The effect of long-term farming on the cation exchange capacity (CEC), organic carbon content, soil solution composition, and aggregate stability was investigated using contrasting soils from 4 sites in the Mid North of South Australia. Undisturbed and farmed profiles were characterised at each site. Farming led to a 10–50% decrease, approximately, in organic matter and CEC in the surface horizon. Scanning electron microscopic study of the surface and selected subsurface soils revealed poor aggregation, compaction, reduced porosity, and a decrease in aggregate particle size in the farmed surface soils. Intra-aggregate binding in the undisturbed soils appeared to be largely due to fungal hypha, with the roots largely contributing to inter-aggregate binding of soil particles. Electrical conductivity (EC) of soil solutions was generally 2–3 times higher in the undisturbed soils than farmed soils, suggesting increased leaching of ions associated with loss of tree cover. This was also supported by a decrease in the concentrations of mobile ions such as Cl-and Na+ in the farmed soils. The concentrations of Na+ and K+ decreased with farming leading to a decrease in the Gapon selectivity constant for Na–Ca and K–Ca exchange. The changes in soil solution composition together with the decline in organic matter concentrations resulted in increased sensitivity of soils to dispersion.