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Your search keyword '"Millerioux, L"' showing total 5 results
Start Over Author "Millerioux, L" Topic suppressor factors, immunologic
5 results on '"Millerioux, L"'

1. Relation between a spleen-derived immunosuppressive peptide and the immunoglobulin-binding factor.

Author

Millerioux L, Duchange N, Masson A, and Lenfant M

Subjects
Animals, Binding Sites, Antibody, Chromatography, Affinity, Cytotoxicity, Immunologic drug effects, Immunoglobulin G metabolism, Immunoglobulin M biosynthesis, Immunoglobulin M metabolism, Mice, Peptides isolation & purification, Protein Binding, Antibody Formation drug effects, Immune Tolerance, Immunosuppressive Agents isolation & purification, Lymphokines isolation & purification, Peptides pharmacology, Suppressor Factors, Immunologic
Published
1981
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2. Relationship between a spleen-derived immunosuppressive peptide 'SDIP' and the 'Facteur thymique sérique' (FTS): biochemical and biological comparison of the two factors.

Author

Lenfant M, Millerioux L, Blazsek I, and Duchange N

Subjects
Animals, Antibody-Producing Cells drug effects, Chemical Phenomena, Chemistry, Physical, Chromatography, High Pressure Liquid, Electrophoresis, Lymphocyte Activation drug effects, Mice, Mice, Inbred Strains, Peptides isolation & purification, Immunosuppressive Agents pharmacology, Peptides pharmacology, Suppressor Factors, Immunologic, Thymic Factor, Circulating pharmacology, Thymus Hormones pharmacology
Abstract

A spleen-derived immunosuppressive peptide (SDIP) has been purified to homogeneity. Its physicochemical properties (electrophoretic mobility, u.v. spectra, absence of dansyl derivative) and its enzymatic susceptibilities (proteolytic enzymes, RNase, and DNase) were similar to those of the thymic hormone 'FTS'. SDIP and FTS were eluted with identical retention times in high performance liquid chromatography analysis in three different systems. When tested in sheep cell rosettes, and in the FTS radioimmunoassay in J.F. Bach's laboratory, SDIP presented an activity similar to FTS. In order to compare the thymic hormone to SDIP the biological activity of FTS was determined in in vivo and in in vitro humoral immunity reactions to a T-dependent antigen. As SDIP, FTS inhibited in vivo and in vitro the 19S-bearing cell formation during the last step of the differentiation of the lymphocytes, in the same range of concentration. The two factors appeared to stimulate the incorporation of [3H]-thymidine into the DNA of short-term cultures of thymocytes. The similarity of biological properties of SDIP and FTS together with the similarity observed in the physico-chemical and biochemical properties led to the conclusion that bovine spleen contains a factor similar to FTS.

Published
1983

3. Further study on the humoral response of a highly-purified spleen-derived immunosuppressive peptide (SDIP).

Author

Duchange N, Millerioux L, and Lenfant M

Subjects
Animals, Antigens, T-Independent immunology, Erythrocytes immunology, Immunoglobulin G immunology, Immunoglobulin M immunology, Immunologic Memory, Lymphocyte Activation drug effects, Lymphocytes metabolism, Mice, Mice, Inbred Strains, Spleen cytology, T-Lymphocytes immunology, Thymidine metabolism, Antibody Formation, Peptides immunology, Spleen immunology, Suppressor Factors, Immunologic
Abstract

Some biological properties of a highly-purified non-cytotoxic spleen-derived immunosuppressive peptide (SDIP) have been investigated. SDIP was shown to inhibit the primary anti-sheep red blood cell (SRBC) response at the last step of differentiation of the lymphocyte. In the present study we demonstrated that this response seemed to be T- and adherent spleen-cell dependent as no inhibition was noticed either in the response to TNP-LPS, a T-independent antigen, or in the response to SRBC in adherent spleen cell-depleted cultures. SDIP activity did not occur through an inhibition of splenocytes DNA synthesis since [3H]-thymidine ([3H]-TdR) incorporation was not modified in mitogen or allogenic stimulated cultures. Conversely, SDIP could act through a stimulation of a T-cell subset as a low specific increase of [3H]-TdR was noticed in cultured thymocytes.

Published
1981

4. Further study on the humoral response of a highly-purified spleen-derived immunosuppressive peptide (SDIP)

Author

Duchange, N, Millerioux, L, and Lenfant, M

Subjects
Erythrocytes, T-Lymphocytes, Mice, Inbred Strains, Lymphocyte Activation, Antigens, T-Independent, Mice, Immunoglobulin M, Immunoglobulin G, Antibody Formation, Suppressor Factors, Immunologic, Animals, Lymphocytes, Peptides, Immunologic Memory, Spleen, Research Article, Thymidine
Abstract

Some biological properties of a highly-purified non-cytotoxic spleen-derived immunosuppressive peptide (SDIP) have been investigated. SDIP was shown to inhibit the primary anti-sheep red blood cell (SRBC) response at the last step of differentiation of the lymphocyte. In the present study we demonstrated that this response seemed to be T- and adherent spleen-cell dependent as no inhibition was noticed either in the response to TNP-LPS, a T-independent antigen, or in the response to SRBC in adherent spleen cell-depleted cultures. SDIP activity did not occur through an inhibition of splenocytes DNA synthesis since [3H]-thymidine ([3H]-TdR) incorporation was not modified in mitogen or allogenic stimulated cultures. Conversely, SDIP could act through a stimulation of a T-cell subset as a low specific increase of [3H]-TdR was noticed in cultured thymocytes.

Published
1981

5. Relationship between a spleen-derived immunosuppressive peptide 'SDIP' and the 'Facteur thymique sérique' (FTS): biochemical and biological comparison of the two factors

Author

Lenfant, M, Millerioux, L, Blazsek, I, and Duchange, N

Subjects
Electrophoresis, Thymic Factor, Circulating, Chemical Phenomena, Chemistry, Physical, Mice, Inbred Strains, Lymphocyte Activation, Thymus Hormones, Mice, Suppressor Factors, Immunologic, Animals, Antibody-Producing Cells, Peptides, Chromatography, High Pressure Liquid, Immunosuppressive Agents, Research Article
Abstract

A spleen-derived immunosuppressive peptide (SDIP) has been purified to homogeneity. Its physicochemical properties (electrophoretic mobility, u.v. spectra, absence of dansyl derivative) and its enzymatic susceptibilities (proteolytic enzymes, RNase, and DNase) were similar to those of the thymic hormone 'FTS'. SDIP and FTS were eluted with identical retention times in high performance liquid chromatography analysis in three different systems. When tested in sheep cell rosettes, and in the FTS radioimmunoassay in J.F. Bach's laboratory, SDIP presented an activity similar to FTS. In order to compare the thymic hormone to SDIP the biological activity of FTS was determined in in vivo and in in vitro humoral immunity reactions to a T-dependent antigen. As SDIP, FTS inhibited in vivo and in vitro the 19S-bearing cell formation during the last step of the differentiation of the lymphocytes, in the same range of concentration. The two factors appeared to stimulate the incorporation of [3H]-thymidine into the DNA of short-term cultures of thymocytes. The similarity of biological properties of SDIP and FTS together with the similarity observed in the physico-chemical and biochemical properties led to the conclusion that bovine spleen contains a factor similar to FTS.

Published
1983

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