1. The rgg Gene is a Specific Marker for Streptococcus oralis
- Author
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Hee Kuk Park, E.-G. Jeong, Hee Joong Lee, Won Joong Kim, and Hyung-Shik Shin
- Subjects
DNA, Bacterial ,Genetic Markers ,Sequence analysis ,Streptococcus mitis ,Biology ,Microbiology ,Bacterial Proteins ,Lactococcus ,Humans ,General Dentistry ,Gene ,DNA Primers ,Southern blot ,Genetics ,Streptococcus ,Streptococcus oralis ,Sequence Analysis, DNA ,biology.organism_classification ,Clone Cells ,Gram-Positive Cocci ,Blotting, Southern ,genomic DNA ,Streptococcus pneumoniae ,Suppression subtractive hybridization ,Genetic marker ,Trans-Activators ,Enterococcus ,Genome, Bacterial - Abstract
Although the pathogenesis of Streptococcus oralis may be different from that of other viridans group streptococci, S. oralis shares a high degree of DNA sequence similarity with these streptococci. As a result, discrimination of S. oralis from its close relatives has long been considered difficult. This study was conducted to find specific genes that allow for the in vitro identification of S. oralis, but not other oral commensals. Four hundred ninety S. oralis clones obtained by suppressive subtractive hybridization were used for Southern hybridization, and positive clones were sequenced. Of 5 S. oralis-specific clones, newly designed primer sets based on the glucosyltransferase regulatory gene amplified genomic DNA only from S. oralis strains, but not from any of the other 125 strains tested. Our findings may be useful for the future development of efficient diagnostic tools for the rapid identification and differentiation of S. oralis from other oral streptococci strains.
- Published
- 2010