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[Display omitted] • NCDFP-Cys based on a dual-ICT system possess longer fluorescence emisson at 717 nm and larger Stokes shift (Δλ = 167 nm). • NCDFP-Cys has been utilized to monitor the intracellular Cys fluctuation in pancreatic cancer cells during ferroptosis. • NCDFP-Cys reveal that there are distinction of Cys levels change in different activator-triggered ferroptotic pathways. Cysteine (Cys) as a crucial precursor for intracellular glutathione (GSH) synthesis, plays an important role in the redox regulation in ferroptosis, Therefore, evaluating intracellular Cys levels is worthy to better understand ferroptosis-related physiological process. In this work, we constructed a novel NIR coumarin-derived fluorescent probe (NCDFP-Cys) based on a dual-ICT system, the NCDFP-Cys can show fluorescence turn-on response at 717 nm toward Cys over other amino acids, and possess large Stokes shift (Δλ = 167 nm), low detection limit, hypotoxicity. More significantly, NCDFP-Cys has been utilized to monitor the intracellular Cys fluctuation in pancreatic cancer cells during ferroptosis induced by Erastin and RSL3 respectively, and revealing the difference of Cys levels changes in different activator-triggered ferroptosis pathways. [ABSTRACT FROM AUTHOR]

[Display omitted] • HL-Cys showed red-emission fluorescence and its Stokes shift was more than 100 nm. • HL-Cys with hypotoxicity and low detection limit (0.58 µM) was successfully used to the imaging of Cys in vitro and in vivo. In this work, a novel red-emission fluorescent probe (HL-Cys) based on benzothiazole moiety was designed and synthesized for the detection of cysteine (Cys). HL-Cys could display specific fluorescence response toward Cys over other analytes including amino acids and GSH at 620 nm (E x = 513 nm) because of the Michael addition reaction between Cys and HL-Cys, leading to the cleavage of acryloyl group from the parent ligand (HL). Moreover, HL-Cys with hypotoxicity and low detection limit was successfully used to the imaging of Cys in vitro and in vivo. [ABSTRACT FROM AUTHOR]

The probe plays a pivotal role in the early diagnosis of tumor, especially tumor microenvironment (TME)-responsive probe, which could exploit differences between tumor and normal tissue to achieve better signal-to-noise ratio. In view of overexpressed glutathione (GSH) in microenvironment considered as one of the important characteristics of tumor distinct from normal tissue, we constructed a GSH activated near-infrared fluorescent (NIRF) and photoacoustic (PA) dual-modal probe (Probe-GSH) based on the hybrid of xanthene-based hemicyanines and dicyanoisophorone. The activated Probe-GSH could exhibit 8-fold PA signal/10-fold NIRF intensity enhancement. Besides, compared with the existing xanthene-based hemicyanines (XBH), Probe-GSH possessed longer absorbance/emission wavelength and larger Stokes shift. More importantly, Probe-GSH has been successfully applied to the image of 4T1-bearing tumor mice under NIRF and PA dual modality. [Display omitted] • Compared with the reported xanthene-based hemicyanines, Probe-GSH possess longer the absorbance/ emission wavelength. • Compared with the reported xanthene-based hemicyanines, Probe-GSH has larger Stokes shift. • Probe-GSH was capable for imaging of tumor mouse under NIRF and PA dual modality image. [ABSTRACT FROM AUTHOR]

[Display omitted] • The excitation /emission wavelength of NIR dicyano-based fluorophoreare more than 650 nm. • The stokes shift of the NIR dicyano-based fluorophore is more than 100 nm. • Probe-Cys was used to visualize Cys in PC3 cells and PC3-xenograft tumor mice. • Probe-H 2 S has been applied in monitoring H 2 S releasing from the prodrug in vitro and in vivo. Near-infrared (NIR) fluorophores, especially dicyano-based fluorophores and xanthene-based hemicyanines, have been put high expectation in bioimaging application due to their excellent optical properties. However, they suffer from inherent shortages such as short excitation/emission wavelength (less than 700 nm) or small Stokes shift (20–50 nm). Herein, we constructed a novel NIR dicyano-based fluorophore (DCO-HBTN). To our knowledge, it is the first reported dicyano-based fluorophore of which the excitation/emission wavelength is more than 650 nm and Stokes shift is more than 100 nm. To demonstrate the feasibility of our efforts, we developed two NIR fluorescent probes (Probe-Cys and Probe-H 2 S) based on the fluorophore, Probe-Cys displayed good selective and highly sensitive (LOD = 0.28 μM) recognition of Cys over Hcy and GSH, which was used to visualize endogenous Cys in tumor tissue. Probe-H 2 S exhibited an. excellent specific and sensitive (LOD = 0.11 μM) response to H 2 S, which was applied in monitoring H 2 S releasing from the prodrug in vitro and in vivo. [ABSTRACT FROM AUTHOR]