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Grain protein content (GPC) is an important indicator of wheat quality. Earlier estimation of wheat GPC based on remote sensing provided effective decision to adapt optimized strategies for grain harvest, which is of great significance for agricultural production. The objectives of this field study are: (i) To assess the ability of spectral vegetation indices (VIs) of Sentinel 2 data to detect the wheat nitrogen (N) attributes related to the grain quality of winter wheat production, and (ii) to examine the accuracy of wheat N status and GPC estimation models based on different VIs and wheat nitrogen parameters across Analytical Spectra Devices (ASD) and Unmanned Aerial Vehicle (UAV) hyper-spectral data-simulated sentinel data and the real Sentinel-2 data. In this study, four nitrogen parameters at the wheat anthesis stage, including plant nitrogen accumulation (PNA), plant nitrogen content (PNC), leaf nitrogen accumulation (LNA), and leaf nitrogen content (LNC), were evaluated for their relationship between spectral parameters and GPC. Then, a multivariate linear regression method was used to establish the wheat nitrogen and GPC estimation model through simulated Sentinel-2A VIs. The coefficients of determination ( R 2 ) of four nitrogen parameter models were all greater than 0.7. The minimum R 2 of the prediction model of wheat GPC constructed by four nitrogen parameters combined with VIs was 0.428 and the highest R 2 was 0.467. The normalized root mean square error (nRMSE) of the four nitrogen estimation models ranged from 26.333% to 29.530% when verified by the ground-measured data collected from the Beijing suburbs, and the corresponding nRMSE for the GPC-predicted models ranged from 17.457% to 52.518%. The accuracy of the estimated model was verified by UAV hyper-spectral data which had resized to different spatial resolution collected from the National Experimental Station for Precision Agriculture. The normalized root mean square error (nRMSE) of the four nitrogen estimation models ranged from 16.9% to 37.8%, and the corresponding nRMSE for the GPC-predicted models ranged from 12.3% to 13.2%. The relevant models were also verified by Sentinel-2A data collected in 2018 while the minimum nRMSE for GPC invert model based on PNA was 7.89% and the maximum nRMSE of the GPC model based on LNC was 12.46% in Renqiu district, Hebei province. The nRMSE for the wheat nitrogen estimation model ranged from 23.200% to 42.790% for LNC and PNC. These data demonstrate that freely available Sentinel-2 imagery can be used as an important data source for wheat nutrition and grain quality monitoring. [ABSTRACT FROM AUTHOR]

• Our method focuses on exploring the effect of texture indices to estimate potato PNC. • An texture index was developed for PNC estimation throughout the potato growth cycle. • O-TTI3 can further improve the estimation accuracy of potato PNC. Plant nitrogen content (PNC) is vital for evaluating crop nitrogen nutrient status and for net primary productivity. Therefore, rapid and accurate acquisition of crop PNC information can help reduce fertilizer and increase efficiency in modern agriculture. This study investigated whether an optimized texture index constructed from hyperspectral characteristic wavelength images acquired from a unmanned aerial vehicle (UAV) may be used to estimate PNC in potatoes over multiple growth stages. A potato field trial conducted in 2019 in Beijing, China, included different planting densities, nitrogen fertilizer gradients, and potato varieties with three replicates. A UAV remote sensing platform served to acquire hyperspectral images during three critical N demand periods for potatoes. In addition, we simultaneously conducted field sampling to obtain ground-truth PNC measurements. Following the classical form of vegetation indices, 12 texture indices were constructed based on hyperspectral texture features, and an arbitrary variable combination optimization algorithm was used to optimize these indices. Finally, the texture index, which had the highest correlation with potato PNC, was used as the best indicator for estimating the PNC status of potato at multiple growth stages and whether this indicator, in combination with spectral information, could further improve the accuracy of potato PNC estimation was subsequently explored. The results showed that (i) the optimal texture index TTI3 (R 494 -Cor, R 578 -Hom, R 514 -Sem) maintained a good linear relationship with PNC at the late stage of potato growth, and the accuracy and stability of the PNC estimation models constructed based on it was significantly better than that of a single texture metric. (ii) Compared with spectral information alone, the texture index combined with spectral features improved the accuracy of potato PNC estimation. More specifically, TTI3 (R 494 -Cor, R 578 -Hom, R 514 -Sem) combined with the three-band spectral index TBI 5 (530, 734, 514) achieved the best estimation accuracy with calibrated R2 , root mean square error (RMSE), and normalized RMSE of 0.77%, 0.28%, and 9.88%, respectively. The results of this study showed that the texture index constructed by combining multiple texture metrics enhanced the association between texture features and potato PNC over multiple growth stages, thus improving the monitoring accuracy of potato nitrogen nutrition status. This study can provide a reference for accurately managing crop nitrogen nutrition. [ABSTRACT FROM AUTHOR]