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The transport of nitrogen (N) to groundwater and surface water in the form of nitrate (NO3-), as a by-product of the application of N-rich fertilisers, has been studied extensively. Yet, in the catchments adjacent to the Great Barrier Reef (GBR) in tropical north Queensland, Australia, NO3- transport in groundwater is not regularly monitored. An assessment of groundwater chemistry in the Liverpool Creek catchment of Queensland's wet-tropics region was conducted by regular sampling and analysis of groundwater over 12 months, through wet and dry seasons. A distinct spatial variability in groundwater chemistry was observed; groundwater aquifers with very low dissolved oxygen (DO) and NO3- consistently displayed relatively higher concentrations of sulphate (SO42-), sulphur (S2-) and ferrous iron (Fe2+) and low concentrations of dissolved organic carbon (DOC) (, Groundwater; nitrate; Sugarcane; Tropics, Sulphate; Denitrification; Soil Chemistry; Environmental Chemistry; Water Geochemistry; Contaminant Transport; Geochemistry, Environmental science.