1. A Retrievable and Highly Selective Fluorescent Probe for Monitoring Sulfide and Imaging in Living Cells
- Author
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Feng-juan Cheng, Decheng Bai, Ju Cheng, Guoqiang Xie, Fengping Hou, Pinxian Xi, Xiaojun Yao, Yanjun Shi, Xuefei Zhao, Zhengzhi Zeng, and Liang Huang
- Subjects
Models, Molecular ,inorganic chemicals ,Sulfide ,Cell Survival ,Inorganic chemistry ,Sulfides ,Mass spectrometry ,Photochemistry ,Cell Line ,Ion ,Inorganic Chemistry ,Mice ,chemistry.chemical_compound ,Animals ,Humans ,Physical and Theoretical Chemistry ,Sulfate ,Fluorescein ,Fluorescent Dyes ,chemistry.chemical_classification ,Fibroblasts ,Oxyquinoline ,Highly selective ,Fluorescence ,Spectrometry, Fluorescence ,Microscopy, Fluorescence ,chemistry ,Copper ,Derivative (chemistry) ,HeLa Cells - Abstract
A novel selective fluorescent chemosensor based on an 8-hydroxyquinoline-appended fluorescein derivative (L1) was synthesized and characterized. Once combined with Cu(2+), it displayed high specificity for sulfide anion. Among the various anions, only sulfide anion induced the revival of fluoresecence of L1, which was quenched by Cu(2+), resulting in "off-on"-type sensing of sulfide anion. What's more, the sensor was retrievable to indicate sulfide anions with Cu(2+), and S(2-), in turn, increased. With the addition of Cu(2+), compound L1 could give rise to a visible pink-to-yellow color change and green fluorescence quenching. The resulting yellow solution could change to pink and regenerate to green fluorescence immediately upon the addition of sulfide anion; however, no changes were observed in the presence of other anions, including CN(-), P(2)O(7)(4-), and other forms of sulfate, making compound L1 an extremely selective and efficient sulfide chemosensor. The signal transduction occurs via reversible formation-separation of complex L1Cu and CuS. What's more, the biological imaging study has demonstrated that the chemosensor can detect sulfur anions in biological systems at a relatively low concentration.
- Published
- 2012