1. A keratinocyte and integrated fibroblast culture model for studying particulate matter-induced skin lesions and therapeutic intervention of fucosterol.
- Author
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Fernando IPS, Jayawardena TU, Kim HS, Vaas APJP, De Silva HIC, Nanayakkara CM, Abeytunga DTU, Lee W, Ahn G, Lee DS, Yeo IK, and Jeon YJ
- Subjects
- Air Pollutants adverse effects, Cells, Cultured, Coculture Techniques, Cytokines metabolism, Fibroblasts metabolism, Fibroblasts pathology, Humans, Inflammation etiology, Inflammation metabolism, Inflammation pathology, Keratinocytes metabolism, Keratinocytes pathology, NF-kappa B metabolism, Oxidative Stress, Reactive Oxygen Species metabolism, Skin pathology, Skin Diseases etiology, Skin Diseases metabolism, Skin Diseases pathology, Stigmasterol pharmacology, Fibroblasts drug effects, Inflammation drug therapy, Keratinocytes drug effects, Particulate Matter adverse effects, Skin drug effects, Skin Diseases drug therapy, Stigmasterol analogs & derivatives
- Abstract
Increased levels of particulate matter (PM) air pollutants in East Asia have resulted in detrimental health impacts increasing morbidity and mortality. Epidemiological studies suggest a possible relation between the cutaneous exposure of PM and increased oxidative stress and inflammation which lead to skin lesions. The present study utilizes an integrated cell culture model of keratinocytes and fibroblasts to mimic viable skin layers and investigate the possible effects of PM exposure after penetration through corneocytes. The skin perfection is upheld by homeostatic functionality of epidermal cells and the integrity of connective tissues. Exposure to xenobiotics could alter the skin cell homeostasis aggravating premature skin aging. Stimulation of HaCaT keratinocytes by PM collected from Beijing, China (CPM) increased the intracellular ROS levels triggering a cascade of events aggravating inflammatory responses and connective tissue degradation. In HDF fibroblasts, treatment with preconditioned keratinocyte culture media augmented inflammatory responses, cellular differentiation, and connective tissue degradation. Above events were marked by the increased intracellular ROS, inflammatory mediators, pro-inflammatory cytokines, matrix metalloproteinases (MMP)-1 and -2 levels, collagenase, and elastase activity. Fucosterol treatment of keratinocytes dose-dependently attenuated the detrimental effects both in keratinocytes and fibroblasts restoring the conditions near to physiological levels. Further evaluations could be advanced on developing fucosterol, in forms such as rejuvenating cosmeceuticals which could attenuate detrimental responses of CPM exposure., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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