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The paper focuses on the Pleistocene deposits in Perspektywiczna Cave, southern Poland, related to cave hyena (Crocuta crocuta). We used direct radiocarbon dating of hyena fossils supported by genetic and stable isotope analyses to infer the paleobiology of this population. Radiocarbon dating of 19 hyena remains suggests long inhabitation of the region during early MIS 3, around 50–34 ky cal BP. The youngest among our dates, 34,355–33,725 cal BP (1σ, combined of two dates for the same specimen) points out the latest appearance of a cave hyena north to Carpathians. Beside this long period of occupation, the Perspektywiczna Cave hyenas stayed ecologically stable, but their genetic structure changed. Two mtDNA haplogroups were present, one typical for other Late Pleistocene European populations and the other one known so far only from recent African populations. [ABSTRACT FROM AUTHOR]

The domestic cat is the world's most popular pet and one of the most detrimental predators in terrestrial ecosystems. Effective protection of wildlife biodiversity demands detailed tracking of cat trophic ecology, and stable isotopes serve as a powerful proxy in dietary studies. However, a variable diet can make an isotopic pattern unreadable in opportunistic predators. To evaluate the usefulness of the isotopic method in cat ecology, we measured C and N isotope ratios in hundreds of archaeological cat bones. We determined trends in cat trophic paleoecology in northern Europe by exploiting population-scale patterns in animals from diverse locations. Our dataset shows a high variability of isotopic signals related to the socio-economic and/or geomorphological context. This points toward regularities in isotopic patterns across past cat populations. We provide a generalized guide to interpret the isotopic ecology of cats, emphasizing that regional isotopic baselines have a major impact on the isotopic signal. [ABSTRACT FROM AUTHOR]

Cat remains from Poland dated to 4,200 to 2,300 y BCE are currently the earliest evidence for the migration of the Near Eastern cat (NE cat), the ancestor of domestic cats, into Central Europe. This early immigration preceded the known establishment of housecat populations in the region by around 3,000 y. One hypothesis assumed that NE cats followed the migration of early farmers as synanthropes. In this study, we analyze the stable isotopes in six samples of Late Neolithic NE cat bones and further 34 of the associated fauna, including the European wildcat. We approximate the diet and trophic ecology of Late Neolithic felids in a broad context of contemporary wild and domestic animals and humans. In addition, we compared the ecology of Late Neolithic NE cats with the earliest domestic cats known from the territory of Poland, dating to the Roman Period. Our results reveal that human agricultural activity during the Late Neolithic had already impacted the isotopic signature of rodents in the ecosystem. These synanthropic pests constituted a significant proportion of the NE cat's diet. Our interpretation is that Late Neolithic NE cats were opportunistic synanthropes, most probably free-living individuals (i.e., not directly relying on a human food supply). We explore niche partitioning between studied NE cats and the contemporary native European wildcats. We find only minor differences between the isotopic ecology of both these taxa. We conclude that, after the appearance of the NE cat, both felid taxa shared the ecological niches. [ABSTRACT FROM AUTHOR]

Climate warming and human landscape transformation during the Holocene resulted in environmental changes for wild animals. The last remnants of the European Pleistocene megafauna that survived into the Holocene were particularly vulnerable to changes in habitat. To track the response of habitat use and foraging of large herbivores to natural and anthropogenic changes in environmental conditions during the Holocene, we investigated carbon (δ13C) and nitrogen (δ15N) stable isotope composition in bone collagen of moose (Alces alces), European bison (Bison bonasus) and aurochs (Bos primigenius) in Central and Eastern Europe. We found strong variations in isotope compositions in the studied species throughout the Holocene and diverse responses to changing environmental conditions. All three species showed significant changes in their δ13C values reflecting a shift of foraging habitats from more open in the Early and pre‐Neolithic Holocene to more forest during the Neolithic and Late Holocene. This shift was strongest in European bison, suggesting higher plasticity, more limited in moose, and the least in aurochs. Significant increases of δ15N values in European bison and moose are evidence of a diet change towards more grazing, but may also reflect increased nitrogen in soils following deglaciation and global temperature increases. Among the factors explaining the observed isotope variations were time (age of samples), longitude and elevation in European bison, and time, longitude and forest cover in aurochs. None of the analysed factors explained isotope variations in moose. Our results demonstrate the strong influence of natural (forest expansion) and anthropogenic (deforestation and human pressure) changes on the foraging ecology of large herbivores, with forests playing a major role as a refugial habitat since the Neolithic, particularly for European bison and aurochs. We propose that high flexibility in foraging strategy was the key for survival of large herbivores in the changing environmental conditions of the Holocene. [ABSTRACT FROM AUTHOR]

The Cave Bear, Ursus spelaeus (sensu lato), was one of many megafaunal species that became extinct during the Late Pleistocene in Europe. With new data we revisit the debate about the extinction and paleoecology of this species by presenting new chronometric, isotopic and taphonomic evidence from two Palaeolithic cave bear sites in northeastern Italy: Paina Cave and Trene Cave. Two direct radiocarbon dates on well-preserved collagen have yielded ages around 24,200-23,500 cal yr BP, which make them the latest known representatives of the species in Europe. The carbon (δ13C) and nitrogen (δ15N) isotopic values of bone collagen exhibit values similar to those of older cave bears from Swabian Jura and France, suggesting that the feedings preferences of cave bears remained unchanged until the disappearance of this species in Europe. Several bear remains preserved traces of human modification such as cut marks, which enables a reconstruction of the main steps of fur recovery and the butchering process. The broad range of plant types available and the favorable location of Berici Hills may have played an important role in the range expansion of cave bears and their interaction with the Paleolithic hunters settled the same area. [ABSTRACT FROM AUTHOR]

Higher δ15N values in bone collagen of mammoth (Mammuthus primigenius) compared with coeval large herbivores is a classic trait of the mammoth steppe. An exception applies to the Epigravettian site of Mezhyrich (ca. 18–17.4 ka cal BP) in the central East European plains, where mammoth bones have δ15N values equivalent to or in a lower range than those of horse specimens (Equus sp.). We expanded our preliminary dataset to a larger sampling size of mammoth, other large herbivores, and carnivores from contemporaneous and nearby sites of Buzhanka 2, Eliseevichi, and Yudinovo. The unusual low mammoth δ15N values were confirmed at Buzhanka 2 and for some specimens from Eliseevichi, while most individuals from Yudinovo displayed the expected high δ15N values, meaning similar to those of the large canids. The possibility of a contrast in migration pattern is not supported since the δ34S values, a marker of mobility, do not correlate with the δ15N values of mammoth bone collagen. No clear chronological tendency could be revealed, at least not at the scale of radiocarbon dating. The low range in δ15N values is likely to reflect a change in the specific niche of the mammoth in the southern part of its distribution. [ABSTRACT FROM AUTHOR]

We investigated how do environmental and climatic factors, but also management, affect the carbon (δ13C) and nitrogen (δ15N) stable isotope composition in bone collagen of the two largest contemporary herbivores: European bison (Bison bonasus) and moose (Alces alces) across Europe. We also analysed how different scenarios of population recovery- reintroduction in bison and natural recovery in moose influenced feeding habitats and diet of these two species and compared isotopic signatures of modern populations of bison and moose (living in human-altered landscapes) with those occurring in early Holocene. We found that δ13C of modern bison and moose decreased with increasing forest cover. Decreasing forest cover, increasing mean annual temperature and feeding on farm crops caused an increase in δ15N in bison, while no factor significantly affected δ15N in moose. We showed significant differences in δ13C and δ15N among modern bison populations, in contrast to moose populations. Variation in both isotopes in bison resulted from inter-population differences, while in moose it was mainly an effect of intra-population variation. Almost all modern bison populations differed in δ13C and δ15N from early Holocene bison. Such differences were not observed in moose. It indicates refugee status of European bison. Our results yielded evidence that habitat structure, management and a different history of population recovery have a strong influence on foraging behaviour of large herbivores reflected in stable isotope signatures. Influence of forest structure on carbon isotope signatures of studied herbivores supports the “canopy effect” hypothesis. [ABSTRACT FROM AUTHOR]

The late Middle Pleistocene archaeological site of Casal de' Pazzi (MIS 7, ∼240–200 ka) in central Italy provided a complex of paleontological (both fauna and flora) and archaeological evidence, as well as a cranial fragment of Homo heidelbergensis. Here, we investigated the stable carbon and oxygen isotopic ratios of tooth enamel from six herbivore species (Palaeoloxodon antiquus , Hippopotamus amphibius, Bos primigenius , Stephanorhinus kirchbergensis , Equus ferus , and Dama Dama) to contribute to the understanding of the paleoenvironment of the site through the reconstruction of the diet and habitat of Pleistocene mammals. Our results indicate that the analyzed taxa fed on C 3 plants and exploited both closed and open environments. This is consistent with the macro-botanical remains (leaf fossil impressions of Zelkova sp., Laurus nobilis , and Cercis siliquastrum) found at Casal de' Pazzi and pollen evidence from the nearby lake of Valle di Castiglione. The area around the site was characterized by diversified Mediterranean evergreen forest tree species, accompanied by mesophilic elements of the mixed deciduous oak and beach forest, alternating with diversified wooded/forested vegetation and xeric vegetation. This was the environmental context in which the late Middle Pleistocene Homo lived. Comparisons with published isotopic data from other European archaeological localities between ∼600 and 125 ka evidenced considerable environmental differences through time and space, according to the general climate trends and local factors, such as latitude, temperature, and vegetation composition. • Mammals from Casal de' Pazzi fed on C 3 plants in closed and open environments. • Between ∼600 and 125 ka, mammals occupied diverse ecological niches. • Homo heidelbergensis of Casal de' Pazzi lived in a mosaic C 3 -plant-dominated landscape. [ABSTRACT FROM AUTHOR]

The Gravettian site of Předmostí I in the central Moravian Plain has yielded a rich and diverse large mammal fauna dated around 25–27,000 14 C years BP (ca. 29,500–31,500 cal BP). This fauna includes numerous carnivores (cave lion, wolf, brown bear, polar fox, wolverine) and herbivores (reindeer, large bovine, red deer, muskox, horse, woolly rhinoceros, woolly mammoth) whose trophic position could be reconstructed using stable isotopic tracking (δ 13 C, δ 15 N, δ 34 S) of bone collagen ( n = 63). Among large canids, two morphotypes, “Pleistocene wolves” and “Palaeolithic dogs”, were considered, and two human bones attributed to the Gravettian assemblage of Předmostí I were also sampled. The trophic system around the Gravettian settlement of Předmostí I showed the typical niche partitioning among herbivores and carnivores seen in other mammoth-steppe contexts. The contribution of the analyzed prey species to the diet of the predators, including humans, was evaluated using a Bayesian mixing model (SIAR). Lions included great amounts of reindeer/muskox and possibly bison in their diet, while Pleistocene wolves were more focused on horse and possibly mammoth. Strong reliance on mammoth meat was found for the human of the site, similarly to previously analyzed individuals from other Gravettian sites in Moravia. Interestingly, the large canids interpreted as “Palaeolithic dogs” had a high proportion of reindeer/muskox in their diet, while consumption of mammoth would be expected from the availability of this prey especially in case of close interaction with humans. The peculiar isotopic composition of the Palaeolithic dogs of Předmostí I may indicate some control of their dietary intake by Gravettian people, who could have use them more for transportation than hunting purpose. [ABSTRACT FROM AUTHOR]

The Early Eocene deposits of El Kohol, Algeria, have yielded numerous remains of Numidotherium koholense, one of the most primitive and oldest known proboscideans in Africa. The Upper Eocene proboscideans of the Fayum locality (Egypt), Barytherium sp. and Moeritherium sp., were recently interpreted as aquatic or semi-aquatic, according to the stable isotopic compositions (&#94813C and &#94818O) of their tooth enamel. These data led us to reinvestigate the adaptations of N. koholense. Stable isotopic analysis and observations of histological sections of its long bones reveal that it was essentially terrestrial. According to its position within the phylogenetic tree of Eocene proboscideans, the adaptation to semi-aquatic life appears to have evolved independently in different lineages of Middle and Upper Eocene proboscideans during their adaptive radiation in Africa. Moreover, these new results reopen the debate about the hypothesis that Eocene to Recent proboscideans are derived from semi-aquatic ancestors. [ABSTRACT FROM AUTHOR]

We report here a new discovery of a cave bear left metatarsal 3 from Rochedane, an archaeological site near Montbeliard (French Jura) that yielded only Lateglacial and Holocene material, with no evidence of pre-LGM deposits, a context that made this bone a possible candidate for being a post-LGM cave bear in western Europe. To test this hypothesis, this bone was analyzed for mitochondrial DNA, which confirmed its attribution to cave bear of the Ursus spelaeus lineage, and a direct radiocarbon AMS dating on well preserved collagen (%C, %N and C/N well in the range of fresh collagen) yielded an age of 23,900 +110 −100 BP (28,730–28,500 cal BP, one sigma range). Its carbon and nitrogen isotopic values were similar to those of slightly older cave bears from the Swabian Jura, around 300 km to the East, suggesting that the ecological preferences of cave bears remained unchanged until the extirpation of this species in western Europe. Interestingly, the genetic type U. spelaeus was replaced by Ursus ingressus around 28,000 14 C BP in the Swabian Jura. In contrast, the older type U. spelaeus apparently persisted in France ca. 3000 years longer. Traces left on the cave bear metapodium have been left by human activity on this bone, as it was the case for older cave bear bones from the Swabian Jura. This case study shows that cave bear remains found in post-LGM sites or layers may be candidates to be late survivors of this extinct species, but without direct radiocarbon AMS dated on well-preserved collagen (demonstrated by actual chemical composition results) and ancient DNA confirmation of the species attribution, such evidence can only be considered dubious. [ABSTRACT FROM AUTHOR]

Stable isotope analyses provide one of the few means to evaluate diet of extinct taxa. However, interpreting isotope data from bone collagen of extinct animals based on isotopic patterns in different tissues of modern animal proxies is precarious. For example, three corrections are needed before making comparisons of recent hair and ancient bone collagen: calibration of carbon-13 variations in atmospheric CO2, different isotopic discrimination between diet–hair keratin and diet–bone collagen, and time averaging of bone collagen versus short-term record in hair keratin. Recently, Robu et al. [Isotopic evidence for dietary flexibility among European Late Pleistocene cave bears (Ursus spelaeus). Can J Zool. 2013;91:227–234] published an article comparing extant carbon (δ13C) and nitrogen (δ15N) stable isotopic data of European cave bear bone collagen with those of Yellowstone Park grizzly bear hair in order to test the prevailing assumption of a largely vegetarian diet among cave bears. The authors concluded that cave bears were carnivores. This work is unfortunately unfounded as the authors failed to consider the necessary corrections listed above. When these corrections are applied to the Romanian cave bears, these individuals can be then interpreted without involving consumption of high trophic-level food, and environmental changes are probably the reason for the unusual isotopic composition of these cave bears in comparison with other European cave bears, rather than a change of diet. We caution researchers to pay careful attention to these factors when interpreting feeding ecology of extinct fauna using stable isotope techniques. [ABSTRACT FROM AUTHOR]

Abstract: The Middle to Upper Palaeolithic transition around 35,000 years ago coincides with the replacement of Neanderthals by anatomically modern humans in Europe. Several hypotheses have been suggested to explain this replacement, one of them being the ability of anatomically modern humans to broaden their dietary spectrum beyond the large ungulate prey that Neanderthals consumed exclusively. This scenario is notably based on higher nitrogen-15 amounts in early Upper Palaeolithic anatomically modern human bone collagen compared with late Neanderthals. In this paper, we document a clear increase of nitrogen-15 in bone collagen of terrestrial herbivores during the early Aurignacian associated with anatomically modern humans compared with the stratigraphically older Châtelperronian and late Mousterian fauna associated with Neanderthals. Carnivores such as wolves also exhibit a significant increase in nitrogen-15, which is similar to that documented for early anatomically modern humans compared with Neanderthals in Europe. A shift in nitrogen-15 at the base of the terrestrial foodweb is responsible for such a pattern, with a preserved foodweb structure before and after the Middle to Upper Palaeolithic transition in south-western France. Such an isotopic shift in the terrestrial ecosystem may be due to an increase in aridity during the time of deposition of the early Aurignacian layers. If it occurred across Europe, such a shift in nitrogen-15 in terrestrial foodwebs would be enough to explain the observed isotopic trend between late Neanderthals and early anatomically modern humans, without any significant change in the diet composition at the Middle to Upper Palaeolithic transition. [Copyright &y& Elsevier]

Abstract: The subsistence patterns of Iron Age and Historical period humans from south-western Turkmenistan have been reconstructed using the carbon and nitrogen isotopic compositions of archaeological faunal and human bones. A qualitative comparison of the isotopic signatures points to a small proportion of ruminant meat and dairy in human diet for both periods. The ranges of proportions of dietary items yielded by a quantitative approach based on concentration dependent mixing models confirm the high proportions of plant food in the average diet, and show little change in the reconstructed diet for both periods. A comparison of results from zooarchaeological and isotopic approaches illustrates their complementarity in subsistence patterns reconstruction. [Copyright &y& Elsevier]

Abstract: The carbon and nitrogen isotopic abundances of the collagen extracted from the Saint-Césaire I Neanderthal have been used to infer the dietary behaviour of this specimen. A review of previously published Neanderthal collagen isotopic signatures with the addition of 3 new collagen isotopic signatures from specimens from Les Pradelles allows us to compare the dietary habits of 5 Neanderthal specimens from OIS 3 and one specimen from OIS 5c. This comparison points to a trophic position as top predator in an open environment, with little variation through time and space. In addition, a comparison of the Saint-Césaire I Neanderthal with contemporaneous hyaenas has been performed using a multi-source mixing model, modified from Phillips and Gregg (2003, Oecologia 127, 171). It appears that the isotopic differences between the Neanderthal specimen and hyaenas can be accounted for by much lower amounts of reindeer and much higher amounts of woolly rhinoceros and woolly mammoth in the dietary input of the Neanderthal specimen than in that of hyaenas, with relatively similar contributions of bovinae, large deer and horse for both predators, a conclusion consistent with the zooarchaeological data. The high proportion of very large herbivores, such as woolly rhinoceros and woolly mammoth, in Neanderthal''s diet compare to that of the scavenging hyaenas suggests that Neanderthals could not acquire these prey through scavenging. They probably had to hunt for proboscideans and rhinoceros. Such a prey selection could result from a long lasting dietary tradition in Europe. [Copyright &y& Elsevier]

Abstract: Measuring the amounts of nitrogen and carbon in small quantities of ancient bones before radiocarbon dating using elemental analysis is a way to quantify the amount of preserved collagen and the quantity of exogenous contaminating carbon. Such a measurement can be performed on a large number of samples and allows to sort out the specimens with the best preserved collagen, those with the most carbon contamination, and eventually to infer relationships between burial conditions and collagen preservation. This approach is most interesting as a preliminary method to use before attempting radiocarbon dating using accelerator mass spectrometry of bone collagen. [Copyright &y& Elsevier]

Trophic Enrichment Factor (TEF) is the main parameter used in isotopic trophic ecology. TEF values can be derived from specimens subjected to experimental feeding or from free-ranging specimens whose dietary behavior is well monitored, and it can be measured for different tissues of animal body. Direct collagen-to-collagen TEF is a key parameter for fossil material and needs to be well constrained in order to ascertain the reliability of the palaeodietary models. In this paper, we present isotopic results for a subfossil bone accumulation related to red fox ( Vulpes vulpes ) activity, discovered in an abandoned mine in Potok-Senderki (Poland). The objective was to report δ 13 C and δ 15 N collagen data for red foxes and their prey. These data were used to calculate a prey-predator collagen-to-collagen TEF and provided important information for interpreting stable isotope fractionation in terrestrial food webs. We used different taphonomic indexes to calculate the fox mean diet. The presence of juvenile and adult individuals of fox allowed us to specify the difference in isotopic enrichment according to the age class of the predator. Δ 13 C and Δ 15 N values calculated here for fox were similar to TEF values presented previously for wolf and lynx, but characterized by wider standard deviation. [ABSTRACT FROM AUTHOR]

Species interactions form food webs, impacting community structure and, potentially, ecological dynamics. It is likely that global climatic perturbations that occur over long periods of time have a significant influence on species interaction patterns. Here, we integrate stable isotope analysis and network theory to reconstruct patterns of trophic interactions for six independent mammalian communities that inhabited mammoth steppe environments spanning western Europe to eastern Alaska (Beringia) during the Late Pleistocene. We use a Bayesian mixing model to quantify the contribution of prey to the diets of local predators, and assess how the structure of trophic interactions changed across space and the Last Glacial Maximum (LGM), a global climatic event that severely impacted mammoth steppe communities. We find that large felids had diets that were more constrained than those of co-occurring predators, and largely influenced by an increase in Rangifer abundance after the LGM. Moreover, the structural organization of Beringian and European communities strongly differed: compared with Europe, species interactions in Beringian communities before--and possibly after--the LGM were highly modular. We suggest that this difference in modularity may have been driven by the geographical insularity of Beringian communities. [ABSTRACT FROM AUTHOR]

In this paper, stable isotope (δ 13C, δ 18O) analyses of five equid species from the Linxia Basin, northwestern China, were conducted to investigate dietary niche turnover during a critical Pliocene-Pleistocene phase in the evolution of Old World Equidae. In the Early Pliocene Shilidun fauna, Proboscidipparion pater is inferred to have mainly fed on C 3 grasses in open grasslands as indicated by their relatively high δ 13C values (−9.1 ± 0.4‰, five teeth, n = 61), whereas Cremohipparion licenti and Sivalhippus platyodus were possibly mixed feeders inhabiting woodlands and grasslands because of their comparatively low δ 13C values (−10.8 ± 0.6‰, seven teeth, n = 36; −10.6 ± 0.6‰, nine teeth, n = 35). Inhabiting the same environments, both C. licenti and S. platyodus went extinct, possibly, because climatic changes led to a decline in woodlands resulting in greater competition. In the Early Pleistocene Longdan fauna, Proboscidipparion sinense had higher δ 13C values (−9.1 ± 0.5‰, four teeth, n = 23) than coexisting Equus eisenmannae (−10.2 ± 0.5‰, eight teeth, n = 57), implying that P. sinense had a stronger grazing preference compared to E. eisenmannae. Ecomorphological analyses (body size, tooth crown height, and enamel surface complexity) reveal that P. pater and P. sinense had dental characteristics consistent with a strong grazing preference. Therefore, P. pater might have been preadapted to open grasslands during the warm and humid Early Pliocene, which helped its descendent P. sinense persist into the Pleistocene. The results of this study reveal distinct dietary niches for these equids for the first time and shed light on some aspects of the evolutionary history of Equidae in East Asia. • Dietary niche partitioning of five fossil equids were reconstructed through stable isotopes in East Asia for the first time. • Preadaption to open grasslands of Proboscidipparion pater during the Pliocene helped this genus persist into Pleistocene. • Cremohipparion licenti and Sivalhippus platyodus went extinct due to interspecific competition on habitats in Pliocene. • Early Pleistocene hipparion Proboscidipparion sinense occupied more open environments than coexisting Equus eisenmannae. [ABSTRACT FROM AUTHOR]

Oxygen isotopes are commonly applied to study archaeological human and animal mobility among the vertical ecological zones of the Central Andes in South America. Such research assumes that oxygen and hydrogen isotopes in meteoric waters demonstrate an inverse relationship with elevation. However, because the primary source of precipitation in the Central Andes is the Atlantic Ocean, this expectation is likely complicated by surface-level processes on the western Andean slope. We evaluate the spatial patterning of stable isotope values in surface waters along a coast-highland transect in southern Peru (∼15-17°S). Surface water δ18O and δ2H values in the study area are consistent with regional and global meteoric waters. However, lowland and highland surface waters demonstrate wide variability and overlapping ranges of surface water isotope values. Therefore, it is challenging to discern the origin of surface waters based on elevation alone. Rather, surface water δ18O and δ2H values appear to reflect hydrologic processes including seasonality, stream order, catchment size, and distance from the source. We identify the "Watershed Effect," which precludes the use of δ18O and δ2H in Andean bioarchaeological studies of inter-zonal mobility. Moreover, changing hydroclimate over the Holocene and present sampling precision for biological analytes confound existing interpretations of δ18O derived from archaeological bioapatite. Given the regional complexities of δ18O water presented here, isotopic assessments of human paleomobility require better baseline data than those currently available for the Central Andes. We contend that previous archaeological datasets using δ18O bioapatite to assess mobility between high and low elevations should be re-evaluated. Further, future studies should provide adequate baseline data to justify archaeological analyses and support subsequent interpretations. • New oxygen and hydrogen baseline data from surface water in the central Andes. • No clear correlation between δ 18O water or δ 2H water and elevation on the western slope. • Possible surface-level effects nclude catchment size, distance from the source, stream order, and seasonality. • Further interdisciplinary method development is required to assess paleomobility [ABSTRACT FROM AUTHOR]

Two archaeological sites, Tham Lod and Ban Rai rockshelters, in highland Pang Mapha, Mae Hong Son Province in northwestern Thailand have yielded several late Pleistocene to Holocene human and animal remains associated with the Hoabinhian technocomplex. Previously, stable carbon isotope compositions of human and faunal tooth enamel samples from Tham Lod Rockshelter have suggested a forest-grassland mosaic as being a Hoabinhian-related habitat in the region during the late Pleistocene. Although zooarchaeological data have implied rainforest specialization for early to mid-Holocene Hoabinhian hunter-gatherers, the extent and degree of human reliance on rainforest resources in the region have not yet been investigated in detail. To refine the timing of dietary changes and ecological adaptations of Hoabinhian hunter-gatherers in the region, we measured stable carbon isotope compositions of tooth enamel of humans and associated mammals from the early Holocene of Ban Rai Rockshelter and from several other Iron Age log-coffin sites in highland Pang Mapha, dated between 10,000 and 650 cal yr BP, in comparison with previously analyzed isotope data from the nearby late Pleistocene site of Tham Lod Rockshelter. The isotopic results from Ban Rai Rockshelter have revealed that the hunter-gatherers had a dietary shift to more exclusive C 3 food items starting at around the early Holocene or probably during the Pleistocene-Holocene transition, despite the availability of open canopies and no clear evidence of lithic technological changes. Since that time, a succeeding human subsistence strategy with more emphasized rainforest occupation, in response to more homogeneous and closed environments and wetter climate, has possibly remained unalterable in the region. This study documents the late emergence of specialized rainforest hunter-gatherers in the highland of northwestern Thailand, compared to archaeological findings in neighboring regions. Our findings highlight the asynchronous initialization of an ecological adaptation among hunter-gatherers as a reaction to environmental changes across different geographical regions during the late Pleistocene and Holocene. • Ecology of Holocene hunter-gatherers and associated faunas investigated in NW Thailand. • Hunter-gatherer habitats shifted to more closed environments in the early Holocene. • No lithic technological responses to changes during the latest Pleistocene-Holocene transition. • Rainforest occupation by hunter-gatherers emerging later in NW Thailand than surrounding regions. [ABSTRACT FROM AUTHOR]

Although camels are not indigenous to Europe, they have been found at several sites from several Roman provinces dating from the beginning of the 1st century AD onwards. It must have been beneficial to bring them there. Based on finds of remains from juvenile individuals (e.g. from Tanais), it has been suggested that the Romans might have systematically bred camels within Europe. For this study, we took serial samples of the enamel of four camels from European sites (Innsbruck-Wilten, Mamer-Bertrange, Tongeren, and Trier) dating to the 2nd - 4th century AD. We measured the relative abundances of carbon and oxygen isotopes of the carbonate fraction from the tooth enamel. The continuous record of oxygen and carbon isotopic composition of the intra-tooth enamel serial samples reflects the climate and habitat in which an individual lived during the time of tooth mineralization. We used these data to make a rough evaluation of the areas of origin consistent with the relative abundances of the isotopes from the enamel of the camels and attempt to reconstruct their life history and mobility behavior based on the different ecological characteristics of the habitats represented in the isotopic data. Furthermore, the data can function as an additional proxy for species determination, due to the different habitats of Camelus bactrianus and Camelus dromedarius. This work also yields interesting insights on the similarities in the mobility pattern of the camels from Mamer-Bertrange and Trier. In combination with archaeological evidence, it was possible to tentatively connect them with specific military units, i.e. the detachments of the Legio VIII Augusta. [ABSTRACT FROM AUTHOR]

Elephas maximus and Stegodon orientalis were two keystone proboscideans in southern Asia that coexisted mainly after the Middle Pleistocene in many regions. The long-term paleoecology and possible foraging competition of these two species have not been intensively investigated yet. Here, we applied stable isotope (C, O) analysis to the tooth enamel of coexisting Elephas maximus , Stegodon orientalis, and other associated mammalian species in Quzai Cave, southern China, dated to the early Late Pleistocene, to explore their paleoenvironmental context and foraging ecology. The δ 13C values of Elephas maximus were widely distributed between −17.9‰ and −11.9‰ (n = 10), while Stegodon orientalis δ 13C values ranged from −16.7‰ to −14.7‰ (n = 7). These results suggest that Elephas maximus was possibly a mixed feeder with a broader range of dietary resources than Stegodon orientalis, which probably browsed on a narrower range of plant resources in more densely forested landscape. A chronological comparison (from 8 Ma to recent) of published δ 13C data for these two species from Asia showed that none of them were dietary specialists. However, Elephas had a more flexible foraging ecology and a stronger ability to exploit abrasive grasses than Stegodon. The niche partitioning and perceived different foraging behaviors of Stegodon and Elephas might have reduced the level of interspecific competition and allowed them to coexist during the Pleistocene. Moreover, the high-level of ecological flexibility of Elephas might have helped them to survive until the present day, while Stegodon eventually went extinct by the terminal Pleistocene ∼12 ka. An extensive comparison and evaluation of the δ 13C data from fossil mammals in mainland southeast Asia during the Early to Late Pleistocene suggests that southern China was dominated by C 3 vegetation throughout the Pleistocene, in contrast with the evidence of C 4 biomes in neighboring Laos, Cambodia, and Thailand. Southern China experienced relatively stable environments during the Pleistocene, which can be attributed to the wide range of mountainous regions that acted as ecological refugia from human interference and climatic fluctuations, and allowed the preservation of high biodiversity. The isotopic data we present here provides new evidence about the ecological complexity of mainland southeast Asia and elucidates the need for more systematic research to investigate extinction models and ecological conservation in this region. • Stable isotopes reveal the foraging ecology of mammal fauna recovered from the Late Pleistocene Quzai Cave, southern China. • Elephas maximus was a mixed feeder in clear contrast with Stegodon orientalis who was restricted to browse in dense forest. • The highly dietary discrepancy between Elepha s and Stegodon might have resulted in their different destiny. • Southern China served as an ecological refugium sustaining a high biodiversity due to the wide mountainous region. • Southeast Asia displayed high ecological complexity with clear regional diversity. [ABSTRACT FROM AUTHOR]

Paleoecological and paleoclimatic records based on the stable isotopes of mammalian tooth enamel are poorly known in mainland Southeast Asia during the Pleistocene. Khok Sung in Northeastern Thailand is a late Middle Pleistocene terrace deposit, tentatively dated either as 213 ka or 188 ka, yielding 15 described mammalian taxa with especially abundant and complete fossil remains. To investigate paleodiets and habitats of these ancient mammals and to understand the corresponding regional climate, we performed an analysis of stable carbon and oxygen isotopes extracted from tooth enamel carbonate of various mammalian taxa, coupled with the cenogram method. The enamel δ 13 C values of Khok Sung mammals indicate a variety of diets, ranging from pure C 3 to C 4 plants, suggesting that C 4 grasses were a major component of local Thai ecosystems during the late Middle Pleistocene. The stable isotopic distinction between C 3 and C 4 plants suggests that the Pleistocene wildlife habitats ranged from closed forests to open grasslands for the Khok Sung area. Moreover, differences within sympatric Pleistocene herbivores such as proboscideans, rhinoceroses, and cervids characterize possible niche partitioning by minimizing interspecific overlap. Paleoclimatic interpretations based on the intra-tooth variability in enamel δ 18 O values from large mammals and on the cenogram analysis reflect significant seasonal variations in precipitation and temperature, and humid conditions, for the Khok Sung locality. Compared to modern environments in Thailand, it is apparent that C 4 -dominated grasslands were more widespread at that time when anthropic impacts on the ecosystems were presumably absent or minimal. [ABSTRACT FROM AUTHOR]

To access the annual isotopic variation of Toxodon platensis Owen, 1837, tooth enamel samples found in tanks in two localities in the Brazilian Intertropical Region (Puxinanã, Paraíba and Caruaru, Pernamuco) was analyzed isotopically (δ 13C, δ 18O). We suggest that one annual growth cycle of toxodonts could be represented by 11–17 mm of enamel. The carbon and oxygen isotopic values of the studied samples (LEG 2249–2253) are statistically different, interpreted as reflecting different climatic conditions in both localities. The interpretation of the carbon isotopic results shows little variation in the diet. The oxygen isotopic values of all samples represent the record of at least three years under different climate regimes. The carbon and oxygen isotopic values suggested that the feeding behaviour of these toxodonts were not influenced by the prevailing climatic conditions in both localities. • An seasonal isotopic diet for toxodonts is presented. • An year in the life of toxodonts is recorded at each 11–17 mm on enamel. • The δ 18O of the toxodonts allows the interpretation of climatic annual variation. • The feeding behaviour of toxodonts was not influenced by the local climate. [ABSTRACT FROM AUTHOR]

The Late Pleistocene site “Troisième caverne” of Goyet (Belgium) has yielded the broadest set of Neandertal remains in North-Western Europe and is associated with a rich and diverse large mammal assemblage. We reconstructed the dietary ecology at the site using stable isotope tracking (δ 13 C and δ 15 N) of bone collagen. The δ 13 C and δ 15 N values of all species are consistent with those observed in other “mammoth steppe” sites. The relative contribution of potential prey species to the diet of carnivores (including Neandertals) was evaluated using a Bayesian model. The distribution of individuals from herbivorous species and carnivorous ones was determined through cluster analysis in order to identify ecological niches, regardless of the individual species attribution. The Neandertals within the predator guild and the mammoth and reindeer as representatives of the herbivores occupied the most specific and most narrow ecological niches. The “Troisième caverne” of Goyet can be regarded as a key site for the investigation of Late Pleistocene Neandertal ecology north of the Alps. [ABSTRACT FROM AUTHOR]

The woolly mammoth ( Mammuthus primigenius ) was an emblematic and key species of the so-called mammoth steppe ecosystem between ca. 110,000 and 12,000 years ago. Its contribution to human subsistence during the Gravettian period as source of raw material was documented in southwestern France and southwestern Germany, with some evidence of active hunting in the latter region. However, decreasing genetic diversity and increasing indications of nutritional stress point to a likely decline of this megaherbivore. The specificity of the ecological niche occupied by the woolly mammoth is clearly reflected by their collagen 13 C and 15 N abundances (δ 13 C coll and δ 15 N coll ), measured on skeletal remains of the typical mammoth steppe. The abundances of carbon-13 in mammoth collagen are comparable to those of other grazers like horse ( Equus sp.), while the nitrogen-15 abundances are significantly higher (about 3‰) than in the other herbivores, either horse or reindeer ( Rangifer tarandus ). During the Aurignacian and Gravettian occupation at Geißenklösterle in the Ach Valley (Germany), the mammoths had the expected stable isotope signature, but the nitrogen-15 of horses showed an unexpected overlap with those of the mammoth. This unusual pattern was already occurring during the Aurignacian, while the oxygen-18 abundances in bone phosphate (δ 18 O bp ) of horse and reindeer were unchanged between Aurignacian and Gravettian periods, which rules out significant change in environmental and climatic conditions. Thus, we hypothesize that during the Aurignacian and Gravettian, the ecological niche of mammoth was intact but not occupied intensively by mammoths due to a decline in their population. This decline could be tentatively explained by human pressure through hunting. In Dordogne (France), decreasing horse and reindeer δ 15 N coll values coeval to decreasing horse δ 18 O bp values between the Aurignacian and the Early Gravettian periods reflected a clear change in the environment, while no contrast in δ 15 N coll values was observed between the Early and Final Gravettian at the Abri Pataud. The mammoth of Dordogne yielded slightly higher δ 15 N coll values than expected, probably as a consequence of the nursing effect since all the analyzed samples were ivory instead of bone. The direct dating and sulphur-34 measurement on the ivory of the Early Gravettian at Pataud showed that almost all of them were of contemporaneous and local origin. Significant contrasts in δ 34 S coll values were found between the Dordogne and the Ach Valley for the same herbivores species, which confirms the potential of sulphur-34 in collagen as a mobility tracker. [ABSTRACT FROM AUTHOR]

The woolly rhinoceros (Coelodonta antiquitatis) was a cold-adapted herbivore, widely distributed from western Europe to north-east Siberia during the Late Pleistocene. Previous studies have associated the extinction of the species ∼14,000 calendar years before present to climatic and vegetational changes, suggesting the later survival of populations in north-east Siberia may have related to the later persistence of open vegetation in the region. Here, we analyzed carbon (δ 13C) and nitrogen (δ 15N) stable isotopes and mitochondrial DNA sequences to elucidate the evolutionary ecology of the species. Our dataset comprised 286 woolly rhinoceros isotopic records, including 192 unpublished records, from across the species range, dating from >58,600 to 12,135 14C years before present (equivalent to 14,040 calendar years ago). Crucially, we present the first 71 isotopic records available to date of the 15,000 years preceding woolly rhinoceros extinction. The data revealed ecological flexibility and geographic variation in woolly rhinoceros stable isotope compositions across time. In north-east Siberia, we detected stability in δ 15N through time, which could reflect long-term environmental stability, and may have enabled the later survival of the species in the region. To further investigate the paleoecology of woolly rhinoceroses, we compared their isotopic compositions with other contemporary herbivores. Our findings suggested isotopic similarities between woolly rhinoceros and both musk ox (Ovibos moschatus) and saiga (Saiga tatarica), albeit at varying points in time, and possible niche partitioning between woolly rhinoceros and both horse (Equus spp.) and woolly mammoth (Mammuthus primigenius). To provide phylogeographic context to the isotopic data, we compiled and analyzed the 61 published mitochondrial control region sequences. The genetic data showed a lack of geographic structuring; we found three haplogroups with overlapping distributions, all of which showed a signal of expansion during the Last Glacial Maximum. Furthermore, our genetic findings support the notion that environmental stability in Siberia influenced the paleoecology of woolly rhinoceroses in the region. Our study highlights the utility of combining stable isotopic records with ancient DNA to advance our knowledge of the evolutionary ecology of past populations and extinct species. • First range wide study on woolly rhinoceros palaeoecology • First stable isotopes records from 15 kyr years pre-extinction. • Utility of merging stable isotope and ancient DNA to elucidate evolutionary history. • Ecology stability in NE Siberia enables late survival of the woolly rhinoceros. [ABSTRACT FROM AUTHOR]

Stable isotopes of oxygen and carbon of tooth enamel are increasingly being used as tracers to study palaeoecology and the diet preferences of fossil vertebrates. We serially sampled tooth enamel carbonate along the growth axes of five Early Maastrichtian carnivorous dinosaur teeth (Tarbosaurus bataar tyrannosaurid) from the Nemegt Formation, Mongolia, in order to identify seasonal climatic variations and determine the diet of this apex predator. Additional bulk samples of dentine, bone, and surrounding sediment were analyzed in order to exclude diagenetic obfuscation of the isotopic record. Enamel samples of potential prey species for dietary studies were also analyzed. In the case of the largest specimens, the sampled teeth usually recorded annual cycles ranging between two-thirds and a full year. Fluctuations in δ 18O values in tyrannosaurid teeth suggest seasonality (high annual temperatures with distinct precipitation/humidity maxima during summer months) with mean annual temperatures (MAT) ten degrees higher than those of present-day Mongolia. The seasonal pattern of δ 18O shows similarities to that of the modern-day Shijiazhuang Global Network of Isotopes in Precipitation station, northern China, and suggests that the Nemegt biota flourished when exposed to a cool temperate monsoon-influenced climate. Herbivore and carnivore carbon isotopes (δ 13C) values from tooth enamel imply the presence of a woodland ecosystem dominated by coniferous trees such as Araucariaceae, and are consistent with the hypothesis that large sauropods and hadrosaurids were the preferred prey of Tarbosaurus. Mean annual precipitation (MAP), based on the relationship between modern-day C3 gymnosperms and local average MAP, is estimated at 775–835 mm/yr. These results show that large theropod teeth can serve as valuable archives for palaeoenvironmental studies. • Stable isotopes in dinosaur enamel are used to infer paleobiology and paleoenvironment. • δ13C and δ18O in dinosaur enamel from Mongolia represent a pristine signature and provide information about diet and climate. • δ 13C values from tooth enamel imply that large sauropods and hadrosaurids were the preferred prey of Tarbosaurus. [ABSTRACT FROM AUTHOR]

The world's last population of woolly mammoths (Mammuthus primigenius) lived on Wrangel Island persisting well into the Holocene, going extinct at ca. 4000 cal BP. According to the frequency of radiocarbon dated mammoth remains from the island, the extinction appears fairly abrupt. This study investigates the ecology of the Wrangel Island mammoth population by means of carbon, nitrogen and sulfur isotope analyses. We report new isotope data on 77 radiocarbon dated mammoth specimens from Wrangel Island and Siberia, and evaluate them in relation to previously published isotope data for Pleistocene mammoths from Beringia and lower latitude Eurasia, and the other insular Holocene mammoth population from St. Paul Island. Contrary to prior suggestions of gradual habitat deterioration, the nitrogen isotope values of the Wrangel Island mammoths do not support a decline in forage quality/quantity, and are in fact very similar to their north Beringian forebears right to the end. However, compared to Siberian mammoths, those from Wrangel Island show a difference in their energy economy as judged by the carbon isotope values of structural carbonate, possibly representing a lower need of adaptive strategies for survival in extreme cold. Increased mid-Holocene weathering of rock formations in the central mountains is suggested by sulfur isotope values. Scenarios related to water quality problems stemming from increased weathering, and a possibility of a catastrophic starvation event as a cause of, or contributing factor in their demise are discussed. • No sign of habitat or forage deterioration in Wrangel Island mammoth isotope levels. • Different energy economy compared to Siberian mammoths. • Increased weathering in mid-Holocene possibly led to freshwater quality issues. • Short-term or catastrophic events are suggested as the cause of extinction. [ABSTRACT FROM AUTHOR]

Despite the explanation that the present-day biogeographic boundary between the Indochinese and Sundaic subregions at the Kra Isthmus, the narrowest part of Peninsular Thailand, in relation to differences in faunal and floral composition is almost conventionally valid for many groups of organisms, the distribution limits of modern and Pleistocene mammals in this region remain unclear. Moreover, environmental factors driving the past distribution patterns in a Southeast Asian mammal community and vegetation types contributing to the controversial hypothesis of an equatorial savanna corridor in the Thai-Malay Peninsula have rarely been demonstrated due to the scarcity of data. Since the discovery of a new Pleistocene fossil site, Yai Ruak Cave in Krabi Province, mammal fossils have been recovered from infilling sediments within the Permian karsts. On the basis of our first excavation, four mammal taxa: Hystrix cf. brachyura , Crocuta crocuta ultima , Rhinoceros sondaicus , and Rusa unicolor are taxonomically identified. A preliminary biochronological age ranging from the late Middle to early Late Pleistocene for the fauna based on the presence of C. c. ultima is proposed. The occurrence of C. c. ultima in this cave also represents the southernmost record of its known distribution, thus suggesting a non-concordant southern range limit of the Indochinese species at the Isthmus of Kra, but somewhere south of Krabi, during the Pleistocene. A bulk analysis of stable carbon isotopes investigated from tooth enamel of these mammals exhibits a variety of habitats ranging from pure C 3 to C 4 ecosystems, implying the existence of an open grassland landscape. The serial δ 18O data collected along the tooth crown heights of R. sondaicus possibly reflect no or little seasonal variation with high annual precipitation due to a major influence of both Southwest and Northeast monsoons in the region, similar to the modern climate. Unlike today, more open vegetation/forest-grassland mosaic was however dominant in Peninsular Thailand at that time. The southward distribution range of the spotted hyaena was likely limited by the discontinuous north-south savanna corridor, latitudinally separated by a transequatorial rainforest belt, starting around the northern part of Sundaland. The ecological distinction between closed and open canopy habitats in the Thai-Malay Peninsula remarkably played a major role in confining the southward distribution of some Indochinese species and in obstructing the migration of grassland-inhabiting taxa across the Sundaic subregion during the Pleistocene glacials. • A new fossil site has yielded a typical Pleistocene mammal fauna with the southernmost known range of spotted hyaenas. • Spotted hyaenas from Yai Ruak suggest a further southward biogeographic shift of the Pleistocene Indochinese-Sundaic species. • Peninsular Thailand served as a major migration pathway between the northern and southern mammal faunas. • Unlike today, a forest-grassland mosaic environment was present in the region at that time. • The lack of spotted hyaenas at lower than 9°N latitude was likely due to rainforest barriers along transequatorial regions. [ABSTRACT FROM AUTHOR]