9 results on '"Zhao, Wancheng"'
Search Results
2. CCMRD: a solid-state NMR database for complex carbohydrates
- Author
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Kang, Xue, Zhao, Wancheng, Dickwella Widanage, Malitha C., Kirui, Alex, Ozdenvar, Uluc, and Wang, Tuo
- Published
- 2020
- Full Text
- View/download PDF
3. Charting the solid‐state NMR signals of polysaccharides: A database‐driven roadmap.
- Author
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Zhao, Wancheng, Debnath, Debkumar, Gautam, Isha, Fernando, Liyanage D., and Wang, Tuo
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CHITIN , *POLYSACCHARIDES , *PLANT polymers , *NUCLEAR magnetic resonance , *GLUCANS , *GLYCANS - Abstract
Solid‐state nuclear magnetic resonance (ssNMR) measurements of intact cell walls and cellular samples often generate spectra that are difficult to interpret due to the presence of many coexisting glycans and the structural polymorphism observed in native conditions. To overcome this analytical challenge, we present a statistical approach for analyzing carbohydrate signals using high‐resolution ssNMR data indexed in a carbohydrate database. We generate simulated spectra to demonstrate the chemical shift dispersion and compare this with experimental data to facilitate the identification of important fungal and plant polysaccharides, such as chitin and glucans in fungi and cellulose, hemicellulose, and pectic polymers in plants. We also demonstrate that chemically distinct carbohydrates from different organisms may produce almost identical signals, highlighting the need for high‐resolution spectra and validation of resonance assignments. Our study provides a means to differentiate the characteristic signals of major carbohydrates and allows us to summarize currently undetected polysaccharides in plants and fungi, which may inspire future investigations. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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4. Polysaccharide assemblies in fungal and plant cell walls explored by solid-state NMR.
- Author
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Fernando, Liyanage D., Zhao, Wancheng, Gautam, Isha, Ankur, Ankur, and Wang, Tuo
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PLANT cell walls , *FUNGAL cell walls , *POLYSACCHARIDES , *PLANT cells & tissues , *PECTINS , *PLANT stems , *CELL anatomy - Abstract
Structural analysis of macromolecular complexes within their natural cellular environment presents a significant challenge. Recent applications of solid-state NMR (ssNMR) techniques on living fungal cells and intact plant tissues have greatly enhanced our understanding of the structure of extracellular matrices. Here, we selectively highlight the most recent progress in this field. Specifically, we discuss how ssNMR can provide detailed insights into the chemical composition and conformational structure of pectin, and the consequential impact on polysaccharide interactions and cell wall organization. We elaborate on the use of ssNMR data to uncover the arrangement of the lignin-polysaccharide interface and the macrofibrillar structure in native plant stems or during degradation processes. We also comprehend the dynamic structure of fungal cell walls under various morphotypes and stress conditions. Finally, we assess how the combination of NMR with other techniques can enhance our capacity to address unresolved structural questions concerning these complex macromolecular assemblies. [Display omitted] The advance in solid-state NMR spectroscopy enables the analysis of polysaccharide and protein assemblies in living fungal cells and intact plant tissues at atomic-to-nanometer resolution. In this review, Fernando et al. discuss the most recent conceptual breakthroughs in cell wall structural biology. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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5. Current limitations of solid-state NMR in carbohydrate and cell wall research.
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Zhao, Wancheng, Deligey, Fabien, Chandra Shekar, S., Mentink-Vigier, Frederic, and Wang, Tuo
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POLARIZATION (Nuclear physics) , *MAGIC angle spinning , *CARBOHYDRATES , *NUCLEAR spin , *BIRADICALS , *SPECTRAL line broadening - Abstract
[Display omitted] • Limitations and open questions of solid-state NMR for carbohydrate and cell wall studies. • Difficulty in resonance assignment is caused by structural complexity of biopolymers. • Methods are needed to better use the growing dataset for restraining the packing interface. • Improved sample preparation and technology of DNP will benefit biomaterial research. High-resolution investigation of cell wall materials has emerged as an important application of biomolecular solid-state NMR (ssNMR). Multidimensional correlation experiments have become a standard method for obtaining sufficient spectral resolution to determine the polymorphic structure of carbohydrates and address biochemical questions regarding the supramolecular organization of cell walls. Using plant cellulose and matrix polysaccharides as examples, we will review how the multifaceted complexity of polysaccharide structure is impeding the resonance assignment process and assess the available biochemical and spectroscopic approaches that could circumvent this barrier. We will emphasize the ineffectiveness of the current methods in reconciling the ever-growing dataset and deriving structural information. We will evaluate the protocols for achieving efficient and homogeneous hyperpolarization across the cell wall material using magic-angle spinning dynamic nuclear polarization (MAS-DNP). Critical questions regarding the line-broadening effects of cell wall molecules at cryogenic temperature and by paramagnetic biradicals will be considered. Finally, the MAS-DNP method will be placed into a broader context with other structural characterization techniques, such as cryo-electron microscopy, to advance ssNMR research in carbohydrate and cell wall biomaterials. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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- View/download PDF
6. A 13C three-dimensional DQ-SQ-SQ correlation experiment for high-resolution analysis of complex carbohydrates using solid-state NMR.
- Author
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Shekar, S. Chandra, Zhao, Wancheng, Fernando, Liyanage D., Hung, Ivan, and Wang, Tuo
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FUNGAL cell walls , *PLANT cell walls , *CARBOHYDRATES , *CELLULOSE synthase , *ASPERGILLUS fumigatus , *PHYTOPATHOGENIC microorganisms - Abstract
[Display omitted] • An 3D CCC (DQ-SQ-SQ) experiment facilitates the analysis of carbohydrates. • Unprecedented resolution enables resonance assignments of cellular carbohydrate. • Long-range structural restraints are obtained to understand microfibril structure. • Results provide novel insights to the cell walls of plants and fungal pathogens. Complex carbohydrates are the key components of the protective cell walls of microbial pathogens and the bioenergy reservoir in plants and algae. Structural characterization of these polymorphic molecules requires assistance from multidimensional 13C correlation approaches. To facilitate the analysis of carbohydrate structure using solid-state NMR, we present a three-dimensional (3D) 13C-13C-13C experiment that includes a double-quantum (DQ) dimension and is thus free of the cube's body diagonal. The enhanced resolution supports the unambiguous resonance assignment of many polysaccharides in plant and fungal cell walls using uniformly 13C-labeled cells of spruce and Aspergillus fumigatus. Long-range structural restraints were effectively obtained to revisit our understanding of the spatial organization of plant cellulose microfibrils. The method is widely applicable to the investigations of cellular carbohydrates and carbon-based biomaterials. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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7. Effect of cross polarization radiofrequency phases on signal phase.
- Author
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Shekar, S. Chandra, Zhao, Wancheng, Weldeghiorghis, Thomas K., and Wang, Tuo
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RADIO frequency , *MOLECULAR rotation - Abstract
Utilizing phases of radio frequency (RF) pulses to manipulate spin dynamics is routine in NMR and MRI, leading to spectacular techniques like phase cycling. In a very different area, cross polarization (CP) also has a long history as part of a vast number of solid-state NMR pulse sequences. However, a detailed study devoted to the effect of CP RF phases on NMR signal, seems not to be readily available. From first principles, we arrive at a simple dependence of NMR signal on arbitrary CP RF phases, for static and MAS conditions, accompanied by experimental verification. In the process, the CP propagator emerges as a product of RF "pulses" and a period of "free precession", conforming to coherence transfer pathway theory. The theoretical expressions may lend confidence for dealing with CP blocks with tunable phases in pulse sequences. [Display omitted] • Effect of arbitrary RF phases during CP NMR explored from first principles. • S-spin NMR signal acquires same phase as arbitrary RF phases during CP. • This holds for both static and MAS, as evolution operator structure is preserved. • Extensively experimentally validated for 1H-X(=13C, 15N, 31P) CP (static and MAS). [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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8. Solid-state NMR of plant and fungal cell walls: A critical review.
- Author
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Zhao, Wancheng, Fernando, Liyanage D., Kirui, Alex, Deligey, Fabien, and Wang, Tuo
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FUNGAL cell walls , *PLANT cell walls , *ANTIFUNGAL agents , *MAGIC angle spinning , *PHYTOPATHOGENIC fungi , *CELL anatomy , *BACTERIAL cell walls , *PEPTIDOGLYCANS - Abstract
The cell walls of plants and microbes are a central source for bio-renewable energy and the major targets of antibiotics and antifungal agents. It is highly challenging to determine the molecular structure of complex carbohydrates, protein and lignin, and their supramolecular assembly in intact cell walls. This article selectively highlights the recent breakthroughs that employ 13C/15N solid-state NMR techniques to elucidate the architecture of fungal cell walls in Aspergillus fumigatus and the primary and secondary cell walls in a large variety of plant species such as Arabidopsis , Brachypodium , maize, and spruce. Built upon these pioneering studies, we further summarize the underexplored aspects of fungal and plant cell walls. The new research opportunities introduced by innovative methods, such as the detection of proton and quadrupolar nuclei on ultrahigh-field magnets and under fast magic-angle spinning, paramagnetic probes, natural-abundance DNP, and software development, are also critically discussed. Image 1 • Review of recent advances in carbohydrate and cell wall research. • Packing of polysaccharides, proteins, and lignin in plants and pathogenic fungi. • Unresolved questions of carbohydrate structures and cell wall assembly. • Outlook: proton detection, quadrupolar nuclei, PRE, DNP, ultrahigh field, and database. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
9. A pectin methyltransferase modulates polysaccharide dynamics and interactions in Arabidopsis primary cell walls: Evidence from solid-state NMR.
- Author
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Kirui, Alex, Du, Juan, Zhao, Wancheng, Barnes, William, Kang, Xue, Anderson, Charles T., Xiao, Chaowen, and Wang, Tuo
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PECTINS , *CELLULOSE synthase , *PLANT cell walls , *ARABIDOPSIS , *POLYMER structure , *CELLULAR mechanics - Abstract
Plant cell walls contain cellulose embedded in matrix polysaccharides. Understanding carbohydrate structures and interactions is critical to the production of biofuel and biomaterials using these natural resources. Here we present a solid-state NMR study of cellulose and pectin in 13C-labeled cell walls of Arabidopsis wild-type and mutant plants. Using 1D 13C and 2D 13C–13C correlation experiments, we detected a highly branched arabinan structure in qua2 and tsd2 samples, two allelic mutants for a pectin methyltransferase. Both mutants show close physical association between cellulose and the backbones of pectic homogalacturonan and rhamnogalacturonan-I. Relaxation and dipolar order parameters revealed enhanced microsecond dynamics due to polymer disorder in the mutants, but restricted motional amplitudes due to tighter pectin-cellulose associations. These molecular data shed light on polymer structure and packing in these two pectin mutants, helping to elucidate how pectin could influence cell wall architecture at the nanoscale, cell wall mechanics, and plant growth. • Pectin structure and cellulose packing were assessed using solid-state NMR. • Two pectin mutants, tsd2 and qua2 , were compared to wild-type Arabidopsis. • In these mutants, arabinan content is increased, with a higher degree of branching. • Mutants show tighter packing between cellulose and pectic backbones (HG and RG-I). • The motion of HG and RG-I is faster in the mutants, but with smaller amplitudes. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
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