1. Medium engineering for enhanced production of undecylprodigiosin antibiotic in Streptomyces coelicolor using oil palm biomass hydrolysate as a carbon source.
- Author
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Bhatia, Shashi Kant, Lee, Bo-Rahm, Sathiyanarayanan, Ganesan, Song, Hun-Seok, Kim, Junyoung, Jeon, Jong-Min, Kim, Jung-Ho, Park, Sung-Hee, Yu, Ju-Hyun, Park, Kyungmoon, and Yang, Yung-Hun
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UNDECYLPRODIGIOSIN , *STREPTOMYCES coelicolor , *OIL palm , *PLANT biomass , *WATER purification - Abstract
In this study, a biosugar obtained from empty fruit bunch (EFB) of oil palm by hot water treatment and subsequent enzymatic saccharification was used for undecylprodigiosin production, using Streptomyces coelicolor . Furfural is a major inhibitor present in EFB hydrolysate (EFBH), having a minimum inhibitory concentration (MIC) of 1.9 mM, and it reduces utilization of glucose (27%), xylose (59%), inhibits mycelium formation, and affects antibiotic production. Interestingly, furfural was found to be a good activator of undecylprodigiosin production in S . coelicolor , which enhanced undecylprodigiosin production by up to 52%. Optimization by mixture analysis resulted in a synthetic medium containing glucose:furfural:ACN:DMSO (1%, 2 mM, 0.2% and 0.3%, respectively). Finally, S . coelicolor was cultured in a fermenter in minimal medium with EFBH as a carbon source and addition of the components described above. This yielded 4.2 μg/mg dcw undecylprodigiosin, which was 3.2-fold higher compared to that in un-optimized medium. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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