1. Neural differentiation of embryonic stem cells induced by conditioned medium from neural stem cell.
- Author
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Zhang JQ, Yu XB, Ma BF, Yu WH, Zhang AX, Huang G, Mao FF, Zhang XM, Wang ZC, Li SN, Lahn BT, and Xiang AP
- Subjects
- Animals, Cell Count methods, Cell Proliferation drug effects, Cell Separation methods, Cells, Cultured, Embryo, Mammalian, Immunohistochemistry methods, Intermediate Filament Proteins metabolism, Mice, Mice, Inbred C57BL, Nerve Tissue Proteins genetics, Nerve Tissue Proteins metabolism, Nestin, Neuroglia drug effects, Neurons cytology, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction methods, Stem Cells physiology, Time Factors, Cell Differentiation drug effects, Culture Media, Conditioned pharmacology, Neurons drug effects, Stem Cells drug effects
- Abstract
Embryonic stem cells can proliferate indefinitely and are capable of differentiating into derivatives of all three embryonic germ layers in vitro, including the neural lineage. The main objective of this study is to test the effects of neural stem cell conditioned medium on the neural differentiation of mouse embryonic stem cells. When cultured in neural stem cell conditioned medium, mouse embryonic stem cells can form floating cell spheres composed of many nestin-positive cells. After trypsinization and growth on gelatin, these embryonic stem cell-derived neural progenitor cells can be expanded for more than 3 months without loss of neural progenitor characteristics. Both neuronal and glial cells can be readily generated from these cells under differentiation conditions. Thus, neural stem cell conditioned medium is a highly potent reagent for inducing the development of mouse embryonic stem cells into the neural lineage, especially neural progenitor cells.
- Published
- 2006
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