28 results on '"Bursa of Fabricius cytology"'
Search Results
2. Na+/H+ exchanger 1 gene expression in tissues of yellow chicken.
- Author
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Ning ZY, An YF, Qi WB, Wang H, Pan JQ, Wu XT, and Liao M
- Subjects
- Animals, Avian Proteins metabolism, Bursa of Fabricius cytology, Chickens metabolism, Gene Expression, Organ Specificity, RNA, Messenger genetics, RNA, Messenger metabolism, Sodium-Hydrogen Exchangers metabolism, Spleen cytology, Avian Proteins genetics, Bursa of Fabricius metabolism, Chickens genetics, Sodium-Hydrogen Exchangers genetics, Spleen metabolism
- Abstract
The Na(+)/H(+) exchanger 1 (NHE1) transmembrane protein regulates intracellular pH, cell survival, cell growth, cell differentiation and plays a critical role in the progression of some diseases, including the pathogenesis of J avian leukosis. The chicken is an ideal model to study the function of NHE1 because it has developed highly efficient Na(+)-absorptive mechanisms in its small and large intestines. To date, there has been no detailed expression analysis to determine NHE1 expression in various tissues of the chicken. We determined the mRNA and protein expression levels of avian NHE1 by real-time quantitative PCR and immunohistochemical analysis. NHE1 mRNA was detected in all chicken tissues examined. Protein expression levels varied widely among tissues and did not always correlate with mRNA expression. Determining the mRNA and protein of NHE1 expression patterns in chicken should help to delineate the NHE1 role in different tissues and its contribution to physiological and pathological processes. These data provide the basis for examining the distinct function of chicken NHE1 compared with its mammalian counterpart.
- Published
- 2012
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3. Chicken chemokine receptors in T cells isolated from lymphoid organs and in splenocytes cultured with concanavalin A.
- Author
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Annamalai T and Selvaraj RK
- Subjects
- Animals, Bursa of Fabricius cytology, Bursa of Fabricius drug effects, Bursa of Fabricius immunology, CD4-Positive T-Lymphocytes cytology, CD8-Positive T-Lymphocytes cytology, Cecum cytology, Cecum drug effects, Cecum immunology, Concanavalin A pharmacology, DNA Primers, Palatine Tonsil cytology, Palatine Tonsil drug effects, Palatine Tonsil immunology, Polymerase Chain Reaction methods, Receptors, Chemokine genetics, Spleen cytology, Spleen drug effects, Thymus Gland cytology, Thymus Gland drug effects, Thymus Gland immunology, CD4-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes immunology, Chickens immunology, Receptors, Chemokine immunology, Spleen immunology
- Abstract
Chemokine receptors guide immune cells to specific organs during health and disease. The mRNA content of the chemokine receptors CCR2, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CXCR4, CXCR5, and CX3CR1 in CD4(+) cells (T-helper cells) isolated from blood, bursa, cecal tonsil, spleen, and thymus and in CD8(+) cells (T-cytotoxic cells) isolated from blood, cecal tonsil, spleen, and thymus were investigated. The CD4(+) cells isolated from thymus had the highest amount of CCR7 and CCR8 mRNA. The CD4(+) cells isolated from bursa, cecal tonsil, and thymus had the highest amount of CCR5 mRNA. The CD4(+) cells isolated from cecal tonsils had the highest amount of CCR9 mRNA. The CD4(+) cells isolated from bursa and thymus had the highest amount of CXCR5 mRNA. The CD8(+) cells isolated from cecal tonsil had the highest mRNA amount of all receptors studied except CCR9 and CX3CR1. The CD4(+) cells treated with concanavalin A had increased CCR2, CCR4, CCR7, CCR8, and CXCR5 mRNA amounts at 24 h of stimulation. The CD8(+) cells treated with concanavalin A had increased CCR4 mRNA at 72 h, increased CCR6 mRNA at 24 h, and decreased CCR8 and CXCR4 mRNA at 24 h of stimulation.
- Published
- 2010
- Full Text
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4. B cell differentiation in the bursa of Fabricius and spleen of embryos and chicks immediately after hatching.
- Author
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Narabara K, Abe A, Hanieh H, and Kondo Y
- Subjects
- Animals, Flow Cytometry, Immunohistochemistry, B-Lymphocytes cytology, Bursa of Fabricius cytology, Cell Differentiation physiology, Chickens immunology, Spleen cytology
- Abstract
Flow cytometric analysis and immunohistochemical observation were used to qualitatively and quantitatively clarify the nature of B cell differentiation in the bursa of Fabricius of chick embryos and to determine the timing of antibody class switching in chicken spleens based on positivity of IgM and IgG on and in the cells. In the bursa, the sIgM-positive cell population formed from the 12(th) to 15(th) day of embryogenesis. The proportion of sIgM-high expressing (sIgM(high)) cells was lower among bursacytes than splenocytes of hatched chicks, suggesting that the sIgM(high) bursacytes are to be released to peripheral sites. The proportion of sIgM(high) cells was higher at 0 days old than at any other examined stage of development. Colonization of the spleen by B cells occurred between the 18(th) day of embryogenesis and 0 days old. Antibody class switching was thought to start in the spleen between 1 and 2 weeks of age, because IgG-positive cells were present in the spleen of 2-week-old chicks, but not 0-day-old or 1-week-old chicks.
- Published
- 2009
- Full Text
- View/download PDF
5. Mobiloization of intracellular calcium ions in chicken and rat lymphocytes induced by T cell mitogens.
- Author
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Gerilechaogetu, Narahara K, Abe A, and Kondo Y
- Subjects
- Animals, Bursa of Fabricius cytology, Bursa of Fabricius drug effects, Chickens, Cytoplasm metabolism, Extracellular Space metabolism, Mitogens pharmacology, Rats, Species Specificity, Spleen cytology, Spleen drug effects, T-Lymphocytes drug effects, T-Lymphocytes immunology, Thymus Gland cytology, Thymus Gland drug effects, Bursa of Fabricius metabolism, Calcium metabolism, Concanavalin A pharmacology, Phytohemagglutinins pharmacology, Spleen metabolism, T-Lymphocytes metabolism, Thymus Gland metabolism
- Abstract
Cytosolic Ca(2+) is known to be an important factor in intracellular signaling pathways that regulate several cellular functions. The present study was designed to measure the intracellular concentrations of Ca(2+) ([Ca(2+)](i)) in T cell mitogen-stimulated chicken lymphocytes, and to compare the results with those in rat lymphocytes. [Ca(2+)](i) was increased in the thymocytes, splenocytes and bursacytes of chickens, and in the thymocytes and splenocytes of rats following exposure to the mitogens phytohaemagglutinin (PHA) and concanavalin A (ConA). Increases were greatest in the thymocytes followed by the splenocytes and bursacytes. The PHA-induced changes in the thymocytes and splenocytes were similar in chickens and rats, but the ConA-induced increases were significantly lower in the chickens than rats. Pretreatment with EGTA before the application of PHA and ConA completely suppressed the rise in [Ca(2+)](i) in all the chicken lymphocytes, indicating that the increases that occurred in PHA- and ConA-treated chicken lymphocytes could be entirely attributed to the influx of extracellular Ca(2+). On the other hand, the PHA- and ConA-induced increase in [Ca(2+)](i) in rat lymphocytes was not completely suppressed by EGTA, indicating the recruitment of Ca(2+) from the intracellular Ca(2+) pool. The results suggest species differences in the Ca(2+)-based responses to T cell mitogens between chicken lymphocytes and rat lymphocytes.
- Published
- 2009
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6. Age-related changes in the number of mast cells in the avian lymphoid organs.
- Author
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Karaca T, Yörük M, and Uslu S
- Subjects
- Aging physiology, Animals, Chickens growth & development, Bursa of Fabricius cytology, Chickens anatomy & histology, Mast Cells cytology, Spleen cytology, Thymus Gland cytology
- Abstract
The distribution of mast cells (MCs) was studied in the lymphoid organs (thymus, bursa of Fabricius and spleen) of 0-, 7-, 21-, 30- and 120-day-old chickens, using light microscopic histochemical techniques. Tissues samples were obtained under deep anaesthesia from animals in five groups. Tissues were fixed in Mota's fixative (basic lead acetate) for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with toluidine blue in 0.5% aqueous solution at pH 1.0 for 5 min and Alcian blue/Safranine at pH 1.42 for 30 min. MCs were found in the organs, mostly associated with sinuses and blood vessels. A large increase in MCs was observed in both thymus and spleen of 21-day-old chickens compared with 0-, 7-, 30- and 120-day-old chickens. However, in the bursa of Fabricius, numbers of MCs were significantly higher in the 7-day-old group compared with other age groups. Safranine-positive MCs were not observed in all organs and age groups. These results showed age-related changes in the number of MCs in avian lymphoid tissues.
- Published
- 2006
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7. Peripheral blood fibrocytes contribute to the formation of the avian spleen.
- Author
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Nagy N, Bíró E, Takács A, Pólos M, Magyar A, and Oláh I
- Subjects
- Animals, Antigens, Differentiation, B-Lymphocyte biosynthesis, Cell Differentiation, Cell Transplantation, Chick Embryo cytology, Chimera, Collagen metabolism, Collagen Type I biosynthesis, Collagen Type III biosynthesis, Collagen Type III metabolism, Connective Tissue Cells cytology, Coturnix, Extracellular Matrix metabolism, Hematopoiesis, Hematopoietic Stem Cells cytology, Histocompatibility Antigens Class II biosynthesis, Immunohistochemistry, Leukocyte Common Antigens biosynthesis, Macrophages cytology, Macrophages metabolism, Microscopy, Fluorescence, Models, Anatomic, Models, Biological, Monocytes cytology, Phagocytosis, Quail, Recombinant Fusion Proteins metabolism, Time Factors, Blood Cells cytology, Bursa of Fabricius cytology, Embryonic Development, Gene Expression Regulation, Developmental, Spleen cytology, Spleen embryology
- Abstract
Chick-quail chimeric studies were made to determine the origin of the cells of splenic ellipsoid. The ellipsoid is formed by supporting and phagocytic cells, which are embedded in a well-organized extracellular matrix. Splenic and bursal anlage of 6- to 6.5-day-old quail embryos were transplanted into the coelomic cavity of 3-day-old chick embryos and further incubated for 17 days. CD45+ chicken hemopoietic cells colonized both organs. They formed the cells of the ellipsoid and the periellipsoidal white pulp of the transplanted quail spleen. Chicken-specific collagen III was produced only in the donor quail spleen, but not in the bursa of Fabricius. The CD45+/collagen I+/collagen III+ cells are probably identical with the mammalian peripheral blood fibrocytes and contribute to the formation of supporting cells, whereas the CD45+/74.2+ ellipsoid-associated macrophages are of monocytic origin. We provide, for the first time, experimental evidence that peripheral blood fibrocytes exist in the avian species; they are present in the circulation of the chicken embryo and contribute to the organogenesis of the spleen.
- Published
- 2005
- Full Text
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8. In vitro characterization of chB6 positive and negative cells from early avian embryos.
- Author
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Weber WT
- Subjects
- Animals, Cell Lineage, Cell Separation, Cells, Cultured, Phorbol 12,13-Dibutyrate pharmacology, Bone Marrow Cells, Bursa of Fabricius cytology, Chick Embryo cytology, Hematopoietic Stem Cells, Isoantigens, Lymphocytes immunology, Spleen cytology
- Abstract
Density gradient-separated embryonic spleen and bone marrow cells were enriched for chB6(+) cells with positive and negative selection procedures and magnetic cell sorting. The majority of chB6(+) cells, consisting of small, dense, strongly chB6(+) cells, were prone to apoptosis, which was further accentuated after exposure to monoclonal antibodies directed against chB6 alloantigen, but was largely inhibited by PDBU, leading to maintenance and frequently numerical increases of chB6(+) cells after an initial decline. sIgM(+) cells within that population followed a very similar pattern, suggesting a PDBU-induced upregulation of sIgM expression on a proportion of chB6(+) cells. The protective effect of PDBU on anti-chB6-exposed cells was confirmed with bursal lymphocytes and shown to be entirely PDBU concentration dependent. It was calculated that each 14-day embryonic spleen contained a minimum of 250,000 chB6(+) and 3000-4000 sIgM(+) cells, respectively. Endogenous apoptosis appeared to be increased with embryonic age, reaching a peak with bursal cells in the posthatching period. A second population of larger, less dense, and weak chB6(+) cells, often with vacuoles in a more abundant cytoplasm, differed functionally, expanding numerically in unstimulated cultures and being inhibited by PDBU. No sIgM(+) cells developed within this population. It is proposed that this chB6(+) fraction may represent progenitors of a previously suggested chB6(+) subset of macrophages, in contrast to the dense chB6(+), small cells, viewed as B cell progenitors. chB6(-) cells, consisting predominantly of granulocytes, proliferated vigorously in unstimulated cultures, but were consistently inhibited by PDBU. Coculture of age-matched embryonic bursal stroma with positively and negatively enriched chB6(+) cells revealed enhanced protection from apoptosis for chB6(+) cells and a PDBU-induced upregulation of sIgM expressing cells. Bursal stroma also had a pronounced positive effect on the proliferation of chB6(-) cells., (Copyright 2000 Academic Press.)
- Published
- 2000
- Full Text
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9. Characterization of a novel monoclonal antibody, EIV-E12, raised against enriched splenic ellipsoid-associated cells.
- Author
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Pharr T, Olah I, Bricker J, Olson WC, Ewert D, Marsh J, and Glick B
- Subjects
- Animals, Antigens, Surface chemistry, Antigens, Surface isolation & purification, B-Lymphocytes cytology, B-Lymphocytes immunology, Bursa of Fabricius cytology, Bursa of Fabricius immunology, Chick Embryo, Chickens, Dendritic Cells cytology, Dendritic Cells immunology, Hybridomas immunology, Immunohistochemistry, Lymphoid Tissue cytology, Lymphoid Tissue immunology, Mice, Molecular Weight, Precipitin Tests, Antibodies, Monoclonal, Spleen cytology, Spleen immunology
- Abstract
A monoclonal antibody, EIV-E12, was produced against ellipsoid-associated cells. In postnatal chicks, the antigen defined by EIV-E12 associated with about 90% of bursal cells and accumulated in B cell-rich areas of lymphoid organs. The antigen was expressed by many splenic cells on day 9 of embryogenesis and about 1 day later in the bursal anlage, where the cells positive for the antigen localized in close proximity to the plical epithelium. In the bursa, these early EIV-E12 cells appeared before Bu-1-positive cells and may represent a dendritic cell precursor. B cells within the developing bud acquired the EIV-E12 antigen. Immunoprecipitation studies revealed a molecular weight of 233 (nonreduced) and 205 (reduced) for the EIV-E12 antigen. These values were markedly different from the molecular weight of the antigen identified by Bu-1 but similar to that of a CB10 antigen. The CB10 and EIV-E12 MAbs exhibited some differences in cellular staining. Taken together these data suggest that EIV-E12 MAb recognizes a unique cellular population during embryogenesis.
- Published
- 1995
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10. The effect of anti-bursa serum (ABS) on the intensity of acid phosphatase reaction in bursa-dependent structures of the chicken spleen and on the level of antibodies in the blood serum.
- Author
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Graczyk S
- Subjects
- Acid Phosphatase analysis, Acid Phosphatase physiology, Agglutinins analysis, Animals, B-Lymphocytes cytology, B-Lymphocytes immunology, Bursa of Fabricius cytology, Bursa of Fabricius surgery, Chickens blood, Chickens metabolism, Erythrocytes immunology, Immune Sera immunology, Lymphatic System cytology, Lymphatic System immunology, Spleen cytology, Spleen immunology, T-Lymphocytes cytology, T-Lymphocytes immunology, Acid Phosphatase metabolism, Bursa of Fabricius immunology, Chickens immunology, Immune Sera pharmacology, Spleen enzymology
- Abstract
It has been found that administering rabbit anti-bursa serum (ABS) to chickens, like neonatal bursectomy, brings about a decreased acid phosphatase (APh) reaction in germinal centres type I and II, and in the periellipsoidal lymphatic tissue (PEL) of the spleen. It has turned out, that following a stimulation with sheep erythrocytes (SRBC) in the blood serum of the ABS-treated chickens appear traces of specific anti-SRBC agglutinins, while in bursectomized chickens there is only a slight decrease in the titre of those antibodies. The results may serve as a base for further studies on the role and participation of the spleen structures in chicken immunological response.
- Published
- 1994
11. Age-related changes of J-chain-positive cells in chickens.
- Author
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Moriya O and Ichikawa Y
- Subjects
- Aging, Animals, Bursa of Fabricius cytology, Bursa of Fabricius growth & development, Chick Embryo, Chickens, Fluorescent Antibody Technique, Immune Sera, Immunoglobulin mu-Chains analysis, Lymphocytes cytology, Lymphocytes immunology, Spleen cytology, Bursa of Fabricius immunology, Immunoglobulin J-Chains analysis, Immunoglobulin M analysis, Spleen immunology
- Abstract
Localizations of J-chain-positive cells (JPC) were examined in chicken lymphatic tissues before and after hatching. The cells containing J chain were first detected in medullary areas of the bursa of FABRICIUS during the embryonic stage. These positive cells were partly detected in the developing small lymphatic follicles: perhaps on newly differentiating precursor B-cells. In addition to these lymphatic follicles, connective tissue of bursal fold were also detected as J-chain positive. Although similar localizations of JPC were again observed in hatched chickens, positive areas of follicular medulla were strongly stained for fluorescence with corresponding antisera than that of embryonic ones. These data may reflect differences in the physiology of lymphocytes in respect to functional development. JPC localizations were next compared between the B-cell subpopulations, mu-(microPC) and alpha-chain-positive cells (alpha PC). The J-chains detectable in the IgM molecules were also found in follicular medulla. However, these follicles were almost found to be negative for J-chains detectable in the alpha PC before hatching. Any strong stainings for J-chain in the alpha PC were, moreover, not be observed in bursa after hatching. The microPC localizations in hatched chickens were roughly equal with the pattern of JPC localization. These analyses revealed the presence of the cells having the chains of both mu and J. The results together with other recent studies further shown that bursal J-chain can be partly detected in newly differentiated lymphatic follicles lacking IgM-producing and suggest the possible presence of B-cell-differentiation sequence of Ig-J+----IgM+J+----IgA+J+.
- Published
- 1991
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12. Production of an eosinophil migration factor in lymphocytes of mitogen-treated chick embryos.
- Author
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Moriya O and Ichikawa Y
- Subjects
- Animals, Chick Embryo physiology, Concanavalin A pharmacology, Eosinophils physiology, Lipopolysaccharides pharmacology, Lymphocytes drug effects, Lymphocytes embryology, Organ Specificity, Bursa of Fabricius cytology, Chemotactic Factors biosynthesis, Chemotactic Factors, Eosinophil biosynthesis, Lymphocytes metabolism, Mitogens pharmacology, Spleen cytology, Thymus Gland cytology
- Published
- 1981
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13. Flow cytometric analysis of bursal, thymic, and splenic cells from normal and cyclophosphamide-treated embryos.
- Author
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Glick B, LaVia MF, and Koger B
- Subjects
- Animals, Bursa of Fabricius embryology, Cell Cycle, Chick Embryo drug effects, Cyclophosphamide pharmacology, DNA analysis, Flow Cytometry, Lymphocytes analysis, Spleen embryology, Thymus Gland embryology, Bursa of Fabricius cytology, Chick Embryo cytology, Lymphocytes cytology, Spleen cytology, Thymus Gland cytology
- Abstract
Cell flow cytometry was used to assess the life cycle of embryonic lymphocytes. Cells were prepared for flow cytometry by fixation for 30 min in a final concentration of 70% ethyl alcohol, treated with ribonuclease (50 to 70 Kunitz/ml) and incubated for 30 min. in propidium iodide. Our data demonstrated that bursal lymphocytes from 20-day embryos (DE) exhibited significantly fewer cells in pre-DNA (deoxyribonucleic acid) and more cells in DNA synthesis (S) than thymic cells from 20 DE, and thymic lymphocytes from 16 DE expressed a more active S than bursal cells. Lymphocytes were separated from granulocytes by a Ficoll-hypaque double density gradient. The DNA cycle of bursal lymphocytes was more easily disrupted with cyclophosphamide (Cy) than the DNA cycle of thymic lymphocytes. However, unfractionated splenic cells from embryos treated with Cy revealed a greater percentage of cells in S and post-DNA synthesis and mitosis than splenic cells from control embryos. Because the splenic cells of Cy-treated embryos were predominantly immature granulocytes, it was concluded that Cy stimulated myelopoiesis in the spleen.
- Published
- 1985
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14. Diversity of natural haemolysin-producing cells in the developing chick embryo.
- Author
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Marković BD, Rajcević M, Isaković K, and Janković BD
- Subjects
- Animals, Antibody-Producing Cells, Bursa of Fabricius cytology, Chick Embryo cytology, Chickens, Guinea Pigs, Hemolytic Plaque Technique, Spleen cytology, Bursa of Fabricius metabolism, Chick Embryo immunology, Hemolysin Proteins biosynthesis, Spleen metabolism
- Published
- 1977
15. Bursal and postbursal cells in chicken: age-dependence of germinal center formation in spleen.
- Author
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Toivanen P, Toivanen A, Molnár G, and Sorvari T
- Subjects
- Age Factors, Animals, Antibody Formation, Brucella abortus immunology, Bursa of Fabricius cytology, Cell Differentiation, Chickens, Cyclophosphamide, Erythrocytes immunology, Immunosuppression Therapy, Lymphocyte Transfusion, Lymphoid Tissue cytology, Lymphoid Tissue immunology, Sheep immunology, Spleen cytology, Transplantation, Homologous, Bursa of Fabricius immunology, Spleen immunology
- Published
- 1974
- Full Text
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16. Comparison of lymphocyte populations bearing surface immunoglobulins in avian bone marrow, bursa, spleen and thymus.
- Author
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Glick B, Perkins WD, Rosse C, and Schwarz MR
- Subjects
- Agammaglobulinemia immunology, Age Factors, Animals, Autoradiography, Bursa of Fabricius immunology, Chickens, Cyclophosphamide, Immune Sera, Iodine Radioisotopes, Rabbits immunology, T-Lymphocytes immunology, Testosterone, gamma-Globulins, Binding Sites, Antibody, Bone Marrow immunology, Bone Marrow Cells, Bursa of Fabricius cytology, Lymphocytes immunology, Receptors, Antigen, B-Cell, Spleen cytology, Thymus Gland cytology
- Abstract
Rabbit anti-chicken gamma-globulin was labeled with 125I and then incubated with cells from the bursa, thymus, spleen, and bone marrow of 4- and 8-week old birds. The same procedure was carried out on 11-week-old agammaglobulinemic chickens. Autoradiography revealed that the majority of large, medium, and small bursal lymphocytes bind the antibodies while labeled lymphocytes of each type in the spleen and thymus never exceeded 11 or 4 percent, respectively. Labeled medium and small lymphocytes in the bone marrow increased from 4.2 and 1.7%, respectively, at 4 weeks of age, to 9.5 and 8.8%, respectively, at 8 weeks of age. Labeled lymphocytes of all sizes were completely absent in all tissues of agammaglobulinemic chicks, including the marrow. Therefore, the increase in frequency of labeled lymphocytes in the bone marrow with age may be a result of recruitment of cells from the bursa of Fabricius. The majority of lymphocytes in the bone marrow do not label. Therefore, lymphocytes from the bone marrow may be T cells, subsets of B cells, or neither T or B cells.
- Published
- 1975
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17. The bursa of Fabricius as a trapping mechanism for environmental antigens.
- Author
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Ekino S, Urano T, Fujii H, and Kotani M
- Subjects
- Age Factors, Agglutinins analysis, Animals, Antigens, Bacterial immunology, Body Weight, Bursa of Fabricius cytology, Bursa of Fabricius immunology, Cecum immunology, Chick Embryo, Chickens immunology, Spleen immunology, Bursa of Fabricius embryology, Spleen cytology
- Abstract
The bursa of Fabricius was isolated from gut-derived antigens by ligating the bursal duct on the 18th day of incubation. Ligation of the bursal duct (BDL) suppressed the bursal development, spontaneous germinal centre formation in the spleen and the development of serum "natural" agglutinins for bacteria or heteroerythrocytes. Moreover, administration of sterilized cecal contents into the bursal lumen at BDL led to normal level of serum "natural" agglutinins. These findings strongly suggest that the bursa of Fabricius possesses an antigen-dependent process which modulates the development of immune system. Antigen-trapping mechanism of the bursa may supply information about environmental antigens for this process.
- Published
- 1985
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18. Migratory patterns of B lymphocytes. III. Inhibition of splenic follicular localization of transferred chicken bursa cells by preincubation with anti-Ig-1.
- Author
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de Kruyff RH, Gilmour DG, and Thorbecke GJ
- Subjects
- Absorption, Adenosine metabolism, Animals, Autoradiography, Bursa of Fabricius cytology, Cell Movement, Chickens, Complement System Proteins, Guinea Pigs immunology, Immune Sera, Immunoglobulin Fab Fragments, Immunoglobulin G, Liver cytology, Lung cytology, Radiation Chimera, Thymus Gland cytology, Tritium, B-Lymphocytes immunology, Spleen cytology
- Abstract
Homing of 3-H-adenosine-labeled bursa cells to follicles in the chickens spleen was greatly reduced by prior incubation of the transferred cells with rabbit anti-chicken-immunoglobulin sera. This effect was transient, being present 6 hr after cell transfer but gone by 18 hr, when incubation was at 0 degreesC without complement. The inhibition persisted until at least 18 hr after incubation at 37 degrees C with complement.
- Published
- 1975
19. Developmental relationships between vitelline and intra-embryonic haemopoiesis studied in avian 'yolk sac chimaeras'.
- Author
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Martin C, Beaupain D, and Dieterlen-Lievre F
- Subjects
- Animals, Bone Marrow Cells, Bursa of Fabricius cytology, Chick Embryo, Coturnix, Hematopoiesis, Spleen cytology, Thymus Gland cytology, Bone Marrow embryology, Bursa of Fabricius embryology, Hematopoietic Stem Cells cytology, Spleen embryology, Thymus Gland embryology, Yolk Sac cytology
- Abstract
Using the quail-chick marker technique, cells have been traced in the haemopoietic organs of 149 developing chimaeras composed of a quail embryo with a chick yolk sac. The existence of intraembryonic stem cell relaying yolk sac stem cells, previously demonstrated with this system, is confirmed. Thymus, bursa of Fabricius and bone marrow are preferentially populated by intraembryonic stem cells. The spleen shows a transitory phase of colonization by chick yolk sac stem cells at 11--12 days of incubation. From 7 days onwards the yolk sac receives quail stem cell emigrating from the embryo.
- Published
- 1978
- Full Text
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20. Ability of chicken B cells from different compartments to respond to TNP-Ficoll.
- Author
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Edelman AS, Bhogal BS, Bell MK, and Thorbecke GJ
- Subjects
- Animals, Animals, Newborn, B-Lymphocytes classification, B-Lymphocytes cytology, Cell Differentiation, Chickens, Ficoll analogs & derivatives, Immunization, Passive, Lymphocyte Activation, Thymectomy, B-Lymphocytes immunology, Bursa of Fabricius cytology, Ficoll immunology, Nitrobenzenes immunology, Polysaccharides immunology, Spleen cytology, Trinitrobenzenes immunology
- Abstract
The responsiveness of chicken B cells from various compartments to T-independent antigens was studied by immune transfers of spleen and bursa cells into immunosuppressed recipients. Bursa cells from 8- to 10-wk-old donors failed to respond to trinitrophenylated Ficoll (TNP-F) even when thymus cells or splenic T cells were added. Spleen cells from the same donors transferred responses, as judged both by anti-TNP plaque-forming cells (PFC) per spleen and serum anti-TNP titers. In contrast, responses to TNP-Brucella abortus (TNP-BA) were transferred at least as well as by bursa as by spleen cells. Rabbit anti-chicken T cell serum plus complement treatment of the spleen cells reduced their ability to transfer responses to sheep erythrocytes, but either did not affect or enhanced serum antibody responses to TNP-BA and TNP-F. In intact animals, responsiveness to i.v. injected TNP-F was found to develop slowly after hatching in the chicken. At the age of 2 and 3 mo, PFC/spleen on day 4 after TNP-F injection were only 20% and 40%, respectively, of the adult response. Thymectomy at hatching further delayed this development, resulting in 12% and 45% of the adult control response at ages of 3 and 4 mo. It is concluded that responsiveness to the TI-2 antigen, TNP-F, develops slower than that to the TI-1 antigen, TNP-BA, and is restricted to the splenic B cell compartment. In addition, this development appears to be faster in the presence rather than in the absence of the thymus. In view of the previously shown effect of thymus on bursa development, these data suggest that the maturation of TI-1 antigen (TNP-F)-respondent chicken B cells requires residence in both the bursa and spleen before the development of responsiveness to such antigens.
- Published
- 1985
21. Studies of the chicken immune response. I. Correlation of the cellular and humoral immune response.
- Author
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Abramoff P and Brien NB
- Subjects
- Animals, Bursa of Fabricius cytology, Erythrocytes, Hemagglutination Tests, Histological Techniques, Sheep, Spleen cytology, Staining and Labeling, Thymus Gland cytology, Antibody Formation, Bursa of Fabricius immunology, Chickens immunology, Spleen immunology, Thymus Gland immunology
- Published
- 1968
22. Effect of inoculation of bursa cells on the splenic lymphoid tissue of bursectomized and x-irradiated chickens.
- Author
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Hoshi H
- Subjects
- Animals, Bursa of Fabricius cytology, Bursa of Fabricius radiation effects, Chickens, Injections, Intralymphatic, Lymphoid Tissue cytology, Lymphoid Tissue pathology, Male, Plasma Cells, Radiation Effects, Transplantation, Autologous, Transplantation, Homologous, Bursa of Fabricius transplantation, Spleen cytology
- Published
- 1972
- Full Text
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23. Antigen binding cells in embryonic chicken bursa and thymus.
- Author
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Dwyer JM and Warner NL
- Subjects
- Animals, Autoradiography, Bursa of Fabricius cytology, Bursa of Fabricius embryology, Chick Embryo, Phagocytosis, Salmonella immunology, Spleen cytology, Spleen embryology, Thymus Gland cytology, Thymus Gland embryology, Antigens, Bursa of Fabricius immunology, Lymphocytes immunology, Spleen immunology, Thymus Gland immunology
- Published
- 1971
- Full Text
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24. Migration of bone marrow cells to the bursa of Fabricius and the spleen in the chicken.
- Author
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Bäck O, Bäck R, Hemmingsson EJ, Lidèn S, and Linna TJ
- Subjects
- Animals, Autoradiography, Bone Marrow physiology, Bursa of Fabricius cytology, Cecum cytology, Chickens, Lymph Nodes cytology, Male, Scintillation Counting, Spleen cytology, Thymidine metabolism, Thymus Gland cytology, Tritium, Bone Marrow metabolism, Bone Marrow Cells, Bursa of Fabricius metabolism, Cecum metabolism, Lymph Nodes metabolism, Spleen metabolism
- Published
- 1973
- Full Text
- View/download PDF
25. Lymphocyte fine structure in the bursa of fabricius, the thymus, and the germinal centers.
- Author
-
Clawson CC, Cooper MD, and Good RA
- Subjects
- Animals, Antigen-Antibody Reactions, Basement Membrane, Cell Differentiation, Chromosomes, Cytoplasm analysis, Endoplasmic Reticulum, Golgi Apparatus, Mitochondria, Plasma Cells, Poultry, Ribosomes analysis, Bursa of Fabricius cytology, Lymphocytes cytology, Spleen cytology, Thymus Gland cytology
- Published
- 1967
26. [Histological studies on lymphatic organs in the chicken (Gallus domesticus) in the first year of age].
- Author
-
Hoffmann-Fezer G
- Subjects
- Animals, Bursa of Fabricius cytology, Cecum cytology, Cecum growth & development, Cell Count, Cell Nucleolus, Cell Nucleus, Chickens growth & development, Chromatin, Inclusion Bodies, Lymph Nodes cytology, Lymph Nodes growth & development, Plasma Cells, Spleen cytology, Thymus Gland cytology, Thymus Gland growth & development, Bursa of Fabricius growth & development, Lymphatic System growth & development, Spleen growth & development
- Published
- 1973
27. Cellular immune competence of spleen bursa and thymus cells.
- Author
-
Cain WA, Cooper MD, and Good RA
- Subjects
- Animals, Birds, Bursa of Fabricius cytology, Chickens, DNA metabolism, Hot Temperature, Lymphocytes metabolism, Lymphoid Tissue, RNA metabolism, Spleen cytology, Thymus Gland cytology, Bursa of Fabricius immunology, Spleen immunology, Thymus Gland immunology
- Published
- 1968
- Full Text
- View/download PDF
28. Histometric analysis of the chicken spleen during primary immune response to soluble bovine serum albumin. I. Relationship between quantitative changes of thymus-type and bursa-type splenic lymphocytes and splenomegaly.
- Author
-
Nagy ZA and Fehér G
- Subjects
- Animals, Bursa of Fabricius cytology, Cecum immunology, Chickens, Lymphocyte Activation, Plasma Cells immunology, Serum Albumin, Bovine, Splenomegaly immunology, Antibody Formation, B-Lymphocytes immunology, Spleen cytology, T-Lymphocytes immunology
- Published
- 1972
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