18 results on '"Bernal, Rafael"'
Search Results
2. Occurrence and genetic diversity of Enterocytozoon bieneusi (Microsporidia) in owned and sheltered dogs and cats in Northern Spain
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Dashti, Alejandro, Santín, Mónica, Cano, Lourdes, de Lucio, Aida, Bailo, Begoña, de Mingo, Marta Hernández, Köster, Pamela C., Fernández-Basterra, José A., Aramburu-Aguirre, Juan, López-Molina, Nuria, Fernández-Crespo, Juan C., Calero-Bernal, Rafael, and Carmena, David
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- 2019
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3. Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks
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Fernández-Escobar, Mercedes, Calero-Bernal, Rafael, Benavides, Julio, Regidor-Cerrillo, Javier, Guerrero-Molina, María Cristina, Gutiérrez-Expósito, Daniel, Collantes-Fernández, Esther, and Ortega-Mora, Luis Miguel
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- 2020
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4. Protist enteroparasites in wild boar (Sus scrofa ferus) and black Iberian pig (Sus scrofa domesticus) in southern Spain: a protective effect on hepatitis E acquisition?
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Rivero-Juarez, Antonio, Dashti, Alejandro, López-López, Pedro, Muadica, Aly Salimo, Risalde, Maria de los Angeles, Köster, Pamela C., Machuca, Isabel, Bailo, Begoña, de Mingo, Marta Hernández, Dacal, Elena, García-Bocanegra, Ignacio, Saugar, José M., Calero-Bernal, Rafael, González-Barrio, David, Rivero, Antonio, Briz, Verónica, and Carmena, David
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- 2020
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5. Zoonotic Enterocytozoon bieneusi genotypes in free-ranging and farmed wild ungulates in Spain
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Dashti, Alejandro, Santín, Mónica, Köster, Pamela C, Bailo, Begoña, Ortega, Sheila, Imaña, Elena, Habela, Miguel Ángel, Rivero-Juarez, Antonio, Vicente, Joaquin, Conejero, Carles, González-Crespo, Carlos, Garrido, Cristina, Gassó, Diana, Andrea Murillo, Diana, Serrano, Emmanuel, Mentaberre, Gregorio, Torres-Blas, Irene, Estruch, Josep, Pastor, Josep, Ramón López-Olvera, Jorge, Escobar-González, María, Valldeperes, Marta, Mesalles, Montse, López, Omar, Álvarez, Raquel, Cuenca, Rafaela, Velarde, Roser, Lavín, Santiago, Arnal, Maria C, Fernánez de Luco, Daniel, Morrondo, Patrocinio, Armenteros, José A, Balseiro, Ana, Cardona, Guillermo A, Martínez-Carrasco, Carlos, Ortiz, José Antonio, Calero-Bernal, Rafael, Carmena, David, González-Barrio, David, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Ayuntamiento de Barcelona, and Ministerio de Ciencia e Innovación (España)
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Swine Diseases ,molecular diversity ,China ,Sheep ,Enterocytozoon bieneusi ,Genotype ,Swine ,Deer ,Sus scrofa ,Sheep Diseases ,Animals, Wild ,General Medicine ,Enterocytozoon ,Feces ,Infectious Diseases ,Spain ,Microsporidiosis ,wild ungulates ,Prevalence ,Animals ,Humans ,Phylogeny - Abstract
WE&H group: Carles Conejero, Carlos González-Crespo, Cristina Garrido, Diana Gassó, Diana Andrea Murillo, Emmanuel Serrano, Gregorio Mentaberre, Irene Torres-Blas, Josep Estruch, Josep Pastor, Jorge Ramón López-Olvera, María Escobar-González, Marta Valldeperes, Montse Mesalles, Omar López, Raquel Álvarez, Rafaela Cuenca, Roser Velarde, Santiago Lavín., Microsporidia comprises a diverse group of obligate, intracellular, and spore-forming parasites that infect a wide range of animals. Among them, Enterocytozoon bieneusi is the most frequently reported species in humans and other mammals and birds. Data on the epidemiology of E. bieneusi in wildlife are limited. Hence, E. bieneusi was investigated in eight wild ungulate species present in Spain (genera Ammotragus, Capra, Capreolus, Cervus, Dama, Ovis, Rupicapra, and Sus) by molecular methods. Faecal samples were collected from free-ranging (n = 1058) and farmed (n = 324) wild ungulates from five Spanish bioregions. The parasite was detected only in red deer (10.4%, 68/653) and wild boar (0.8%, 3/359). Enterocytozoon bieneusi infections were more common in farmed (19.4%, 63/324) than in wild (1.5%, 5/329) red deer. A total of 11 genotypes were identified in red deer, eight known (BEB6, BEB17, EbCar2, HLJD-V, MWC_d1, S5, Type IV, and Wildboar3) and three novel (DeerSpEb1, DeerSpEb2, and DeerSpEb3) genotypes. Mixed genotype infections were detected in 15.9% of farmed red deer. Two genotypes were identified in wild boar, a known (Wildboar3) and a novel (WildboarSpEb1) genotypes. All genotypes identified belonged to E. bieneusi zoonotic Groups 1 and 2. This study provides the most comprehensive epidemiological study of E. bieneusi in Spanish ungulates to date, representing the first evidence of the parasite in wild red deer populations worldwide. Spanish wild boars and red deer are reservoir of zoonotic genotypes of E. bieneusi and might play an underestimated role in the transmission of this microsporidian species to humans and other animals., This work was supported by Health Institute Carlos III (ISCIII), Spanish Ministry of Economy and Competitiveness, under project PI19CIII/00 029. Sample collection in Catalonia was supported by the Council of Barcelona through contracts 13/051, 15/0174, 16/0243 and 16/0243-00-PR/01, from the Spanish Ministry of Economy and Competitiveness under research grants CGL2012- 40043-C02-01, CGL2012-40043-C02-02, and from the Spanish Ministry of Science and Innovation under research grant CGL2016-80543-P PID2020-115046GB-I00, and USDA-ARS Project No: 8042 -32000-112 -00-D., Antonio Rivero-Juárez is the recipient of a Miguel Servet Research Contract by the Ministerio de Ciencia, Promoción y Universidades of Spain (CP18/00111). D.G.B. is the recipient of a Sara Borrell Research Contract (CD19CIII/00011) funded by the Spanish Ministry of Science, Innovation and Universities. A.D. is the recipient of a PFIS contract (FI20CIII/00002) funded by the Spanish Ministry of Science and Innovation and Universities., Medical Mycology
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- 2022
6. Diarrhoea‐causing enteric protist species in intensively and extensively raised pigs (Sus scrofa domesticus) in Southern Spain. Part II: Association with Hepatitis E virus susceptibility.
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Rivero‐Juárez, Antonio, Dashti, Alejandro, Santín, Mónica, Köster, Pamela C., López‐López, Pedro, Risalde, María A., García‐Bocanegra, Ignacio, Gómez‐Villamandos, José Carlos, Caballero‐Gómez, Javier, Frías, Mario, Bailo, Begoña, Ortega, Sheila, Muadica, Aly Salimo, Calero‐Bernal, Rafael, González‐Barrio, David, Rivero, Antonio, Briz, Verónica, and Carmena, David
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CRYPTOSPORIDIUM ,HEPATITIS E virus ,SWINE ,SPECIES ,ANIMAL populations ,FOOD pathogens - Abstract
Enteropathogenic parasites can infect a wide range of mammals, including humans, supposing an important zoonotic risk. Hepatitis E virus (HEV) is an emerging foodborne pathogen of increasing public health relevance, affecting both humans and animal populations. Because both microorganisms share faecal‐oral transmission route they may constitute an excellent model to evaluate the interplay between them. Thus, we aim to evaluate the viral‐parasite interactions at the enteric interface in swine. We included pigs of two different breeds farming in South Spain under different production systems. We compared the HEV prevalence by the presence of Giardia duodenalis, Cryptosporidium spp., Balantioides coli, Blastocystis sp. and Enterocytozoon bieneusi in faecal samples. The HEV prevalence was 13.1 (62 out 475, 95% CI: 10.2–16.4). Those pigs infected with Cryptosporidium spp. showed a higher prevalence of HEV (30.8 vs. 12%; p =.012). In the same way, animals bearing E. bieneusi seem to have a higher rate of HEV infection (24.2 vs. 12.2%; p =.06). According to their location in the gut, animals bearing intracellular enteroparasites showed a higher HEV prevalence than those uninfected (29.6 vs. 12.7%; p =.038), meanwhile those carrying extracellular enteroparasites had a lower likelihood to be infected by HEV than those uninfected (12.1 vs. 23.1%; p =.071). Those animals bearing both types of enteroparasites showed a similar prevalence of HEV infection than those exhibiting negative for both (20.8 vs. 26.1%; p =.763). Our study provides evidence that intracellular and extracellular enteroparasites modulate the susceptibility to HEV infection in pigs. Meanwhile, the presence of extracellular enteroparasites shows a protective effect on the risk of HEV acquisition in swine, whereas intracellular enteroparasites seems to have the opposite effect, favouring the HEV infection. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Diarrhoea‐causing enteric protist species in intensively and extensively raised pigs (Sus scrofa domesticus) in Southern Spain. Part I: Prevalence and genetic diversity.
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Dashti, Alejandro, Rivero‐Juárez, Antonio, Santín, Mónica, George, Nadja S., Köster, Pamela C., López‐López, Pedro, Risalde, María A, García‐Bocanegra, Ignacio, Gómez‐Villamandos, Jose Carlos, Caballero‐Gómez, Javier, Frías, Mario, Bailo, Begoña, Ortega, Sheila, Muadica, Aly Salimo, Calero‐Bernal, Rafael, González‐Barrio, David, Rivero, Antonio, Briz, Verónica, and Carmena, David
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CRYPTOSPORIDIUM ,SWINE ,GENETIC variation ,SPECIES - Abstract
Numerous protist species are shared between humans and pigs. Among those, Giardia duodenalis, Cryptosporidium spp. and Balantioides coli have a clear public and animal health significance. For others such as Enterocytozoon bieneusi and Blastocystis sp., their impact on animal health has not been fully established. Little information is currently available on the molecular diversity of these protists in swine populations. To fill this gap, we molecularly assessed G. duodenalis, Cryptosporidium spp., B. coli, Blastocystis sp. and E. bieneusi in faecal samples from Iberian and Large White pigs raised under different (intensive and/or extensive) management systems in southern Spain. A total of 151 extensively raised Iberian pigs, 140 intensively raised Iberian pigs, and 184 intensively raised Large White pigs were investigated. Blastocystis sp. was the agent most prevalently found (47.8%), followed by B. coli (45.5%), G. duodenalis (10.7%), E. bieneusi (6.9%), and Cryptosporidium spp. (5.5%). Blastocystis sp. was significantly less prevalent in intensively raised Iberian pigs (22.9%) than in their extensively raised counterparts (51.0%) or in intensively raised Large White pigs (64.1%). A significantly higher prevalence was found for G. duodenalis, Cryptosporidium spp., and E. bieneusi in Large White pigs than Iberian pigs. Balantioides coli was similarly distributed (40.0–51.1%) in all three investigated swine populations. Sequence analyses revealed the presence of G. duodenalis assemblage E, two Cryptosporidium species (Cryptosporidium scrofarum and Cryptosporidium suis), B. coli (genotypes A and B), Blastocystis sp. (ST1, ST3, and ST5), and E. bieneusi (EbpA, EbpC, EbpD, O, and a novel genotype named PigSpEb2). Novel genotype PigSpEb2 was found alone or in combination with EbpA. Data suggest a widespread exposure to protist enteroparasites in domestic pig populations irrespectively of breed and raising management system. Many of the species/genotypes identified have a zoonotic potential and might represent a public health concern. [ABSTRACT FROM AUTHOR]
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- 2022
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8. Molecular survey of Besnoitia spp. (Apicomplexa) in faeces from European wild mesocarnivores in Spain.
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González‐Barrio, David, Köster, Pamela C., Habela, Miguel A., Martín‐Pérez, Manuel, Fernández‐García, Jose L., Balseiro, Ana, Barral, Marta, Nájera, Fernando, Figueiredo, Ana M., Palacios, Maria Jesus, Mateo, Marta, Carmena, David, Álvarez‐García, Gema, and Calero‐Bernal, Rafael
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OMNIVORES ,RED fox ,APICOMPLEXA ,CANIDAE ,FELIDAE ,FECES - Abstract
Numerous studies have unsuccessfully tried to unravel the definitive host of the coccidian parasite Besnoitia besnoiti. Cattle infections by B. besnoiti cause a chronic and debilitating condition called bovine besnoitiosis that has emerged in Europe during the last two decades, mainly due to limitations in its control associated with the absence of vaccines and therapeutical tools. Although the exact transmission pathways of B. besnoiti is currently unknown, it is assumed that the parasite might have an indirect life cycle with a carnivore as definitive host. Current lack of studies in wildlife might underestimate the importance of free‐living species in the epidemiology of B. besnoiti. Thus, the aim of the present study is to assess the presence of Besnoitia spp. in free‐ranging mesocarnivores in Spain. DNA was searched by PCR on faeces collected from wild carnivores as a first approach to determine which species could be considered as potential definitive host candidates in further research. For this purpose, a total of 352 faecal samples from 12 free‐living wild carnivore species belonging to the Canidae, Felidae, Herpestidae, Mustelidae, Procyonidae and Viverridae families were collected in seven Spanish regions. PCR testing showed that Besnoitia spp. DNA was present in four faecal samples from red foxes collected in western Spain, an area with the greatest density of extensively reared cattle and associated with high incidence of bovine besnoitiosis in the country. To date, this is the first report of a B. besnoiti‐like sequence (99.57% homology) from carnivore faeces in a worldwide context. Red foxes might contribute to the epidemiology of B. besnoiti, although further studies, mostly based on bioassay, would be needed to elucidate the accuracy and extent of these interesting findings. [ABSTRACT FROM AUTHOR]
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- 2021
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9. Molecular Detection and Characterization of Blastocystis sp. and Enterocytozoon bieneusi in Cattle in Northern Spain.
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Abarca, Nadia, Santín, Mónica, Ortega, Sheila, Maloney, Jenny G., George, Nadja S., Molokin, Aleksey, Cardona, Guillermo A., Dashti, Alejandro, Köster, Pamela C., Bailo, Begoña, Hernández-de-Mingo, Marta, Muadica, Aly S., Calero-Bernal, Rafael, Carmena, David, and González-Barrio, David
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BLASTOCYSTIS ,PARASITES ,RIBOSOMAL RNA ,DNA sequencing ,ZOONOSES - Abstract
Some enteric parasites causing zoonotic diseases in livestock have been poorly studied or even neglected. This is the case in stramenopile Blastocystis sp. and the microsporidia Enterocytozoon bieneusi in Spain. This transversal molecular epidemiological survey aims to estimate the prevalence and molecular diversity of Blastocystis sp. and E. bieneusi in cattle faecal samples (n = 336) in the province of Álava, Northern Spain. Initial detection of Blastocystis and E. bieneusi was carried out by polymerase chain reaction (PCR) and Sanger sequencing of the small subunit (ssu) rRNA gene and internal transcribed spacer (ITS) region, respectively. Intra-host Blastocystis subtype diversity was further investigated by next generation amplicon sequencing (NGS) of the ssu rRNA gene in those samples that tested positive by conventional PCR. Amplicons compatible with Blastocystis sp. and E. bieneusi were observed in 32.1% (108/336, 95% CI: 27.2-37.4%) and 0.6% (2/336, 95% CI: 0.0-1.4%) of the cattle faecal samples examined, respectively. Sanger sequencing produced ambiguous/unreadable sequence data for most of the Blastocystis isolates sequenced. NGS allowed the identification of 10 Blastocystis subtypes including ST1, ST3, ST5, ST10, ST14, ST21, ST23, ST24, ST25, and ST26. All Blastocystis-positive isolates involved mixed infections of 2-8 STs in a total of 31 different combinations. The two E. bieneusi sequences were confirmed as potentially zoonotic genotype BEB4. Our data demonstrate that Blastocystis mixed subtype infections are extremely frequent in cattle in the study area. NGS was particularly suited to discern underrepresented subtypes or mixed subtype infections that were undetectable or unreadable by Sanger sequencing. The presence of zoonotic Blastocystis ST1, ST3, and ST5, and E. bieneusi BEB4 suggest cross-species transmission and a potential risk of human infection/colonization. [ABSTRACT FROM AUTHOR]
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- 2021
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10. Enterocytozoon bieneusi (Microsporidia): Identification of novel genotypes and evidence of transmission between sympatric wild boars (Sus scrofa ferus) and Iberian pigs (Sus scrofa domesticus) in Southern Spain.
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Dashti, Alejandro, Rivero‐Juarez, Antonio, Santín, Mónica, López‐López, Pedro, Caballero‐Gómez, Javier, Frías‐Casas, Mario, Köster, Pamela C., Bailo, Begoña, Calero‐Bernal, Rafael, Briz, Verónica, and Carmena, David
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WILD boar ,SWINE ,MICROSPORIDIOSIS ,MICROSPORIDIA ,GENOTYPES ,ENTEROCYTOZOON bieneusi - Abstract
Microsporidia is a phylum of obligate emergent intracellular protist‐like fungi pathogens that infect a broad range of hosts including vertebrates and invertebrates. Enterocytozoon bieneusi is the most common cause of microsporidiosis in humans, affecting primarily immunosuppressed patients but also reported in immunocompetent individuals. Epidemiological information on the presence and molecular diversity of E. bieneusi in livestock and wildlife in Spain is limited. Therefore, the occurrence of this microsporidia was investigated in sympatric extensively reared Iberian pigs (n = 186) and free ranging wild boars (n = 142) in the province of Córdoba, Southern Spain. Forty‐two Iberian pigs (22.6%) and three wild boars (2.1%) were found E. bieneusi positive by PCR. In Iberian pigs, occurrence of E. bieneusi was significantly higher in sows than in fattening pigs (31.6% vs. 11.4%; p =.001). Five genotypes were identified in Iberian pigs, four previously reported (EbpA, PigEb4, O, Pig HN‐II) and a novel genotype (named PigSpEb1), while only two genotypes were identified in wild boars, EbpA and novel genotype PigSpEb1. All five genotypes identified belong to Group 1 suggesting zoonotic potential. This study constitutes the first report on the occurrence and molecular characterization of E. bieneusi in Iberian pigs and wild boars. The identification of two genotypes with zoonotic potential in sympatric Iberian pigs and wild boars suggests that E. bieneusi can be potentially transmitted between those two hosts, but also implies that they may act as natural sources of microsporidia infection to other hosts including humans. [ABSTRACT FROM AUTHOR]
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- 2020
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11. Survey of Crimean-Congo Hemorrhagic Fever Enzootic Focus, Spain, 2011-2015.
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Negredo, Ana, Habela, Miguel Ángel, de Arellano, Eva Ramírez, Diez, Francisco, Lasala, Fátima, López, Pablo, Sarriá, Ana, Labiod, Nuria, Calero-Bernal, Rafael, Arenas, Miguel, Tenorio, Antonio, Estrada-Peña, Agustín, Sánchez-Seco, Maria Paz, and Ramírez de Arellano, Eva
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HEMORRHAGIC fever ,NON-coding RNA ,ANAPLASMA phagocytophilum ,HYALOMMA ,RNA viruses ,TICKS - Abstract
During 2011-2015, we conducted a Crimean-Congo hemorrhagic fever virus (CCHFV) survey in captured ticks that were feeding mainly on wild and domestic ungulates in Spain, where presence of this virus had been reported previously. We detected CCHFV RNA in Hyalomma lusitanicum and H. marginatum ticks for 3 of the 5 years. The rate of infected ticks was 2.78% (44/1,579), which was similar to those for other countries in Europe with endemic foci for CCHFV (Kosovo, Bulgaria, and Albania). These data confirm the established spread of CCHFV into western Europe. Phylogenetic study of the small RNA segment showed Africa-3 clade as the only genotype identified, although we observed cocirculation of genetic variants during 2011 and 2015. We could not rule out genetic reassortments because of lack of sequence data for the medium and large RNA segments of the virus genome. [ABSTRACT FROM AUTHOR]
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- 2019
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12. The mosquito fauna of the western region of Spain with emphasis on ecological factors and the characterization of Culex pipiens forms.
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Bravo-Barriga, Daniel, Gomes, Bruno, Almeida, Antonio P. G., Serrano-Aguilera, Francisco J., Pérez-Martín, Juan E., Calero-Bernal, Rafael, Reina, David, Frontera, Eva, and Pinto, João
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This study updates the diversity, distribution, and seasonal trends of mosquitoes in a western region of Spain, assesses ecological determinants of Culex pipiens s.l., and determines form composition of Cx. pipiens s.s. populations. A total of 1,495 mosquitoes of 16 species was collected during 2012-2013, of which Cx. pipiens s.l. and Cx. theileri were the most abundant. Five new records for An. maculipennis s.s., Orthopodomyia pulcripalpis, Aedes (Ochlerotatus) punctor, Cx. europaeus, and Cx. modestus were found for this region. Cx. pipiens density varied across weather and habitat patterns, correlating positively with high temperatures and with a preference for urbanized areas and rural areas within a proximity of ovine farms. Moreover, molecular identification by CQ11FL was performed in 467 Cx. pipiens s.s., detecting both pipiens (66%) and molestus (8.4%) forms coexisting in different habitats (urban, peri-urban and rural) aboveground with a high degree of hybridization (25.7%). The abundance of Cx. pipiens in urban areas and farms, with the presence of hybrids, may increase their capacity to act as bridge vectors for the transmission of arboviral infections. These data will be helpful for further implementation of entomological programs focused on risk assessment for arboviruses or other mosquito-borne pathogens. [ABSTRACT FROM AUTHOR]
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- 2017
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13. Prevalence and Genotype Identification of Toxoplasma gondii in Wild Animals from Southwestern Spain.
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Calero-Bernal, Rafael, Saugar, José M., Frontera, Eva, Pérez-Martín, Juan E., Habela, Miguel A., Serrano, Francisco J., Reina, David, and Fuentes, Isabel
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The article reports on the prevalence of Toxoplasma gondii among wild animals in the southwestern area of Spain. Topics discussed include the use of polymerase chain reaction (PCR) in detecting the zoonotic pathogen in wild animals, the animals that tested positive for T. gondii and the implication of the genetic variability produced by the test results for domestic animal and human infection.
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- 2015
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14. Resolution of canine ocular thelaziosis in avermectin-sensitive Border Collies from Spain.
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Calero-Bernal, Rafael, Sánchez-Murillo, José Marín, Alarcón-Elbal, Pedro María, Sánchez-Moro, José, Latrofa, Maria Stefanía, Dantas-Torres, Filipe, and Otranto, Domenico
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AVERMECTINS , *ANTIPARASITIC agents , *DOG parasites , *EYE infections , *BORDER collie - Abstract
Abstract: Ocular thelaziosis by Thelazia callipaeda is an emerging disease that affects primarily dogs, but also cats, foxes and other wild carnivores, as well as humans. Three clinical cases of unilateral conjunctivitis caused by Thelazia nematodes were detected in Border Collie, a dog breed intolerant to the macrocyclic lactones. Animals came from southwestern Spain, on the border with Portugal. Eight worms were collected and identified molecularly as T. callipaeda by amplification and sequencing of partial cytochrome c oxidase subunit 1 gene. Oral treatment with mebendazole 20mg/kg (Telmin®) was effective in curing the infection. [Copyright &y& Elsevier]
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- 2014
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15. First Description of Sarcoptic Mange in a Free-Ranging European Wildcat (Felis silvestris silvestris) from Spain.
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Nájera, Fernando, Crespo, Elena, García-Talens, Amalia, Grande-Gómez, Rebeca, Herrera-Sánchez, Francisco Javier, Gentil, Michaela, Cortés-García, Carmen, Müller, Elisabeth, Calero-Bernal, Rafael, and Revuelta, Luis
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FELIS ,WILDLIFE conservation ,SARCOPTES scabiei ,MITE infestations ,FELIDAE ,DEMODEX - Abstract
Simple Summary: Sarcoptic mange caused by the mite Sarcoptes scabiei is a worldwide-distributed skin infestation with a wide range of hosts, among them several species within the Felidae family. Sarcoptes scabiei was diagnosed in a dead adult female European wildcat (Felis silvestris silvestris) from Spain. This is the first description of Sarcoptes scabiei in a European wildcat. Since this is a species of conservation concern due to its critical demography in the southernmost population of the Iberian Peninsula, the impacts of infectious diseases, including sarcoptic mange, should be considered during disease surveillance programs of the species' populations. Sarcoptic mange caused by the mite Sarcoptes scabiei is a worldwide-distributed skin infestation with a wide range of hosts, among them several species within the Felidae family. Sarcoptes scabiei was diagnosed in a dead adult female European wildcat (Felis silvestris silvestris) from Spain, based on histological evaluation of skin biopsies and identification of the arthropod from skin scrapings and molecular methods. This is the first description of Sarcoptes scabiei in a European wildcat. Due to its critical demography in the southernmost population of the Iberian Peninsula, the impacts of infectious diseases, including sarcoptic mange, as a new potential threat should be considered during disease surveillance programs of the species' populations. [ABSTRACT FROM AUTHOR]
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- 2021
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16. Severe beak deformity in Melopsittacus undulatus caused by Knemidocoptes pilae.
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ELBAL, Pedro María ALARCÓN, SALIDO, Víctor Jesús CARMONA, SÁNCHEZ-MURILLO, José Marín, CALERO BERNAL, Rafael, and LUCIENTES CURDI, Javier
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BUDGERIGAR ,EPIDEMICS ,MITES as carriers of disease ,MELOPSITTACUS - Abstract
An outbreak of knemidocoptic mange caused by Knemidocoptes pilae is described in caged Australian budgerigars (Melopsittacus undulatus) in a public park in L'Eliana (Valencia, Spain). Five individuals (5 out of 70) displayed severe malformation of the beak, with the rhamphotheca, rhinotheca, and gnathotheca affected and a slight lateral deviation of the jawbone, which did not, however, impede feeding or grooming. [ABSTRACT FROM AUTHOR]
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- 2014
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17. Absence of Neospora caninum DNA in Human Clinical Samples, Spain.
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Calero-Bernal, Rafael, Horcajo, Pilar, Hernández, Marta, Ortega-Mora, Luis Miguel, and Fuentes, Isabel
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NEOSPORA caninum , *HUMAN DNA , *ANTIBODY titer , *TOXOPLASMOSIS , *SIMULATED patients , *COCCIDIOSIS , *COMPARATIVE studies , *DNA , *RESEARCH methodology , *MEDICAL cooperation , *POLYMERASE chain reaction , *PROTOZOA , *PUBLIC health surveillance , *RESEARCH , *EVALUATION research - Abstract
Low antibody titers to Neospora caninum have been reported in humans, but infection has not been confirmed. We used N. caninum-specific PCR to test 600 clinical samples from patients with toxoplasmosis signs but Toxoplasma gondii-negative PCR results. We did not detect N. caninum DNA, demonstrating it is an unlikely opportunistic zoonotic agent. [ABSTRACT FROM AUTHOR]
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- 2019
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18. Occurrence and molecular genotyping of Giardia duodenalis and Cryptosporidium spp. in wild mesocarnivores in Spain.
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Mateo, Marta, de Mingo, Marta Hernández, de Lucio, Aida, Morales, Lucía, Balseiro, Ana, Espí, Alberto, Barral, Marta, Lima Barbero, José Francisco, Habela, Miguel Ángel, Fernández-García, José L., Bernal, Rafael Calero, Köster, Pamela C., Cardona, Guillermo A., and Carmena, David
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GIARDIA , *CRYPTOSPORIDIUM , *GLUTAMATE dehydrogenase , *EPIDEMIOLOGY - Abstract
There is a surprisingly scarce amount of epidemiological and molecular data on the prevalence, frequency, and diversity of the intestinal protozoan parasites Giardia duodenalis and Cryptosporidium spp. in wildlife in general and mesocarnivore species in particular. Consequently, the extent of the cyst/oocyst environmental contamination attributable to these wild host species and their potential implications for public veterinary health remain largely unknown. In this molecular epidemiological survey a total of 193 individual faecal samples from badgers ( Meles meles , n = 70), ferrets ( Mustela putorius furo , n = 2), genets ( Genetta genetta , n = 6), Iberian lynxes ( Lynx pardinus , n = 6), beech martens ( Martes foina , n = 8), mongooses ( Herpestes ichneumon , n = 2), otters ( Lutra lutra , n = 2), polecats ( Mustela putorius , n = 2), red foxes ( Vulpes vulpes , n = 87), wildcats ( Felis silvestris , n = 2), and wolves ( Canis lupus , n = 6) were obtained from road-killed, hunted, and accidentally found carcasses, and from camera-trap surveys or animals entering rescue shelters, during the period December 2003–April 2016. Investigated specimens were collected in five Spanish autonomous regions including Andalusia ( n = 1), Asturias ( n = 69), Basque Country ( n = 49), Castile-La Mancha ( n = 38), and Extremadura ( n = 36). The presence of cysts/oocysts was confirmed by PCR-based methods targeting the small subunit ( ssu ) ribosomal RNA gene of these parasite species. Genotyping of the obtained isolates were attempted at appropriate markers including the glutamate dehydrogenase ( G. duodenalis ) and the 60-kDa glycoprotein ( C. parvum and C. ubiquitum ) loci. Overall, G. duodenalis was detected in 8% (7/87) of red foxes, a single beech marten, and a single wolf, respectively. Cryptosporidium was identified in 3% (2/70) of badgers, 8% (7/87) of red foxes, a single genet, and a single mongoose, respectively. None of the nine G. duodenalis isolates generated could be genotyped at the assemblage/sub-assemblage level. Out of the nine Cryptosporidium isolates successfully characterized, three were identified as C. canis (one in a mongoose and two in red foxes), and three as C. parvum (one in a badger and three in red foxes). The remaining three isolates were assigned to C. felis (in a red fox), C. hominis (in a badger), and C. ubiquitum (in a red fox), respectively. Two additional Cryptosporidium isolates infecting a badger and a genet, respectively, were untypable. The red fox was confirmed as a suitable host of potentially zoonotic Cryptosporidium species, mainly C. parvum and C. ubiquitum . The high mobility and wide home range of red foxes, together with their increasing presence in urban and peri-urban settings, may led to the overlapping of sylvatic and domestic cycles of the parasite, and consequently, to an increased risk of cryptosporidiosis in production animals and humans. The detection of C. hominis oocysts in a badger raises the question of whether this finding represents a true infection or a sporadic event of mechanical passage of C. hominis oocyst of anthroponotic origin. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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