1. Chemosensitizing Effect of Cernumidine Extracted from Solanum cernuum on Bladder Cancer Cells in Vitro.
- Author
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Miranda MA, Mondal A, Sachdeva M, Cabral H, Neto YAAH, Khan I, Groppo M, McChesney JD, and Bastos JK
- Subjects
- Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic isolation & purification, Apoptosis drug effects, Caffeic Acids chemistry, Caffeic Acids isolation & purification, Cell Movement drug effects, Cell Proliferation drug effects, Drug Screening Assays, Antitumor, Guanidines chemistry, Guanidines isolation & purification, Humans, Membrane Potential, Mitochondrial drug effects, Microscopy, Fluorescence, Mitochondria drug effects, Mitochondria metabolism, Plant Leaves chemistry, Tumor Cells, Cultured, Urinary Bladder Neoplasms metabolism, Urinary Bladder Neoplasms pathology, Antineoplastic Agents, Phytogenic pharmacology, Caffeic Acids pharmacology, Guanidines pharmacology, Solanum chemistry, Urinary Bladder Neoplasms drug therapy
- Abstract
Cernumidine (CER) is a guanidinic alkaloid isolated from Solanum cernuum leaves. In this work, we investigated the cytotoxicity, chemosensitizing effect of cernumidine to cisplatin (cDDP) and the possible mechanism of action of the combination on bladder cancer cells. Cernumidine showed cytotoxicity and could sensitize bladder cancer cells to cisplatin. The combination of CER+cDDP inhibited cell migration on T24 cells. CER+cDDP down-regulated MMP-2/9 and p-ERK1/2, while it increased EGFR activity corroborating the observed cell migration inhibition. Down-regulation of Bcl-2 and up-regulation pro-apoptotic Bax and further depletion of the mitochondrial membrane potential (ΔΨm) indicates that mitochondria play a central role in the combination treatment inducing the mitochondrial signaling pathway of apoptosis in T24 cells. Our data showed that the alkaloid cernumidine is worthy of further studies as a chemosensitizing agent to be used in complementary chemotherapy., (© 2019 Wiley-VHCA AG, Zurich, Switzerland.)
- Published
- 2019
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