1. LaPSvS1, a (1--3)-beta-galactan sulfate and its effect on angiogenesis in vivo and in vitro
- Author
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H. Vogl, Robert J. Linhardt, Gerhard Franz, Jutta Bürgermeister, and D. H. Paper
- Subjects
Time Factors ,Angiogenesis ,Anti-Inflammatory Agents ,Neovascularization, Physiologic ,Angiogenesis Inhibitors ,Chick Embryo ,Sulfuric Acid Esters ,Fibroblast growth factor ,Biochemistry ,Galactans ,Analytical Chemistry ,chemistry.chemical_compound ,Sulfation ,In vivo ,Allantois ,Animals ,Sodium dodecyl sulfate ,Plants, Medicinal ,Dose-Response Relationship, Drug ,Chemistry ,Heparin ,Sulfates ,Organic Chemistry ,Sodium Dodecyl Sulfate ,Fabaceae ,General Medicine ,Chorion ,Galactan ,Arabinose ,In vitro ,Angiogenesis inhibitor ,Spectrometry, Fluorescence ,Fibroblast Growth Factor 2 - Abstract
LaPSvS1, a highly sulfated branched (1-->3)-beta-galactan was prepared from the arabino-galactan from Larix decidua Miller by partial hydrolysis and subsequent sulfation with SO(3)-pyridine in DMF. The molecular weight was analyzed by GPC and the sulfate content was determined by ion chromatography. LaPSvS1 exhibited good antiangiogenic and antiinflammatory effects in two different modifications of the known CAM-assay. In vitro results obtained in the FGF-2-trypsin-assay and in fluorospectrometric experiments revealed that LaPSvS1 interacts with the fibroblast growth factor 2 system. This interaction is correlated with the in vivo effect of LaPSvS1 on FGF-2 induced angiogenesis.
- Published
- 2002