-3), and Yonxian15 (ER-28) were found to be the three best early rice cultivars with higher essential nutrients, less total and bioavailable Cd, and relative high phytoremediation potential and are suitable for healthy rice production and soil remediation., Competing Interests: Declaration of competing interest All authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Environmental pollution of heavy metals (HMs), mainly due to anthropogenic activities, has received growing attention in recent decades. HMs, especially the non-essential carcinogenic ones, including chromium (Cr), cadmium (Cd), mercury (Hg), aluminum (Al), lead (Pb), and arsenic (As), have appeared as the most significant air, water, and soil pollutants, which adversely affect the quantity, quality, and security of plant-based food all over the world. Plants exposed to HMs could experience significant decline in growth and yield. To avoid or tolerate the toxic effects of HMs, plants have developed complicated defense mechanisms, including absorption and accumulation of HMs in cell organelles, immobilization by forming complexes with organic chelates, extraction by using numerous transporters, ion channels, signalling cascades, and transcription elements, among others. OMICS strategies have developed significantly to understand the mechanisms of plant transcriptomics, genomics, proteomics, metabolomics, and ionomics to counter HM-mediated stress stimuli. These strategies have been considered to be reliable and feasible for investigating the roles of genomics (genomes), transcriptomic (coding), mRNA transcripts (non-coding), metabolomics (metabolites), and ionomics (metal ions) to enhance stress resistance or tolerance in plants. The recent developments in the mechanistic understandings of the HMs-plant interaction in terms of their absorption, translocation, and toxicity invasions at the molecular and cellular levels, as well as plants' response and adaptation strategies against these stressors, are summarized in the present review. Transcriptomics, genomics, metabolomics, proteomics, and ionomics for plants against HMs toxicities are reviewed, while challenges and future recommendations are also discussed., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

This study aims to determine and compare the bacterial community and functional profiles associated with serpentine sites, innate hyper-accumulating weed, downgradient agricultural farmlands and non-serpentine sites using 16S rRNA gene sequencing. Elemental analysis revealed that the serpentine rock and weathered soil have higher magnesium, nickel, chromium, magnesium/calcium and lower calcium/magnesium ratios and agricultural farmlands have recorded elevated chromium. Proteobacteria were found predominant, except the non-serpentine site which was rich in Cyanobacteria. PCA analysis at the genus level indicates the uniqueness of different experimental groups, except the hyperaccumulators which exhibited relatively less dissimilarity. The shift analysis showed the serpentine sites were characterized by the abundance of bacteria having heavy metal effluxion. The hyper-accumulating weeds were higher in plant growth-promoting bacteria expressing tolerance against heavy metals toxicity such as nickel, chromium, cobalt and arsenic. Besides, the agricultural lands were abundant in wetland-associated methanogens and metal (manganese, iron and zinc) transporting function related bacteria. The results suggest that the inherent edaphic factors including heavy metal content, the interacting behavior of hyperaccumulator's rhizosphere microbiota with soil and anthropogenic activities such as agricultural practices could be a major determinant of the variation in the bacterial community selection and abundance in the respective study sites., (Copyright © 2022 Elsevier B.V. All rights reserved.)

This study explores the toxic effect of TCE at different depths of sub-surface soil and underpins microbial community-level suitable carbon (C)-sources that provided directionality to the in situ biostimulation effort via augmentation strategy for effective TCE remediation in soil. The impacts on resident microbial communities and their functional profiles that govern the TCE biodegradation process were identified. Highly contaminated PW01 soil (9 m depth) had severely limited microbial diversity and was enriched in Proteobacteria and Firmicutes. The abundance of TCE degradation-associated genera was observed in all contaminated samples, and the abundance of TCE-degradation-related taxa were positively correlated with soil TCE contamination levels. Community-level metabolic activity associated with the utilization of diverse external C-sources was directly influenced by TCE concentration and soil depth. Multivariate data analysis revealed that the functional genus, TCE concentration, and selected available C substrate uptake capacity correlated in soil samples. Pearson's correlation tests revealed that C sources such as L-arginine, phenylethylamine and γ-hydroxybutyric acid utilization trait exhibited significant positive correlations with chloroalkane and chloroalkene degradation pathway abundance. Ultimately, depth and TCE contamination level-associated soil microbiota and their most preferred C-source understanding could add to facilitate effective biostimulation via external nutrient amendment for efficient in situ TCE degradation., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Climate-related flooding poses a potential challenge to phytoremediation of metal polluted areas. In the present study, uptake, translocation and accumulation of heavy metals (Cd, Zn and Cu), and their phytoavailability in six flood-tolerant Salix genotypes were investigated under simulated seasonal flooding conditions (non-flooding conditions were kept as the control). Plants were cultivated in a greenhouse with open windows using the soil polluted with Cd, Zn and Cu for 110 days. All the control (non-flooded) genotypes did not exhibit visible toxic symptoms, whereas the flooded genotypes showed leaf chlorosis and developed both lenticels and adventitious roots. Biomass production and metal accumulation in tissues varied with Salix genotypes. The flooded genotypes dramatically decreased aerial biomass production compared with corresponding non-flooded genotypes. All the control Salix genotypes showed relatively high accumulation for Cd, Zn and Cu in aerial parts due to high EDTA-extractable metals in the rhizosphere, exhibiting phytoextraction features. In contrast, the flooded genotypes drastically decreased uptake, translocation, accumulation, and extraction capacities for Cd, Zn and Cu in aerial parts, differing with genotypes, and tended to phyto-stabilize them in roots, especially Cu. This study indicated that flooding is a leading factor on phytoremediation efficiency for contaminated sites with willows., (Copyright © 2021. Published by Elsevier Ltd.)

Cadmium (Cd) pollution in cultivated soils has posed a great risk to human health through the soil-plant-human pathway. Therefore, it is important to derive soil thresholds for the low-Cd accumulating genotype of wheat (Triticum aestivum L.) to promote its application in agricultural production on Cd-contaminated sites. Here, a pot experiment was performed to explore the transfer characteristics of Cd in two contrasting wheat genotypes at three different soils and the effect of soil parameters together with soil safety Cd thresholds derivation. Generally, grain Cd highly accumulating wheat genotype (Zhenmai10, HT) showed higher Cd accumulation in grains than grain Cd weakly accumulating wheat genotype (Aikang58, LT). Stepwise multiple linear regression (SMLR) analysis (log-transformed Freundlich-type) indicated that Cd accumulation in wheat grains was strongly related to soil total Cd concentration and pH for both genotypes (R 2 = 0.907*** for HT; R 2 = 0.910*** for LT). Combining the simple regression model of soil-plant transfer system with the risk assessment method based on human health, soil total Cd thresholds for three soils were calculated with the values of 0.62, 0.82, and 0.62 mg kg -1 in LT genotype and 0.31, 0.77, and 0.49 mg kg -1 in HT genotype. Therefore, we suggested that when deducing soil thresholds, the ability of wheat genotypes to accumulate Cd and soil properties should be considered because of the large differences in soil thresholds between different genotypes and types of soils. We believe our results will promote the application of low-Cd wheat genotypes to agricultural production, thereby ensuring the safety of their products., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Cadmium (Cd) toxicity in paddy soil and accumulation in rice plants and grains have got global concern due to its health effects. This review highlights the effects of soil factors including soil organic matter, soil pH, redox potential, and soil microbes which influencing Cd uptake by rice plant. Therefore, a comprehensive review of innovative and environmentally friendly management practices for managing Cd stress in rice is lacking. Thus, this review discusses the effect of Cd toxicity in rice and describes management strategies to offset its effects. Moreover, future research thrusts to reduce its uptake by rice has also been highlighted. Through phytoremediation, Cd may be extracted and stabilized in the soil while through microbes Cd can be sequestrated inside the microbial bodies. Increased Cd uptake in hyperaccumulator plants to remediate and convert the toxic form of Cd into non-toxic forms. While in chemical remediation, Cd can be washed out, immobilized and stabilized in the soil through chemical amendments. The organic amendments may help through an increase in soil pH, adsorption in its functional groups, the formation of complexations, and the conversion of exchangeable to residual forms. Developing rice genotypes with restricted Cd uptake and reduced accumulation in grain through conventional and marker-assisted breeding are fundamental keys for safe rice production. In this regard, the use of molecular techniques including identification of QTLs, CRISPR/Cas9, and functional genomics may be quite helpful., Competing Interests: Declaration of competing interest The authors declare no conflicts of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)

As arable land has become an important sink for cadmium (Cd), soil is being recognized as a major source of metals to the food chain. It becomes, therefore, essential to investigate metal mobility in contaminated soils and to identify suitable remediation strategies. For this, immobilization of Cd was evaluated in contaminated stagnic anthrosol: S1, gleysol: S2 and fluvisol: S3 under flow through conditions. Ten treatments including control were tested alone or in composite form firstly at natural Cd contents (0.58-0.69 mg kg -1 ). Here, T2 (lime), T5 (biochar) and T10 (composite amendment) were found better in reducing the Cd concentration in the soils' leachates, so, their efficacy was further investigated in the same soils of higher Cd contents (1 and 2 mg kg -1 imposed by soil spiking). Amendments significantly reduced the leachate metal contents especially in 1 mg kg -1 spiked soils. Characterization of T2, T5 and T10 revealed their structural transformations in all the studied soil types, while active functional groups e.g. C-O, CO, O-H, Si-O-Si, ester and alcoholic groups were notably involved in Cd precipitation or adsorption on amendments surface. Variations in Cd speciation in these soils exhibited the exchange of Cd to more stable fractions with tested amendments. These continuous-flow experiments confirmed the strong efficiency of T2, T5 and T10 in reducing the Cd concentration in the leachate of three soils. This study has strong implications in understanding the role of different amendments in controlling the fate, leaching behavior and immobilization of Cd in diverse soil types., Competing Interests: Declaration of competing interest Authors declare no conflict of interest., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Cadmium (Cd) is a toxic trace metal that has polluted 20% of agricultural land in China where its concentration exceeds the standards for Chinese farmland. Plants are capable of accumulating Cd and other trace metals, but this capacity varies with species and cultivars within a species. Rice is a staple food consumed by half of the global population. In order to select safe late rice cultivars that are suitable late rice cultivars that can be cultivated in for growing in slightly contaminated soil, a two-year field experiment was conducted with 27 in the first year and 9 late rice cultivars in the second year. The results showed that plant Cd concentrations varied among the cultivars, with high magnitudes of variation occurred in straw and grains. Five genotypes including LR-12, LR-17, LR-24, LR-25 and LR-26 were identified as low accumulators for the first year while LR-15 and LR-17 were identified as promising cultivars based on Cd concentration in the polished rice grains (<0.02 mg kg -1 DW). In addition, these cultivars had favorable traits, including mineral nutrition and grain yield. Therefore, these genotypes should be considered for cultivation in slightly or moderately Cd contaminated soils.

The present study evaluated the efficiency of pre-selected composite amendments (CA-1: biochar-lime-sepiolite-zeolite and CA-2: manure-lime-sepiolite) for immobilization and sorption of Cd and Pb in field and batch sorption experiments. The field experiment was performed in a co-contaminated clay purple soil (stagnic anthrosols). Along with a control experiment (T1), CA-1 and CA-2 were tested at different rates including 750, 1500, 3000 and 6000 kg ha -1 by growing wheat as the test crop. The obtained results revealed that the highest dose of both composites (T5: 6000 kg ha -1 and T9: 6000 kg ha -1 ) increased the soil pH to 6.85 and 6.81, respectively as compared to the control (5.63). DTPA-extractable Cd and Pb contents decreased with composite treatments (T7 and T4) at harvest stage samples. Metal fractionation depicted that application of amendments decreased the exchangeable fraction at harvesting stage. Application of CA-2 and CA-1 (3000 kg ha -1 ) significantly increased the plant biomass (by 28% and 24%, respectively) and grain yield (by 26% and 22%, respectively) of wheat. Furthermore, batch sorption experiment results revealed that Langmuir adsorption model better fitted the sorption results with R 2 values ranging between 0.99 and 0.91 for Cd and Pb, respectively. CA-1 and CA-2 exhibited the maximum adsorption capacity for Cd with no significant difference among treatments but Pb adsorption capacity was highest in CA-1 followed by CA-2 and control. The results of our experiments revealed that the application of organics combined with inorganic materials enhanced Cd and Pb immobilization and sorption, consequently reducing metals availability in laboratory and field conditions. Moreover, for field trials, application of the composite amendments at 3000 kg ha -1 emerged as the suitable treatment for tested wheat-grown area., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Present study reports the laboratory and field scale application of different organic and inorganic amendments to immobilize cadmium (Cd) and lead (Pb) in a co-contaminated alluvial paddy soil. For that purpose, lime, biochar, Fe-biochar and two composite amendments (CA) composed of biochar, lime, sepiolite and zeolite (CA1: composite amendment 1) and manure, lime and sepiolite (CA2: composite amendment 2) were firstly tested in an incubation experiment to ameliorate Cd and Pb co-contaminated alluvial soil. It was observed that liming and CA2 elevated the soil pH and reduced DTPA extractable Cd and Pb in the incubated soil leading to higher metal immobilization. Therefore, efficiency of lime and CA2 was further investigated in field conditions with mid rice as the test crop to evaluate field scale immobilization and precise application rate for the tested soil type. DTPA and CaCl 2 extractable Cd (46 and 51%) and Pb (68 and 70%) in field soil were decreased with applied treatments. Speciation of Cd and Pb also promoted conversion of metal exchangeable contents to less-available forms. Activated functional groups on amendments' surface ( &#95; OH bonding, C &#95; O and CO, -O-H, Si-O-Si, carboxylic and ester groups) sequestered metals by precipitation, adsorption, ion exchange or electro static attributes. Application of lime at 2400 kg/acre (T4) and CA2 at 1200 kg/acre was more effective in reducing rice shoot and grains metal contents. Moreover, obtained results in terms of pH, extractable content, speciation and yield, and microanalysis of amendments highlights the remarkable efficiency of lime and composite amendment to sorb Cd and Pb providing the key evidence of these amendments for metals immobilization and environmental remediation. Considering these results, lime and CA2 are potential amendments for co-contaminated rice field especially in context of alluvial soil., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Forty oilseed sunflower cultivars were screened in two soil types for phytoremediation of Cd coupled with maximum biomass yield and oil production. Several cultivars exhibited a significant difference in biomass and yield with enhanced uptake in shoots and low accumulation in roots from two Cd-contaminated soil types, an Oxisol and an Iceptisol. The Transfer Factor of Cd was >1 in several cultivars in both soil types, where as a significant difference in phytoextraction of Cd was observed in the Oxisol (acidic soil), greater than in the Inceptisol (alkaline soil). The results revealed that of the 40 cultivars, S9178, Huanong 667in the Oxisol and cvs. DW 667, HN 667, Huanong 667 and 668F1 in the Inceptisol showed a high biomass, better yield and enhanced accumulation of Cd in the shoots but a lesser accumulation in oil. The screened cultivar S 9178 produced the greatest amount of oil (55.6%) with 77% oleic acid, which makes it suitable for human consumption. Cultivar Huanong 667 was found to be the highest accumulating cultivar in both soil types. It is therefore suggested that some sunflower cultivars do exhibit phytoremediation potential together with agro-production potential., (Copyright © 2019 Elsevier Inc. All rights reserved.)

The willow ( Salix spp.) is a potential accumulator of cadmium (Cd)/zinc (Zn), and the interaction between Cd and Zn is an important factor influencing their phytoextraction potentials. In this study, interactions between Cd and Zn in uptake, accumulation and bioavailability for Salix integra clone SI63 were investigated through nutrient solution and soil culture methods. The result of the soil culture showed that Cd had additive effects for Zn-caused biomass reduction. The result from nutrient solution indicated that added Zn showed antagonistic (low Cd level) or synergistic (moderate and high Cd levels) effects on shoot Cd accumulation and antagonistic effects on root Cd accumulation. Irrespective of nutrient solution or soil culture experiment, Cd addition always had antagonistic effects on Zn accumulation in both shoots and roots. Under Cd10Zn50 condition, the clone accumulated higher Cd and Zn concentrations (95 μg g -1 Cd and 165 μg g -1 Zn) in shoots. Cd slightly increased bioavailable Zn in the rhizosphere, and EDTA well predicted bioavailable Cd and Zn in the rhizosphere. Interactions of Cd-Zn markedly changed their respective phytoextraction potentials, especially for Zn. This result will provide a new insight into improving phytoextraction potentials of both Cd and Zn using willows through applying metal interactions.

Tetracycline (TC) can enter the human body via the soil-vegetable-human food chain; therefore, it is necessary to understand the toxicity of TC to humans through vegetables grown on contaminated soils. The present study combined an enzyme-linked immunosorbent assay method and an HL-7702 cell model and assessed the bioavailability and toxicity of TC from pak choi (Brassica campestris L. ssp. chinensis) grown on TC-contaminated soils. The results showed that the degradation rate of TC in black soil was significantly higher than that in purplish clay, while the results for TC uptake in pak choi were opposite. The bioaccessibility of TC was found to be higher in pak choi grown on purplish clay (5.67-7.59%) than in that grown on black soil (5.22-6.77%). It is suggested that soil properties contribute to the uptake of TC by pak choi. More fertile soil contained lower TC concentrations and thus mediated lower TC toxicity to humans. It may seem comforting that TC concentrations in the edible parts of pak choi are often found to be below safe limits. However, the TC diagnosis method showed that even moderate increases in TC concentrations in pak choi may induce oxidative stress, liver injury, mitochondrial cristae and rough endoplasmic reticulum swelling, and early apoptosis in liver cells HL-7702. The pak choi grown in purplish clay showed higher TC cytotoxicity than that grown in black soil. The TC cytotoxicity of raw pak choi was found to be higher than that of cooked pak choi. These results provide direct evidence of effective ways to prevent TC toxicity in humans., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Impact of different biochars supplemented (10% w/w) to promote vermicomposting of sewage sludge (SS) and kitchen waste (KW) mixture (SS + KW, 70:30) was studied on the growth, reproduction and survival of earthworms, and ultimately the quality of vermicompost. Four types of biochar used as secondary material for preincubation (16 days) and vermicomposting (30 days) were: pine tree biochar (PTB), poplar plant biochar (PPB), wetland plant biochar (WPB) and yard waste biochar (YWB). Preincubation and vermicomposting of biomass mixture were undertaken in 60 L and 2 L capacity round-shaped bioreactors, respectively. Samples of biomass undergoing degradation were drawn after every 2 days during preincubation and with 5 days interval during vermicomposting to analyze them for plant nutrients and heavy metals contents. Amendment of vermicompost substrate (SS + KW) with biochars; PTB, PPB, WPB and YWB increased the reproduction rate of earthworms (Eisenia fetida) by 44.6, 53.9, 29.3 and 38.8%, respectively as compared to control (no biochar, NB). There has been significant reduction in total content of Cd (0.2-5.1%), Cr (7.3-10.8%), Cu (3.1-7.4%), Mn (3.2-8.4%), Pb (9.0-45.9%) and Zn (1.1-5.7%) by the application of different biochars as compared to NB after vermicomposting. The SEM/EDS images also reflected reduced concentration of these heavy metals in the final vermicompost as compared to initial mixtures. Progressively, biochar amendments increased the concentration of all macronutrients, viz., TN (15.8-31.0%), TP (8.6-9.9%), TK (2.8-17.3%), Ca (4.1-9.9%) and Mg (0.8-12.2%); while, reduced the pH (1.9-2.3%), content of Na (6.6-22.3%), TOC (6.6-15.4%), OM (5.0-8.2%) and C:N ratio (2.6-18.9%). Earthworm body accumulation factor (BAF) of heavy metals was: Cd > Zn > Pb > Cu > Mn > Cr at the termination stage of experiment. In conclusion, amending the SS + KW mixture with 10% (w/w) PPB for vermicomposting rendered higher count of cocoons, growth rate and reproduction rate of earthworms, which ultimately produce nutrients-rich vermicompost lower in heavy metals., (Copyright © 2019 Elsevier B.V. All rights reserved.)

The identification of high yield genotypes that are capable of accumulating multiple heavy metals in the non-edible parts (roots and shoots), but not in the edible parts (seeds) and have desired nutritional value is necessary for accomplishing phytoremediation coupled with agro-production. In this study, 17 fava bean genotypes were screened in two different field conditions to examine their phytoremediation potential in terms of uptake and translocation of Cd and Pb. Ten genotypes, LBAO, JNJX, DCAN, QXCJ, QIKM, LXYC, YDL6, RBCD, QPID and ZHW6 were found as the best accumulators for Cd and Pb with permissible limit of metals in seeds. The concentration of plant nutrients were genotype and soil type dependent and there was a significant correlation between these two factors. Furthermore, the three genotypes DCAN, LBAO and LXYC showed best performance in alluvial soil type while QPID, RBCD and LXYC were the best in red soil type. Genotype LXYC was similar for both soil types and appeared to be the best fit for phytoremediation coupled with agro-production for slightly or moderately Cd and Pb co-contaminated soil. Therefore, fava bean LXYC genotype is suggested as a potential candidate for phytoremediation of Cd/Pb co-contaminated soils coupled with agro-production., (Copyright © 2018 Elsevier Inc. All rights reserved.)

Cadmium contamination in soil, water and food has become a global problem since last century's industrial and agricultural revolution. It is a highly toxic metal with serious consequences on human and animal health. Different natural and anthropogenic sources are responsible for Cd release in the soil which ultimately leads to the food chain. Cd persists in soil for long durations due to its minimal microbial or chemical loss. There are various physical, chemical or biological techniques which are helpful to minimize Cd risk in food chain. Among them, in-situ immobilization with organic, inorganic or clay amendments is a cost-effective and an environment friendly strategy to remediate Cd polluted sites. Lime, biochar, organic wastes, phosphorus fertilizers, sepiolite, zeolite, hydroxyapatite and bentonite are commonly used amendments for amelioration of Cd contaminated soils. These amendments reduce Cd uptake and enhance immobilization by adsorption, complexation, and precipitation processes. This review is aimed to provide a comprehensive note on Cd toxicity in humans and environment, its immobilization by different agents through variety of processes, and comparison of technologies for Cd removal from contaminated sites., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Field experiments were conducted in two consecutive rice-wheat cropping seasons on a yellow clay soil to assess the efficacy of organic and inorganic amendments for cadmium (Cd) and lead (Pb) immobilization. Amendments were applied alone and in combinations to compare their efficacy for metals immobilization. Composite amendment of GSA-4 (Green Stabilizing Agent) and biochar resulted in higher biomass and grains yield for both rice (Oryza sativa L.) and wheat (Triticum aestivum L.). Liming, DEK1 (Di Kang No. 1) or GSA-4 amendment increased soil pH from 6.34 to 7.35, 7.20 and 7.15, respectively. Soil amendments significantly reduced DTPA extractable Cd and Pb in soil at wheat harvest. Cadmium and Pb fractionation showed a significant decrease in the extractable fractions by the amendment of biochar (34% and 25%) or GSA-4 (35% and 26%, respectively). GSA-4 and biochar amendment enhanced metals immobilization and reduced their uptake by plant and subsequent accumulation in the grains of rice and wheat, particularly with GSA-4. These results indicate that GSA-4 and biochar, especially their combination, have great potential for application to remediate Cd and Pb contaminated soils., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Heavy metal contamination of agricultural soils has posed a risk to environment and human health. The present study was conducted to assess the effectiveness of soil amendments for reducing cadmium (Cd) and lead (Pb) uptake by rice (Oryza sativa L) in a contaminated field. The soil amendments used include lime, DaSan Yuan (DASY), DiKang No.1 (DEK1), biochar, Fe-biochar, Yirang, phosphorus fertilizer, (Green Stabilizing Agent) GSA-1, GSA-2, GSA-3, and GSA-4, applied at 1% rate in a field experiment. The results exposed that GSA-4 treatment showed best effects on reducing Cd and Pb phytoavailability in soil and uptake by early rice. Linear increase in pH (i.e. 5.69 to 6.75) was recorded in GSA-4 amended soil from sowing to the 3rd month of growth season. GSA-4 decreased DTPA extractable contents of cadmium (Cd) from 0.324 to 0.136 mg kg -1 soil and lead (Pb) from 53.21 to 24.68 mg kg -1 soil at 90 days of amendment. Treatment with GSA-4 improved rice growth (56%) and grains yield (42%). The enhancement effects on grain yield may be result from the positive effects of GSA-4 application on increasing photosynthesis (116%) and transpiration rate (152%) as compared to the control. Significant reduction in Cd and Pb uptake in shoot (42% and 44%) and in grains (77 and 88%), was observed, respectively in GSA-4 treatment as compared with the control. Moreover, negative correlation was recorded between DTPA extractable Cd/Pb and soil pH that directly depended on applied amendments. In short, use of combined amendment (GSA-4) was more effective for immobilizing heavy metals in contaminated paddy field, and secures rice safe production, as compared other tested amendment products.