1. Topical tacrolimus does not negatively impact acute skin wound healing.
- Author
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Namkoong, Sun, Chung, Jimin, Yoo, Jiyeon, Jung, Minyoung, Gye, Jiwon, Kim, Ji Seok, Kim, Jee Young, Ahn, Sung Ku, Park, Byung Cheol, Kim, Myung Hwa, and Hong, Seung Phil
- Subjects
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TACROLIMUS , *SKIN wound treatment , *FULL-thickness wounds , *STEROID drugs , *MESSENGER RNA , *NEUTROPHILS , *HEALING - Abstract
Despite the increasing use of topical tacrolimus, there is little information about its effect on skin wound healing. To determine effects on acute cutaneous wound healing, two full-thickness skin wounds were imparted on the backs of 45 hairless mice, which were then divided into vehicle-, topical tacrolimus- and topical steroid-treated group. Each drug was topically applied once daily. The wound area was assessed by using dermoscopic images every two days after wounding. At 3, 7 and 11 days after wounding, 10 wounds in each group were collected for semi-quantitative analysis of histological features including re-epithelialization, polymorphonuclear leucocytes, fibroblasts and collagen. We also checked the mRNA expression levels of EGF, TGF-β, TNF-α and IL-1α. While topical application of clobetasol propionate was found to delay re-epithelialization and infiltration of polymorphonuclear leucocyte, topical treatment with tacrolimus showed patterns similar to that of the vehicle. In the tacrolimus-treated group, mRNA expression levels of IL-1α and TGF-β were slightly decreased, while the others were similar with the vehicle-treated group. Unlike steroid, topical tacrolimus, therefore, did not disturb the wound healing process in a murine skin wound model. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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